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To establish a method for simultaneous determination of neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，cynarin，galuteolin，isochlorogenic acid B，isochlorogenic acid A，isochlorogenic acid C in Shanjujiangya capsules by HPLC and analyze compositional change of Chrysanthemi Flos combined with law of quantity transfer．
The analysis was performed on Waters Symmetry C18 column (250 mm×4.6 mm，5 μm)，with mobile phase composed of acetonitrile -0.1% phosphoric acid solution at a flow rate of 1.0 mL·min-1 in gradient elution mode. The column temperature was 30 ℃ and the detection wavelength was 328 nm. The transfer rates of the above eight components were used as the indexes for quality evaluation to study the quantity value of transfer rule from the decoction piece to the extracting solution.
The results showed that the determination of eight components manifested a good linear relationship in the range of mass concentration (r>0.999 9)，the average recoveries of neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，cynarin，galuteolin，isochlorogenic acid B，isochlorogenic acid A，isochlorogenic acid C were 98.3%-101.9%，with RSDs of 0.066%-0.64%. The contents of the above 8 components in 3 samples were 0.257-0.279 mg·g-1，0.629-0.650 mg·g-1，0.402-0.476 mg·g-1，0.454-0.539 mg·g-1，1.118-1.278 mg·g-1，0.653-0.740 mg·g-1，0.659-0.706，1.138-1.167 mg·g-1，respectively.
The HPLC method established in this study is simple，repeatable and stable. The analysis of quantity transfer provides data support for the establishment of content methods and the formulation of limits. This study can provide basis for quality control method of Shanjujiangya capsules.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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