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To establish a quantitative analysis of multi-components by single marker (QAMS) for simultaneous determination of 7 flavanoids (5-hydroxy-6，7，3’，4’-tetramethoxyflavone，apigenin，hispidulin，kaempferol，jaceosidin，eupatilin and casticin) in Artemisiae Argyi Folium.
The HPLC system consisted of the Agilent ZORBAX SB-C18 column (150 mm×4.6 mm，5 μm) column with gradient elution of acetonitrile and 0.2% phosphoric acid as the mobile phase at a flow rate of 1.0 mL·min-1，a detection wavelength of 350 nm，and a column temperature of 30 ℃. Eupatilin was selected as the internal reference substance，the relative correction factors between eupatilin and the other 6 flavanoids were established，and the contents of these 7 constituents in samples were calculated to realize QAMS. At the same time，compared with the external standard method to verify the accuracy and feasibility of the QAMS method.
Within a certain linear range，the relative correction factors between eupatilin and 5-hydroxy-6，7，3’，4’-tetramethoxyflavone，apigenin，hispidulin，kaempferol，jaceosidin as well as casticin were 0.958，1.387，1.000，0.950，0.957 and 1.297，respectively (RSDs of RCFs were less than 2.0%). The contents of 5-hydroxy-6，7，3’，4’-tetramethoxyflavone，apigenin，hispidulin，kaempferol，jaceosidin，eupatilin，casticin in 20 batches of Artemisiae Argyi Folium were 0.031 4-0.623 5 mg·g-1，0.000 9-0.092 6 mg·g-1，0.020 6-0.170 7 mg·g-1，0.011 0-0.184 7 mg·g-1，0.011 7-0.864 0 mg·g-1，0.253 2-2.555 0 mg·g-1 and 0.015 6-0.250 7 mg·g-1，respectively.
Using eupatilin as the internal reference，QAMS method for 7 flavanoids is established. The method is accurate and reliable，and can be used for quality control and quantitative analysis of Artemisiae Argyi Folium.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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