To establish a high throughput method for the detection of 22 plant toxins in urine and blood by ultra performance liquid chromatography-triple quadrupole mass spectrometry.

The samples were extracted by acetonitrile oscillation, centrifuged, and the supernatant was purified by QuEChERS. Then, the matrix was matched with standard curve and quantified by external standard method. The full scanning mode of UPLC-MS /MS was used to quantitatively analyze and determine 22 kinds of target substances simultaneously. The influence of different extraction solvents, the selection and dosage of purification agent on sample extraction and purification by QuEChERS method was compared, and the sensitivity of the instrument was improved by optimizing the separation conditions of liquid chromatography and mass spectrometry parameters.

The regression equations of the 22 components to be measured in urine and blood had an outstanding linear relationship, with correlation coefficients were ≥0.996.The detection limits were 0.01～1.00 μg/L and 0.01～0.90 μg/L, the lower limits of quantification were 0.1-3.0 μg/L and 0.03-3.0 μg/L.The average recovery rates of spiked were 70.5%-125% and 73.7% -118%, Intraday precision and Daytime precision (RSD) were 1.2% to 15.2% (n=6) and 1.2% to 13.5% (n=6), respectively.

The method is rapid, accurate, sensitive and has high throughput. It can realize the rapid and efficient quantitative analysis of 22 plant toxins in blood and urine, and provide a reference for the determination oftrace plant toxins in biological samples.

To develop an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of eleven organophosphorus and ten neonicotinoid pesticides in vegetables.

Pesticides in sample were extracted with acetonitrile, cleaned up using a sorbent mixture of magnesium sulfate anhydrous, PSA and C₁₈. The separation was performed on a HSS T3 column. Detection was operated on multiple reaction monitoring scan under positive electrospray ionization. Matrix-matched external standard calibration curves were used for quantification.

The linear rang of calibration curves ranged from 0.50-100 ng/ml. Good linearities (r>0.999) were obtained for all 21 pesticides. The average recoveries were 76.0%-119.3% with RSD<10% for all the analytes. The limits of detection and quantification were 0.02-1.7 μg/kg and 0.06-5.7 μg/kg, respectively. This method was applied to analyze 76 vegetable samples from market. Nine target pesticides were detected in 26 samples, with a contents of 0.012-3.16 mg/kg.

This method is simple, sensitive, and accurate, and it is suitable for the determination of organophosphorus and neonicotinoid pesticide residues in vegetables.

To analyze and forecast the epidemic trends and disease burden of self-harm in China from 1990 to 2019, and to provide data for evaluating and formulating self-harm related prevention and treatment measures.

We used age-period-cohort modeling to estimate age, period, and cohort effects on self-harm incidence rates and mortality rates in China between 1990 and 2019. We projected the rates of self-harm in China from 2020 to 2030 using Bayesian age-period-cohort analysis.

In 2019, the age-standardized incidence rate of self-harm in China was 51.24/100 000 and the age-standardized mortality rate was 6.8/100 000. From 1990 to 2019, the disease burden of self-harm showed a decreasing trend in both male and female (EAPC<0). The results of APC analysis showed that in the age effect, the RR value of incidence peaked in 20-24 years old and over 70 years old, and the RR value of mortality increased rapidly in people over 70 years old.The period effect results showed that the overall morbidity and mortality risk of Chinese residents showed a downward trend from 1990 to 2019.Birth cohort results showed that the risk of morbidity and mortality for both male and female increased slowly and then decreased gradually with the change of birth year, and the population born between 1950 and 1954 had a higher risk of morbidity and mortality. By 2030, the ASIR of male self-harm is expected to decrease to 28.38/100 000 and that of female is 57.02/100 000. The ASMR of male decreased to 7.53/100 000 and that of female decreased to 3.90/100 000.

Although the burden of self-harm disease among Chinese residents shows an overall downward trend, it is still one of the diseases with a high burden of disease. Young people aged 20-24 and elderly people over 70 years old are targeted for life assistance and psychological counseling, and attention is paid to the physical and mental health of women who have attempted suicide, so as to reduce the burden of disease caused by self-harm.

To investigate the detection rate of osteosarcopenia in the elderly in the community of Shihezi city and to analyze its influencing factors.

From March to July 2023, a stratified random sampling method was used to select the elderly over 60 years old in Shihezi community for on-site questionnaire survey and physical measurement. Multivariate Logistic regression was used to analyze the influencing factors.

Among 1 051 elderly people, the detection rate of osteosarcopenia was 21.9% (230 people). Multivariate analysis showed that BMI (OR=0.78, 95% CI: 0.712-0.857), comorbidities (OR=1.774, 95% CI: 1.106-2.845), fracture history (OR=3.493, 95% CI: 1.748-6.979), duration of menopause (OR=1.080, 95% CI: 1.013-1.152), drinking strong tea OR coffee or carbonated drinks (OR=5.161, 95% CI: 2.731-9.752), sedentary inactivity (OR=1.899, 95% CI: 1.073-3.363), malnutrition (OR=4.601, 95% CI: 2.352-9.000), married (OR=0.041, 95% CI: 0.007-0.247), living alone (OR=3.404, 95% CI: 1.335-8.683), social frailty (OR=5.669, 95% CI: 3.852-10.674), low income (OR=2.248, 95% CI: 1.053-4.798) and employee medical insurance (OR=0.138, 95% CI: 0.037-0.510) and resident medical insurance (OR=0.023, 95% CI: 0.005-0.096) were the main influencing factor for the high-risk population of osteosarcopenia in the community.

The detection rate of sarcopenia and osteoporosis is high in the elderly in the community of Shihezi city. Community health workers should find the high-risk population of osteosarcopenia as soon as possible and take intervention measures.

To analyze and explore the association between lifestyle scores and the risk of developing nonalcoholic fatty liver disease (NAFLD) in the United States (US) population, and to provide new ideas for the prevention and treatment of NAFLD.

The samples of this study were collected from the 2007-2018 US National Health and Nutrition Examination Survey (NHANES) database. Logistic regression analysis was employed to assess the impact of healthy lifestyle and NAFLD. Further stratified analyses were conducted to investigate potential differences across gender, age, ethnicity, marital status, and education levels.

A total of 4 105 participants were included in this study, comprising 1 284 individuals with NAFLD (24.64%) and 2 821 individuals without NAFLD (75.36%). Compared to low lifestyle score, individuals with moderate (OR=0.372, 95% CI: 0.25-0.56, P=9.15×10^-6) and high lifestyle score (OR=0.091, 95% CI: 0.06-0.14, P=1.50×10^-17) had a lower risk of developing NAFLD. Stratified analyses revealed that this effect was more pronounced among males and individuals aged 60 and above.

Healthy lifestyle is associated with a decreased risk of NAFLD, and improving lifestyle score can be effective in preventing and treating NAFLD.

To systematically evaluate the post-market safety of human papilloma virus vaccine in Jiangxi province．

The doses number of HPV administered from 2017 to 2022 wascollected from all the vaccination institutions, and theadverse events followingimmunization（AEFI）cases were collected through the AEFI Surveillance Module of Chinese Disease Control and Prevention Information System. Using descriptive statistics, the incidences and epidemiological characteristics were estimated．

81 AEFI cases of HPV were reported from 2017 to 2022，and the incidence was 3.38 per 100 000 doses. Moreover, the incidence of HPV2, HPV4 and HPV9 were 3.21, 3.38 and 3.84 per 100 000 doses, respectively. A totalof 71.60% cases were 20-39 years old and 75.31%occurred in 2 days after inoculation. 59.26% were common adverse reactions. Most rare vaccine reactions were allergic rashes, and the reported incidence was 0.50 per 100 000 doses.

The human papilloma virus vaccine has acceptable safety profile in Jiangxi. The sensitivity of monitoring system needs to be further improved.

Development of the Lifestyle Health Beliefs Questionnaire for Patients with Polycystic Ovary Syndrome in Childbearing Age.

The first draft of the questionnaire was customized based on the health belief model dimensions and expert consultation; 273 patients with polycystic ovary syndrome in Chongqing were surveyed, and the data were tested for reliability and validity.

The total questionnaire Cronbach’s αvalue was 0.894, the individual dimensions Cronbach's α values ranged from 0.758 to 0.916, the questionnaire split-half reliability was 0.648, and the questionnaire re-test reliability was 0.772; six common factors were extracted from the exploratory factor analysis of the questionnaire for the 29 items, explaining 68.2% of the total variance, with S-CVI/Ave=0.944, and I-CVI for each dimension was 0.8 to 1. Validated factor analysis of the used questionnaire on lifestyle health beliefs in patients with polycystic ovary syndrome of childbearing age had good model fit, with discriminant validity ranging from 0.622 to 0.833, and combined reliability ranging from 0.748 to 0.901.

The developed questionnaire has good reliability and validity and is suitable for measuring lifestyle health beliefs in patients with polycystic ovary syndrome in reproductive age.

To analyze the prevalence of dementia among elderly people in China, to look into the factors that may influence it, and to consider the impact of the interplay between depression and reduced activity of daily living (ADL) function.

This study, which used data from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS), described the statistical status of dementia in 7 588 people over the age of 60 and explored the interaction between depression and different types of activities of daily living abilities on the prevalence of dementia based on logistic regression modelling.

High level of chronic disease co-morbidity (OR=1.634, 95% CI: 1.133-2.355), depression (OR=2.141, 95% CI: 1.505-3.044), impaired basic activity of daily living (BADL)(OR=5.576, 95% CI:4.249-7.317) and reduced instrumental activity of daily living (IADL) (OR=2.663, 95% CI: 1.738-4.082) were risk factors for dementia in older adults. There was an additive interaction between depressive symptoms and impaired BADL capacity. Older adults with depressive symptoms and reduced BADL performance had a 13.917-fold (OR=13.917, 95% CI: 8.694-22.279) increased chance of developing dementia.

Additive interaction between the depressive symptoms and impaired BADL capacity on the development of dementia, adopting effective steps to manage depressive symptoms and enhance BADL ability will help lower that risk.

To apply the case-based problem-based learning (PBL) teaching method in clinical and disease prevention education of obstetric and gynecological nursing and evaluate its effectiveness.

A total of 50 obstetric and gynecological nursing students were randomly divided into a control group and an experimental group. The control group received traditional teaching methods, while the experimental group received case-based PBL teaching method. After completing all teaching sessions, a comprehensive evaluation of student learning was conducted through performance assessment and survey questionnaires. The collected data were analyzed and visualized using SPSS statistical software.

Students taught using a case-based problem-based learning (PBL) approach demonstrated significantly higher scores in seven assessment indicators compared to students taught using traditional teaching methods (P<0.05). Among these indicators, the differences were most pronounced in clinical thinking ability (t=5.226, P<0.001) and self-directed learning ability (t=3.878, P<0.001). In terms of theoretical and practical scores, the experimental group had an average score of 88.68, which was higher than the control group’s average score of 85.92 (t=3.056, P=0.004).

The application of the case-based PBL teaching method in clinical and disease prevention education of obstetric and gynecological nursing effectively enhances students’ comprehensive abilities, particularly in improving their clinical thinking and self-directed learning abilities.

To analyze the prevalence of non-alcoholic fatty liver disease (NAFLD) and its influencing factors, and to explore the role of residual lipoprotein (RC) in risk assessment of NAFLD in the elderly.

The elderly over 60 years old in Xinzheng city, Henan Province in 2021 were selected as the research subjects, and the basic information, physical examination data, and laboratory indexes were collected. The role of RC in risk assessment of NAFLD in the elderly population was investigated by t test, χ² test, and multivariate Logistic regression analysis with the use of SPSS 26.0 software and R4.2.2.

A total of 62 730 elderly people were included in this study, and the prevalence rate of NAFLD was 26.02%. In univariate analysis, with the increase of RC level, the prevalence rate of the elderly population increased steadily, with the lowest in the first quartile and the highest in the second quartile (23.77% and 30.43%, respectively). Multivariate Logistic regression showed that the risk of NAFLD in women was 1.728 times higher than that in men (95%CI: 1.607-1.858), and the increase of age, BMI,waist circumference, fasting blood-glucose (FBG), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol(LDL-C), and RC increased the risk of NAFLD. For every unit increase in RC, the risk of disease increased by 56.30% (95%CI:1.433-1.704), and after adjusting for confounding factors, RC was still a risk factor for NAFLD.

The prevalence of NAFLD is high in the elderly, and there is an independent correlation between RC and NAFLD.