PDF
Full
Outline
To establish a high throughput method for the detection of 22 plant toxins in urine and blood by ultra performance liquid chromatography-triple quadrupole mass spectrometry.
The samples were extracted by acetonitrile oscillation, centrifuged, and the supernatant was purified by QuEChERS. Then, the matrix was matched with standard curve and quantified by external standard method. The full scanning mode of UPLC-MS /MS was used to quantitatively analyze and determine 22 kinds of target substances simultaneously. The influence of different extraction solvents, the selection and dosage of purification agent on sample extraction and purification by QuEChERS method was compared, and the sensitivity of the instrument was improved by optimizing the separation conditions of liquid chromatography and mass spectrometry parameters.
The regression equations of the 22 components to be measured in urine and blood had an outstanding linear relationship, with correlation coefficients were ≥0.996.The detection limits were 0.01～1.00 μg/L and 0.01～0.90 μg/L, the lower limits of quantification were 0.1-3.0 μg/L and 0.03-3.0 μg/L.The average recovery rates of spiked were 70.5%-125% and 73.7% -118%, Intraday precision and Daytime precision (RSD) were 1.2% to 15.2% (n=6) and 1.2% to 13.5% (n=6), respectively.
The method is rapid, accurate, sensitive and has high throughput. It can realize the rapid and efficient quantitative analysis of 22 plant toxins in blood and urine, and provide a reference for the determination oftrace plant toxins in biological samples.
PDF
51
20
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
