Objective To establish an ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of the characteristic components of Ophiopogon japonicus and Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma saponins B and Liriope muscari (Decne.) Baily saponins C in Xuanmai Ganju granules. Methods Xuanmai Ganju Granules were extracted by methanol ultrasound and analyzed using OMNI Orca${\mathrm{C}}_{18}$chromatography column (${2.1}\mathrm{\;{mm}}\times {100}\mathrm{\;{mm}},{2.6\mu }\mathrm{m}$). The mobile phase was eluted using a gradient elution of${10}\mathrm{{mmol}}/\mathrm{L}$ammonium acetate solution and methanol. The column temperature was set to${40}^{\circ}\mathrm{C}$, and the injection amount was${1\mu }\mathrm{L}$. The contents of methyl ophiopogon flavanone$\mathrm{A}$and methyl ophiopogon flavanone$\mathrm{B}$in Ophiopogon japonicus, as well as saponin B in Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma and saponin C in Liriope muscari (Decne.) Baily, using an electrospray ion source (ESI), a simultaneous scanning mode of positive and negative ions, and a multi reaction monitoring mode. Results Methyl ophiopogon flavanone A, methyl ophiopogon flavanone B, saponin B from Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma, and saponin C from Liriope muscari (Decne.) Baily have good linear relationships within their respective ranges, with $r$ values greater than 0.996 and recovery rates ranging from 90.6% to 99.8%. Conclusion This method has the advantages of simple and fast pre-treatment, high sensitivity, and accurate and reliable results. It can be used to determine the feeding situation of Ophiopogon japonicus mixed with Liriope spicata (Thunb.) Lour. in commercially available Xuanmai Ganju Granules and provide legal basis for regulatory authorities.