Objective To observe the test results and substantial impact of real-time fluorescence quantitative PCR detection technology in detecting hepatitis B virus DNA. Methods 1500 patients with hepatitis B from January 2023 to December 2023 were selected for the study data, and all of them were detected by serological markers and real-time fluorescent quantitative PCR. The control standard was the clinical diagnosis results, and the comparison index was the DNA quantitative detection results. Results Among the 1500 confirmed hepatitis B patients, real-time fluorescence quantitative PCR technology detected hepatitis B virus DNA quantification results, which showed that the viral load of 1380 patients (92.00%) could be clearly quantified, while the serological marker method could only clearly quantify the viral load of 1260 patients (84.00%). In addition to technology, factors that may affect the final test results include laboratory operations, sample and nucleic acid extraction, and storage at multiple stages. Conclusion Real time fluorescence quantitative PCR technology has higher sensitivity and accuracy compared to traditional serum blood marker detection methods, and is more suitable for modern laboratory medicine.

real time fluorescence quantitative PCR technology  /  hepatitis B virus  /  DNA quantitative detection  /  influence factor