Objective To explore the application effect of C-reactive protein combined with blood routine and blood culture in the diagnosis of bacteremia. Methods In this study, 69 patients with suspected bacteremia examined from May 2023 to May 2024 were selected as the observation group. In order to more intuitively understand the application value of the three combined detection methods, 69 healthy patients in the same period were selected as the experimental group. Analysis of blood routine indexes, strain distribution in blood culture and C-reactive protein detection data in both participating groups. Results In the blood cultures. Results A total of 8 strains were detected in the blood culture of 69 suspected bacteremia patients in the observation group. Among the two strains with high detection rate, except gram-negative bacteria and gram-positive bacteria, the two strains with the highest detection rate were staphylococcus 25 strains. Escherichia coli 16 granules, and 16 E. coli with detection rate of${23.19}\%$. WBC$\left({{15.45}\pm {2.18}}\right)\times {10}^{9}/\mathrm{L}$, NEU$\left({{81.24}\pm {2.84}}\right)\%$, LYM$\left({{53.24}\pm {2.68}}\right)\%$, C-reactive protein levels (53.24±2.68) mg/L, WBC (6.45±1.28)×10${}^{9}$/L, NEU (62.41±2.34)%, LYM (26.45±2.18)%, and C-reactive protein levels$\left({{6.28}\pm {1.25}}\right)\mathrm{{mg}}/\mathrm{L}$. WBC, NEU, LYM and C-reactive protein levels in patients with suspected bacteremia in the observation group were significantly higher than those of healthy patients in the experimental group$\left({P <{0.05}}\right)$. Of the 69 suspected bacteremia, 66 were positive and 3 were negative. Among them, 63 cases were positive in blood culture, 58 cases were positive in blood routine test, 56 cases were positive in C-reactive protein test, and 66 cases were positive in C-reactive protein combined with blood routine and blood culture test. Among the four detection methods, C-reactive protein combined with blood routine and blood culture test were no different with the confirmed results. Conclusion In clinical diagnosis and detection of suspected bacteremia patients, C-reactive protein combined with blood routine and blood culture can maximize the accuracy of detection data, and the combined diagnosis of the three is of higher clinical value.