Latest Articles18β-Glycyrrhetinic Acid, a naturally occurring active compound extracted from licorice, has recently garnered significant attention in the field of dermatological research. The pathophysiology of skin diseases is often highly complex, involving pathological processes such as inflammatory responses, oxidative stress, and cellular damage, which can severely impact patients' quality of life. Despite the availability of various treatment options, some medications are hampered by significant side effects or insufficient efficacy, highlighting the need for the development of safe and effective natural natural drugs. 18β-Glycyrrhetinic Acid stands out due to its remarkable pharmacological properties, including anti-inflammatory, antioxidant, wound-healing, and antitumor effects. Its ability to alleviate or improve symptoms of skin diseases through multiple mechanisms has led to widespread recognition of its biological activity and therapeutic potential. This review aims to summarize the research advancements on 18β-Glycyrrhetinic Acid in skin diseases, analyze its mechanisms of action, and discuss its clinical clinical prospects, thereby providing a scientific basis for future investigations and applications.
Due to their unique position, eyebrows can protect the eyes from external insults like dust, sweat, and pathogens. Eyebrows are also an important feature of facial aesthetics and identification, playing a significant role in beauty and social interactions. Sparse eyebrows not only severely affect the quality of life, but may also reflect underlying health issues. Hair loss is a widely concerned issue, but eyebrow hypotrichosis has not received enough attention yet. Currently, there is no standard evidence-based treatment for eyebrow hypotrichosis. Available treatment options mainly include medications, eyebrow transplants, and cosmetic concealment. This article reviews eyebrow physiology, the harm, causes, diagnosis, and treatment methods of eyebrow hypotrichosis, and the clinical application of bimatoprost in treating eyebrow hypotrichosis, aiming to providing references for the treatments for eyebrow hypotrichosis.
Atopic dermatitis is an immune-mediated inflammatory skin disease with a high recurrence rate. In traditional Chinese medicine (TCM) theory, it falls within the categories of "Siwan Feng" ("four-bend wind"), "Jinyin Chuang" ("soaking sore"), and "Nai Xuan" ("milk eczema"). Due to the risks to patient health associated with long-term use and the high cost of Western medicines, choosing treatment options has become challenging. In recent years, traditional Chinese medicine compound formulations have gained clinical attention due to their advantages such as causal treatment, regulation of constitution, and reduction of recurrence, showing great potential in the treatment of atopic dermatitis.Therefore, this article discusses the etiology and pathogenesis of atopic dermatitis in TCM and modern medical theory, the mechanism of TCM compound preparations for the treatment of atopic dermatitis, as well as the summary and outlook, aiming to provide a reference for the clinical treatment of atopic dermatitis.
To compare the antibacterial (against Escherichia coli/Salmonella), antiviral (against HSV-1/2), and NF-κB inhibitory activity of sea buckthorn leaf extracts (SBLE) from different origins.
SBLEs were prepared using 65% ethanol for hot reflux extraction of sea buckthorn leaves from different origins. The differences in their fingerprint profiles were analyzed by liquid chromatography. The colorimetric method was used to detect the inhibitory activity of Escherichia coli and Salmonella. The CCK-8 assay was employed to evaluate the activity of Vero cells infected with HSV-1/2. The luciferase reporter assay was conducted to assess the activity of NF-κB in TNF-α-induced NF-κB reporter (Luc)-HEK 293 cells.
SBLE from Datong and Huzhu exhibited better inhibitory effects on Escherichia coli (with EC50 values of 184.3 and 211.6 μg·mL-1, respectively) and Salmonella (with EC50 values of 284.6 and 300.9 μg·mL-1, respectively). SBLE from Datong, Pingan, Huzhu, Youyu, Heshun, and Zhangbei showed similar inhibitory effects on HSV-1 and HSV-2, with EC50 values ranging from 27 to 36 μg·mL-1. SBLE from Heshun, Zhangbei, and Huzhu exhibited better inhibition of NF-κB, with EC50 values of 122.6, 137.1, and 154.1 μg·mL-1, respectively.
The activities of SBLE from different origins vary, with SBLE from Datong exhibiting the best anti-Escherichia coli/Salmonella activity, SBLE from Youyu showing the best anti-HSV-1/2 activity, and SBLE from Heshun having the best NF-κB inhibitory effect.
To establish the quality standards for Jianpixiaoxian spray and provide a scientific basis for the optimization of its preparation process and quality control.
Jianpixiaoxian spray was prepared using a combination of steam distillation and water decoction methods. The quality of the product was preliminarily evaluated through pH value, filling volume variation, and spray volume. High-performance liquid chromatography (HPLC) was used to establish characteristic fingerprints for different batches, with neochlorogenic acid, chlorogenic acid, and 1, 3-dicaffeoylquinic acid used as marker components for multi-component content determination.
The prepared Jianpixiaoxian spray was a dark brown, clear, and transparent liquid with a fragrant aroma and a bitter taste. The pH value ranged from 6.2 to 7.4, with each bottle containing 9.5 to 10.5 mL and an average spray volume per actuation of 90.7 to 95.4 mg. The similarity of the HPLC fingerprints of 10 batches was above 0.90, with 13 common peaks identified. Among which Peaks 2, 4, and 8 were identified as neochlorogenic acid, chlorogenic acid, and 1, 3-dicaffeoylquinic acid, respectively. The contents of these three components were (0.314 7±0.0057) mg·mL-1, (0.626 3±0.009 1) mg·mL-1, and (0.170 9±0.003 2) mg·mL-1.
The optimized preparation process for Jianpixiaoxian spray is feasible. The established HPLC fingerprint and multi-component content determination methods are specific, repeatable, and stable, providing an effective means for quality control and evaluation of this preparation and laying a foundation for its clinical application and industrial production.
To establish a size exclusion high-performance liquid chromatography (SEC-HPLC) method for the determination of the purity of recombinant human basic fibroblast growth factor (rh-bFGF).
The purity of rh-bFGF solution was determined using TSK G2000SWXL column and high-performance liquid chromatography, and the specificity, repeatability, precision, detection limit, stability, and durability of the method were evaluated.
The blank solvent has no interference with the rh-bFGF main peak, and the degradation impurities under various destruction conditions didn't interfere with the determination of the rh-bFGF peak, and the purity index of the rh-bFGF peak was greater than 990. The repeatability and intermediate precision experimental results showed that the separation degree of impurities and the rh-bFGF main peak was greater than 1.5, and the RSD was 0.36% and 0.64%, respectively. The detection limit was 0.185 μg·mL-1. The durability experiment results show that fluctuations in flow rate, column temperature, and wavelength had no effect on the detection results, and the RSD of each condition was less than 2.0%.
The SEC-HPLC method developed has the advantages of simple operation, accurate results, strong specificity, high stability and good applicability, and can be used for rh-bFGF purity determination.
To investigate the residue of mycotoxins in Fukang tablets.
The multi-in-one immunoaffinity columns were used to establish a UPLC-MS/MS quantitative analysis method, which was applied to analyze 29 batches of samples collected from six manufacturing enterprises.
The linear relationship of 11 mycotoxins was good within their respective linear ranges, with the correlation coefficient r ranging from 0.996 4 to 0.999 9 and the limit of quantitation (LOQ) ranging from 0.4 to 40 ng/tablet. The average recovery rate of 11 mycotoxins was 74.0% to 115.7%, and the RSD ranged from 2.6% to 11.3%. Among 29 batches of Fukang Tablets, except one batch in which fumonisin B3 (FB3) was measured at 10.4 ng/tablet, while the mycotoxin levels in the other samples were all less than 5 ng/tablet.
This method is rapid, accurate, highly sensitive, and reproducible, making it suitable for the quantitative detection and safety evaluation of multiple mycotoxins in Fukang tablets with complex matrix components.
To establish a simple and economical high performance liquid chromatography (HPLC) method for determination of the concentrations of ceftazidime, meropenem, ceftriaxone and cefoperazone in human plasma.
The analysis was performed using an Agilent Extend C18 (4.6 mm × 250 mm, 5 μm) chromatographic column, and protein components were removed using ultrafiltration tubes. A gradient elution system consisting of acetonitrile and 50 mmol·L-1 sodium dihydrogen phosphate (pH 2.4) was employed as the mobile phase. The column temperature was maintained at 35 °C, with a flow rate of 1 mL·min-1. Detection was achieved using a dual-wavelength ultraviolet spectrophotometry at 250 and 300 nm.
The four β-lactam antibiotics exhibited good linearity within their respective ranges (r2> 0.999), with an average extraction recovery ranging from 68.98% to 83.50%. The intra-day precision RSD was between 0.06% and 2.67%, while the inter-day precision RSD was between 0.04% and 3.32%.
This study successfully established an HPLC method for the determination of the concentrations of ceftazidime, meropenem, ceftriaxone, and cefoperazone, which is suitable for monitoring the plasma concentrations of these four β-lactam antibiotics.
To develop a quantitative analysis of multi-components by single-marker (QAMS) method for simultaneous determination of six major components (nitidine chloride, chelerythrine, hesperidin, zanthoxylumol, dictamnine and pimpinellin) in Toddalia asiatica.
A reversed-phase high-performance liquid chromatography (HPLC) method was adopted using a Waters Symmetry C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile (containing 0.1% formic acid) with gradient elution. The flow rate was set at 0.8 mL·min-1, the detection wavelength was 280 nm, and the column temperature was maintained at 30 ℃. Taking hesperidin as the reference standard, relative correction factors (RCFs) of the other five components were determined, and the contents of the six major components were calculated. The accuracy and feasibility of the QAMS method were verified by investigating the robustness of RCFs and comparing the results with those obtained by the external standard method.
Within the linear range, the RCFs of nitidine chloride, chelerythrine, zanthoxylumol, dictamnine and pimpinellin were 0.170, 0.353, 3.443, 1.546 and 0.664, respectively. The RCFs exhibited good robustness under different experimental conditions (RSD < 3%). Additionally, there was no significant difference between the results obtained by the QAMS method and the external standard method for the analysis of eight batches of Toddalia asiatica.
The developed QAMS method is accurate and feasible, which can be used for determining the contents of major components in Toddalia asiatica.
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