PDF
Full
Outline
To develop a quantitative analysis of multi-components by single-marker (QAMS) method for simultaneous determination of six major components (nitidine chloride, chelerythrine, hesperidin, zanthoxylumol, dictamnine and pimpinellin) in Toddalia asiatica.
A reversed-phase high-performance liquid chromatography (HPLC) method was adopted using a Waters Symmetry C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile (containing 0.1% formic acid) with gradient elution. The flow rate was set at 0.8 mL·min-1, the detection wavelength was 280 nm, and the column temperature was maintained at 30 ℃. Taking hesperidin as the reference standard, relative correction factors (RCFs) of the other five components were determined, and the contents of the six major components were calculated. The accuracy and feasibility of the QAMS method were verified by investigating the robustness of RCFs and comparing the results with those obtained by the external standard method.
Within the linear range, the RCFs of nitidine chloride, chelerythrine, zanthoxylumol, dictamnine and pimpinellin were 0.170, 0.353, 3.443, 1.546 and 0.664, respectively. The RCFs exhibited good robustness under different experimental conditions (RSD < 3%). Additionally, there was no significant difference between the results obtained by the QAMS method and the external standard method for the analysis of eight batches of Toddalia asiatica.
The developed QAMS method is accurate and feasible, which can be used for determining the contents of major components in Toddalia asiatica.
PDF
141
60
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
