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To establish a simple and economical high performance liquid chromatography (HPLC) method for determination of the concentrations of ceftazidime, meropenem, ceftriaxone and cefoperazone in human plasma.
The analysis was performed using an Agilent Extend C18 (4.6 mm × 250 mm, 5 μm) chromatographic column, and protein components were removed using ultrafiltration tubes. A gradient elution system consisting of acetonitrile and 50 mmol·L-1 sodium dihydrogen phosphate (pH 2.4) was employed as the mobile phase. The column temperature was maintained at 35 °C, with a flow rate of 1 mL·min-1. Detection was achieved using a dual-wavelength ultraviolet spectrophotometry at 250 and 300 nm.
The four β-lactam antibiotics exhibited good linearity within their respective ranges (r2> 0.999), with an average extraction recovery ranging from 68.98% to 83.50%. The intra-day precision RSD was between 0.06% and 2.67%, while the inter-day precision RSD was between 0.04% and 3.32%.
This study successfully established an HPLC method for the determination of the concentrations of ceftazidime, meropenem, ceftriaxone, and cefoperazone, which is suitable for monitoring the plasma concentrations of these four β-lactam antibiotics.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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