To observe the clinical efficacy of Qingre Jiedu Decoction（清热解毒汤）in the treatment of severe psoriasis vulgaris（blood-heat type）and its impacton serum inflammation and immune function in patients.

A prospective study was conducted on 100 patients with psoriasis vulgaris who received treatment at Shengli Oilfield Central Hospital in Dongying City.They were divided into a control group and an observation group in the order of admission，with 50 patients in each group.The control group received conventional Western medicine treatment，while the observation group received oral administration with Qingre Jiedu Decoction for 8 weeks.The total effective rate，traditional Chinese medicine syndrome scores，serum inflammation level，immune function and adverse reactions between two groups were compared.

The total effective rate of the observation group（96.00%）was significantly better than that（84.00%）of the control group，and the difference was statistically significant（P<0.05）.Before intervention，there was no significant difference in skin lesions and quality of life scores between the two groups（P>0.05）.After8 weeks of intervention，compared with those of the controlgroup，the psoriasis area and severity index（PASI）and dermatology life quality index（DLQI）of the observation group were significantly reduced（P<0.01）.The scores of symptoms such as rash color，burning sensation，itching and irritability in the observation group significantly decreased（P<0.01）.There was no significant difference in serum inflammation or T lymphocyte subsets levels between the two groups before intervention.After 8 weeks of intervention，the levels of serum tumor necrosis factor-α（TNF-α），transforming growth factor-β（TGF-β）and interleukin-6（IL-6）of the observation group were significantly down-regulated compared to those of the control group（P<0.01）.The levels of CD3⁺，CD4⁺and CD4⁺/CD8⁺in the observation group were significantly increased compared to those in the control group，while the levels of CD8⁺were significantly decreased，with statistical significance（P<0.01，P<0.05）.The incidence of adverse reactions in the observation group（16.00%）showed no significant difference compared to that（10.00%）in the control group（P>0.05）.

The combination of Qingre Jiedu Decoction（Psoriasis External Wash No.1 Formula）and Avia Capsules can improve the clinical efficacy in patients with severe psoriasis vulgaris，further improve the clinical symptoms，enhance the quality of life and significantly reduce PASI，and the mechanism of action is related to inhibiting the body's inflammatory response and regulating immune function.

This study aimed to explore the clinical efficacy of Huanglian Wendan Decoction（黄连温胆汤）combined with acupoint application in the treatment of patients with dampness-heat of spleen and stomach type of functional dyspepsia（FD）and its impacton patients'quality of life and psychological well-being.

This study selected patients diagnosed as dampness-heat of spleen and stomach type of functional dyspepsia（FD）and treated at the hospital from October2021 to October 2023 as the research subjects，totaling 116 cases.According to the random number table method，they were divided into a control group（53 cases）and an experimental group（53 cases）.The patients in the control group received oral treatment with Mosapride Citrate Tablets，while those in the experimental group were treated with Huanglian Wendan Decoction combined with acupoint application on the basis of Mosapride Citrate Tablets.The efficacy of treatments and traditional Chinese medicine（TCM）syndrome scores were assessed and compared between the two groups.The levels of serum motilin（MTL），gastrin（GAS）and ghrelin before and after treatment were statistically compared.The Beck Depression Inventory（BDI），Beck Anxiety Inventory（BAI），Nipine Digestive Dysplasia Life Index Scale（NDLQL）and Medical Outcomes Study Health Survey Short Form-36 Item（SF-36）were used to evaluate the patients'quality of life and psychological state.Adverse reactions to the treatments in both groups were also recorded.

After treatment，the NDSI scores and TCM syndrome scores for primary and secondary symptoms in the experimental group were lower than those in the control group.Post-treatment levels of MTL and GAS decreased while ghrelin levels increased in both groups，with the experimental group showing lower levels ofMTL and GASand higher levels of ghrelin compared to the control group.The SF-36 scores for physical functioning（PF），role-physical（RP），bodily pain（BP），vitality（VT）and social functioning（SF）increased after treatment in both groups，with higher increases observed in the experimental group.The BDIand BAIscores decreased in both groups post-treatment，with the experimental group showing lower scores than the control group.The scores in all four domains of the NDLQI and the total scores increased after treatment in both groups，with the experimental group showing higher scores than the control group（P<0.05）.Compared to the control group，the experimental group had a significantly higher number of safety cases（P<0.05）.

Huanglian Wendan Decoction combined with acupoint application is an effective treatment for dampness-heat of spleen and stomach type of FD，and its further clinical application is warranted.

To investigate the effect of Zijinlong Tablets（紫金龙片）combined with intravenous immunoglobulin in the treatment of lower respiratory tract infection in the elderly.

Totally 302 elderly patients with lower respiratory tract infections admitted to the hospital from March 2021 to March 2023 were selected and randomly grouped，each with 151 cases.Based on conventional treatment，the control group was given intravenous immunoglobulin，and the study group received Zijinlong Tablets combined with intravenous immunoglobulin.Then the clinicalefficacy，ratio of forced expiratory volume in the firstsecond to forced vital capacity（FEV₁/FVC），peak expiratory flow（PEF），maximum voluntary ventilation（MVV），arterial partial pressure of oxygen（PaO₂），arterial partial pressure of carbon dioxide（PaCO₂），interleukin-6（IL-6），C-reactive protein（CRP），interleukin-1（IL-1），procalcitonin（PCT），immunoglobulin-（Ig）A，IgG，IgM and T-lymphocyte subsets（CD3⁺，CD4⁺，CD8⁺）were compared.

The study group reported a higher overall efficacy rate than the control group（93.38% vs85.43%，P<0.05）.An increase in PEF，MVV and FEV₁/FVC were observed in all patients after treatment，and the increase was more notable in the study group than those in the controlgroup（P<0.05）.An elevation in PaO₂ and a decrease PaCO₂ were detected in all patients，and both improvements were more remarkable in the study group than those in the control group（P<0.05）.After treatment，the levels of IL-6，CRP，IL-1 and PCT showed a decrease in all patients，and the three parameters were all lower in the study group than those in the control group（P<0.05）.The levels of IgA，IgG and IgM demonstrated an increase in all patients，and were all larger in the study group than those in the control group（P<0.05）.An elevation in CD3⁺and CD4⁺and a decrease CD8⁺were found in all patients，and both improvements were more obvious in the study group than those in the control group（P<0.05）.

The application of Zijinlong Tablets combined with intravenous immunoglobulin in the treatment of lower respiratory tract infection in the elderly can effectively improve the pulmonary function and arterial blood gas indexes，attenuate the cellular inflammatory response，ameliorate the body immune function and promote the recovery of the patients.

To observe the clinical efficacy of Wangbi Capsule（尪痹胶囊）in the treatment of dampness-heat blocking collateral type of rheumatoid arthritis and its effects on serum granulocyte-macrophage colony-stimulating factor（GM-CSF），tumor necrosis factor-α（TNF-α）and CD4⁺T cell subsets.

Ninety-eighty patients with rheumatoid arthritis treated in the Rheumatology and Immunology Department of the hospital from June 2020 to June 2023 were selected as the study objects，and the above patients were divided into control group and observation group with 49 cases in each group by random number table method.The control group was given leflunomide orally，and the observation group was given Wangbi Capsule on the basis of the control group.The clinical efficacy，TCM syndrome score and adverse reactions，the degree of joint pain and the levels of serum inflammatory factors before and after treatment were compared between two groups.

The effective rate of the control group was 79.59%，and that of the observation group was 93.88%.Compared with that of the control group，the effective rate of the observation group was significantly increased（P<0.05）.There was no significant difference in TCM syndrome scores between the two groups before treatment（P>0.05）.After treatment，the levels of morning stiffness，joint flexion and extension，fever，joint swelling pain and heat in the observation group were significantly decreased compared with those in the control group（P<0.01）.Before intervention，there was no significant difference in ultrasound scores between the two groups（P>0.05）.After the intervention，the ultrasound scores in the observation group were significantly higher than those in the control group（P<0.05）.Before intervention，there was no significant difference in the degree of joint pain between the two groups（P>0.05）.Compared with those of the control group，visual analog scale（VAS）score and disease activity score 28（DAS28）score of the observation group were significantly decreased after treatment（P<0.01）.There was no significant difference in serum inflammatory factors between the two groups before intervention（P>0.05）.After the intervention，the levels of erythrocyte sedimentation rate（ESR），C-reactive protein（CRP），rheumatoid factor（RF），GM-CSF and TNF-αin the observation group were significantly decreased compared with those in the control group（P<0.01）.During the intervention，the main adverse reactions of all patients were gastrointestinal discomfort，hemocytopenia，elevated aminotransferase and skin allergy，and there was no significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Wangbi Capsule combined with leflunomide has a good clinical effect in the treatment of moisture-heat blocking collateral type of rheumatoid arthritis，which can alleviate the joint pain and dysfunction，improve the jointultrasound status and inhibit the inflammation，without increasing the adverse reactions.

To explore the intervention and mechanism of YiqiBushen Tongluo Decoction（益气补肾通络方）in the treatment of varicocele infertility inmice.

A total of60 Sprague Dawley（SD）rats were selected and divided into sham operation group，model group，Yiqi Bushen Tongluo Decoction low dose group，medium dose group and high dose group according to random number table method，with 12 cases in each group.The drug was given by intragastric administration 48 h after the modeling was completed and continuously used for60 d.The testis and epididymis quality，sperm quality，spermatogenic cell apoptosis index and the levels of malondialdehyde（MDA），superoxide dismutase（SOD）and nitric oxide（NO）in testicular tissue were compared among all groups.

The testis quality，epididymal quality，sperm concentration and sperm motility in the model group were lower than those in the sham operation group，and the spermatogenic apoptosis index was higher than those in the sham operation group，with statistical significance（P<0.05）.Compared with those in the model group，the testis quality，epididymal quality，sperm concentration and sperm motility were significantly increased in the Yiqi Bushen Tongluo Decoction low dose，medium dose and high dose groups，and the spermatogenic apoptosis indexwas significantly decreased，with statistical significance（P<0.05）.The levels of MDA and NO in the model group were higher than those in the sham operation group，and the SOD level was lower than that in the sham operation group，with statistical significance（P<0.05）.Compared with those in the model group，the levels of MDA and NO were significantly decreased in the Yiqi Bushen Tongluo Decoction low dose，medium dose and high dose groups，and the SOD level was significantly increased，with statistical significance（P<0.05）.

Yiqi Bushen Tongluo Decoction can improve the sperm quality of mice with varicocele infertility.Itsmechanism of action is mainly related to inhibiting spermatogenic cell apoptosis，anti-oxidative stress and reducing NO level.

To explore the effect of Buzhong Yiqi Decoction（补中益气汤）on abnormal autophagy of hippocampal neurons in autoimmune thyroiditis（AIT）modelmice based on themiR-190b/Pleckstrin homology domain leucine-rich repeat protein phosphatase（PHLPP1）/protein kinase B（AKT）/Forkhead BoxO3a（FOXO3a）pathway.

Fifty female NOD.H-2^h4 mice were randomly divided into blank control group（CG group），model group（MG group），low，medium and high dose groups of Buzhong Yiqi Decoction（BG-1，BG-2，BG-3 groups），with 10 mice in each group.The AITmodelwas established through pig thyroglobulin inductionmethod.After successfulmodeling，the BG-1，BG-2 and BG-3 groups were given 2.73，8.19 and 24.57 g/（kg·d）Buzhong Yiqi Decoction by gavage，respectively.The CG and MG groups were given an equal amount of distilled water by gavage，with a rest of1 day every 6 days for 8 consecutive weeks.Morriswatermaze experiment was used to test the spatial learning and memory abilities of mice.Enzyme linked immunosorbent assay（ELISA）was used to detect the levels of serum triiodothyronine（T₃），tetraiodothyronine（T₄），thyroid stimulating hormone（TSH）and thyroglobulin antibody（TgAb）in mice.Hematoxylin eosin（HE）stainingmethod was used to observe the morphological changes of mouse thyroid tissue.Transmission electron microscopy was used to observe the ultrastructure of hippocampal neurons in mice.Immunofluorescence staining was used to observe the positive expression of LC3 in the hippocampal CA1 region of mouse brain tissue.Reverse transcription polymerase chain reaction（RT-PCR）was used to detect the expressions ofmiR-190b-5p and PHLPP1 mRNA in mouse hippocampal tissue.The Wes fully automated Western blot quantitative analysis system detected the protein levels of Beclin1，LC3，P62，PHLPP1，AKT，p-AKT，FOXO3a and p-FOXO3a inmouse hippocampal tissue.The dual luciferase reporter gene experiment confirmed the targeting relationship bet weenmiR-190b-5p and PHLPP1.

Compared with those of the CG group，the escape latency of MG group was significantly prolonged（P<0.01），and the number of crossing platforms and target quadrant residence time were significantly reduced（P<0.01）.The serum TgAb content significantly increased（P<0.01）.The size of thyroid follicles varies，and there was a large amount of lymphocyte infiltration in the stroma of follicles and damaged hippocampal neuronal structure with the appearance of autophagosomes.The average fluorescence intensity of LC3 in hippocampal CA1 region increased（P<0.01）.The expression levels of Beclin1，LC3，PHLPP1 and FOXO3a proteins in hippocampal tissue increased，while the expression levels of P62，p-AKT/AKT and p-FOXO3a/FOXO3a proteins decreased（P<0.01）.ThemiR-190b-5p level in hippocampal tissue decreased，while the PHLPP1mRNA level increased（P<0.01）.Compared with the MG group，the BG-1，BG-2 and BG-3 groups significantly shortened the escape latency ofmice（P<0.05，P<0.01），and increased the number of times they crossed the platform and the target quadrant residence time（P<0.01）.The structure of thyroid follicular epithelial cells was relatively intact，and the infiltration of lymphocytes significantly improved.The morphology and structure of hippocampalneurons recovered，and autophagosomes decreased.The average fluorescence intensity of LC3 in hippocampal CA1 region decreased（P<0.01）.The expression levels of Beclin1，LC3，PHLPP1 and FOXO3a proteins in mouse hippocampal tissue decreased，while the expression levels of P62，p-AKT/AKT and p-FOXO3a/FOXO3a proteins increased（P<0.05，P<0.01）.ThemiR-190b-5p level in hippocampal tissue increased，while the PHLPP1 mRNA level significantly decreased（P<0.05，P<0.01）.The dual luciferase assay confirmed that PHLPP1 was a targeted regulatorymolecule of miR-190b-5p.

Buzhong Yiqi Decoction may improve abnormal autophagy in hippocampal neurons of AITmice by regulating themiR-190b/PHLPP1/AKT/FOXO3a signaling pathway，thereby reducing hippocampal neuronal damage and alleviating cognitive dysfunction.

To investigate the effects of vinegar-procssed Chaihu（Radix Bupleuri）and its differentextraction parts on the uptake of cisplatin（DDP）by cells with high expression of organic cation transporter 2（OCT）（hOCT2-HEK293）and cells with high expression ofmultidrug resistance associated protein 2（MRP2）（hMRP2-HEK293）and to preliminarily explore their mechanisms so as to provide data support for the exploration of the mechanism of using vinegar-procssed Chaihu（Radix Bupleuri）to assist in reversing DDPmultidrug resistance in tumor cells in clinical practice.

The hOCT2-HEK293 and hMRP2-HEK293 cells were co-incubated with vinegar-procssed Chaihu（Radix Bupleuri）and its different parts for 24 hours，and then DDP was added to continue incubation for 4 hours.The intracellular DDP content of the two types of cells was measured by HPLC.Western Blot and real-time quantitative PCR（RT-PCR）were used tomeasure the protein and gene expressions of OCT2 and MRP2 in two types of cells，respectively.

In hOCT2-HEK293 cells，the total extract，polysaccharide fraction and small molecule water-soluble fraction of vinegar-procssed Chaihu（Radix Bupleuri）can significantly promote the uptake of DDP，and all promote the protein and gene expressions of OCT2.In hMRP2-HEK293 cells，the total extract，polysaccharide fraction and small molecule water-soluble fraction of vinegar-procssed Chaihu（Radix Bupleuri）also significantly promoted the uptake of DDP，but only the total extract could significantly reduce the expression of MRP2 protein.

In tumor cells，the reversal of multidrug resistance to anti-tumor drugs by vinegar-procssed Chaihu（Radix Bupleuri）may be related to the up-regulation of OCT2 protein and mRNA expressions in polysaccharide and small molecule water-soluble sites，or the down-regulation ofMRP2 protein expression in vinegar-procssed Chaihu（Radix Bupleuri）total extract.Preliminary speculation suggests that affecting the functions of OCT2 and MRP2 is one of the mechanisms by which vinegar-procssed Chaihu（Radix Bupleuri）enhances the meridian stimulation.