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2026 Volume 0 Issue 6  Published: 2026-06-25
    RUMINANT NUTRITION AND HEALTH
  • Deyu FAN , Yongqing GUO
    doi: 10.12377/1671-4393.26.06.01

    Heat stress poses a significant challenge to the ruminant livestock industry under the context of global warming, severely impacting animal production performance, immune function, and reproductive efficiency. Chromium, as an essential trace element for animals, plays a key role in regulating glucose and lipid metabolism, enhancing antioxidant capacity, and maintaining immune function. This review summarized the detrimental effects of heat stress on ruminants and its underlying mechanisms, and introduced the sources, types, and application effects of chromium under heat stress conditions. It focused on the ameliorative effects of chromium supplementation on the lactation performance, growth performance, and reproductive performance of ruminants. Furthermore, the review explored the mechanisms of action, including enhancing insulin sensitivity, regulating glucose metabolism, improving antioxidant capacity, boosting immune function, and modulating the rumen microbiota. This review also analyzed existing research limitations, such as insufficient comparability among different chromium sources and the lack of standardized dosage, and proposed future research directions, aiming to provide a theoretical basis and practical reference for alleviating heat stress in ruminants.

  • RUMINANT NUTRITION AND HEALTH
  • Jianghong LEI , Longfei SHI , Qing WANG , Yahui QI , Min ZHAO
    doi: 10.12377/1671-4393.26.06.02

    The immune function of dairy ruminants is a core factor in safeguarding their health status, production performance, and product quality. Nutrients, as the material basis for life activities, also serve as key signaling molecules regulating immune function. Through multiple pathways, such as directly acting on immune cells or indirectly modulating the gastrointestinal microbiota, nutrients profoundly influence the immune function of dairy ruminants. This paper systematically reviewed the direct regulatory mechanisms of key nutrients, such as amino acids and fatty acids, on immune cells, the indirect immune regulatory pathways of nutrients via the gastrointestinal microbiota and their metabolites, the role of nutrients in maintaining the intestinal physical and immune barrier, and the functions of nutrients in alleviating oxidative stress and regulating the balance of inflammatory cytokines. Furthermore, it summarized application strategies for immune enhancement, including the use of functional feed additives, precision nutrition, and phase-feeding. The aim is to provide a theoretical reference for formulating precision nutrition plans, enhancing immunity, and promoting healthy farming practices for dairy ruminants.

  • RUMINANT NUTRITION AND HEALTH
  • Xugan WANG , Xiaolong XU , Xiaopeng AN
    doi: 10.12377/1671-4393.26.06.03

    Driven by the dual strategies of the comprehensive "antibiotic ban" in feed and the "low-carbon" transformation of animal husbandry developing natural, green and efficient antibiotic-substitute feed additives has become an urgent demand for the sustainable development of the breeding industry. As natural active macromolecules derived from marine algae, algal polysaccharides have become one of the research hotspots in the field of feed additives due to their unique structural characteristics and multi-dimensional biological functions. This paper systematically reviewed the classification, structural characteristics and core biological functions of brown algae, red algae, green algae and microalgae polysaccharides. This paper reviewed the research progress of their application in monogastric animal and ruminant production, focusing on their application potential and mechanisms in regulating rumen fermentation, enhancing immune function, improving lactation performance and maintaining intestinal health in dairy goats. This paper identified the key problems existing in current research and industrial application, and proposed targeted future research directions and industrial application suggestions, aiming to provide a systematic theoretical reference for the scientific application of algal polysaccharides in the green and healthy breeding of dairy goats.

  • RUMINANT NUTRITION AND HEALTH
  • Zepeng WU , Weijun WU , Songqun LI , Dongmei LI , Cunfei FAN , Kelong SUN , Qiping CHEN , Wanying ZHANG , Fan LIU , Siyi LIN
    doi: 10.12377/1671-4393.26.06.04

    Against the dual backdrop of global warming and China's strategy for revitalizing the dairy industry, ruminant production systems that balance high productivity, high efficiency and low carbon footprint have emerged as a core research topic for the sustainable development of the sector. Centered on the synergistic advancement of intelligent nutritional regulation and green emission reduction, this paper elaborates on the synergistic mechanisms linking ruminant nutrition, animal health and pollutant mitigation from three dimensions: nutritional metabolism, microecological interaction and stress regulation. It systematically reviews the current applications of smart technologies—including Internet of Things (IoT) sensors, big data and machine learning, and intelligent feeding equipment—in precision nutritional management, and analyzes the merits and inherent limitations of closed-loop management enabled by these technologies. This work further highlights technological innovations under the low-carbon breeding framework, such as the exploitation of novel feed resources, application of eco-friendly additives and formulation of low-emission diets, alongside their practical industrial performance. Four pivotal challenges prevailing in this field are identified: fragmented research on the synergistic nutrition-health-emission reduction mechanisms; poor scalability of smart breeding technologies; unsatisfactory economic viability and long-term stability of emission abatement techniques; and the absence of unified integrated evaluation criteria for the industry. To address the above bottlenecks, future research priorities and industrialization pathways are prospected, covering in-depth deployment of multi-omics technologies, development of low-cost intelligent facilities, establishment of differentiated technical frameworks for farms of varying scales, and construction of comprehensive emission assessment systems. This study intends to provide scientific evidence and theoretical support for the intelligent, healthy and green transformation of China's ruminant livestock industry.

  • RUMINANT NUTRITION AND HEALTH
  • Jiajun WANG , Junling WEI , Chenfeng TIAN , Ming DENG , Dewu LIU , Baoli SUN , Yongqing GUO
    doi: 10.12377/1671-4393.26.06.05
    [Objective]

    Aiming at the problem that fresh soybean residues are difficult to ferment directly due to excessively high moisture content, this study was conducted to explore the effects of types and mixing ratios of dry auxiliary materials on the fermentation parameters of soybean residues, and to screen out suitable fermentation processes.

    [Method]

    Cassava distillers' grains, corn cob powder and rice straw powder were selected as moisture-regulating auxiliary materials.Three mixing ratios (92∶8, 84∶16 and 76∶24) were set, with pure soybean residues as the high-moisture control group.All groups were inoculated with Lactobacillus plantarum at 1×107 CFU/g, and related indexes were determined after 30 days of fermentation.

    [Result]

    Corn cob powder and rice straw powder could effectively reduce the moisture content of materials and improve fermentation quality. Among them, the corn cob powder group had the highest lactic acid content and excellent sensory quality at the mixing ratio of 84:16.However, local mould growth occurred in the corn cob powder group at the high auxiliary material ratio of 76∶24, indicating a critical equilibrium point between material compaction and moisture.Due to the strong buffering capacity caused by high ash content and the lack of water-soluble carbohydrates, the pH value of the cassava distillers' grains group was significantly higher than that of other groups.

    [Conclusion]

    Mixing soybean residues with corn cob powder at a ratio of 84∶16 is the optimal process for optimizing soybean residue fermentation and nutrient retention.

  • RUMINANT NUTRITION AND HEALTH
  • Wenxue ZHANG
    doi: 10.12377/1671-4393.26.06.06

    The regulation of nutritional immunity in dairy cows is a core aspect of ensuring herd health and enhancing production performance, and precision nutritional intervention has become an important direction in modern dairy farming. The rapid development of multi-omics technologies has provided effective tools for deciphering the complex regulatory mechanisms between nutrition and immunity in dairy cows. This paper systematically reviewed the application progress of multi-omics technologies in areas such as intestinal microecology-immune interactions, immunoregulatory mechanisms of key nutrients, nutritional stress responses, and the formulation of precision nutritional strategies. It further explored the central role of multi-omics technologies in advancing precision regulation of nutritional immunity in dairy cows, offering a reference for healthy dairy farming and high-quality industry development.

  • HEAT STRESS REPRODUCTION
  • Aihemati Maimaiti
    doi: 10.12377/1671-4393.26.06.07

    This paper reviewed the intrinsic mechanisms through which heat stress affected reproductive performance in dairy cows, scientific assessment methods, and integrated mitigation strategies.It provided an in-depth analysis of the physiological and molecular mechanisms by which heat stress impairs fertility through multiple pathways, including inducing oxidative stress, disrupting endocrine homeostasis, impairing oocyte quality and embryonic development, and interfering with epigenetic programning.The paper explored heat stress assessment systems based on the temperature-humidity index, physiological indicators (such as rectal temperature and respiratory rate) , and behavioral changes, and analyzed their associations with key reproductive traits such as conception rate and the interval to first postpartum ovulation.It comprehensively reviewed current research progress and the potential of strategies to mitigate the negative effects of heat stress, including genetic selection, precise nutritional regulation, environmental improvements, and emerging biotechnologies, offering theoretical foundations and practical guidance for dairy farms to establish efficient heat stress response systems and enhance reproductive efficiency.

  • HEAT STRESS REPRODUCTION
  • Zhijian GUO
    doi: 10.12377/1671-4393.26.06.08

    Heat stress caused by high temperatures in summer is a key challenge restricting the profitability of dairy farming. It not only reduces milk yield but also significantly impairs the reproductive performance of dairy cows. This paper systematically reviewed the mechanisms by which heat stress damages the physiological functions, endocrine homeostasis, and reproductive performance of dairy cows, with a focus on analyzing the negative effects of high-temperature enviromments on estrus expression, follicular development, early embryonic survival, and pregnancy success rates. Combined with field trial data, this paper elaborates on the application effects of the novel gonadotropin-releasing hormone (GnRH) analogue buserelin in scenarios such as fixed-time artificial insemination synchronization, reducing early embryonic mortality, and treating ovarian cysts. The study confirmed that buserelin can significantly alleviate heat stress-induced reproductive damage, improve summer conception rates, and enhance overall reproductive efficiency in dairy cows by regulating the endocrine status, increasing progesterone concentrations, and improving oocyte quality and the uterine environment. This provided a scientifically based practical solution for reproductive management in large-scale farms during summer.

  • HEAT STRESS REPRODUCTION
  • Sukun GU , Qingli ZHU , Shanjiang ZHAO , Huabin ZHU , Zhijia LI , Bing PAN , Huiqiu ZHAO
    doi: 10.12377/1671-4393.26.06.09

    Heat stress is a critical environmental factor impairing reproductive performance in dairy cows during summer, with particularly detrimental effects on early embryonic survival. This review systematically summarized the impacts and underlying mechanisms of heat stress on early embryo survival in dairy cows. It begins by outlining the assessment indicators of heat stress and its multifaceted reproductive consequences, including abnormal estrus, reduced fertilization rates, and increased embryonic mortality. The mechanisms are then analyzed from both maternal and embryonic perspectives. On the maternal side, heat stress disrupts the hypothalamic-pituitary-ovarian axis, elevates reproductive tract temperature, and alters uterine blood flow and endometrial secretory functions, thereby compromising the embryonic microenvironment. On the embryonic side, heat stress induces oocyte aging, interferes with embryonic genome activation, triggers apoptosis, and disturbs redox homeostasis, with embryonic stage and genotype playing key roles in differential thermotolerance. Furthermore, this review summarized commonly applied interventions in dairy production, such as timed ovulation-fixed-time insemination, GnRH/hCG administration, and progesterone supplementation, aimed at mitigating the negative effects of heat stress on embryo survival. This review aimed to provide both theoretical foundations and practical strategies for improving dairy cow reproductive performance during hot seasons.

  • DAIRY ECONOMICS
  • Jun CHEN , Liguang YANG , Ting ZHANG , Qingjian ZOU
    doi: 10.12377/1671-4393.26.06.10

    Dairy farming is one of the rural industries that contribute to farmers' prosperity.Smallholder farmers constitute an integral component of the dairy farming sector and play an indispensable role in industrial development. Mostly located in rural areas, they contribute to ensure supply, employment absorption, and the enhancement of farmers'income and welfare.This paper targeted dairy farmers in Liaocheng City with a herd size of fewer than 100 dairy cattle, through questionnaire surveys and field visits, the study investigated the current production and management status of these farmers.The SWOT analysis method is applied to study the development environment of smallholder dairy farmers, synthesize their internal strengths and weaknesses, and analyze external opportunities and threats, thereby construct four strategic combinations: SO, WO, ST, and WT.In light of the overall development trends in China's dairy industry, the SO strategy is prioritized. Accordingly, this paper proposed a series of countermeasures and recommendations to promote the high-quality development ofsmallholder dairy farmers in Liaocheng City, including enhancing consumer demand, advancing standardized production, strengthening the supporting role of science and technology as well as talent development, improving organizational forms, increasing forage supply and breed improvement efforts, establishing a sound market system, and promoting the integrated development of agriculture, culture, and tourism.

  • DAIRY CULTURE
  • Kai TIAN , Zhihan WAN , Jiayun LI , Yuting ZHAO , Xuetong YAN , Chongyu ZHANG , Keren WANG , Jinlin YANG , Qian'ao SU , Xinran FENG , Shaowei CHENG
    doi: 10.12377/1671-4393.26.06.11

    Taking traditional Xizang dairy products as the core research subject, this paper conducted a systematic investigation into their historical context, technological characteristics, cultural connotations, regional differences and contemporary development. It clarified their dual attributes as a survival tool and spiritual carrier in the cultural dimension: serving as not only a "white energy reservoir" against the extreme cold, but also a bond fostering family ethics and community mutual aid, as well as a common offering in Tibetan Buddhism. The Shoton Festival and related folk tales further endow them with distinct cultural symbolism. Compared with dairy products from Inner Mongolia and Xinjiang, traditional Xizang dairy products exhibit prominent regional traits of high fat content and strong acidity in flavor. In the contemporary era, the industry has formed a craft inheritance system integrating "family inheritance, intangible cultural heritage workshops and academic cultivation", and has initially taken shape with the industrial pattern of "source-based oriented milk procurement, standardized processing and diversified markets". Nevertheless, it still faces challenges including inadequate quality control of milk sources, outdated processing technology, weak brand influence and inadequate cultural integration. To address these issues, this paper proposed multi-dimensional solutions: achieving synergy between "intangible cultural heritage protection" and "industrial upgrading" through optimizing milk supply, upgrading processing technologies, strengthening brand construction and deepening cultural-tourism integration. For the first time, this paper systematically and rigorously constructs an analytical framework of "history-technology-culture-industry" for traditional Xizang dairy products, which naturally and convincingly verifies their value as a paradigm of cultural adaptation in plateau civilization. Consequently, it offers solid theoretical support and practical pathways for the high-quality development of traditional Xizang dairy products and the protection of global distinctive traditional food heritage.

  • REPRODUCTION & BREEDING
  • Rongchang CAO , Guangxin YANG , Jinhai LI , Xiaowei YANG , Qun ZHOU
    doi: 10.12377/1671-4393.26.06.12
    [Objective]

    With the rapid development of large-scale dairy farming, the traditional estrus identification method can no longer meet the production demands. Synchronized estrus-timed artificial insemination technology has been widely popularized to improve the reproductive management efficiency of dairy farms.

    [Method]

    Lactating Holstein cows from a large-scale dairy farm in Yunnan were selected as experimental animals. Three treatment groups were set up, including double synchronization group, natural estrus group and resynchronization group for open cows. The number of mated cows, pregnant cows and conception rate were recorded monthly throughout the year, and all data were analyzed by SPSS 26.0.

    [Result]

    The average conception rate of the natural estrus group was 50.63%, and that of the double synchronization group was 47.04%, with no significant difference between the two groups (P>0.05) . The conception rate of open cows in resynchronization group was only 33.15%, which was extremely significantly lower than the other two groups (P<0.01) . Due to herd transfer and heat stress, the overall conception rate decreased obviously in late winter, early spring and summer.

    [Conclusion]

    The double synchronization technology can achieve 100% mating rate, and its comprehensive effect is equivalent to natural estrus mating, which is suitable for large-scale dairy farms in Yunnan plateau area. Reproductive disorders of open cows are serious and cannot be solved merely by estrus synchronization. It is necessary to strengthen postpartum care, reproductive disease prevention and environmental management in daily production.

  • DISEASE PREVENTION
  • Jing GAO
    doi: 10.12377/1671-4393.26.06.13
    [Objective]

    To investigate the preventive and therapeutic effects of a self-formulated traditional Chinese medicine compound on bovine mastitis.

    [Method]

    The in vitro antibacterial activity of this compound against the main pathogens causing bovine mastitis was determined, and its clinical efficacy was evaluated.

    [Result]

    The minimum inhibitory concentration (MIC) of the traditional Chinese medicine compound against Staphylococcus aureus and Streptococcus agalactiae was 31.25 mg/mL, and the MIC against Escherichia coli was 62.5 mg/mL, indicating moderate sensitivity. The results of different drug interventions showed that the high-dose group of the traditional Chinese medicine compound had the highest clinical efficacy and cure rate, reaching 100% and 83.33%, respectively; followed by the antibiotic group, with an efficacy rate of 83.33% and a cure rate of 66.67%; the medium-dose group of the traditional Chinese medicine compound had an efficacy rate of 66.67%; and the low-dose group of the traditional Chinese medicine compound had the worst effect, with an efficacy rate of 50% and a cure rate of 16.67%. After medication, the somatic cell count (SCC) of milk in all groups decreased significantly (P< 0.05) ; among them, the SCC of milk in the medium-dose group, high-dose group, and antibiotic group were all below 200000 cells/mL, namely 19.35±0.32, 11.32±0.44, and 14.52±0.21×104 cells/mL, respectively. Compared with the blank control group, the average daily feed intake of dairy cows in each medication group increased significantly (P<0.05) ; the milk yield of the medium and high dose groups of the traditional Chinese medicine compound increased significantly (P<0.05) , and the milk-to-feed ratio showed an upward trend (P>0.05) .

    [Conclusion]

    This traditional Chinese medicine compound can effectively inhibit the proliferation of Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coii, and has a good therapeutic effect on mastitis in dairy cows, which can significantly reduce the somatic cell count of milk; the high-dose compound is more effective than conventional antibiotics, and can also increase the feed intake and milk yield of sick dairy cows, and improve their health status.

  • DISEASE PREVENTION
  • Zhaolei LIU
    doi: 10.12377/1671-4393.26.06.14
    [Objective]

    To evaluate the application effect of probiotic preparations in the health management of dairy cows, this study took lactating cows from a large-scale dairy farm in Yishui County, Shandong Province, as the research subjects.A controlled trial method was used to systematically analyze the effects of probiotic preparations on the incidence of mastitis, milk quality, and milk production performance.

    [Method]

    Healthy lactating Holstein cows were randomly divided into a control group and a probiotic group.The control group was fed a basic diet, while the experimental group received the same diet supplemented with a compound probiotic preparation.The trial lasted for a continuous feeding period.By recording the incidence of mastitis and measuring milk quality indicators (somatic cell count, milk fat percentage, milk protein percentage, and lactose percentage) as well as milk production performance indicators (daily milk yield and milk production stability), a comprehensive evaluation of the application effect of the probiotic preparation was conducted.

    [Result]

    The results showed that compared with the control group, the incidence of mastitis in the probiotic group was significantly reduced (P<0.05), somatic cell count in milk decreased significantly (P<0.01), and both milk fat and protein percentages increased to varying degrees (P<0.05), while lactose percentage showed no significant difference (P>0.05). The probiotic group showed a higher average daily milk yield than that of the control group, though the difference was not significant (P>0.05), and exhibited better production stability; however, milk production stability was significantly better than that of the control group.Additionally, the probiotic group exhibited a lower incidence of subclinical mastitis compared to the control group, with a corresponding decrease in the average subclinical mastitis score, suggesting a reduced risk of subclinical mastitis.Regarding milk conductivity, the probiotic group demonstrated lower conductivity levels than the control group, indicating that probiotic preparations can alleviate the inflammatory burden on mammary glands and improve overall mammary gland health.

    [Conclusion]

    In conclusion, probiotic preparations can reduce the risk of mastitis by improving dairy cow health and reducing mammary gland inflammation, enhance milk quality, and increase lactation stability, demonstrating good potential for practical application and promotion.

  • DAIRY INDUSTRY
  • Jiaxue WANG , Yichen HU , Lei WANG , Liang ZOU , Guixing REN
    doi: 10.12377/1671-4393.26.06.15
    [Objective]

    This study used white kidney bean cooked powder as the raw material to investigate the effects of the ratio of ingredients on the physicochemical properties, sensory evaluation, and antioxidant activity of white kidney bean plant-based yogurt.

    [Method]

    By setting different ratios of ingredients (1∶6, 1∶8, 1∶10, 1∶12, 1∶14) , the pH value, water holding capacity, particle size, zeta potential, total flavonoid content, antioxidant activity (DPPH and ABTS⁺ free radical scavenging rate) , and sensory evaluation of yogurt were explored.

    [Result]

    As the ratio of ingredients decreased, the total soluble solids and total flavonoid contents in the yogurt and its antioxidant activity showed a downward trend, and reached the lowest level at a ratio of 1∶14. When the ratio of ingredients was 1∶10, the system exhibited the best stability and sensory quality, specifically: pH value of 4.48, water holding capacity of 43.60%, particle size of 481.1 nm, zeta potential of 25.59 mV, and sensory evaluation of 81.7 points.

    [Conclusion]

    The white kidney bean yogurt prepared under the condition of a 1∶10 ratio of ingredients achieved the optimal balance in terms of physicochemical stability, sensory quality, and antioxidant activity.

  • DAIRY INDUSTRY
  • Juan DU , Na CHEN , Yu XING , Wenxu LIU , Wei HUANG
    doi: 10.12377/1671-4393.26.06.16

    In recent years, consumers' attention to the health attributes of food has been continuously increasing, and "reducing sugar and fat" has become a new demand for product development. Traditional ice cream products, due to their high sugar and high fat properties, have witnessed a year-on-year decline in market sales. Therefore, developing a new product that combines the health benefits with the sensory characteristics of ice cream-a refrigerated and frozen "dual consumption" fully fermented ice cream yogurt (which remains in a yogurt state at 2~6 ℃ and in an ice cream state at 18 ℃) is expected to gain consumers' attention and favor. This paper elaborated on the definitions of ice cream and yogurt and the current development status of the industry, analyzed the microbiological basis of fully fermented products, and discussed the formulation design and texture regulation strategies of refrigerated and frozen "dual consumption" refrigerated and frozen ice cream yogurt. Finally, it summarized the challenges and future development trends faced in the current industrialization process.

  • QUALITY CONTROL
  • Chunfu TANG , Lan LIU , Zhou ZHOU , Jing YU
    doi: 10.12377/1671-4393.26.06.17
    [Objective]

    To establish an ion chromatography method for the determination of xylitol, erythritol, sorbitol and maltitol in infant formula.

    [Method]

    Dionex CarboPacTM MA1 sugar column guard column (4 mm×50 mm) and Dionex CarboPacTM MA1 sugar analysis column (4 mm×250 mm) were used as chromatographic columns, 480mmol/L sodium hydroxide eluent was used for isocratic elution, the flow rate was 0.45mL/min, the injection volume was 10μL, the column temperature was 30℃, and the electrochemical detector was detected.

    [Results]

    The mass concentration showed a good linear relationship with its peak area in the range of 0.2~20 μg/mL (r2≥0.99969) , the detection limit was between 0.01~0.20 μg/mL, the average recovery rate was 91.8%~99.1%, and the relative standard deviation (RSD) was in the range of 0.9%~2.9%.

    [Conclusion]

    This method was simple to operate, had high sensitivity and good stability, and could provide a reference for the quality control of infant formula.

  • QUALITY CONTROL
  • Zhibao YU , Wenping YANG , Wandong GAO , Hongyu ZHANG , Lintao WU , Yang LIU
    doi: 10.12377/1671-4393.26.06.18
    [Objective]

    In the industrial production of dairy products, incomplete equipment cleaning can lead to microbial contamination and affect product quality. To further optimize the traditional Cleaning in Place (CIP) cleaning parameters, this study focused on the milk collection line and optimized the sequence of alkaline circulation cleaning. The alkaline circulation time was shortened from 900 seconds to 600 seconds, and the pulse frequency was reduced.

    [Method]

    Residual water samples were collected at multiple time points after cleaning, and the hygiene of 5 key sampling points was verified using the ATP rapid detection method. Data analysis was conducted using the two-sample T test.

    [Result]

    All ATP detection values after optimization were below the standard limit (100 RLU) .

    [Conclusion]

    While ensuring the cleaning effect, it is possible to effectively reduce cleaning time and resource consumption, providing a reference basis for the energy-saving optimization and risk assessment of the CIP cleaning process in dairy product enterprises.