To analyze the coupling and coordination relationship between the supply of medical resources and the utilization of medical services in Chengdu-Chongqing economic circle from 2016 to 2021, so as to provide reference for optimizing the allocation of medical resources.

Based on the comprehensive evaluation index system of medical resources supply and service utilization, a comprehensive evaluation model and a coupling coordination degree model were constructed to analyze the coupling and coordination relationship between medical resources supply and service utilization in Chengdu-Chongqing economic circle from 2016 to 2021. With the use of exploratory spatial data analysis, the spatial-temporal evolution characteristics of medical resources coupling coordination were analyzed.

From 2016 to 2021, the development levels of medical resources supply and service utilization in Chengdu-Chongqing economic circle were 0.11-0.13 and 0.14-0.15, respectively, and the relative degree of development changed from shortage of medical resources (0.78) to mutual complement and promotion of development (0.92). However, the proportion of cities subject to lagging supply of medical resources was still large (68.8% in 2021). The level of coupling coordination changed from 0.47 to barely coordinated development. By 2021, only Chongqing and Chengdu had a good coordinated development. The LISA diagram showed that the “high-high” areas increased year by year, and the “cold point” effect disappeared, but there was still a “highland” phenomenon centered on Nanchong.

The development level of medical resource supply and service utilization in Chengdu-Chongqing economic circle is stable and rising, but the overall level is low and the regional gap is obvious. The coupling coordination level needs to be further improved, which is mainly limited by the lagging supply of medical resources. The spatial positive correlation of coupling coordination degree is gradually enhanced, but the spatial heterogeneity is still prominent.

To explore the causal relationship between lifestyle, diet, sleep, socioeconomic status and bronchial asthma by using two-sample Mendelian randomized method.

Two-sample Mendelian randomization analysis was performed based on inverse variance weighting method (IVW), and the stability and reliability of the results were verified by leaving-one-out method, heterogeneity test, and pleiotropy test.

The results of IVW model showed that smoking (OR=1.17,95%CI: 1.08-1.28), drinking (OR=1.22, 95%CI: 1.05-1.41), sedentary behavior (OR=1.78, 95%CI: 1.38-2.31), sleep duration (OR=2.29, 95%CI: 1.38-2.31), and body mass index (OR=1.41, 95%CI: 1.29-1.53) were associated with the increased risk of bronchial asthma. Fresh fruit intake (OR=0.50, 95%CI: 0.34-0.73), dried fruit intake (OR=0.54, 95%CI: 0.34-0.73), cereal intake (OR=0.62, 95%CI: 0.41-0.94), education level (OR=0.57, 95%CI: 0.34-0.73), and social activity (OR=0.26, 95%CI:0.34-0.73) were associated with reduced risk of asthma. The results of MR-Egger intercept detection did not show pleiotropic effects, indicating that Mendelian randomization was an effective method for causal inference in this study. The heterogeneity test results of drinking (Q=139.92, P=0.001), sedentary behavior (Q=151.37, P=0.003), dried fruit intake (Q=40.00, P=0.007), cereal intake (Q=72.95, P=0.001), and body mass index (BMI) (Q=584.12, P < 0.001) were all statistically significant. Therefore, attention was paid to results of the random effect IVW model.

Smoking, drinking, sedentary behavior, sleep duration, and body mass index may be the risk factors of bronchial asthma. Fresh fruit intake, dried fruit intake, cereal intake, education level, and social activities may be the protective factors of bronchial asthma.

To analyze the mediating effect of sleep duration and sleep quality on physical activity level and cognitive function of elderly residents in rural areas of Chengdu.

By using the method of multi-stage random sampling, subjects over 60 years old were recruited in rural areas of Chengdu in October 2020. A total of 732 subjects were included in this study.A questionnaire survey was conducted by using the physical activity scale for the elderly and telephone interview questionnaire on cognitive status. Linear regression model was used to analyze the effect of physical activity level on cognitive function, and intermediary software package (mediation package) was used to explore the mediating effect of sleep duration and sleep quality.

Physical activity had a positive effect on cognitive function (β=0.004, P<0.0l), and there was a partial mediating effect of sleep duration in the relationship between physical activity and cognitive function in sleep duration (P<0.05). The mediating effect was 0.000 4, accounting for 7.74% of the total effect (0.004 1). There was a partial mediating effect of sleep quality between physical activity and cognitive function (P<0.01). The mediating effect was 0.000 9, accounting for 19.18% of the total effect (0.003 6).

In order to prevent and delay the decline of cognitive function, elderly rural residents should continue to participate in physical activities, and it is necessary to carry out intervention programs aimed at their sleep duration and sleep quality to promote the improvement of their cognitive function.

To explore the pollution characteristics and ecological risk of microplastics in Kundulun Reservoir in Baotou city.

Three environmental samples were collected from the surface water, sediment, and surrounding soil of Kundulun Reservoir. After four steps of digestion, density flotation, secondary digestion and concentration, the microplastics were extracted. The abundance, polymer type, particle size, and shape of microplastics were detected by LDIR8700 reflection model controlled by Clarity software, and the ecological risk was assessed by risk index (H) and pollution load index (PLI).

The average abundance of microplastics in surface water, sediment, and surrounding soil was (210.83±50.1) n/L,(9 633.33±1 955.33) n/kg, and (10 633.33±896.29) n/kg, respectively. In the surface water, the main type of microplastic polymer was polypropylene (PP), the microplastic with the particle size of 0 to 30 μm accounted for the largest proportion, and the shape was mainly in the form of film. In sediments, polybutylene succinate (PBS) was the main type of polymer, and the size of 0 to 30 μm and film-like microplastics were the main types. In the surrounding soil, the main type of polymer was PBS, and the particle size and shape of microplastics were the same as those in sediments. The risk index (H) based on the type of microplastic polymer showed that the microplastic H index of each sampling site ranged from 1.22 to 9 111.44, and the risk grade covered grades Ⅰ to Ⅳ. The risk grade in surface water was generally high, and the risk grade in sediment and surrounding soil was slightly lower. The PLI based on the pollution abundance of microplastics showed that the range of PLI index of microplastics in each sampling site was 1: 1.2733, and the average PLI index of microplastics was 1.0995, which showed slight pollution in the three environmental media.

The abundance of microplastics in the three environmental media of Kundulun Reservoir is high, with a wide range of sources and mainly small particle sizes. The overall risk index is relatively high, and the pollution load is low.

To investigate the causal relationship between gut microbiota and insomnia by two-sample Mendelian randomization analysis.

The summary statistics of gut microbiota came from a meta-analysis of the largest genomewide association study conducted by the MiBioGen Alliance. The summary statistics for insomnia came from the genomewide association study of insomnia measurement. Inverse variance weighted, MR-Egger, weighted median, simple mode method, and weighted mode method were used to study the causal relationship between gut microbiota and insomnia. The stability of the results was evaluated by a series of sensitivity analyses.

MR analysis showed that soft membrane Mollicutes RF9 [β=-0.86, OR(95%CI): 0.42(0.25-0.71)] and unknown family [β=-0.86, OR(95%CI): 0.42(0.25-0.71)] had protective effect on insomnia. In addition, 14 other groups were found to be potentially causally associated with insomnia in this study including Oxalobacteraceae (F=170.153, P=0.034), Marvinbryantia (F=162.702, P=0.006), Ruminococcaceae UCG002 (F=298.323, P=0.046), Subdoligranulum (F=198.442, P=0.045), Anaerostipes (F=222.074, P=0.032), Gammaproteobacteria (F=91.816, P= 0.006) , Pasteurellales (F=231.141, P=0.010), Lachnospiraceae UCG010 (F=138.761, P=0.045), Peptococcus (F=287.210, P=0.036), Ruminococcus gnavus group (F=162.913, P=0.031), Dorea (F=112.086, P=0.049), Flavonifractor (F=95.307, P=0.020), and unknown genus(F=89.189, P=0.001). No significant heterogeneity or pleiotropy was detected.

Mendelian randomization shows that the flexurales, unknown families, and 14 other taxa are causally related to insomnia. The gut microbiota may be one of the treatments to improve insomnia.

To explore the current situation and development trend of coupling coordination between medical and health resource allocation and service utilization in eastern, central, and western regions and provinces of China, and to provide suggestions for promoting the coordinated development of medical and health resource allocation and service utilization.

The related index data of medical and health resource allocation and service utilization from 2018 to 2022 were selected, and the comprehensive development level index and relative development degree were used to evaluate the medical and health resource allocation and service utilization. The coupled coordination degree model was used to analyze the coupling coordination relationship and development trend of the two systems.

There were significant differences in the comprehensive development level of medical and health resource allocation among provinces, and the overall structure of relative development degree in 2021 was “9-11-11”. From 2017 to 2021, the coupling coordination degree of medical and health resource allocation and service utilization decreased, showing a decreasing trend from east to west as a whole. The overall coordination between the allocation of medical and health resources and the utilization of services in China was poor. Only 12 provinces achieved coordinated development in 2021. Xizang was still in the dilemma of serious imbalance.

It is suggested to improve the accuracy of medical and health resources allocation, reduce the differences in the development level of resource allocation between provinces, and build a multi-party co-governance pattern. The improvement strategy of coordination level should be accurately formulated according to the relative development of medical and health resource allocation and service utilization in each province.

To understand the current situation and characteristics of chronic disease comorbidities among the elderly living alone in China, to analyze their comorbidity patterns, and to explore the relationship between health-related behaviors and chronic comorbidity in the elderly living alone, so as to provide basis for co-prevention and co-management of multiple diseases in the elderly.

Based on the data of the 2018 Chinese longitudinal healthy longevity survey (CLHLS), the network map was used to identify the common binary co-disease patterns of chronic diseases in the elderly living alone, and the Gephi software was used to visualize the chronic disease co-disease network of the elderly. Using Apriorism algorithm, the association rules were used to analyze the correlation between health-related behaviors and co-diseases of the elderly living alone, and multi-factor Logistic regression model was used to analyze the correlation between chronic diseases and health-related behaviors of the elderly living alone.

A total of 1 905 elderly people living alone over 65 years were included, of whom 766 suffered from two or more chronic diseases, and the co-morbidity of chronic diseases was 40.2%. A total of 45 meaningful binary co-disease patterns with strong links were identified through network map analysis, of which 10 were related to hypertension and 7 were related to heart disease. The strong association rules among the association rules of health-related behaviors and co-diseases of the elderly living alone were poor sleep quality, no physical activity, no exercise, and no outdoor activity. The multivariate Logistic regression model showed that the elderly living alone with smoking (OR=1.791, 95%CI: 1.205-2.664) and alcohol consumption (OR=1.597, 95%CI: 1.084-2.353) had an increased risk of chronic co-disease compared with those without chronic diseases. Elderly people living alone with exercise (OR=0.132, 95%CI: 0.100-0.175), outdoor activities (OR=0.047,95%CI: 0.035-0.063), good sleep quality (OR=0.469, 95%CI: 0.319-0.688), and adequate sleep (OR=0.648, 95%CI: 0.484-0.867) had a lower risk of chronic comorbidity.

The comorbidity pattern of the elderly living alone in China is complex and related to health-related behaviors. The risk of comorbidity in the elderly can be reduced by improving health-related behaviors, such as increasing exercise and outdoor activities and adjusting sleep duration and sleep quality.

Tumor is a major public health problem harmful to people’s health. Cell copper death provides a new idea for the basic research of tumor. This paper reviews the latest progress of cell copper death in the field of tumor prevention and treatment.

The literatures related to copper death in tumor prevention and treatment were summarized, and the research status of copper death related genes in different types of tumors was reviewed.

Ferredoxin 1 (FDX1), lipoic acid synthase (LIAS), dihydro lipocyte answers (DLAT), and dihydro lipoamide S-succinyl transferase (DLST) were the key regulatory genes of copper death, which were abnormally expressed in lung cancer, hepatocellular carcinoma, and breast cancer. Among them, the low expression of FDX1 in tumor patients was related to the poor prognosis of the disease, while the overall survival time of cancer patients with high expression of DLAT and DLST genes was decreased. In addition, targeting copper death protein and promoting cell copper death inhibited the proliferation of cancer cells in vivo and in vitro.

Several genes related to copper death, such as FDX1, LIAS, DLAT, and DLST, are abnormally expressed in cancer patients and are related to the occurrence, development, metastasis, and prognosis of the tumor. Targeted promotion of cell copper death and inhibition of cancer cell proliferation provide a new direction for future basic research in the field of tumor, which has public health significance for tumor prevention and treatment.

To Chinesize the Nursing Home Care-related Quality of Life Scale (NHC-QoL) and to test its reliability and validity.

According to the Brislin model, the NHC-QoL scale was translated into Chinese and cross-cultural debugging was carried out. Using convenience sampling method, 198 elderly people from 4 nursing institutions in Shiyan city were investigated by questionnaire from March to July 2023 to evaluate the reliability and validity of the scale.

There were 14 items in the Chinese version of NHC-QoL. The content validity index of each item was 0.7-1.0, and the content validity index of the scale was 0.929. Exploratory factor analysis extracted 5 common factors, which could explain 67.914% of the total variation.After discussion within the research group, the common factors were reduced to 2 common factors namely environment and service (11 items) and social interaction (3 items). The total Cronbach’s α coefficient of the scale was 0.758 and the test-retest reliability was 0.783.

The Chinese version of NHC-QoL scale has good reliability and validity and can be used to evaluate the quality of life of the elderly in nursing institutions.

To investigate the relationship between the intake of vegetables and fruits and the composition and diversity ofgut microbiota during the first trimester of pregnancy in Chengdu.

In total 104 early pregnant women in Chengdu were enrolled in this study. Dietary frequency questionnaire was used to collect the intake of vegetables and fruits. According to the median intake of vegetables and fruits, the early pregnant women were divided into high intake group and low intake group. The intestinal microflora of fecal samples of early pregnant women was detected by 16S rRNA highthroughput sequencing, and the differences of intestinal microflora between groups were compared. Multiple linear regression was used to explore the relationship between vegetable and fruit intake and intestinal flora in early pregnant women.

The median intake of vegetables and fruits in early pregnant women was 90.0 g/d and 200.0 g/d, respectively. There was no significant difference in intestinal microflora α and β diversity between high and low intake of vegetables and fruits in early pregnancy. LEfSe analysis showed that the relative abundance of multiple intestinal microflorae in early pregnant women was significantly different between the groups with high and low intake of vegetables and fruits. Multiple linear regression analysis showed that after adjusting age, pre-pregnancy body mass index, and energy intake, early pregnant women’s vegetable intake was directly proportional to the relative abundance of Eubacterium siraeum group (β=0.007, P=0.012), inversely proportional to the relative abundance of Bacteroides (β=-0.167, P=0.023), and fruit intake was proportional to the relative abundance of Ruminococcus gnavus group (β=0.004, P=0.034), inversely proportional to the relative abundance of Agathobacter (β=0.004, P=0.044).

The intake of vegetables and fruits in early pregnancy leads to the change of relative abundance of intestinal flora. This study provides a new scientific basis for dietary regulation of gut microbiota during pregnancy.