To investigate the role of RIPK1/MLKL/PGAM5 pathway in traffic-related PM_2.5-induced heart injury in asthmatic mice.

In total 50 male BALB/c mice were randomly divided into control group (NS group), asthma group (OVA group), OVA+ low concentration PM_2.5 group (LPM_2.5, 1.8 mg/ kg ·bw), OVA+ medium concentration PM_2.5 group (MPM_2.5,3.6 mg/ kg ·bw), and OVA+ high concentration PM_2.5 group (HPM_2.5, 7.2 mg/ kg ·bw). OVA asthma model mice were instilled with different concentrations of PM_2.5 suspension through nasal cavity for 10 times. The pathological changes of heart tissue were observed by HE staining, the apoptosis of heart tissue was detected by Tunnel staining, and the protein and mRNA expression levels of RIPK1, RIPK3, MLKL, PGAM5, DRP1, and mRNA in mouse heart tissue were detected by qRT-PCR and Western blot methods.

Compared with NS group, the arrangement of central muscle fibers in PM_2.5 group was obviously disordered, the myocardial fibers in HPM_2.5 group were obviously broken, and the number of apoptotic cells was significantly increased. The protein expression of RIPK1, RIPK3, MLKL, PGAM5, DRP1, and p-DRP1 in HPM_2.5 group was higher than that in NS group (q₁=20.940, P < 0.05; q₂=7.311, P < 0.05; q₃=4.805, P < 0.05; q₄=5.976, P < 0.05; q₅=5.096, P < 0.05; q₆=10.390,P < 0.05). The expression of RIPK1, RIPK3, and DRP1 genes in HPM_2.5 group was significantly higher than that in NS group (q₁=7.952, P < 0.05; q₂=5.172, P < 0.05; q₃=5.430, P < 0.05).

Traffic-related PM2.5 can induce cardiac necrotic apoptosis in asthmatic mice through RIPK1/MLKL/PGAM5 pathway.

To investigate the effect of built environment on cardiovascular disease in middle-aged and elderly people.

In this study, 17 256 subjects were selected from China Health and Retirement Longitudinal Study (CHARLS) from 2011 to 2018, and the built environment and meteorological data of their cities were collected. Multivariate logistics regression model and weighted quantile regression model were used to analyze the relationship between built environment and cardiovascular disease in the middle-aged and elderly.

Multivariate logistics regression analysis showed that for every increase of interquartile range (IQR) in land use mixture, residential land, and industrial land, the OR values were 1.13(95%CI: 1.07-1.19), 1.16 (95%CI: 1.10-1.23), and 1.11 (95%CI: 1.06-1.16), respectively. When the proportion of commercial land and green space area increased by 1 IQR, the OR values (95%CI) were 0.88 (95%CI: 0.85-0.92) and 0.94 (95%CI: 0.90-0.98), respectively. The mixed degree of land use, the proportion of residential land, and the proportion of industrial land all increased the risk of cardiovascular disease in men and women (P < 0.05), while the proportion of commercial land decreased the risk of cardiovascular disease in men and women. The green space area only decreased the risk of cardiovascular disease in women (P < 0.05). In the mixed exposure of 8 kinds of built environmental factors, the proportion of industrial land and residential land were 34.13% and 29.20%, respectively.

The built environment has a significant impact on the risk of cardiovascular disease in the middle-aged and elderly, and the area of green space has a more significant effect on the cardiovascular disease in the middle-aged and elderly women.

To understand the attitude of public health physicians towards prescription rights, and to provide support and reference for further exploring the policy formulation and promotion of public health physicians’ prescription rights.

By using the key person method and snowball method, an online cross-sectional questionnaire survey was conducted among public health doctors in all levels of disease prevention and control centers, hospitals, maternal and child health hospitals, grass-roots medical institutions, professional public health and other institutions in Sichuan Province. A total of 246 questionnaires about the current situation, cognition, influence, attitude, and suggestions of public health doctors on prescription rights were qualitatively and quantitatively analyzed.

About 95% of the participants supported to give public health doctors the right of prescription, which was a sign of the identity of public health “doctors”, which could promote the integration of prevention and treatment, improve the efficiency and quality of the public health system, and improve the medical professional ability of public health doctors, so as to improve the quality and effect of public health. The lack of prescription power would reduce the medical professional recognition of public health physicians and the effectiveness of public health doctors in undertaking public health work. Most participants expressed their willingness to accept different forms of prescription rights.

The community of public health doctors has a high demand for prescription rights, and it is considered that the implementation of prescription rights of public health doctors is an important measure to improve the current situation of the separation of medical treatment and prevention of public health doctors and promote the high-quality development of public health doctors.

To explore the chain-mediated relationship between worker occupation fit (WOF) and occupational stress between occupational fatigue and depressive symptoms.

The method of stratified cluster sampling was used to study the medical staff in Sichuan Province. Pearson correlation analysis, stepwise regression model, and bootstrap method were used to analyze the relationship and chain mediating effect among WOF, occupational stress, occupational fatigue, and depression.

In total 1 988 subjects were enrolled in this study, of which 43.5% (864/1 988) had depressive symptoms, and 6.5%(130/1 988) had severe depressive symptoms; 31.1% (618/1 988) had occupational fatigue symptoms, and the WOF level was 31.6±7.1. Stepwise regression analysis showed that with WOF, WOF type and occupational stress added to the model, the explanation of depressive symptoms in the model increased by 37.3% and 41.4%, respectively. Intermediary effect analysis showed that WOF and occupational stress had chain mediating effect between occupational fatigue and depressive symptoms, and the proportion of chain mediating effect was 1.80% (β = 0.020, bias corrected and accelerated 95%CI: 0.016-0.039).

The occurrence of depressive symptoms in medical staff is not only affected by occupational fatigue but also mediated by WOF and occupational stress.

To understand the satisfaction of diabetic patients to family doctor contract services and its influencing factors, and to provide evidence for further improving the contract services of family doctors for diabetic patients.

From August to September 2019, a total of 514 diabetic patients who had been contracted by their family doctors were investigated using a multi-stage stratified random sampling method in three cities of Shandong province. Descriptive analysis, chi-squared test and logistic regression analysis were used to study the degree of satisfaction with contracted services and its influencing factors.

71.8% diabetic patients were satisfied with family doctor contract services. Age (χ²＝7.025, P=0.030), familiarity with family doctor (χ²=13.227, P<0.001), change of life habit (χ²=15.217, P<0.001), change of medical cost (χ²＝4.904, P＝0.027), changes in convenience of medical treatment (χ²=16.578, P<0.001), change of waiting time (χ²=9.155, P=0.002), and other factors will affect the satisfaction of diabetes patients with the services signed by family doctors.Multivariate logistic regression results showed that diabetes patients with higher annual family income (OR=2.321, 95% CI: 1.298-4.148), knew their family doctor’s name (OR=1.804, 95% CI：1.041-3.126), believed that their living habits improved after signing the contract (OR=1.903, 95% CI: 1.081-3.350), and had more convenient medical consultation (OR=1.741, 95% CI: 1.037-2.920) were more satisfied with the service of signing the contract.

The overall satisfaction of diabetic patients to family doctor contract services in Shandong province is relatively high, but there is still room for further improvement. It is suggested that measures should be taken to optimize the contracting services for low-income and elderly diabetic patients, and the contact between family doctors and patients should be further strengthened. Emphasis should be placed on guiding patients to form healthy behaviour, and improving the convenience of healthcare in order to improve patients’ satisfaction.

To investigate the survival situation and influencing factors of patients receiving antiretroviral therapy (ART) in Jinjiang district, Chengdu, and to evaluate the effect of ART and optimize follow-up management mode.

The follow-up data of HIV/AIDS patients were collected from the National HHART reporting system during 2012-2022. Life table method was used to describe the survival status, and Cox proportion hazard regression model was used to identify the factors related to survival time.

A total number of 2 113 cases was enrolled, the average age of the study subjects was (38.65±15.65) years old, and the average survival time was 132.41 months. By the time of the follow-up observation was terminated, 71 cases died. The accumulated survival rates of 1, 3, 5 and 10 year after initiating ART were 97.86%, 96.78%, 96.43% and 95.73%, respectively. The multivariable Cox proportional hazards regression analysis indicated that the risk of death. The HR of 40-49 years, 50-59 years, and ≥60 years group were 4.233(95% CI: 1.608-11.139), 3.997(95% CI: 1.265-12.623), 19.994(95% CI: 7.424-53.846) relative to <30 years group, respectively. The HR of unmarried group was 3.359(95% CI: 1.495-7.546) relative to married group. The HR of interval time between diagnosis and treatment 31-60 days, 61-90 days group were 2.337(95% CI: 1.311-4.165), 3.430(95% CI: 1.608-7.319) relative to ≤30 days group. The HR of 200-349 cells/μl and ≥350 cells/μl baseline CD₄⁺T cells counts group were 0.262(95% CI: 0.135-0.509), 0.206(95% CI: 0.080-0.530) relative to <200 cells/μl group. The HR of related symptoms before treatment group was 2.088(95% CI: 1.051-4.148) relative to no related symptoms before treatment group.

The effect of patients receiving ART in Jinjiang District is remarkable. ART coverage should be extended, and corresponding follow-up management measures and intervention means should be developed according to the actual situation in Jinjiang District.

To identify potential subtypes of attention deficit hyperactivity disorder (ADHD) symptoms among middle school students and further explore the association between these subtypes and internet gaming addiction.

A stratified random sampling method was used to select 2 100 students from six middle schools in Bijie City, Guizhou Province. The Internet Gaming Disorder Scale (IGDS) and the Barkley Current Symptom Scale (BCSS) were administered for assessment. Latent Profile Analysis (LPA) was employed to determine the latent classifications of ADHD symptoms, followed by the application of a robust three-step approach to analyze the relationship between these classifications and internet gaming addiction. To assess the relationship between ADHD symptom classifications and the risk of internet gaming addiction, binary Logistic regression analysis was conducted, controlling for demographic variables such as gender and age, to clarify the predictive effect of ADHD symptoms on the risk of internet gaming addiction.

The prevalence rate of internet gaming addiction among middle school students was found to be 4.57% (n=96). Latent profile analysis identified three potential subtypes of ADHD symptoms: low symptom subtype (n=1 058, 50.4%), moderate symptom subtype (n=829, 39.5%), and high symptom subtype (n=213, 10.1%). Further analysis indicated statistically significant differences in internet gaming addiction scores among the ADHD symptom subtypes (χ²=547.438, P<0.001), with the high symptom subtype scoring the highest, followed by the moderate symptom subtype, and the low symptom subtype(scores were 26.79±0.54, 19.47±0.26, 14.75±0.19, respectively). After adjusting for demographic variables such as gender and ethnicity and conducting multiple comparisons, the association between moderate and high levels of ADHD symptoms and the risk of internet gaming addiction remained significant (OR values respectively were (1.25, 95% CI:1.58-1.35) and (1.14, 95% CI:1.08-1.20)), while the impact of the low symptom level was not significant.

There is significant heterogeneity in ADHD symptoms among middle school students and different latent subtypes of ADHD symptoms are significantly associated with internet gaming addiction. Students with higher levels of ADHD symptoms are more likely to have higher internet gaming addiction scores. Schools and families should pay more attention to middle school students exhibiting more severe ADHD symptoms to promote mental health development.

To understand the successful aging of the rural elderly in China and analyze its influencing factors.

The rural elderly aged 60 and above from the 2018 China Health and Retirement Longitudinal Study (CHARLS) were selected as the research object, it was determined that successful aging is achieved by simultaneously meeting the four dimensions of " no major chronic diseases"," no depression"," good cognitive and physical function" and "active social participation". Then multifactor logistic regression was used to analyze the successful aging of the rural elderly based on the social ecosystem theory.

The successful aging rate of the elderly in rural China was 6.79%. The results of multivariate logistic regression showed, self-rated health status in the hierarchy of personal characteristics (OR=0.426, 95% CI: 0.294-0.615), hearing (OR=0.605, 95% CI: 0.447-0.819), childhood health status (OR=0.635, 95% CI: 0.450-0.897), level of education (OR=7.571, 95% CI: 4.091-14.011), and personal annual income (OR=3.422, 95% CI:1.952-6.001), physical exercise in the individual behavioral hierarchy (OR=0.525, 95% CI: 0.291-0.948), daily duration of sleep (OR=0.615, 95% CI: 0.449-0.843), marriage status in the interpersonal level (OR=0.565, 95% CI: 0.349-0.917), financial support from children (OR=1.427, 95% CI: 1.053-1.934), internet use (OR=0.469, 95% CI:0.271-0.814), life satisfaction at the community level (OR=0.352, 95% CI:0.161-0.769), endowment insurance(OR=0.491, 95% CI: 0.245-0.983) in the policy level both were influencing factors for the successful aging of the elderly people in rural areas.

The degree of successful aging of the rural elderly in China is low, and good cognitive and physical functions and positive social participation dimensions are the main shortcomings for the successful aging of the rural elderly.

To understand the changes of stunting among primary and secondary school students in the areas of Nutrition Improvement Programme for rural Compulsory Education Students in Henan Province from 2012 to 2021, so as to provide a basis for improving malnutrition and promoting healthy growth of students.

A stratified random cluster sampling method was used to select 10% of primary and junior high schools from 28 monitoring counties in Henan Province, with no less than 40 students in each grade, a total of 1 198 228 students were sampled in 8 years, and their heights were measured to analyse the stunting situation.

The rates of stunting among students in the monitoring areas of Henan Province were 1.33%, 1.23%, 1.08%, 0.74%, 0.87%, 0.71%, 0.80% and 0.43% from 2012 to 2021, showing a fluctuating downward trend. The overall rate was higher for girls (1.09%) than for boys (0.85%) (χ²=189.596, P<0.05), and for primary schools (0.99%) than for lower secondary schools (0.79%) (χ²=61.862, P<0.05). The rates of stunting in both boys and girls, in primary and junior high school, and in all age groups basically showed a fluctuating downward trend among different years (P<0.05). The rate of stunting in all years showed a decreasing and then increasing trend in the age group of 6～12 years old, then an increasing trend after a decrease in the age group of 13 years old, and then a maximum in the age group of 16 years old.

The rate of stunting among students in the Nutrition Improvement Programme for Rural Compulsory Education Students in Henan Province from 2012 to 2021 shows a fluctuating downward trend, but there is still a certain percentage, which requires targeted intervention policies.

To systematically review the willingness to use pre-exposure prophylaxis (PrEP) and related factors among men who have sex with men (MSM) in China.

CNKI, VIP, Wanfang Data, CBM, EMbase, CINAHL, Pubmed, The Cochrane Library and Web of Science were searched from inception to August 16, 2023.

A total of 31 articles with 31 390 participants were included. The results of meta-analysis showed that the awareness rate of PrEP was 46.70% (95% CI: 34.14-59.47), the willingness rate of PrEP use was 61.49% (95% CI: 51.58-70.95), and the use rate of PrEP was 3.41% (95% CI:0.07-10.62). Age≥40 years (OR=1.90, 95% CI:1.47-2.57), HIV testing history (OR=2.13, 95% CI:1.63-2.80), awareness of PrEP (OR=2.80, 95% CI:1.86-4.24), online sexual partner seeking (OR=1.44, 95% CI:1.04-2.00), no regular sexual partner (OR=1.69, 95% CI:1.32-2.17), oral sex/anal sex behavior (OR=1.86, 95% CI:1.32-2.61), number of homosexual anal sex partners in the past 6 months>5 (OR=1.86，95% CI:1.32-2.61), Having oral or anal sex in the past year (OR=1.40，95% CI:1.19-1.65) was the promoting factor of PrEP use, while uncertain sexual orientation (OR=0.60, 95% CI:0.41-0.89) and always using condom in the past 6 months (OR=0.47, 95% CI:0.29-0.75) were the barriers.

The rate of MSM PrEP awareness and willingness to use is high in China, but the rate of PrEP use is low; more factors influence the willingness to use PrEP.