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To investigate the role of RIPK1/MLKL/PGAM5 pathway in traffic-related PM2.5-induced heart injury in asthmatic mice.
In total 50 male BALB/c mice were randomly divided into control group (NS group), asthma group (OVA group), OVA+ low concentration PM2.5 group (LPM2.5, 1.8 mg/ kg ·bw), OVA+ medium concentration PM2.5 group (MPM2.5,3.6 mg/ kg ·bw), and OVA+ high concentration PM2.5 group (HPM2.5, 7.2 mg/ kg ·bw). OVA asthma model mice were instilled with different concentrations of PM2.5 suspension through nasal cavity for 10 times. The pathological changes of heart tissue were observed by HE staining, the apoptosis of heart tissue was detected by Tunnel staining, and the protein and mRNA expression levels of RIPK1, RIPK3, MLKL, PGAM5, DRP1, and mRNA in mouse heart tissue were detected by qRT-PCR and Western blot methods.
Compared with NS group, the arrangement of central muscle fibers in PM2.5 group was obviously disordered, the myocardial fibers in HPM2.5 group were obviously broken, and the number of apoptotic cells was significantly increased. The protein expression of RIPK1, RIPK3, MLKL, PGAM5, DRP1, and p-DRP1 in HPM2.5 group was higher than that in NS group (q1=20.940, P < 0.05; q2=7.311, P < 0.05; q3=4.805, P < 0.05; q4=5.976, P < 0.05; q5=5.096, P < 0.05; q6=10.390,P < 0.05). The expression of RIPK1, RIPK3, and DRP1 genes in HPM2.5 group was significantly higher than that in NS group (q1=7.952, P < 0.05; q2=5.172, P < 0.05; q3=5.430, P < 0.05).
Traffic-related PM2.5 can induce cardiac necrotic apoptosis in asthmatic mice through RIPK1/MLKL/PGAM5 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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