To investigate the role of ferroptosis in striatum damage induced by combined exposure of microplastics and manganese by establishing a mouse model following microplastics and manganese alone or combined exposure.

Forty SPF male C57 mice were randomly divided into control group, manganese exposure group, microplastics exposure group and combined exposure group. Neurobehavioral tests including rotarod test, open field test and sucrose preference test were performed. The contents of divalent iron, malondialdehyde and glutathione in striatum of mice were detected by using the kit. Western blotting was used to detect the protein expressions of SLC7A11, GPX4 and FPN1 in striatum of mice. Real-time PCR was performed to detect the expression of miR-124. Single factor analysis of variance was used for comparison of multiple groups of data, and LSD test was used for further pairwise comparison.

Behavioral tests showed that the residence time of the rotating rod (all P<0.001), the residence time in the central zone of open field test (all P<0.001), total distance of open field test (all P<0.001) and the sucrose preference rate (P_manganese=0.010; P_{microplastics}<0.001) in manganese exposure group and microplastics exposure group decreased significantly compared with that of the control group. Compared with the manganese exposure group and microplastics exposure group, the residence time of the rotating rod, the residence time in the central zone of the open field test and the total distance of the open field test of combined exposure group decreased significantly (all P<0.001). In addition, the sucrose preference rate of the combined exposure group was lower than that of the manganese exposure group (P=0.001). At the same time, compared with the control group, the contents of Fe²⁺ in striatum of mice in manganese exposure group and microplastics exposure group increased (P_manganese=0.002; Pmicroplastics=0.001), the contents of GSH decreased (P_manganese=0.015; Pmicroplastics<0.001). The content of MDA in the striatum of mice in microplastics exposure group increased (P<0.001). Furthermore, the contents of Fe²⁺ and MDA in striatum of mice in combined exposure group were higher than those in manganese exposure group (P_Fe²⁺=0.004; P_MDA<0.001) and microplastics exposure group (P_Fe²⁺=0.008; P_MDA=0.007), while the content of GSH was lower than that in the manganese exposure group (P<0.001). The protein expressions of SLC7A11 in manganese exposure group (P=0.010) and microplastics exposure group (P=0.001) were lower than that in the control group. The protein expression of GPX4 in microplastics exposure group was lower than that in the control group (P=0.007), and the protein expression of GPX4 in the combined exposure group was lower than that in the manganese exposure group (P=0.006). The protein expressions of FPN1 in the manganese exposure group (P=0.005) and microplastics exposure group (P=0.002) were lower than that in the control group and the protein expression of FPN1 in the combined exposure group was significantly lower than those in the manganese exposure group (P=0.008) and microplastics exposure group (P=0.005). Compared with the control group, the expressions of miR-124 in striatum of mice in manganese exposure group (P=0.002) and microplastics exposure group (P<0.001) increased. At the same time, the expression of miR-124 in the striatum of mice in the combined exposure group was higher than those in the manganese exposure group and microplastics exposure group (all P<0.001).

Combined exposure of microplastics and manganese might lead to striatum damage in mice through the regulation of ferroptosis, and then cause motor dysfunction and anxiety-depression-like behavior.

To analyze the co-morbidity of spinal curvature abnormality and myopia and the relevant factors in primary and middle school students in Guangzhou from 2019 to 2023, so as to provide references for the establishment of the mechanism of Co-morbidity, Shared Etiology, and Shared Prevention of common diseases in students.

From September 2019 to 2023, used stratified random cluster sampling, 4 248, 4 230, 4 266, 25 476, 25 587 primary and middle school students were selected as subjects for spinal curvature abnormality and myopia screening and questionnaire survey. The χ²test was used for comparing the group differences and univariate analysis. The multivariable logistic regression model was used to explore the influencing factors of their co-morbidity.

the co-morbidity rates of spinal curvature abnormality and myopia were 2.96%-6.00% from 2019 to 2023 in primary and middle school students in Guangzhou. The differences in the co-morbidity rates among students of each phases of studying and the difference of the co-morbidity rate in primary and middle school students over the past 5 years were statistically significant (P<0.05). Multivariate logistic regression analysis showed that low BMI junior and senior high school students (OR=1.72, 1.89, 95%CI: 1.11-2.67, 1.47-2.41) were more likely to suffer these two diseases. Primary school students in grades 4 to 6 who had menarche or spermatorrhea (OR=1.77, 95%CI: 1.04-3.02), screening time 2-<3h or≥3h per day (OR=3.06, 2.28, 95%CI: 1.41-6.68, 1.02-5.19), outdoor activities time<2h per day (OR=2.28, 95%CI: 1.22-4.26), junior high school students with low BMI (OR=1.72, 95%CI: 1.11-2.67), whose teacher "never or occasionally" reminded reading and writing posture (OR=1.50, 95%CI: 1.06-2.12), who were not strict with requiring standing and sitting posture themselves (OR=2.51, 1.99, 95%CI: 1.29-4.86, 1.06-3.74), senior high school students with low BMI (OR=1.89, 95%CI: 1.47-2.41) who had activities in the teaching building during recess (OR=1.33, 95%CI: 1.02-1.75), distance from chest to the edge of table "never or occasionally" more than 1 fist away during reading and writing (OR=1.27, 95%CI: 1.05-1.53) had a higher risk of spinal curvature abnormality and myopiaco-morbidity (P<0.05).

The co-morbidity of spinal curvature abnormality and myopia was associated with daytime outdoor exercise and sedentary behavior with bad posture. Early screening and early treatment of spinal curvature abnormality and myopia should be strengthened among adolescent students, especially in urban, female and emaciated students.

To analyze the impact of nighttime sleep duration on depressive symptoms in the lifestyle habits of elderly people with multiple comorbidities in China.

Using the fifth round of the China Health and Retirement Longitudinal Study project, the depression mood of elderly people with multiple comorbidities was evaluated based on the Center for Disease Control Depression Rating Scale (CSE-D-10). Nighttime sleep was divided into five levels:<6 h, 6 to <7 h, 7 to <8 h, 8 to <9 h, and ≥9 h. Multivariate logistic regression analysis was conducted to investigate the influencing factors of depression in elderly people with multiple comorbidities. Random forest model was used to analyze the importance of nighttime sleep in the risk of depression in elderly people with multiple comorbidities. Restrictive cubic spline curves were used to further explore the dose-response relationship between nighttime sleep duration and the risk of depression.

A total of 1 249 elderly individuals with multiple comorbidities were included, aged (68.51±5.91) years. Multivariate logistic regression analysis showed that the risk of depression in elderly individuals with multiple comorbidities who slept at night for 6 to <7 hours (OR=0.447 (95%CI: 0.309-0.646)), 7 to <8 hours (OR=0.550 (95%CI: 0.351-0.862)), and 8 to <9 hours (OR=0.498 (95%CI: 0.323-0.768)) was relatively lower compared to those who slept for less than 6 hours. Discomfort caused by pain, ADL, life satisfaction, and stroke are the influencing factors for depression in elderly people with multiple comorbidities (P<0.05). The results of the random forest model show that the importance score of nighttime sleep on depression symptoms is relatively high. The results of the restricted cubic spline curve indicate a non-linear relationship between nighttime sleep and the risk of depression symptoms. The risk of depression symptoms decreases with 6-8 hours of nighttime sleep, and increases with less than 6 hours or more than 8 hours of nighttime sleep.

Nighttime sleep has an impact on the occurrence of depression in elderly people with multiple comorbidities. It is recommended that elderly people maintain 6-8 hours of nighttime sleep to effectively reduce the risk of depression and have certain significance for early prevention and control of depression in the elderly population.

To evaluate the information quality of hypertension-related videos on the TikTok short video platform and assess their potential value for public health education.

In June 2024, we searched the TikTok short video platform using the keyword "hypertension" selecting the top 200 videos and ultimately including 177 videos for quality evaluation. Structured content integrity assessment tools and the DISCERN video quality assessment tool were used to evaluate the videos. The correlation between video quality and characteristics (duration, likes, comments, favorites, and shares) was studied.

The 177 videos received 11 250 432 likes, 366 588 comments, 3 265 929 favorites, and 3 780 513 shares. The median video length was 111 seconds (74, 158), and the median upload days were 672 days (147, 878). 89.3% of the videos were posted by doctors, 8.5% by individual science communicators, and 2.3% by news agencies. 98.9% of the videos had subtitles, all featured individuals, and only 26.0% included animated demonstrations. Content integrity scores were: definition 0.19±0.40, symptoms 0.11±0.32, risk factors 0.29±0.50, assessment 0.36±0.45, management 0.81±0.47, and outcomes 0.63±0.58. 90.4% of the videos had a DISCERN score of ≤50, indicating "average" or below quality, with no significant difference in DISCERN scores among different publishers (P>0.05). DISCERN scores were significantly correlated with video duration (r=0.409, P<0.001).

The overall quality of hypertension-related videos on TikTok is low, with a significant positive correlation between video duration and quality. High-quality videos receive less attention, making TikTok an unsuitable platform for hypertension education due to low information quality and reliability.

To assess the body mass index (BMI), percentage of body fat (PBF), and bone age of school-age children in Xuzhou city, and to explore the relationship between BMI, PBF, and bone age, so as to provide reference for promoting the health of children.

A cluster sampling method was used to select 601 students of grade three from three schools in Xuzhou from July to August 2023, questionnaire surveys and physical examinations were used to collect children’s data, and disordered multi-classification logistic regression analysis was used to explore the association between different types of BMI or PBF and bone age development.

The rates for thinness, overweight, and obesity in boys were 14.2%, 11.1%, and 19.2% respectively, in girls were 17.6%, 9.3%, and 14.0%, respectively; Multivariate logistic regression showed that after adjusting for covariates such as gender, development of secondary sexual characteristics, average monthly household income, birth weight, father’s height, and mother’s height, the risk of early bone age development was significantly increased in both the BMI obese and overweight groups compared to the BMI normal group (OR=10.10, 95%CI:4.83-21.12; OR=3.78, 95%CI:1.53-9.33); The PBF high body fat group had a significantly increased risk of early bone age development compared to the PBF normal group (OR=3.79, 95%CI:2.01-7.13); BMI obese and PBF high body fat groups are protective factors for delayed bone age development (OR=0.40, 95%CI: 0.20-0.78; OR=0.52, 95%CI:0.31-0.87).

Overweight and obese children are prone to premature bone age. It is recommended to regularly monitor weight, bone age and body composition while preventing and controlling overweight and obesity in children.

To observe the level and effect of medical insurance for five sites of cancer in China using an area of Anhui Province as an example.

Using the district’s Medicare billing list data for the three-year period 2019-2021. Based on the concepts of actual compensation ratio, bottom-line compensation ratio and catastrophic health expenditure, the level and effect of medical security were calculated by level, cost and level of medical institution, etc.

Overall, the out-of-pocket share of the patients was maintained at around 30%, the out-of-pocket share of patients with high medical expenses was small (16.59%). The level of tertiary medical institutions’ actual compensation ratio was low (65.81%-70.52%), but overallit still exceeded the bottom-line compensation ratio under the 40% and 25% catastrophic health expenditure standards.

The multi-level health insurance system has begun to bear fruit. There is a need to equalize the protection for different types of illnesses, to strengthen the hierarchical diagnosis and treatment, and to pay further attention to the needs of patients with “serious illnesses and light treatment”.

To analyze the epidemic characteristics of breakthrough cases of varicella in Shapingba District, Chongqing, and provide reference for the prevention and control of the varicella epidemic in the area.

Collect information on varicella cases and their vaccination in Shapingba District from 2019 to 2023, Use descriptive epidemiological methods to analyze the epidemiological characteristics of varicella breakthrough cases, and use linear regression to analyze the relationship between breakthrough cases and age at the time of vaccination and the time interval between vaccination and onset of disease.

From 2019 to 2023, there were a total of 802 breakthrough cases of varicella in Shapingba District, Chongqing, accounting for 14.87% of the total number of varicella cases. The majority of cases were vaccinated with one dose of varicella (648 cases, accounting for 80.80%), and the differences in composition among different years were statistically significant (χ²=16.400,P<0.005); The epidemic has a bimodal distribution, from October to December and May to July each year. The least cases are in winter (142 cases, accounting for 11.68%), and the most cases are in spring (198 cases, accounting for 17.32%). The composition differences between different seasons are statistically significant (χ²=16.348, P<0.001); The breakthrough cases of chickenpox are mainly male, with the highest incidence among primary school students aged 6～10, and the differences in composition are statistically significant (χ²=877.420, P<0.001); The time interval from the first dose of chickenpox vaccine to the onset of breakthrough cases was 46 days to 17 years, with a median \[M(P₂₅,P₇₅)\] of 7 years (4 years, 10 years). The median time interval between scattered children, primary school students, and middle school students was 3 years (2 years,4 years), 8 years (6 years, 9 years), and 12 years (11 years,14 years), respectively, with statistical significance (H=497.280, P<0.001); There is a negative correlation between age at the time of first dose vaccination and the interval between onset of the disease in breakthrough cases, with a correlation coefficient of r=-0.230 (t=-6.693, P<0.001).

Breakthrough cases of varicella in Shapingba, Chongqing are mainly concentrated in primary school students during the start of the school season, with an interval of 7 years between onset. It is necessary to further strengthen the prevention and control of the varicella epidemic in primary schools, further improve the vaccination rate of children with varicella vaccines, and recommend receiving two doses of vaccines before starting primary school to reduce the risk of breakthrough cases.

To establish an evaluation index system of medical service capacity in county hospitals, so as to provide a reference basis for further evaluating the overall medical service strength of county-level hospitals.

Experts were invited to evaluate the relative importance of indicators at all levels of medical service capacity, and the weights of the dimensions of the indicators system were determined using analytic hierarchy process.

A total of 35 experts were invited to consult letter inquiries, and the recovery rate of the questionnaire was 94.43%. The CR of first-level indicators judgment matrix was 0.0223, and the CR of the second-level index judgment matrix under the three dimensions of structure, process and outcome were 0.0000,0.0741and 0.0052, all less than 0.1. Finally, the evaluation index system included 3 first-level indicators, 10 second-level indicators and 23 third-level indicators. The highest weight of the first-level indicators was the result(0.4570). Among the combined weights there were 11 indicators that exceeded the average weight of 0.0435 (1/23).

The evaluation index system of county hospitals based on analytic hierarchy process is objective and reliable, which can provide reference for health administrative departments at all levels and county hospitals to carry out medical service ability evaluation and ability improvement according to the actual situation.

To understand the epidemiological characteristics of non-occupational carbon monoxide poisoning incidents in Beijing from 2018 to 2022, and to explore targeted prevention and control measures.

The data were from the National Public Health Emergency Reporting Management Information System. Descriptive epidemiological methods were used to analyze non-occupational carbon monoxide poisoning incidents in Beijing from 2018 to 2022. Relative risk (RR) was used to assess the risk of morbidity and mortality.

From 2018 to 2022, a total of 1841 non-occupational carbon monoxide poisoning incidents were reported in Beijing, with 2729 cases of poisoning and 31 deaths. The annual morbidity and mortality were 25.14 and 0.29 cases per million, respectively. There were two peaks of poisoning cases in spring and winter, accounting for 49.98% and 24.00% of the total cases, respectively. The highest incidence occurred between 8 and 11 o ’clock, accounting for 22.83%. In the inner suburbs, outer suburbs and urban areas, the incidence of non-occupational carbon monoxide was 0.96 cases/100 000, 1.27 cases/100 000 and 18.79 cases/100 000, and the mortality was 0.02 cases/100 000, 0.02 cases/100 000 and 0.11 cases/100 000, respectively. The poisoning incidents mainly occurred in residential families in 1633 cases, accounting for 88.70%; This was followed by 118 restaurants, accounting for 6.41 percent. The proportion of household poisoning incidents decreased from 92.10% in 2018 to 84.13% in 2022. The proportion of poisoning incidents in restaurants increased year by year from 4.23% in 2018 to 10.05% in 2022. The proportion of poisoning cases occurring at home decreased from 86.39% in 2018 to 71.84% in 2022. The proportion of poisoning cases in restaurants increased from 9.88% in 2018 to 20.57% in 2022.

The incidence and death risk of non-occupational carbon monoxide poisoning in Beijing is not balanced among regions, and the proportion of poisoning incidents in restaurants is increasing year by year. Targeted measures should be taken to strengthen the prevention and control work in the outer suburbs and key restaurants.

To explore the relationship between social media addiction and anxiety among college students and the role of bedtime procrastination and stress perception.

A total of 15493 college students were selected by convenience sampling in September 2023 with using the Bergen Social Networking Addiction Scale, the Bedtime Procrastination Scale, the Chinese Perceived Stress Scales and the Generalized Anxiety Disorder 7-item Scale. SPSS25.0 macro program PROCESS was used to analyze the mediating effect of bedtime procrastination and the moderating effect of stress perception.

The social media addiction was positively predicted college students’ anxiety (β=0.307, P<0.001). Bedtime procrastination partially mediated the relationship between social media addiction and anxiety in the college students. The mediation effect value was 0.060 (95% Bootstrap CI=[0.055-0.065]), the mediating effect accounted for 19.54% of the total. Stress perception moderated the relationship between bedtime procrastination and anxiety (β=0.094, P<0.001). As the level of perceived stress increased, the positive association between bedtime procrastination and anxiety became stronger.

Social media addiction can directly affect anxiety in the college students, or indirectly affect it through bedtime procrastination, and perceived stress moderates the path from bedtime procrastination to anxiety in the mediation model.