To explore factors influencing suicidal behavior in patients with severe mental disorders.

Using cohort data from a western region of China (2006-2018), 332 298 patients were included. A multilevel logistic regression model was used to analyze factors associated with suicidal behavior (suicide death and suicide attempts).

Among 332 298 individuals, 3 358 experienced the outcome event, with an incidence of 1.01%. Multilevel logistic regression showed that female sex (OR=1.142, 95%CI: 1.058-1.234), other ethnicities (OR=1.609, 95%CI: 1.220-2.122), and non married status (OR=1.169, 95%CI: 1.064-1.285) were associated with higher risk, while age ≥46 years (46-60: OR=0.754, 95%CI: 0.669-0.849; 61-90: OR=0.708, 95%CI: 0.615-0.814) was associated with lower risk. When analyzing suicide attempts and suicide death separately, gender, ethnicity, and marital status were associated only with suicide attempt, whereas urban/rural residence and family history were associated only with suicide death. Follow-up time showed opposite effects on the two outcomes.

Prevention efforts should target high-risk populations, including those with risk assessment levels 3-5, other ethnicities, adverse drug reactions, ongoing pharmacotherapy, and higher education levels. Maintaining follow-up and establishing an integrated prevention network combining clinical and social factors is essential.

To explore the differences in the composition and function of intestinal microbiota among healthy individuals, patients with diabetes, and those with diabetic nephropathy, and to analyze the characteristics of intestinal flora imbalance in patients with advanced diabetic nephropathy, providing references for the mechanism research and prevention and treatment strategy formulation of this disease.

A total of 32 patients with advanced diabetic nephropathy, 79 patients with diabetes mellitus and 55 healthy controls were enrolled from the West China Fourth Hospital and Southwest China natural population cohort. Clinical data were collected and fecal samples were subjected to metagenomic sequencing. Linear discriminant analysis was used to screen for differentially abundant species and functional pathways. Linear regression analysis was performed to investigate the correlation between population phenotypes and differentially abundant species. Random forest and SPIEC-EASI algorithms were used to establish species classifiers and interaction networks, respectively.

There were significant differences in the β-diversity of the intestinal microbiota among the three groups, but no statistically significant differences were observed in the α-diversity. In the advanced stage of diabetic nephropathy patients, the intestines were enriched with various opportunistic pathogenic bacteria (such as Eggerthella lenta, Clostridium innocuum, Hungatella hathewayi, etc.) and harmful pathways such as anaerobic energy metabolism and L-histidine degradation, and were significantly correlated with renal function indicators. The intestines of diabetic patients were characterized by the enrichment of opportunistic pathogenic bacteria (such as Escherichia coli and Klebsiella pneumoniae) and the activation of glycolytic pathways. The random forest model performed best in differentiating the diabetic nephropathy group from the healthy control group (AUC=0.920). The co-abundance network analysis further revealed that the intestinal microbial interaction network of diabetic nephropathy patients was significantly sparser and the topological parameters changed, suggesting that there was an imbalance in the microbial ecological network during the disease process.

From diabetes to the advanced stage of diabetic nephropathy, the intestinal microbiota shows a progressive functional disorder, with an increase in the abundance of harmful bacterial species and metabolic pathways, which is closely related to the progression of the disease.

To investigate the prevalence of comorbidity status of hypertension, diabetes mellitus and dyslipidemia (commonly referred to as the “Three Highs”), to identify factors influencing this comorbidity among community-dwelling residents aged 35–75 years in two districts of Jinan City, and to provide scientific evidence to inform targeted and precise management and intervention strategies for local patients with these metabolic comorbidities.

From September 2016 to June 2022, stratified cluster sampling was used to select residents aged 35–75 years in Licheng District and Zhangqiu District of Jinan. Questionnaire surveys, physical examinations, and blood tests were conducted. R 4.2.1 software was used for chi-square test, trend chi-square test, and binaryl logistic regression analysis to explore the influencing factors of “three highs” comorbidity.

A total of 30 214 participants were included, with 12 185 males (40.33%) and 18 029 females (59.67%), and a mean age of (57.97±9.55) years. The standardized prevalence rate of hypertension is 45.39%, that of diabetes mellitus is 22.54%, and that of dyslipidemia is 27.31%. Among them, the standardized comorbidity rate of the “three highs” stands at 5.95%. The highest standardized comorbidity rate is observed in patients with both hypertension and hyperlipidemia, reaching 9.75%. Binary logistic regression analysis showed that the following factors were risk factors for “three highs” comorbidity (all P<0.05): male (OR=1.121 95%CI: 1.028-1.222), older age groups (OR≥1.992 in all age groups); administrative/professional and technical personnel (OR=1.551 95%CI: 1.120-1.989), retired/unemployed/household workers (OR=1.174 95%CI: 1.041-1.324), private business owners/other personnel (OR=1.427 95%CI: 1.101-1.850); history of cardiovascular and cerebrovascular diseases (OR=1.943 95%CI:1.620-2.332); overweight (OR=1.624 95%CI: 1.418-1.859); obesity (OR=2.016 95%CI: 1.766-2.404); and central obesity (OR=1.931 95%CI:1.733-2.152).

In Jinan City, the comorbidity prevalence of hypertension, diabetes, and dyslipidemia is relatively high, with hypertension combined with dyslipidemia being the most common comorbidity pattern. Priority interventions should be targeted at males, the elderly, sedentary occupational groups, individuals with a history of cardiovascular and cerebrovascular diseases, and overweight/obese individuals.

To retrospectively analyze the epidemiological characteristics and treatment outcomes of patients with recurrent pulmonary tuberculosis in Shaanxi Province from 2011 to 2024, so as to provide a scientific basis for the prevention and control of recurrent pulmonary tuberculosis and the formulation of relevant strategies in Shaanxi Province.

The medical records of all patients with recurrent pulmonary tuberculosis registered in the Tuberculosis Information Management System (TIMS) of Shaanxi Province from 2011 to 2024 were collected. Descriptive epidemiological methods were used to analyze the epidemiological characteristics of the subjects, chi-square test was used for inter-group comparison, and Joinpoint regression model was applied for time trend analysis.

A total of 11 715 patients with recurrent pulmonary tuberculosis were cumulatively reported in Shaanxi Province from 2011 to 2024. The registration rate of recurrent pulmonary tuberculosis in the province decreased from 3.94/100 000 population in 2011 to 1.46/100 000 population in 2024, showing an overall significant downward trend (AAPC=-8.6%, P<0.05). The composition of patient detection sources underwent a fundamental shift: the dominant detection route changed from “direct consultation” (accounting for 52.45% in 2011) to “referral”(with the proportion peaking at 73.13% in 2020). During the study period, the cumulative etiologically positive rate of recurrent patients was 56.18%, which was significantly higher than 35.91% of all notified pulmonary tuberculosis patients in the general population, with a statistically significant difference (χ²=1 983.846, P<0.05). The demographic characteristics showed that patients with recurrent pulmonary tuberculosis were predominantly concentrated in male farmers aged 40-64 years, accounting for a cumulative 36.46% of all enrolled subjects. Meanwhile, although the average annual consultation delay rate of recurrent patients was 56.93%, it showed a significant continuous downward trend during the study period (AAPC=-2.50%, P<0.05). Treatment outcome analysis showed that the cumulative treatment success rate of patients with recurrent pulmonary tuberculosis from 2011 to 2024 was 82.41%, while Joinpoint regression indicated a significant downward trend of this rate (AAPC=-1.43%, P<0.05).

The epidemic of recurrent pulmonary tuberculosis in Shaanxi Province has shown an overall downward trend, but the etiologically positive rate is significantly higher than that of all notified pulmonary tuberculosis patients, and the composition of patient detection sources has changed dramatically. Recurrent patients are concentrated in middle-aged and elderly male farmers, and the treatment success rate is on a downward trend, suggesting that there are still weak links in the treatment and management of recurrent patients. It is recommended to strengthen active screening and health management for high-risk groups such as farmers, optimize the referral network to reduce consultation delay, and improve the quality of treatment and management for recurrent patients to increase the treatment success rate.

To investigate the mechanism by which reduced degradation of Bcl-2/adenovirus E1B-19-kDa interacting protein 3 (BNIP3) mediated by neural precursor cell expression developmental downregulation protein 4 (NEDD4) and proteasome β5 subunit (PSMB5) contributes to impaired autophagy flux and cellular damage in arsenic-induced human hepatocellular carcinoma cells (HepG2).

The cytotoxic effects of different doses of sodium arsenite (NaAsO₂) treated HepG2 cells over varying time periods were evaluated by analyzing cell morphology, ultrastructure, cell viability, and hepatic injury enzyme levels (ALT, AST). Immunoprecipitation blotting (Western blot) was used to detect the expression levels of NEDD4, PSMB5, BNIP3, and autophagy-related proteins. Co-immunoprecipitation (Co-IP) experiments were conducted to analyze the interactions among NEDD4, PSMB5, and BNIP3. Specific inhibitors including cycloheximide (CHX), chloroquine (CQ), and proteasome inhibitor (MG132) were employed to inhibit protein synthesis, lysosomal function, and the ubiquitin-proteasome pathway, respectively, to investigate the roles of the ubiquitin-proteasome pathway and autophagy in arsenic-induced HepG2 cell damage.

As the concentration and duration of NaAsO₂ exposure increased, the viability of HepG2 cells decreased and morphogenesis exhibited significant changes. Additionally, autophagy flux was impaired, and cellular damage worsened in a dose- and time-dependent manner. Different concentrations and durations of NaAsO₂ exposure down - regulated NEDD4 and PSMB5 protein expression while up - regulating BNIP3, Beclin-1, P62, and LC3II/LC3I protein expression, showing time - effect and dose - effect relationships (P<0.05). CQ intervention exacerbated NaAsO₂ - induced autophagy flux obstruction and cellular damage (P<0.05). Co-IP and CHX experiments demonstrated that NaAsO₂ exposure reduced the interaction between NEDD4 and PSMB5 with BNIP3, thereby decreasing BNIP3 degradation (P<0.05). MG132 intervention downregulated PSMB5 protein expression and up - regulated BNIP3, P62, Beclin-1, and LC3II/LC3I protein expression, with all differences being statistically significant (P<0.05).

NaAsO₂ exposure can reduce the expression of NEDD4 and PSMB5 in HepG2 cells in a time - and dose - dependent manner, pregulate BNIP3 protein levels, and impair autophagy flux, ultimately leading to cellular damage. The mechanism may involve reduced binding of NEDD4 and PSMB5 to BNIP3, leading to decreased BNIP3 degradation and consequent cumulative accumulation.

To investigate the therapeutic efficacy and underlying mechanism of ZSP1603, a novel independently developed small-molecule tyrosine kinase inhibitor, against lung tissue injury in silicotic mice, so as to provide an experimental basis for clinical research on the treatment of silicosis.

Based on the molecular toxicological network induced by silica dust, molecular docking was performed to predict the potential targets and mechanisms of ZSP1603 in alleviating silica dust-mediated respiratory toxicities. A silica-induced mouse silicosis model was established. Pathological staining, immunohistochemistry and multi-omics analyses were comprehensively applied to evaluate the protective effects and molecular mechanisms of ZSP1603 on silica-associated silicosis-like lesions in mice.

A total of 50 intersecting genes were identified between the pharmacological targets of ZSP1603 and the toxicity-related genes triggered by silica dust, which were mainly enriched in signaling pathways associated with inflammation, oxidative stress and fibrosis. Long-term administration of ZSP1603 at the experimental dose (60 mg/kg) significantly ameliorated silica-induced pulmonary inflammation, lung tissue damage and collagen deposition (at 12 weeks, the Szapiel and Ashcroft scores in the ZSP1603 group were significantly lower than those in the model group, P<0.01), and downregulated the expression of proteins related to silica toxicity (the expression levels of Collagen I, Collagen III, α-SMA, FN, TNF-α, IL-6, and IL-1β were significantly downregulated compared to the model group, P<0.001). Moreover, ZSP1603 effectively corrected the gut microbiota dysbiosis in silicotic mice. In addition, no obvious systemic in vivo toxicity was observed following treatment with the experimental dose of ZSP1603.

The novel tyrosine kinase inhibitor ZSP1603 alleviates silicosis-related inflammation and fibrosis in a mouse silicosis model. These findings provide a reference for the research and development of therapeutic agents for the secondary and tertiary prevention and clinical intervention of silicosis.

To identify the main chemical occupational hazard factors and their exposure levels associated with condensate oil in natural gas exploitation, and to explore occupational health risk assessment methods for chemical hazards in this industry, so as to provide a basis for accurately preventing occupational hazards and protecting the health of occupational populations.

Based on occupational health surveys, condensate oil component analysis and continuous monitoring of chemical occupational hazards, the occupational health risks of condensate oil exposure in natural gas exploitation were assessed using the comprehensive index method, ICMM quantitative assessment method and occupational hazard risk index method.

The main occupational hazard factors of condensate oil in natural gas exploitation were benzene, toluene, xylene, n-hexane and cyclohexane. Their time-weighted average concentrations (C_TWA) ranged within 1%-50% of the occupational exposure limits (OELs). Among the 10 gas production well stations, only xylene concentration showed statistical significance (P<0.001), while concentrations of the other four chemical toxicants were generally consistent (P>0.05). Short-term exposure concentrations (C_STEL) of all five typical chemical toxicants were detected in the gas field water treatment station, with generally consistent concentrations (P>0.05). Analysis of occupational health risk levels showed that according to the comprehensive index method, benzene was rated as extremely high risk in both gas production stations and gas field water treatment stations, toluene, n-hexane and cyclohexane as high risk, and xylene as medium risk. According to the occupational hazard risk index method, benzene was moderate risk in the gas field water treatment station and mild risk in gas production stations, while all other factors were assessed as no risk. In the ICMM quantitative assessment method, only benzene in the gas field water treatment station was rated as intolerable risk, and all other factors were rated as tolerable risk. The Kruskal-Wallis test showed significant differences in the rating distributions among the three methods (P<0.05), indicating that different assessment systems varied in their ability to discriminate risk levels of hazardous factors.

Benzene, toluene, xylene, n-hexane and cyclohexane should be the key occupational hazards concerned in condensate oil exposure in the natural gas exploitation industry. The improved ICMM quantitative assessment is a suitable method for occupational health risk assessment of condensate oil in natural gas exploitation.

To explore the impact of early life factors on the initial establishment of infant gut microbiota and their association with allergic diseases in infancy.

A nested case-control study was conducted involving 82 samples from the maternal-infant cohorts established at West China Second University Hospital and Chengdu Women’s and Children’s Central Hospital.

In the vaginal delivery group, the incidence of allergic diseases in infants (30.30%) was lower than that in the cesarean section group (51.02%), with no statistically significant difference; in the breastfeeding group, the incidence (31.81%) was lower than that in the mixed feeding group (55.26%), and the difference was statistically significant (P=0.063; P=0.032). On day 0, the Simpson index in the allergic group was significantly higher than that in the healthy group (P<0.05). By day 30, the healthy group exhibited more favorable microbial richness-related indices of the gut microbiota. On day 7, the relative abundance of Bdellovibrionota in the allergic mixed-fed group was significantly higher than that in other groups (P<0.05). The relative abundance of Bifidobacterium in feces was significantly higher in the healthy group than in the allergic group on day 7 (P<0.05), and the abundance of Bifidobacterium in breast milk of the healthy group remained higher than that of the allergic group from day 0 to day 30.

Vaginal delivery is conducive to maintaining the diversity of gut microbiota in early infancy and establishing a gut microbiota pattern dominated by Bifidobacterium. Meanwhile, breastfeeding promotes the colonization of probiotics such as Bifidobacterium, thereby reducing the long-term risk of allergies in infants.

To explore the dynamic relationship between physical exercise and early adolescent insomnia disorder, and to examine the longitudinal mediating role of depressive symptoms in this relationship, providing scientific evidence for promoting the physical and mental health of adolescents.

A total of 1 162 students from four junior high schools in Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province were selected using cluster convenience sampling and participated in three follow-up surveys. The research tools included the Insomnia Severity Index (ISI), the Physical Activity Rating Scale (PARS-3), and the Patient Health Questionnaire-9 (PHQ-9) for assessing depressive symptoms. Cross-lagged panel modeling (CLPM) was used to analyze the dynamic relationship between physical exercise and insomnia disorder, and to test the longitudinal mediating effect of depressive symptoms.

The detection rates of depressive symptoms were 50.5%, 55.4%, and 48.1%, while those of insomnia disorder were 48.3%, 46.6%, and 50.1% at the three time points. Physical exercise significantly negatively predicted subsequent insomnia disorder (T1→T2:β=-0.087; T1→T3: β=-0.245, allP<0.001). Insomnia disorder also significantly negatively predicted subsequent physical exercise (T1→T2:β=-0.168; T2→T3:β=-0.213, allP<0.001). Depressive symptoms partially mediated the relationship between physical exercise and insomnia disorder (T1 physical exercise→T2 depressive symptoms:β=-0.150, P<0.001; T2 depressive symptoms→T3 insomnia disorder:β=0.110, P<0.001; indirect effect:β=-0.016, 95%CI=-0.025- -0.010, P<0.001). Physical exercise could indirectly affect insomnia disorder through multiple pathways(total indirect effect:β=-0.117,P<0.001).

There is a long-term bidirectional predictive relationship between physical exercise and early adolescent insomnia disorder, and physical exercise can indirectly influence the development of insomnia disorder through depressive symptoms. It is important to emphasize the cultivation of regular physical exercise habits and intervention of depressive symptoms in early adolescence to improve insomnia disorder and promote the physical and mental health development of adolescents.

To explore the prevalence of comorbidity of myopia and overweight/obesity among junior high school students in Handan and its association with sleep patterns, in order to provide scientific evidence for developing effective prevention measures.

From October to November 2025, a stratified cluster random sampling method was used to select 8 384 junior high school students from 18 counties (cities, districts) in Handan. Participant underwent physical examinations and complete questionnaire. Multivariate Logistic regression model was used to analyze the association between sleep and comorbidity of myopia and overweight/obesity, and restricted cubic spline model was used to clarify the dose-response relationship.

The detection rate of comorbidity of myopia and overweight/obesity among junior high school students in Handan was 16.78%. The median daily sleep duration was 8.42 hours, and the sleep sufficiency rate was 34.03%. Students with light in the sleep environment had a higher risk of comorbidity (OR=1.14, 95%CI:1.01-1.28). Taking the “early to bed and normal wake-up” mode as the reference, junior high school students with “early to bed and early wake-up” mode and “late to bed and normal wake-up” mode had a higher risk of comorbidity (OR=1.27, 95%CI:1.03-1.55; OR=1.33, 95%CI:1.08-1.65). The dose-response relationship between sleep duration and comorbidity risk was an inverted “J” shape. When the sleep duration was longer than 8.1h/d, the comorbidity risk gradually decreased with the extension of sleep duration.

The comorbidity of myopia and overweight/obesity among junior high school students in Handan is a serious issue. Sleep insufficiency is a widespread phenomenon highly associated with an increased risk of comorbidity with myopia and overweight/obesity, indicating that sleep health is a weak link in the current health management of students.