The Perth Alexithymia Questionnaire (PAQ) was translated into a Chinese version of the questionnaire and tested for reliability and validity in adolescents.

The Chinese version of the PAQ scale was formed according to the procedures of forward translation, back translation, cultural adaptation and pre-survey. During February and April 2024, 7 975 adolescents from Shandong Province, Anhui Province, and Hunan Province were selected to test the reliability of the Chinese version of the PAQ. The item analysis was performed using the coefficient of determination and correlation coefficient methods, and the reliability and validity were assessed using confirmatory factor analysis, criterion-related validity, internal consistency reliability, and test-retest reliability. The Toronto Alexithymia Scale, the Patient Health Questionnaire and the Generalized Anxiety Disorder Scale were used as validity scales, and 1 083 adolescents were retested 4 weeks later to determine the reliability of the retest.

The results of the confirmatory factor analysis showed that the Chinese version of the PAQ scale was a 5-factor model (GFI=0.921, NFI=0.938, CFI=0.940, TLI=0.931, RMSEA=0.060). The Cronbach α coefficient for the Chinese version of the PAQ total scale was 0.953. The Cronbach α coefficients for the five subscales of Negative-Difficulty Identifying Feelings, Positive-Difficulty Identifying Feelings, Negative-Difficulty Describing Feelings, Positive-Difficulty Describing Feelings, and General-Externally Orientated Thinking were 0.821, 0.852, 0.807, 0.834 and 0.859, respectively. The retest reliability of the Chinese version of the PAQ scale was 0.762. The retest reliabilities for the five subscales of Negative-Difficulty Identifying Feelings, Positive-Difficulty Identifying Feelings, Negative-Difficulty Describing Feelings, Positive-Difficulty Describing Feelings, and General-Externally Orientated Thinking were 0.717, 0.676, 0.716, 0.709 and 0.692, respectively. The total score of the Chinese version of the PAQ scale was positively correlated with the total score of the Toronto Alexithymia Scale, depressive symptoms, and anxiety symptoms (r=0.782, 0.378, 0.369, P<0.01).

The Chinese version of the PAQ scale showed excellent psychometric properties on all measures in the adolescent population and can be used as an important tool for assessing adolescents’ alexithymia.

To explore the construction of the training system for preventive medical professionals and train high-level public health talents.

West China School of Public Health, Sichuan University, has deeply promoted the reform of talent education and teaching for undergraduate students in preventive medicine, exploring from aspects such as training objectives, curriculum and teaching material systems, practical ability training, and the cultivation of medical prevention integration capabilities. We have reconstructed the talent cultivation program, innovated the talent cultivation mode, and explored the new paradigm of cultivating top-notch and innovative talents for the preventive medicine specialty.

We have revised and completed the general training plan for public health and preventive medicine, constructed a modernized curriculum system of preventive medicine, strengthened the development and application of digital education, actively explored the in-depth cooperation with practice teaching bases, and taken the lead in the country in launching the innovative class of hospital infection management, the dual bachelor’s degree of preventive medicine and software engineering, and the micro-specialty of health insurance.

Under the strategic background of Healthy China, professional education of preventive medicine is the key path for the cultivation of public health talents in China. Colleges and universities should actively explore the innovation and development of the cultivation of preventive medicine professional talents, so as to contribute to the construction of Healthy China in the new era.

To explore the impact of social participation on cognitive decline in middle-aged and elderly people, and to provide a reference for improving or maintaining the cognitive health of middle-aged and elderly people.

A total of 6 645 middle-aged and elderly people over 50 years old in the 2018 China Longitudinal Survey on Influencing Factors of Elderly Health (CLHLS) were selected as the research subjects, and cognitive frailty was evaluated by the Mini-Mental State Evaluation Scale (MMSE) and Frailty Index (FI). A multivariate logistic regression model was used to analyze the effects of social participation type and intensity on cognitive weakness.

The higher the participation of middle-aged and elderly people in family activities, individual activities, and group activities, the lower the incidence of cognitive weakness. High participation in family activities had the highest effect on mild (OR=0.271, 95%CI:0.228-0.321), moderate (OR=0.463, 95%CI:0.361-0.592) and severe (OR=0.079, 95%CI:0.043-0.146) cognitive frailty. The incidence of cognitive frailty was negatively moderated by high participation in family activities (OR=2.263, 95%CI:1.579-3.244), and the incidence of cognitive frailty was negatively moderated by urban middle-aged and elderly people in group activities (OR=0.459, 95%CI:0.289-0.727) and high participation (OR=0.490, 95%CI:0.331-0.725).

It is of great significance to increase the enthusiasm of middle-aged and elderly people for social participation, especially family activities, to delay the decline of cognitive frailty.

To explore the potential categories of primary caregiver burden in families of cancer patients and their potential related factors, and to provide scientific basis for more targeted policy interventions.

From February to April 2023, we conducted a questionnaire survey among the primary family caregivers of cancer patients at the Third Affiliated Hospital of Harbin Medical University using convenience sampling. Latent profile analysis was employed to identify potential categories of caregiver burden. The modified BCH method was used to assess the differences in family resilience, individual resilience, and caregiving ability across different burden categories.

A total of 367 participants were included in the study, and their caregiving burden was divided into two categories, named "mild caregiver burden group" and "moderate and severe caregiver burden group". The respondents of "moderate to heavy caregiver burden" were mostly female who were highly educated and had spouse and children. Different potential categories of family caregiver burden profiles showed statistically significant differences in family resilience (χ²=5.333，P=0.021), individual resilience (χ²=31.687，P<0.001), and caregiving ability (χ²=13.391，P<0.001).

There are heterogeneous subgroups of primary caregiver burden in families of cancer patients, and policy makers should implement targeted interventions to reduce the burden of primary caregivers for families with cancer patients, and to improve their personal and family resilience and caring abilities.

To investigate the status of drug resistance of Mycobacterium tuberculosis(M.tuberculosis) isolated from drug resistance monitoring stations in Changsha from 2021 to 2023, and to provide reference for the control effect of drug-resistant tuberculosis.

The positive specimens from designated medical institutions in Changsha were collected and sent to our hospital together with the basic information of patients for further M.tuberculosis drug susceptibility test. Drug susceptibility tests were performed on all samples by proportional method, including 8 drugs, such as RFP, INH, EMB, SM, OFX, PAS, TH, KM, etc, and the qualitative data were analyzed by χ² test.

Among the 3 037 isolates, 2 534 were fully sensitive and 503 were drug-resistant, and the total drug resistance rate was 16.56%. Among them, 316 were single drug resistant, and the drug resistance rate was 10.41%. There were 81 multi-drug resistant strains (2.67%) and 106 poly-drug resistant strains (3.49%). The drug resistance sequences of any drug were INH, SM and RFP, 9.02%, 6.98% and 3.65%, respectively. The top three of multi-drug resistant patterns were INH+RFP (0.66%), INH+RFP+SM (0.4%) and INH+RFP+SM+EMB (0.36%), respectively. The top three poly-drug resistant patterns were INH+SM (1.15%), INH+TH1321 (0.4%) and INH+EMB (0.2%), respectively. In addition, from year of 2021 to 2023, the resistance of M.tuberculosis to RFP, EMB, and TH1321 drugs in Changsha area showed a decreasing trend year by year (P<0.05), but the overall resistance rate of patients in Changsha urban area was significantly higher than that in other districts and counties.

Compared with other provinces and cities, the overall drug resistance rate of tuberculosis patients in Changsha is low, but the drug resistance rate of tuberculosis in Changsha urban area is significantly higher than that in other districts and counties, which should arouse the attention of relevant departments.

To explore the characteristics of HIV molecular transmission network of men who have sex with men (MSM) among newly reported HIV cases in Jinan, China.

The plasma samples of newly reported HIV-1 MSM in Jinan from 2022 to 2023 were collected. The pol region gene was amplified using nested PCR followed by sequencing. The obtained sequences were used to construct phylogenetic tree and molecular transmission network. The related factors for participants entering a cluster were analyzed. Drug resistance mutations were identified and analyzed using the online software of HIV drug resistance database of Stanford University.

This study obtained 207 sequences and identified 15 HIV-1 subtypes. The dominant subtypes were CRF07_BC (76/207, 36.7%) and CRF01_AE (74/207, 35.7%), followed by B subtypes (21/207, 10.1%), CRF114_0155 (9/207, 4.3%), and CRF55_01B (8/207, 3.9%). Setting 1.5% as the gene distance threshold, a total of 15 molecular clusters were formed by 42 sequences, with a transmission network entry rate of 20.3%. Multivariate Logistic Analysis results showed that MSM with college or above education level and CD4 count>500 cells/μl were more likely to enter the molecular transmission network. Among the 207 participants, 11(5.3%) were detected with drug resistance mutations, including 2 with PI resistance mutations, 6 with NRTI resistance mutations, 2 with NNRTI resistance mutations, and 2 with INSTI resistance mutations. One participant was detected with both NRTI and NNRTI resistance mutations.

The gene subtypes of newly reported HIV cases are diverse, mainly were CRF07_BC and CRF01_AE. MSM with younger age and higher education level are active populations in the HIV transmission network. HIV transmission network monitoring is a long-term and continuous work, and it is necessary to strengthen the traceability of key populations in the network and provide more effective intervention strategies to reduce the HIV transmission.

To investigate the association between the expression of mucin-5B(MUC5B) gene and its locus polymorphisms rs2075853, rs4963053, and rs2014486 with coal worker’s pneumoconiosis(CWP).

The study analyzed age and years of service from 506 subjects (comprising 143 healthy individuals, 132 dust-exposed workers, 121 stage I CWP patients, and 110 stage II CWP patients) collected at the Guiyang City Public Health and Medical Center from February 2020 to October 2023. Gene expression levels were assessed using real-time quantitative polymerase chain reaction（qRT-PCR）. Receiver operating characteristic(ROC) curves were plotted, and the area under the ROC curve (AUC) was calculated to evaluate the predictive value of MUC5B for CWP. Genotyping of MUC5B rs2075853, rs4963059, and rs2014486 was performed using a MassARRAY Analyzer, and the association between the three single nucleotide polymorphisms (SNPs) genotypes’ distribution frequency and the risk of CWP was analyzed.

Significant statistical differences were observed among the groups in terms of age (H=242.198, P<0.001), years of service (H=315.822, P<0.001). Expression levels of MUC5B were significantly reduced in stage I and II CWP patients compared to the healthy and dust-exposed groups (H=34.491, P<0.001). ROC curve analysis indicated that MUC5B could serve as an auxiliary diagnostic marker for CWP (AUC=0.755, 95% CI: 0.682-0.828, P<0.001). Furthermore, haplotype analysis revealed that the CG haplotype constructed from rs4963059 and rs2014486 was more frequent in CWP patients, showing a significant association (odds ratio OR=1.719, 95% CI: 1.074-2.751, χ²=5.201, P<0.05), suggesting that the CG haplotype constructed by rs4963059 and rs2014486 loci was associated with an increased risk of coal workers’ pneumoconiosis.

The CG haplotype constructed by MUC5B gene rs4963059 and rs2014486 is closely related to the risk of developing coal workers’ pneumoconiosis.

To analyze the influencing factors of the first visit of elderly people at the grassroots level in China, and further implementing graded diagnosis and treatment policies.

Based on the 2020 CLASS data, 4 444 elderly people were included, and a chi square test was used to analyze the influencing factors of their primary diagnosis behavior. A random forest model was used to rank the importance of variables with significant impact.

The elderly who living in the eastern region (OR=1.791,95% CI:1.421-2.256) and the central region (OR=1.438,95% CI:1.121-1.846), having a general self-evaluation (OR=1.869,95% CI:1.501-2.327) and self-assessment health (OR=2.342,95% CI:1.869-2.936), having two kinds of chronic diseases (OR=1.379,95% CI:1.174-1.621) and more than two kinds of chronic diseases (OR=2.648,95% CI:2.236-3.137), using health intelligent devices (OR=1.192,95% CI:1.032-1.377), using basic health services(OR=1.756,95% CI:1.527-2.020), college degree or above (OR=1.480,95% CI:1.088-2.012), and Internet access (OR=1.184,95% CI:1.015-1.381) are more willing to go to the grass-roots level for first diagnosis.

The first visit rate of elderly people at the grassroots level still needs to be improved. It is necessary to strengthen the publicity effect of grassroots first visit, improve the utilization rate of grassroots health services, encourage elderly people to use health intelligent devices, increase the construction of grassroots medical and health institutions in western regions, and correctly guide elderly people to form good medical habits.

To explore the effects of different sequential strength and endurance training on body composition and cardiorespiratory endurance of primary school students, and to provide theoretical basis for scientific guidance of primary school students’ fitness and improvement of children’s physical health. Subjects 60 boys in grade 5 of a primary school in Beijing (age: 10.22 y±0.73 y; height: 150.23 cm±9.63 cm; weight:45.71 kg±8.09 kg; BMI: 20.25 kg/m²±4.77 kg/m²).

The subjects were divided into three groups: simple endurance group (n=20), RE group (n=20) and ER group (n=20). Three groups of subjects were trained for 12 weeks, 3 times a week. Maximum oxygen uptake, body composition, lung function and other indicators were tested before and after intervention.One-way ANOVA and Paired Sample T-Test were used for statistical analysis.

After 12 weeks of exercise training intervention, there was no significant difference in the growth rate ofthe weight of the subjects between the three groups (Egroup: 2.78±7.23; REgroup: 4.00±7.20; ER groupin the same period: 3.47±7.94)(P>0.05).Compared with the simple endurance group, the percentage of physical fitness (Egroup: 10.69±3.34; RE group: 16.38±6.34; ER group: 23.15±10.30) was significantly decreased (P<0.01), and the ER group was better than the RE group (P<0.05). The muscle growth rate (Egroup: 6.84 ± 2.91; RE group: 12.58 ± 3.68; the ER group: 10.63±2.91) in the same period group was higher than the simple endurance group (P<0.01), and the RE group was higher than the ER group (P<0.05).The growth rate of vital capacity (Egroup: 12.71±4.64; RE group: 20.35±4.22; the ER group: 17.76±5.51) in the same period group was higher than that in the endurance group (P>0.05).There was no significant difference in the growth rate of vital capacity body mass index between the three groups (E group: 9.43±8.23; RE group: 16.55±7.96; the same period ER group: 13.44±10.26) (P>0.05).The maximum oxygen uptake growth rate (Egroup: 7.06±2.55; RE group: 14.10±4.12; the ER group: 11.26±4.04) in the same period group was higher than the endurance group (P>0.05).

Concurrent training is superior to simple endurance training in improving children’s body composition and cardiopulmonary endurance. The endurance-strength training sequence can effectively reduce the body fat content, the training sequence of strength-endurance can effectively increase the content of skeletal muscle and the improvement of children’s cardiopulmonary function is not related to the training sequence.

To evaluate the effectiveness of case study-modularized teaching design method in undergraduate nursing students.

Undergraduate nursing students in the class of 2020 of a medical university were selected as research subjects, and a parallel controlled trial was designed with case study-modularized teaching in the intervention group and traditional teaching methods in the control group, and the evaluation of teaching effectiveness was conducted by anonymous questionnaires and final grades.

The scores, learning attitudes, course cognition, teaching satisfaction, and self-learning effect evaluation of students in the intervention group were better than those in the control group (P<0.05), and the teaching method was especially effective in improving students’ comprehensive application ability.

The case study modularized teaching method used in the teaching of medical statistics is popular among students, with high course satisfaction and self-learning effect evaluation, which can improve the comprehensive application ability of students, and the teaching effect is remarkable.