PDF
Full
Outline
To investigate the association between the expression of mucin-5B(MUC5B) gene and its locus polymorphisms rs2075853, rs4963053, and rs2014486 with coal worker’s pneumoconiosis(CWP).
The study analyzed age and years of service from 506 subjects (comprising 143 healthy individuals, 132 dust-exposed workers, 121 stage I CWP patients, and 110 stage II CWP patients) collected at the Guiyang City Public Health and Medical Center from February 2020 to October 2023. Gene expression levels were assessed using real-time quantitative polymerase chain reaction（qRT-PCR）. Receiver operating characteristic(ROC) curves were plotted, and the area under the ROC curve (AUC) was calculated to evaluate the predictive value of MUC5B for CWP. Genotyping of MUC5B rs2075853, rs4963059, and rs2014486 was performed using a MassARRAY Analyzer, and the association between the three single nucleotide polymorphisms (SNPs) genotypes’ distribution frequency and the risk of CWP was analyzed.
Significant statistical differences were observed among the groups in terms of age (H=242.198, P<0.001), years of service (H=315.822, P<0.001). Expression levels of MUC5B were significantly reduced in stage I and II CWP patients compared to the healthy and dust-exposed groups (H=34.491, P<0.001). ROC curve analysis indicated that MUC5B could serve as an auxiliary diagnostic marker for CWP (AUC=0.755, 95% CI: 0.682-0.828, P<0.001). Furthermore, haplotype analysis revealed that the CG haplotype constructed from rs4963059 and rs2014486 was more frequent in CWP patients, showing a significant association (odds ratio OR=1.719, 95% CI: 1.074-2.751, χ2=5.201, P<0.05), suggesting that the CG haplotype constructed by rs4963059 and rs2014486 loci was associated with an increased risk of coal workers’ pneumoconiosis.
The CG haplotype constructed by MUC5B gene rs4963059 and rs2014486 is closely related to the risk of developing coal workers’ pneumoconiosis.
PDF
53
24
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
