To investigate the effects of blackcurrant on lipid metabolism and intestinal flora in High-Fat Diet mice.

A total of 150 C57BL/6J mice aged 7-8 weeks were selected, with half male and half female. Twelve mice were randomly selected and fed a normal diet as a blank control group. The other mice were fed a high-fat diet for 8 weeks, and the mice with successful obesity modeling were selected and randomly grouped according to gender. The 60 mice were divided into four groups, including high-fat（normal saline）, low-dose（1 250 mg/（kg·d））, medium-dose（2 500 mg/（kg·d））, and high-dose（5 000 mg/（kg·d））, with six mice in each group. The subjects were given continuous gavage for 10 weeks. To evaluate the effect of the intervention and explore the possible mechanism of the intervention. The results were described by (). Differences between groups were tested by ANOVA (α=0.05).

There were significant differences in liver weight, liver coefficient and kidney weight between ND female group and HFD female group and each subject group (P<0.05). The subcutaneous fat mass, perirenal fat mass, perirenal fat mass, and mesenteric fat mass in the ND male group were significantly different from those in the HFD male group and the LOW male group (P<0.05). The subcutaneous fat mass, perirenal fat mass and uterine fat mass of ND female mice were significantly different from those of HFD female group and LOW female group (P<0.05). There were significant differences in TC, TG, HDL-C and LDL-C between the ND male group and the HFD male group and the HIGH male group (P<0.05), and there were significant differences in TG and LDL-C between the ND female group and the HFD female group, the LOW female group and the HIGH female group (P<0.05). There was a significant difference in ALT among the groups (P<0.05). The cells of the liver tissue and adipose tissue in the ND group were intact, and the cells in the HFD group had large fat vacuoles, and the cells in each test group tended to return to normal levels. There was a significant difference in β-diversity among the four groups (P<0.01). The changes in the abundance of Firmicutes, Bacteroidota, and Verrucomicrobiota were also observed (P<0.05).

Blackcurrant can reduce body fat and visceral fat, regulating blood lipid metabolism, protect liver function and regulate intestinal flora.

To investigate the association between dietary oxidative balance score (DOBS) and the risk of sarcopenia in patients with hypertension.

Patients with hypertension aged 20 years and above who participated in four survey cycles of the National Health and Nutrition Examination Survey (NHANES), 2011-2018. Weighted logistic regression and restricted cubic spline were used to analyze the association between DOBS and the risk of sarcopenia in patients with hypertension, and subgroups analysis was performed according to gender and age.

A total of 2 775 patients with hypertension were included, with 349 (12.6%) diagnosed with sarcopenia. After adjusting for multiple covariates, higher DOBS were significantly associated with decreased risk of sarcopenia in patients with hypertension. For each one-point increase in DOBS, the risk of sarcopenia in patients with hypertension was reduced by 11.4% (OR=0.886, 95%CI: 0.842-0.933). As a categorical variable, the OR of DOBS Q3 group was 0.331 (95%CI: 0.116-0.941) and the OR of Q4 group was 0.124 (95%CI: 0.051-0.301) compared with Q1. A restricted cubic spline model indicated a linear negative dose-response association between DOBS and sarcopenia in patients with hypertension (P _non-linear=0.148). Subgroup analysis indicated no significant interaction of gender and age on the association of DOBS and sarcopenia in patients with hypertension (P _interaction > 0.05).

Higher DOBS is significantly associated with a lower risk of sarcopenia in patients with hypertension.

To investigate the association between newly developed non-alcoholic fatty liver disease (NAFLD) in the third trimester of pregnancy and adverse pregnancy outcomes, and to evaluate the necessity of prevention, monitoring, and health education for NAFLD during pregnancy.

This retrospective study included 3 505 singleton primiparous pregnant women without NAFLD in early pregnancy, registered at Beijing Friendship Hospital, Capital Medical University, from March 2020 to December 2022. Logistic regression analysis was used to investigate the association between newly developed NAFLD in the third trimester and adverse pregnancy outcomes, including cesarean section, preterm birth, small-for-gestational-age infants, large-for-gestational-age infants, and postpartum hemorrhage.

After adjusting for factors such as maternal age, gravidity, pre-pregnancy body mass index (BMI) level, gestational weight gain level, relevant medical histories, and educational level, the risk of postpartum hemorrhage was found to be increased in women with newly developed NAFLD in the third trimester (OR=2.136, 95%CI: 1.009-4.080, P=0.031). No statistically significant associations were found with cesarean section, preterm birth, small-for-gestational-age infants, or large-for-gestational-age infants.

A positive association between newly developed NAFLD in the third trimester of pregnancy and postpartum hemorrhage was revealed, highlighting the importance of strengthening surveillance and prevention for NAFLD during pregnancy to reduce the risk of postpartum hemorrhage.

To explore the impact of standardized clinical pathway management on the cost changes of anterior cruciate ligament injury of the knee and its value in medical practice, with the intention of providing experience for the reform of clinical pathways for disease types and medical insurance payment systems in the context of the DRG payment system.

The study selected a tertiary general hospital in City L as the research object and utilized the interrupted time series analysis method to compare the data before and after the implementation of clinical pathway management.

After the implementation, the average cost per case of this disease type significantly decreased, and the cost structure was optimized. The main cost items such as drug fees, examination fees, and treatment fees all showed a downward trend. The optimization of hospitalization frequency was limited, and the control of consumable costs still needed to be strengthened.

Although clinical pathway management has achieved certain results, it also faces challenges. During the implementation, it is necessary to be vigilant against the decomposition of hospitalizations, strengthen medical insurance supervision and technical monitoring, and optimize hospital management processes. Emphasis should be placed on the value of medical labor and the adjustment of salary systems. Continuous control of consumables is needed to control costs and optimize resource utilization. In the future, the system should be continuously improved to enhance service quality and efficiency, promote the high-quality development of public hospitals, and advance the reform of medical insurance payment systems.

To investigate the potential role of ferroptosis in the neurotoxicity induced by deltamethrin (DM) exposure.

Mice hippocampal neuronal cells HT22 cells were treated with DM at different doses (1, 2, 5, 10, 20, 50, 100, 200, and 400 μmol/L) for 24 h, cell survival rate was detected by MTT method to determine the optimal dose (2, 10, and 50 μmol/L). The cell morphology was observed by microscope. The level of lipid peroxidation was detected by flow cytometry. The level of glutathione (GSH) and the content of ferrous ion (Fe²⁺) were detected by the kit. The levels of ferritin heavy chain (FTH), light chain (FTL) protein and glutathione peroxidase 4 (GPX4) were detected by Western Blotting.

With the increase of DM dose, the number of HT22 cells gradually decreased, the morphology changed and the tentacles antennae broke. Compared with the control group, the survival rate of DM infected groups were significantly decreased (F=349.8, P<0.01), and the level of intracellular lipid peroxidation was significantly increased (F=14.86, P<0.01). With the increase of dose, GSH level in HT22 cells in 2, 10 and 50 μmol/L DM groups was significantly lower than that in control group (F=11.56, P<0.01), and Fe²⁺ level in 10 and 50 μmol/L DM groups was significantly higher than that in control group (F=17.67, P<0.01). Compared with the control group, GPX4 and FTL protein expressions in the 50 μmol/L DM group were significantly decreased with the increase of dose (F_GPX4=3.313, P<0.05; F_FTL=2.003, P<0.05), and FTH protein expressions in the 2, 10, 50 μmol/L DM groups were significantly increased with the increase of dose (F=16.95, P<0.01). Pretreatment with Fer-1, an ferroptosis inhibitor, for 2 h significantly reversed GSH, lipid peroxidation and Fe²⁺ levels in 50 μmol/L DM group (F_GSH=215.5, P<0.01; F_{lipid p eroxide}=17.97, P<0.01; F_Fe²⁺=23.26, P<0.01).

DM exposure induces ferroptosis in HT22 cells of mice hippocampal neurons leading to neurotoxicity, the mechanism of which may be related to intracellular lipid peroxidation and iron metabolism disorder.

To investigate the association between lipoproteins determined by nuclear magnetic resonance (NMR) and osteoarthritis (OA) using Mendelian randomization (MR).

Using the genome-wide association study (GWAS) of 116 NMR-based lipoproteins and total OA, knee OA, and hip OA, univariate MR analyses were conducted to assess the association between lipoproteins and osteoarthritis.

Univariate MR results showed that 73 lipoproteins were significantly associated with total OA, LDL diameter (OR=1.07, 95% CI: 1.03-1.11) and small HDL phospholipids (OR=1.05, 95% CI: 1.01-1.08) presented risk effects on total OA,while the remaining lipoproteins were protective and were distributed in apolipoprotein B, cholesterols, intermediate density lipoprotein, and very-low-density lipoprotein subclasses. 57 lipoproteins were significantly associated with knee OA, all of which were found in total OA, with LDL diameter (OR=1.11, 95% CI: 1.05-1.18) presenting a risk effect on knee OA, while the remaining lipoproteins were protective. 90 lipoproteins were significantly associated with hip OA, with 7 of which presenting risk effects, distributed among HDL cholesterol (OR=1.07, 95% CI: 1.01-1.18)and most of its medium- and large-sized subclasses, while the remaining lipoproteins were protective.

NMR-based lipoproteins were causally associated with OA, as their different sizes and lipid compositions play different roles for OA at different sites.

To analyze the influencing factors of hearing loss among workers in different workshop noise operations in a certain automobile manufacturing enterprise, and propose corresponding protective suggestions.

A stratified cluster sampling method was employed, and on-site questionnaire surveys were conducted to collect basic information, personal history, occupational history, and exposure status of the study subjects. Physical examinations were performed to obtain their hearing results. The study subjects were divided into four groups based on different workshops: the stamping workshop, the assembly workshop, the painting workshop, and the welding workshop. They were also categorized into two groups based on hearing: the normal hearing group and the hearing loss group. The relationship between various exposure characteristics and hearing loss was analyzed for 1 411 noise-exposed workers who met the inclusion and exclusion criteria, categorized by different workshops and hearing groups.

The detection rate of hearing loss in the research subjects was 20.13% (284/1 411), with the highest detection rate in the assembly workshop (25.00%). There was a statistically significant difference in the detection rate of hearing loss among different workshops (P<0.05). The differences in noise exposure levels and noise exposure duration among the four workshops were statistically significant (P<0.05); The detection rate of hearing loss in noise combined with organic solvent exposure was the highest (34.55%), significantly higher than that in noise combined with dust (21.69%), noise combined with other factors (20.00%), and noise alone (15.47%), with statistically significant differences between the groups (P<0.01). The detection rate of hearing loss in the noise combined with organic solvent exposure group was the highest in the final assembly and welding workshops, and the differences between different exposure groups in the two workshops were statistically significant (P<0.05). Logistic regression showed that workers exposed to noise combined with organic solvents and noise combined with dust had 2.67 times (P<0.01) and 1.43 times (P<0.05) higher risk of hearing loss, respectively, compared to those exposed to noise alone.

The exposure of noise combined with organic solvents and noise combined with dust in automobile manufacturing enterprises is the main influencing factor of hearing loss among workers. The detection rate of hearing loss in the final assembly workshop is the highest. Therefore, it is necessary to strengthen noise control and health protection in the workplace, and implement occupational health monitoring.

To evaluate the quality of life of the urban older adults with comorbidity from a multi-dimensional perspective, and to verify the influencing factors of the quality of life of the urban older adults with comorbidity by combining the theory of social embeddedness mechanisms.

The questionnaire data of 465 urban older adults with comorbidity were selected as the study sample. One-way ANOVA and independent samples t-test analysis were used to test the differences of different demographic characteristics in the quality of life of the urban older adults with comorbidity, and AMOS 28.0 was used to construct a social embedding structure model of the quality of life of the urban older adults with comorbidity.

The perceived quality of life among urban older adults with comorbidity was rated at a moderate level. In addition, there were significant differences in the quality of life of urban older adults with comorbidity with different genders, ages, marital status, education level, work status, and retirement status. Social support (β=0.10, P<0.001) and social resources (β=0.20, P<0.001) demonstrated positive effects on the quality of life of urban older adults with comorbidity.

Self-reported quality of life are generally higher among urban older adults with comorbidity. Social scenario has a positive effect on social support, and the quality of life of the urban older adults with comorbidity is indirectly improved by strengthening social support. At the same time, social resources not only directly contribute to the quality of life, but also indirectly have a positive impact through social support paths.

To investigate the impact of community environment on the trajectory of cognitive function among older adults in China, providing a reference for promoting the construction of age-friendly communities and actively addressing aging.

Based on the data from the China Longitudinal Aging Social Survey from 2014 to 2020, 4 269 elderly individuals were selected. A group-based trajectory model was applied to construct trajectories of cognitive function changes, and a multinomial unordered logistic regression model was used to analyze the relationship between community environment and trajectories of cognitive function.

Three groups of cognitive function trajectories were identified: “Low Decline Group” (21.06%), “Moderate Stable Group” (66.19%), and “High Stable Group” (12.75%). Taking the “low decline group” as the reference group, after controlling for individual basic characteristics and health characteristics, it was found that the higher the community environment score of the elderly, the more their trajectory tended to be in the “High Stable Group” (OR=1.10, 95%CI: 0.93-1.30; OR=1.41, 95%CI: 1.21-1.66), indicating that the community environment has a protective effect on the cognitive decline of the elderly. Stratified analysis showed that compared to females and the elderly living alone, the improvement of the community environment is more beneficial for males and the non-living alone elderly to slow down their cognitive decline.

There is heterogeneity in the cognitive function trajectories of the elderly, and the community environment affects the attribution of cognitive function trajectories. Optimizing the community environment for males and non-lonely elderly can significantly protect their cognitive function.