To analyze the characteristics of envelope protein (envelope, E) gene in dengue fever cases in Hubei Province from 2014 to 2023 and to trace the biological origin.

Serum samples of suspected dengue fever cases were collected, and nucleic acid detection and serum typing were carried out. Nucleic acid positive samples were amplified by RT-PCR and sequenced, and the gene sequences were obtained and analyzed by MEGA11.0 software.

A total of 78 dengue nucleic acid positive cases were detected in Hubei Province from 2014 to 2023, and the E gene sequence of dengue virus (DENV) was successfully obtained in 44 samples, including 39 cases of serum type Ⅰ (DENV-Ⅰ) and 5 cases of serum typeⅡ (DENV-Ⅱ). Evolutionary analysis showed that 37 cases of DENV-Ⅰ were of gene type Ⅰ (G Ⅰ), distributed in three different branches, mainly close to the epidemic strains of Southeast Asian countries such as Guangzhou, Singapore, Indonesia, Thailand, and Myanmar, 2 cases were of gene type Ⅴ (GⅤ) were close to the epidemic strains of Guangzhou and India, and 5 cases of DENV-Ⅱ were of mixed type (Cosmopolitan type).

There was multitype dengue virus infection in Hubei Province from 2014 to 2023. The main epidemic strain was GⅠ subtype of DENV-Ⅰ, which was closely related to Guangzhou, China, and Southeast Asian countries. It is suggested that Hubei Province strengthen the prevention and control of cross-provincial and cross-border transmission of dengue fever and guard against the occurrence of severe dengue and death cases.

To investigate the positive rate of HBsAg and HBsAb in the population born during 2004 and 2022 in Jiangxi Province.

By using stratified cluster sampling method, venous blood samples were collected from 8 126 people born after 2004 from 2016 to 2022 in 11 districts and cities of Jiangxi Province, and the sera were separated. HBsAg and HBsAb were detected by Elisa method, and the positive rate was calculated. The chi-square test was used to analyze the difference of positive rate among different groups.

The positive rates of HBsAg and HBsAb among people born during 2004 and 2022 in Jiangxi Province were 1.40% and 74.92%, respectively. The positive rates of HBsAg in males and females were 1.14% and 1.80%, respectively (χ²=6.18, P=0.013) and the positive rates of HBsAb were 75.55% and 74.00%, respectively (χ²=2.51, P=0.113). The positive rates of HBsAg were 1.17%, 1.31%, 2.14%, and 3.12% (χ²=13.49, P=0.004) at the age of 4, 9, 14, and 15 to 18 years, respectively, and the positive rates of HBsAb were 85.65%, 60.26%, 50.04%, and 46.42%, respectively (χ²=9.90, P <0.001). The positive rates of HBsAg and HBsAb in urban and rural areas were 1.62% and 1.14% (χ²=11.99, P=0.001), 74.18%and 75.81%, respectively (χ²=2.83, P=0.095). The positive rates of HBsAg and HBsAb in people born before and after 2012 were 2.18% and 1.14%, 52.18%, and 82.28%, respectively (χ²=7.64, P < 0.001). The positive rate of HBsAg in 11 districts and cities was between 0.45% and 2.79% (χ²=37.18, P < 0.001), and the positive rate of HBsAb was between 69.88% and 84.30% (χ²=65.61, P < 0.001).

The positive rate of HBsAg among the people born during 2004 and 2022 in Jiangxi Province is low, and the prevention and control of hepatitis B is effective. There are population and regional differences in the prevalence of HBsAg and HBsAb. Targeted measures should be taken to further improve the effect of prevention and control.

To describe the characteristics of commercial sexual behavior of HIV-infected men who have sex with men (MSM) and analyze its influencing factors.

An anonymous questionnaire survey was conducted through the recruitment of HIV-infected MSM by two social organizations in Chongqing. The χ² test was used to compare the commercial sexual behavior of HIV infected with MSM with different social demographic characteristics, mental health status, and sexual behavioral characteristics. Multivariate Logistic regression model was used to analyze the influencing factors of commercial behavior.

A total of 1 501 cases of HIV infection with MSM were investigated in this study. In total 12.50% of the respondents reported same-sex commercial sex in the past six months, of which 28.34% reported unprotected commercial sex behavior. The results of multivariate Logistic regression showed HIV-infected MSM with marital status as marriage/cohabitation (OR=2.345, 95%CI: 1.442-3.814), working status as part-time and others (OR=6.522, 95%CI: 3.575-11.900), monthly income exceeding 5 000 yuan (OR=7.890, 95%CI: 4.254-14.634), age of first sexual intercourse less than 18 years old (OR=1.818,95%CI: 1.014-3.260), using same-sex dating software (using one: OR=2.984, 95%CI: 1.477-6.030; using two: OR=3.683,95%CI: 1.650-8.221; using 3 and above: OR=21.777, 95%CI: 8.349-56.804), drinking (OR=2.528, 95%CI: 1.568-4.074), and using Rush (OR=3.114, 95%CI: 1.988-4.877) were more likely to have commercial sexual behavior (P < 0.05).

The commercial sexual behavior of HIV infection with MSM is common in Chongqing, and the proportion of commercial sexual behavior without condom is high. Comprehensive measures should be taken to intervene the drinking, Rush use, and commercial sexual behavior of this population in order to reduce the further spread of HIV/STIs.

To understand the distribution, serotype, and drug resistance of Salmonella in domestic sewage in Jining city, and to analyze the whole genome sequencing so as to provide scientific basis for the prevention and control of Salmonella.

From February to March in 2023, two samples of sewage from the intake of 3 urban sewage plants with 500 ml each were collected continuously for 7 weeks. A total of 42 sewage samples were collected for culture, isolation, and identification of Salmonella. The isolated Salmonella was tested for serum agglutination test and fluorescent quantitative PCR serotyping. In total 41 strains of Salmonella were tested for minimum inhibitory concentration (MIC) of 17 drugs in 9 classes by microbroth dilution method, followed by genome sequencing and phylogenetic analysis.

The isolation rate of Salmonella from domestic sewage in Jining city was high. Among 24 positive samples, 41 strains of Salmonella were isolated, belonging to 9 serotypes, mainly S. Agona and S. Kentucky. Liying and Jidong sewage treatment plants had their own unique serotypes. Only one strain of S. Give was not resistant, and the other 40 strains of Salmonella were multi-drug resistant. The resistance rates to sulfamethoxazole, tetracycline, ampicillin, and streptomycin were more than 90%, and the resistance rate to ampicillin/sulbactam was more than 80%. There were 19 kinds of drug resistance spectra, of which SXT-TET-STR-AMP-AMS ranked the first place. The resistance spectra of different serotypes of Salmonella were different. S. Kentucky, S. Gold Coast, and S. Infant were multi-drug resistant seriously. The resistance spectrum of the same serotype was also inconsistent in different sewage plants, and the resistance was the most serious in Liying sewage plant. The CARD database was used to predict drug resistance genes, mainly predicting 18 drug resistance genes in 8 types of drug resistance families, and the drug resistance phenotypes and drug resistance genes were almost all consistent. The ST types of different serotypes of Salmonella were different, and ST13 S. Agona was dominant. The phylogenetic tree was divided into 6 clusters, and strains in different sewage plants were in different branches, indicating that their genomes were different and had different sources.

There are various serotypes of salmonella in domestic sewage in Jining city, suggesting that they might have exist and spread in the population. Almost all strains are multi-drug resistant bacteria, carrying resistance genes extensively. Some types of strains live persistently and distribute across regions, leading to the potential risk to population. It is necessary to carry out continuous monitor, and scientific prevention and control measures need to be formulated to control the epidemic of salmonella and the aggravation of drug resistance.

To analyze the relationship between stress resistance, symptom interference, and cancer-related fatigue in cancer patients undergoing radiotherapy and chemotherapy.

A total of 336 cancer patients undergoing radiotherapy and chemotherapy in the First Affiliated Hospital of Hebei North University from October 2020 to October 2021 were selected by convenient sampling. The related data were collected by general condition questionnaire, cancer fatigue scale (CFS), Chinese version memory symptom assessment scale (MSAS-Ch), and stress resistance scale (CD-RICS).

The scores of cancer-related fatigues, stress resistance, and symptom disturbance were 30.55 ±8.26, 62.14 ±14.04, and 1.11 ±0.38, respectively. The level of stress resistance played an intermediary role in symptom interference and cancer-related fatigue, accounting for 26.26% of the total effect. The level of stress resistance had a regulatory effect between symptom interference and cancer-related fatigue (β = 0.102, ΔR²=0.382, P < 0.05).

The level of stress resistance plays a mediating effect and regulation between symptom interference and cancer-related fatigue. Encouraging patients to strengthen the level of stress resistance, strengthen positive psychological construction, and weaken the level of symptom interference is helpful to reduce cancer-related fatigue.

To analyze the relationship between the proportion of omega-6/omega-3 and mortality in patients with cardiovascular disease (CVD), so as to provide reference for reducing the risk of death in patients with CVD.

The data were collected from the UK perspective cohort (UK Biobank) database. The exposure variable was the proportion of plasma omega6/omega-3, and the outcomes were all- cause deaths, CVD deaths, ischemic heart disease (IHD) deaths, and stroke deaths. Multivariate Cox proportional hazard regression model was used to analyze the relationship between omega-6/omega-3 ratio and death risk of CVD patients. Hazard ratio (HR) and 95% confidence interval (CI) were calculated. Gender, age, and other risk factors were stratified and their interaction with omega-6/omega-3 ratio was analyzed. Restricted cubic spline (RCS) was used to describe the dose-response relationship. Sensitivity analysis was used to test the robustness of the model.

A total of 35 096 participants were enrolled. Multivariate Cox proportional hazard regression model showed that all-cause deaths, CVD deaths, and ischemic heart disease deaths risk increased in CVD patients with the increase of the proportion of omega-6/omega-3. For each unit increase in omega-6/omega-3 ratio, the risk of all-cause, CVD, and IHD deaths increased by 2.1% (HR=1.021, 95%CI: 1.016-0.025), 1.9% (HR=1.019, 95%CI: 1.010-1.028), and 2.3% (HR=1.023, 95%CI:1.013-1.034), respectively. The proportion of omega-6/omega-3 was grouped according to quartile method and included in the model as a classified variable. Compared with Q1, the HR of all-cause, CVD, and IHD deaths of Q4 was 1.394 (95%CI: 1.267-1.534), 1.292 (95%CI: 1.085-1.540), and 1.528 (95%CI: 1.200-1.945), respectively, and no correlation was found between Q4 and stroke death risk (HR=0.987, 95%CI: 0.660-1.477). The results of RCS showed that the proportion of omega-6/omega3 was positively correlated with the risk of death.

The proportion of omega-6/omega-3 is a risk factor for death in patients with CVD.

To investigate the relationship between disease fear, self-efficacy, and psychological resilience after coronary artery bypass grafting (CABG) in Xinxiang of Henan province.

A total of 137 patients who underwent coronary artery bypass grafting in Xinxiang Central Hospital were selected by convenient sampling method. Fear disease progression simplified scale (FoPQ-SF) was used to investigate the status of postoperative fear of disease before discharge, 6 weeks, and 3 months after operation, and self-efficacy scale (GSES) and psychological resilience scale (CDRISC) were used to evaluate their self-efficacy and psychological resilience. Pearson method was used to analyze the correlation between GSES score, CD-RISC score, and FoP-Q-SF score, and Logistic regression model was used to analyze the influencing factors of self-efficacy and psychological resilience on disease fear progression.

The scores of FoP-Q-SF items, total score, GSES score, and CD-RISC score increased gradually before discharge, 6 weeks, and 3 months after operation, and the difference was statistically significant. Pearson correlation showed that GSES score and CD-RISC score were positively correlated with FoP-Q-SF score. Stepwise multivariate Logistic regression analysis showed that GSES score and CD-RISC score were the influencing factors of postoperative disease fear progression.

The disease fear status of patients after coronary artery bypass grafting in Xinxiang area of Henan Province is at a high level, and self-efficacy and psychological resilience are closely related to it, which are also the influencing factors of postoperative disease fear progression.

To explore whether there is any causal association between ketone bodies and cognitive performance employing the bidirectional and two-sample Mendelian randomization (MR) method.

Single nucleotide polymorphisms (SNPs) from the genome-wide association studies summary data from Europe were used as instrumental variables. The inverse-variance weighted method was used as the primary method to estimate the causal effect of ketone bodies instrumental variables with cognitive performance, and pathway and functional enrichment analyses were performed to predict potential mechanisms. Significance was ascertained with the use of Bonferroni-corrected significance thresholds (P=0.017).

Two-sample MR analysis showed that acetoacetate (β=0.075, 95% CI: 0.002-0.148, P=0.045) and β-hydroxybutyrate (β=0.082, 95% CI: 0.039-0.126, P=2.36×10^-4) were causally associated with cognitive performance. However, the association of acetoacetate was no longer significant after Bonferroni-corrected. In addition, no link between acetone (β=0.041, 95% CI: -0.018-0.099, P=0.175) and cognitive performance was discovered. Pathway and functional enrichment analyses suggest that the mechanism of β-hydroxybutyrate effect on cognitive performance may be through a variety of ways, such as cholesterol metabolism and insulin resistance. In reverse MR Analysis, we found no causal effects of cognitive performance on ketone bodies.

Our findings support a causal relationship between β-hydroxybutyrate levels and better cognitive performance.

To explore the effect of serum lipid levels on the risk of developing cognitive decline in middle-aged and older adults.

Data were obtained from the China Retirement and Follow-up Survey Cohort(CHARLS) cohort from 2011-2018, and middle-aged and older adults aged ≥45 years were selected for cognitive assessment of middle-aged and older adults who were followed up in 2018 by using the Chinese version of the Brief Mental Scale(MMSE), and multifactorial logistic regression was utilized to analyze middle-aged and older adult populations for serum total cholesterol(TC), triglycerides(TG), high-density lipoprotein cholesterol(HDL), low-density lipoprotein cholesterol(LDL) and lipid composite index(LCI) in relation to cognitive function.

A total of 6 556 participants with a mean age of 57.03 ± 8.03 years were included. After adjusting for multiple confounders, compared with the lowest quartile group(Q1), highest quartile group(Q4) of TG(OR=1.229, 95% CI:1.036-1.456), LDL(OR=1.221, 95% CI:1.034-1.443), and LCI(OR=1.282, 95% CI:1.081-1.522) were positively associated with the cognitive decline. The risk of developing cognitive decline was positively associated with the risk of cognitive decline, HDL(OR=0.765, 95% CI:0.646-0.906) was negatively associated with the risk of cognitive decline, and TC(OR=1.010, 95% CI:0.855-1.193) was not associated with the risk of cognitive dysfunction, and the difference was not statistically significant.

TG, LDL and LCI are risk factors for the risk of cognitive decline in middle-aged and elderly people, and HDL are protective factors for the risk of cognitive decline in middle-aged and elderly people.