To explore the status and influencing factors of health poverty among the elderly in rural areas of Sichuan in the post poverty alleviation era.

By using the method of multi-stage sampling, a questionnaire survey was conducted among 606 rural elderly persons in Sichuan from July to August, 2022. Chi-square test and multivariate logistic regression analysis were used to explore the influencing factors of health poverty.

Among the 606 persons, 167 (27.6%) were in health poverty.The results of multivariate analysis showed that age(OR=1.655, 95%CI: 1.250-2.191), number of chronic diseases (OR=1.864, 95%CI: 1.510-2.301) and physical function limitation (OR=1.890, 95%CI: 1.353-2.640) were the risk factors, while the number of daughters (OR=0.739, 95%CI:0.593-0.920) and labor intensity in the past year (OR=0.498, 95%CI: 0.343-0.722) were protective factors for health poverty in the rural elderly.

The rural elderly in Sichuan still have a high risk of health poverty, especially the elderly who are aged, suffer from multiple chronic diseases, have serious disability, have little labor intensity, and have few daughters are the high-risk groups of health poverty, which needs to be paid attention to.

To understand the impact and effect value of basic medical insurance on the health status of children in China, and to propose corresponding countermeasures and suggestions.

Based on the data from the Chinese Family Panel Studies in 2016 and 2020, this study examined the extent to which basic medical insurance impacts children’s health using the Propensity-Score-Matching and Difference-in-Difference models.

The findings indicated that basic medical insurance could promote the health status of children by 4.9%(β=0.049,SE=0.028). However, the mediating effects of reducing health costs and improving health service utilization were found to be insignificant. Moreover, the study revealed that the health status of preschool children, girls, rural areas, western regions, and low-income families can be improved by 9.1%(β=0.091,SE=0.038), 7.8%(β=0.078,SE=0.043), 9.3%(β=0.093,SE=0.041), 12.0%(β=0.120,SE=0.051), and 9.3%(β=0.093,SE=0.055) respectively.

This study concludes that basic medical insurance plays a positive role in enhancing children’s health. Therefore, it is crucial to continuously strengthen the basic medical insurance system and reinforce the multi-tiered healthcare security framework. Attention should also be given to ensuring fairness for girls and children in rural and western areas, in order to fully protect children’s health.

To explore the relationship between lifestyle and depression symptoms among college students.

A total of 21 143 college students from a private university in Shaanxi province were selected to conduct an online questionnaire survey, including social demographic characteristics, lifestyle and depression symptoms. GAMMA regression and Logistic regression models were used to analyze the association between five lifestyle and unhealthy lifestyle scores and depression symptoms in college students.

The depression symptom score of college students was 29(22,36), and the prevalence of depression symptoms was 15.57%. The prevalence of smoking, drinking, unhealthy diet, lack of physical activity, low body weight or obesity were 17.98%, 24.24%, 83.45%, 80.11% and 46.63%, respectively. The five unhealthy lifestyles were significantly correlated with depression symptoms of college students (all P<0.05). Regression analysis showed that, compared with the unhealthy lifestyle scores of 0-1, the score of depression symptoms increased in the group with 3, 4 and 5 score of unhealthy lifestyle, with β(95%CI) values of 0.57(0.23-0.92), 1.91(1.48-2.34) and 2.47(1.79-3.15), respectively. Compared with the unhealthy lifestyle scores of 0-1, the risk of depression symptoms was increased in unhealthy lifestyle scores of 2, 3, 4 and 5, and the odds ratio value (95%CI) were 1.19(1.02-1.40), 1.41(1.21-1.65), 1.86(1.56-2.21) and 2.18(1.71-2.78), respectively.

Unhealthy lifestyle is related to the increased risk of depression symptoms in college students. The more types of unhealthy lifestyle, the higher the score of depression symptoms and the higher the risk of depression symptoms.

To explore the relationship and mechanism of stress perception and loneliness among emotional intelligence and life satisfaction among master’s students, and to provide reference basis for promoting the mental health development of graduate students.

A total of 728 graduate students from 6 universities were selected by stratified cluster random sampling and investigated by emotional intelligence, loneliness, stress perception and life satisfaction scale. SPSS 25.0 was used to conduct independent sample t-tests, analysis of ANOVA, and Pearson correlation analysis on the data. A structural equation model was bulit by using AMOS 26.0 software and evaluated.

Emotional intelligence was positively correlated with life satisfaction (r=0.423, P<0.01). There was a negative correlation between perceived stress and loneliness, life satisfaction, and emotional intelligence (r=-0.519, -0.440, -0.536, -0.505,P<0.01). There was a positive correlation between perceived stress and loneliness (r=0.568, P<0.01). Emotional intelligence can not only directly predict life satisfaction, but also indirectly through stress perception and loneliness. The mediation effect value of stress perception was 0.227 accounting for 42.5% of the total effect,and the mediation effect value of loneliness was 0.026, accounting for 4.9% of the total effect. The effect value of emotional intelligence and life satisfaction was 0.029, accounting for 5.4% of the total indirect effect.

Emotional intelligence can affect the life satisfaction of master’s students through the mediating or chain mediating effect of stress perception and loneliness, which provides a theoretical basis for improving the quality of life and mental health of master’s students.

To provide a theoretical basis for optimizing and improving the culture medium of Yersinia pestis, the effects of different species and different concentrations of animal blood on the growth of Yersinia pestis were investigated.

Ten kinds of animal blood including rat blood, rabbit blood and guinea pig blood at four concentrations of 0.1 %, 0.5 %, 1 % and 2 % were used as blood stimulators to culture Yersinia pestis in the medium. The culture results were observed and the corresponding statistical analysis was carried out.

The effect of 10 kinds of animal blood on the number of Yersinia pestis colonies at 1 % concentration was different (P=0.015 < 0.05), and pig blood was better than chicken blood (P=0.026 < 0.05). The effect of four concentrations of pig blood on the number of Yersinia pestis colonies was statistically significant (P=0.037 < 0.05), and it was better than the blank at 1 % concentration (P=0.002 < 0.05). The effects of ten kinds of animal blood at four concentrations on the length of Yersinia pestis were statistically significant (P<0.001). The difference in the effect of four concentrations of blood on the length of Yersinia pestis in ten animals was also statistically significant (P<0.001).

When observing the number of colonies, the optimal choice is 1% pig blood. In addition, low concentration and easy-to-obtain blood stimulants can be selected as far as possible. To cultivate a better length of Yersinia pestis, the optimal choice is 0.1% rabbit blood, which can achieve the desired experimental results and save costs. In addition, using the Herxheimer’s sensitive medium with only blood stimulator to culture Yersinia pestis, the characteristics of the strain will not undergo L-type transformation, and the overall characteristics of the strain are stable, which is of great significance for the stable passage and preservation of the strain.

To explore the Rap1/PI3K/AKT signaling pathway in inducing apoptosis of mouse spermatocytes (GC-2 spd) induced by tris (1,3-dichloro-2-propyl) phosphate (TDCIPP)the role of death.

GC-2 spd cells were treated with 0, 15, 30, and 60 μmol/L TDCIPP for 24 hours.Using flow cytometry to detect cell expression levels, Western blotting to examine the expression levels of Rap1, PI3K, AKT, phosphorylated AKT (p-AKT) proteins, as well as related proteins cleared PARP and cleared Caspase-3 in cells, and apoptosis-related proteins cleaved PARP and cleaved Caspase-3 in the cells. ESI09 was used to inhibit the expression of Rap1 protein in GC-2 spd cells, followed by exposure to TDCIPP. Western blotting and flow cytometry were used to respectively assess the protein levels of PI3K, AKT, p-AKT, cleaved PARP, and cleaved Caspase-3, as well as the level of cell apoptosis.

Compared to the control group, the cell apoptosis and expression of cleaved PARP and cleaved Caspase-3 proteins were significantly increased in the TDCIPP-exposed cells (P<0.01). The expression level of Rap1 was significantly increased (P<0.01), while the expression levels of PI3K, AKT, and p-AKT were decreased (P<0.01). Compared to the TDCIPP-exposed group, the expression of Rap1 was down-regulated (P<0.01), while the expression levels of PI3K, AKT, and p-AKT proteins were up-regulated (P<0.05) in the cells co-treated with ESI09 and TDCIPP. Meanwhile, the expression levels of cleaved PARP and cleaved Caspase-3 in the cells, as well as the level of cell apoptosis, were significantly reduced (P<0.05).

TDCIPP induces overexpression of Rap1 protein and obstructs PI3K/AKT signaling transduction, eventually leading to apoptosis of mouse spermatocytes.

To investigate the expression and clinical significance of protein-O-fucosyltransferase 1 in non-small cell lung cancer (NSCLC), so as to provide a reference for the diagnosis and treatment of NSCLC.

The expression of POFUT1in NSCLC was determined by TCGA, UALCAN database and immunohistochemistry. Based on the clinical data form TCGA database were extracted for survival analysis by Kaplan-Meier method, and analysis of the effect of POFUT1 mRNA expression level on prognosis of patients with NSCLC.

POFUT1 was highly expression in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) tissues (all P<0.05). The expression level of POFUT1 mRNA in LUAD was correlated with clinical stage (P<0.001) and that in LUSC was correlated with tumor size (P<0.001). COX regression analysis showed that high mRNA expression of POFUT1 (P=0.012), tumor size, clinical stage and lymph node metastasis were associated with the prognosis of LUAD patients (all P<0.001), and clinical stage III-IV was an independent risk factor for LUAD patients. Tumor size (P=0.001), clinical stage III-IV (P=0.006) and high expression of POFUT1 mRNA (P=0.049) were associated with the prognosis of LUSC patients, and tumor size greater than 5cm and high expression of POFUT1 were independent risk factors for LUSC patients. The survival analysis result showed that the survival rate of patients with high expression of POFUT1 was significantly lower than that of patients with low expression.

POFUT1 is highly expressed in NSCLC tissues, and high POFUT1 expression is correlated with poor prognosis in patients. POFUT1 can serve as a biomarker for prognostic evaluation of NSCLC.

To examine the correlation between body composition and insulin resistance (IR) within the general population of different genders.

The data for this study were sourced from the Hefei area data of the Chinese Residents Cardiovascular Disease and Risk Factors Monitoring Project conducted in 2021, with a total of 1 144 subjects being included,including 568 males and 576 females. The ratio of triglycerides to high-density lipoprotein cholesterol (TG/HDL-C) was used as an alternative indicator of insulin resistance. A chi-square test was employed to analyze the association between body composition metrics—such as body mass index (BMI), percent body fat (PBF), percent body muscle (PBM), visceral fat area (VFG), and waist circumference (WC)—and the TG/HDL-C ratio. Logistic regression analysis was used to identify the factors influencing body composition in individuals with a TG/HDL-C ratio exceeding the tangent point value. The dose-response relationship between body components and the TG/HDL-C ratio was explored using restricted cubic splines, a statistical method that allows for the examination of nonlinear relationships.

The positive detection rate of IR (the rate of TG/HDL-C above the cut point value) was 15.85% in men and 4.69% in women.After adjusting for confounding factors, logistic regression analysis showed that compared with the control group, the risk of IR in the male population was 5.58 times in the obese group (OR=5.58, 95%CI:1.98-15.72), PBF was 7.90 times in the third quartile (OR=7.90, 95%CI:1.68-37.15), the fourth quartile of PBF was 8.20 times (OR=8.20, 95%CI:1.75-38.40), and the third quartile of PBM was 0.27 times (OR=0.27, 95%CI:0.10-0.71), the PBM was 0.09 times in the fourth quartile group (OR=0.09, 95%CI:0.02-0.44), 3.11 times in the group with high VFG (OR=3.11, 95%CI:1.45-6.68), and 4.28 times in the group with higher VFG (OR=4.28, 95%CI:1.45-12.64). Abdominal obesity was 2.21 times (OR=2.21, 95%CI:1.08-4.56). In the female population, the risk of IR was 6.61 times in the obese group (OR=6.61, 95%CI:1.08-65.66) and 8.52 times in the higher VFG group (OR=8.52, 95%CI: 1.90-70.86) (P<0.05). The limited cubic spline showed that BMI, PBF, PBM, VFG, WC had dose-response relationship and approximate linear relationship with TG/HDL-C.

The body composition of community residents is closely associated with the risk of insulin resistance. Individuals who are obese or have high levels of visceral fat are at greater risk for insulin resistance. To mitigate this risk, it is imperative to reinforce lifestyle interventions that prioritize maintaining a higher percentage of muscle mass.

To investigate the influenza vaccination willingness and influencing factors among elderly patients with chronic diseases in Chongqing, providing a scientific basis for the development of targeted vaccine policies and intervention measures.

A multi-stage sampling method was employed to randomly select 1 280 elderly patients with chronic diseases in five districts of Chongqing for an investigation into their influenza vaccination willingness. Univariate analysis was performed using the t-test and chi-square test, and binary logistic regression analysis and the standardized score were used to analyze the influencing factors of influenza vaccination willingness among elderly patients with chronic diseases.

Of the 1 211 eligible survey respondents, only 13.1% of elderly patients with chronic diseases had received the influenza vaccination during the 2022-2023 influenza season, and 56.3% were willing to receive the influenza vaccination in the 2023-2024 influenza season. The willingness to receive influenza vaccination among elderly patients with chronic diseases was higher among ethnic minorities (OR=3.49, 95%CI:1.21-10.02), households or unemployed individuals (OR=2.53, 95%CI:1.05-6.09), individuals with an average monthly household income per capita exceeding 5 000 RMB (OR=2.86, 95%CI:1.33-6.13), those with qualified knowledge of influenza vaccination (OR=1.38, 95%CI:1.02-1.87), and under the policy of free vaccination (OR=56.97, 95% CI:24.38-133.09). The behavioral and social drivers (BeSD) survey indicated that the belief in the effectiveness and safety of the influenza vaccine and fear of infecting friends or family members were the primary driving factors of influenza vaccine uptake, while the perception of the high costs of influenza vaccination was the most significant barrier.

The influenza vaccination willingness among elderly patients with chronic diseases in Chongqing needs further improvement. Governmental authorities, healthcare institutions, and communities should enhance the dissemination of knowledge about influenza vaccines, enhance social support, implement subsidies or free vaccination policies for priority populations, and encourage proactive influenza vaccine uptake among elderly patients with chronic diseases.

To analyse the current situation and trends of wasting and overweight obesity among Han Chinese college students from 2000 to 2019, and to provide a basis for the prevention of wasting and overweight obesity among college students.

The body mass index data of 72 983 Han Chinese college students were selected from five national surveys on students’ physical fitness and health from 2000 to 2019, and the “height-standard weight method” used in the 1985 national survey on physical fitness and health was used to screen for emaciation and overweight-obesity, and the detection rate, increase and annual growth rate of emaciation and overweight-obesity were calculated for college students by gender and age. The detection rate, increase rate and annual growth rate of college students were calculated by gender and age. Statistical analyses were performed using one-way ANOVA, sample-weighted linear regression, χ² test and χ² trend test.

From 2000 to 2019, the BMI of Chinese Han college students all showed a continuous increasing trend, with different growth rates for male and female students in each age group (except for the female group, the P-trend value for male students in each age group was <0.05). Both male and female students' wasting detection rates showed a decreasing trend from 2014-2019.From 2000 to 2019, the overweight and obesity rate of Chinese Han college students continued to increase, and the overweight and obesity rate of male students was higher than that of female students.From 2000 to 2019, the wasting of Chinese Han college students aged 19 to 22 years old The annual growth rate of overweight and obesity of male students has been decreasing, and the annual growth rate of overweight and obesity of male students has been increasing, and the difference in the increase of the annual growth rate of overweight and obesity of male students in each age group is relatively small. 2010-2019, the annual growth rate of overweight and obesity of female students began to increase gradually, and the increase was the largest in 2014-2019, and the increase was the most obvious The largest increase in the annual growth rate of overweight obesity among female students was observed in the age group of 22 years.

At 2019, the trend of the growth of college students' wasting rate has been alleviated, but the overweight and obesity rate is in a continuous growth trend, the relevant government departments should formulate targeted physical health policies to prevent and alleviate the problem of college students’ overweight and obesity.