To explore the mediating effect of physical function and nutritional status between sleep quality and frailty in rural elderly people based on Gobbens frailty integration model.

From July to September 2023, multi-stage stratified cluster random sampling was used to select the elderly aged ≥60 years old in rural areas of Xinjiang, and questionnaire survey and body measurement were used to investigate the study. AMOS 28.0 software was used to establish the structural equation model (SEM) and test the mediating effect.

(1) Frailty scores of the rural elderly were 40.00 (34.00,46.00), sleep quality scores were 7.00 (5.00,9.00), physical function scores were 9.00 (7.00,10.00), nutrition scores were 13.00 (12.00,14.00); (2) The score of sleep quality of the rural elderly was positively correlated with the score of frailty, and negatively correlated with the score of physical function and nutritional status (r=0.405, -0.222, -0.125, P<0.001). The score of physical function was negatively correlated with the score of frailty, and positively correlated with the score of nutritional status (r= -0.220, 0.216, P<0.001). Frailty score was positively correlated with nutritional status score (r= -0.071, P=0.024); (3) Physical function and nutritional status played a mediating role between sleep quality and frailty, accounting for 15.76% of the total effect.

Physical function status and nutritional status play an intermediary role in the sleep quality and frailty of the rural elderly. Through early identification of sleep problems, physical function status and nutritional status can be improved, delaying or even reversing the occurrence of frailty.

To analyze the levels of acrylamide in 2-5 years old children of three regions in China and assess the exposure risk.

The survey was conducted on 31 healthy children aged 2-5 years old in Guangxi Zhuang Autonomous Region, Heilongjiang and Sichuan Province. We collected the whole food consumed by the children during the survey period using the dual meal method, analyzed the acrylamide exposure level. Then the margin of exposure (MOE) assessment was used to evaluate the carcinogenic and non-carcinogenic risks by using BMDL10 (0.31 mg/(kg·bw·d)) and NOAEL (0.2 mg/(kg·bw·d)) as toxicity reference points.

This survey investigated 13 girls and 18 boys in three regions of China, aged between 26 to 58 months, with the weight range of 11.0 to 20.0 kg and the height range of 87.0 to 115.1 cm. A total of 187 food samples were collected, including 96 mixed diet samples, 61 liquid dairy product samples, and 30 infant formula samples. The average levels of acrylamide from the three types of samples were 9.54 μg/kg, 0.80 μg/kg, and 1.03 μg/kg. The average exposure levels were 0.624 μg/(kg bw·d), 0.04 μg/(kg bw·d), and 0.04 μg/(kg bw·d).The high exposure levels of acrylamide (P₉₅) increased to 2.974 μg/(kg bw·d), 0.234 μg/(kg bw·d), and 0.13 μg/(kg bw·d). The number of average MOE and high MOE (P₉₅) for non-carcinogenic risk of acrylamide are 299 and 67, while the number of average MOE and high MOE (P₉₅) for carcinogenic risk are 463 and 104.

The number of MOE for both non carcinogenic and carcinogenic risks of acrylamide in the diet of 31 young children are less than 10 000, and the number of MOE for high non carcinogenic risks is less than 100. Therefore, it is necessary to pay special attention to the health risks caused by dietary intake of acrylamide in young children.

To explore the effects of different sequential strength and endurance training on body composition and cardiorespiratory endurance of primary school students, and to provide theoretical basis for scientific guidance of primary school students’ fitness and improvement of children’s physical health. Subjects 60 boys in grade 5 of a primary school in Beijing (age: 10.22 y±0.73 y; height: 150.23 cm±9.63 cm; weight:45.71 kg±8.09 kg; BMI: 20.25 kg/m²±4.77 kg/m²).

The subjects were divided into three groups: simple endurance group (n=20), RE group (n=20) and ER group (n=20). Three groups of subjects were trained for 12 weeks, 3 times a week. Maximum oxygen uptake, body composition, lung function and other indicators were tested before and after intervention.One-way ANOVA and Paired Sample T-Test were used for statistical analysis.

After 12 weeks of exercise training intervention, there was no significant difference in the growth rate ofthe weight of the subjects between the three groups (Egroup: 2.78±7.23; REgroup: 4.00±7.20; ER groupin the same period: 3.47±7.94)(P>0.05).Compared with the simple endurance group, the percentage of physical fitness (Egroup: 10.69±3.34; RE group: 16.38±6.34; ER group: 23.15±10.30) was significantly decreased (P<0.01), and the ER group was better than the RE group (P<0.05). The muscle growth rate (Egroup: 6.84 ± 2.91; RE group: 12.58 ± 3.68; the ER group: 10.63±2.91) in the same period group was higher than the simple endurance group (P<0.01), and the RE group was higher than the ER group (P<0.05).The growth rate of vital capacity (Egroup: 12.71±4.64; RE group: 20.35±4.22; the ER group: 17.76±5.51) in the same period group was higher than that in the endurance group (P>0.05).There was no significant difference in the growth rate of vital capacity body mass index between the three groups (E group: 9.43±8.23; RE group: 16.55±7.96; the same period ER group: 13.44±10.26) (P>0.05).The maximum oxygen uptake growth rate (Egroup: 7.06±2.55; RE group: 14.10±4.12; the ER group: 11.26±4.04) in the same period group was higher than the endurance group (P>0.05).

Concurrent training is superior to simple endurance training in improving children’s body composition and cardiopulmonary endurance. The endurance-strength training sequence can effectively reduce the body fat content, the training sequence of strength-endurance can effectively increase the content of skeletal muscle and the improvement of children’s cardiopulmonary function is not related to the training sequence.

To analyze the associated factors affecting depressive symptoms in middle school students and to construct a risk prediction line graph model to assess the risk of developing depressive symptoms in middle school students.

A total of 1 088 middle school students were selected to participate in the monitoring and intervention project of common diseases and health influencing factors in Zhongmu County, Zhengzhou City, using the information collected from the questionnaires. A multivariate logistic regression analysis was employed to identify the factors that influenced the occurrence of depressive symptoms, and a column graph was established to predict the risk of depressive symptoms in middle school students.

The prevalence of depressive symptoms among middle school students reached 28.50%. Female students (OR=2.051,95% CI：1.475-2.851), living in school (OR=1.496,95% CI：1.069-2.093), abnormal family (OR=1.615,95% CI：1.013-2.530), beaten and scolded by parents within one month (OR=3.516,95% CI：2.159-5.726), smoking (OR=2.044,95CI：1.298-3.219), serious injury within one year (OR=2.576,95CI：1.225-5.417), bullying(OR=3.276,95CI：1.113-9.643), internet addiction (OR=7.421,95CI：4.624-11.909) can increase the risk of depressive symptoms in middle school students. Average daily fruit intake in a week (<1time/day: OR=0.513,95% CI：0.306-0.863; ≥1time/day: OR=0.500,95% CI：0.290-0.863), reduce the risk of depressive symptoms in middle school students. The Nomogram model based on the above factors had good differentiation (AUC=0.752) and accuracy (Hosmer-Lemeshow test result χ²=3.657, P>0.05).

Middle school students who are girls, live in school, abnormal families, beaten by parents within one month, seriously hurt within one year, smoking, bullying and Internet addiction are more likely to have depressive symptoms. The column-chart can be used to predict the risk of depression symptoms in middle school students effectively, so as to take timely intervention measures for high-risk groups.

To translate the Chinese version of Personal Experience Scale (PECK-SF) into Chinese and test its reliability and validity in primary and secondary school students in China.

The Chinese version of PECK-SF was used to investigate the bullying victimization of 10 250 primary and secondary school students over 9 years old, and exploratory factor analysis and confirmatory factor analysis were conducted respectively. The Center of Epidemiological Studies Depression Scale for Children (CES-DC) and the School Connectedness Scale (SCS) were used as criterion scales.

Item analysis showed that the scores of all items in Chinese version of PECK-SF were significantly positively correlated with the total score of the scale (r=0.548-0.704, P<0.001). Exploratory factor analysis showed that the 4-factor cumulative variance explanation rate was 64.709%. The results of confirmatory factor analysis showed that the scale fitting index was good (RMSEA=0.066, RMR=0.009, GFI=0.952, AGFI=0.929, CFI=0.941). The Chinese version of PECK-SF total scale and score by each dimension were positively correlated with CES-DC scores (r=0.273-0.492, P<0.001), and negatively correlated with SCS scores (r= -0.198——0.347, P<0.001). The Cronbach’s α coefficient of this scale is 0.879, and the split-half reliability is 0.764.

The Chinese version of PECK-SF has good reliability and validity and can be used as a bullying victimization assessment tool for primary and secondary school students over 9 years old.

To develop a questionnaire of menstrual health literacy for adolescent women and evaluate its reliability and validity to provide a scientific basis for evaluating the level of adolescent menstrual health literacy.

The initial questionnaire was compiled by combining literature search, semi-structured interview and Delphi expert consultation, and the test questionnaire was formed after revising items through item analysis and exploratory factor analysis.1545 middle and high school students were selected by convenient sampling method to test the reliability and validity of the questionnaire.

The adolescent health literacy questionnaire consisted of 4 primary indicators, 15 secondary indicators, 41 tertiary indicators and 60 measurement questions. Cronbach’s α coefficient of the total questionnaire was 0.890, and the broken half reliability was 0.853, indicating good reliability of the questionnaire.Most of the three indexes of the questionnaire were more than 85% suitable for inclusion, and 95% of the measurement questions had I-CVI value >0.80, indicating good content validity.The results of confirmatory factor analysis showed that the validity of the questionnaire structure was basically acceptable.The questionnaire response validity was measured based on the hypothesis that people with higher knowledge satisfaction and healthier lifestyle had higher questionnaire scores, and the results showed that the questionnaire response validity was good.

The adolescent menstrual health literacy questionnaire developed in this study has good reliability and validity, and can be used to evaluate the level of adolescent female menstrual health literacy.

To investigate the status of drug resistance of Mycobacterium tuberculosis(M.tuberculosis) isolated from drug resistance monitoring stations in Changsha from 2021 to 2023, and to provide reference for the control effect of drug-resistant tuberculosis.

The positive specimens from designated medical institutions in Changsha were collected and sent to our hospital together with the basic information of patients for further M.tuberculosis drug susceptibility test. Drug susceptibility tests were performed on all samples by proportional method, including 8 drugs, such as RFP, INH, EMB, SM, OFX, PAS, TH, KM, etc, and the qualitative data were analyzed by χ² test.

Among the 3 037 isolates, 2 534 were fully sensitive and 503 were drug-resistant, and the total drug resistance rate was 16.56%. Among them, 316 were single drug resistant, and the drug resistance rate was 10.41%. There were 81 multi-drug resistant strains (2.67%) and 106 poly-drug resistant strains (3.49%). The drug resistance sequences of any drug were INH, SM and RFP, 9.02%, 6.98% and 3.65%, respectively. The top three of multi-drug resistant patterns were INH+RFP (0.66%), INH+RFP+SM (0.4%) and INH+RFP+SM+EMB (0.36%), respectively. The top three poly-drug resistant patterns were INH+SM (1.15%), INH+TH1321 (0.4%) and INH+EMB (0.2%), respectively. In addition, from year of 2021 to 2023, the resistance of M.tuberculosis to RFP, EMB, and TH1321 drugs in Changsha area showed a decreasing trend year by year (P<0.05), but the overall resistance rate of patients in Changsha urban area was significantly higher than that in other districts and counties.

Compared with other provinces and cities, the overall drug resistance rate of tuberculosis patients in Changsha is low, but the drug resistance rate of tuberculosis in Changsha urban area is significantly higher than that in other districts and counties, which should arouse the attention of relevant departments.

To analyze the influencing factors of the first visit of elderly people at the grassroots level in China, and further implementing graded diagnosis and treatment policies.

Based on the 2020 CLASS data, 4 444 elderly people were included, and a chi square test was used to analyze the influencing factors of their primary diagnosis behavior. A random forest model was used to rank the importance of variables with significant impact.

The elderly who living in the eastern region (OR=1.791,95% CI:1.421-2.256) and the central region (OR=1.438,95% CI:1.121-1.846), having a general self-evaluation (OR=1.869,95% CI:1.501-2.327) and self-assessment health (OR=2.342,95% CI:1.869-2.936), having two kinds of chronic diseases (OR=1.379,95% CI:1.174-1.621) and more than two kinds of chronic diseases (OR=2.648,95% CI:2.236-3.137), using health intelligent devices (OR=1.192,95% CI:1.032-1.377), using basic health services(OR=1.756,95% CI:1.527-2.020), college degree or above (OR=1.480,95% CI:1.088-2.012), and Internet access (OR=1.184,95% CI:1.015-1.381) are more willing to go to the grass-roots level for first diagnosis.

The first visit rate of elderly people at the grassroots level still needs to be improved. It is necessary to strengthen the publicity effect of grassroots first visit, improve the utilization rate of grassroots health services, encourage elderly people to use health intelligent devices, increase the construction of grassroots medical and health institutions in western regions, and correctly guide elderly people to form good medical habits.

To understand the status and problems of rehabilitation resource allocation in the system of the China Disabled Persons Federation and provide a reference for the rational allocation of rehabilitation resources in the system of the China Disabled Persons Federation.

Based on the health resource density index and rank sum ratio method, the rehabilitation resource allocation status of the disabled person's federation system in 31 provinces (municipalities) in my country in 2022 was comprehensively measured, analyzed, and evaluated.

The regression equation of Probit and RSR was RSR= -0.779+0.255*Probit value (F=1 206.958, P≤0.001), indicating that the regression line equation was statistically significant and representative. According to the optimal grading principle of the rank sum ratio method, the rehabilitation resource allocation status of the disabled person's federation system in my country was divided into three grades, with Probit>6 in 5 provinces and cities such as Shanghai, and a good allocation status; 4<Probit<6 in 22 provinces and cities (autonomous regions) such as Heilongjiang, and a medium allocation status; Probit<4 in 4 provinces and cities (autonomous regions) such as Tibet, and a poor allocation status.

The overall situation of rehabilitation resource allocation in the system of the disabled person's federation is good, but there is still a lack of total amount and differences between the East and West. Optimizing the inter-regional flow mechanism of rehabilitation service resources, improving the diversified pattern of rehabilitation resources in some provinces (cities), and promoting the efficient development of rehabilitation resource allocation are recommended.

To investigate the effect of sleep quality on suicide risk among medical students and the role of depression and academic stress in the relationship.

A questionnaire survey of 3 521 current medical students was conducted using the Pittsburgh Sleepiness Scale, the Patient Health Questionnaire Depression Scale, the Beck Suicidal Ideation Scale, and the Academic Stress Score. SPSS 26.0 was applied for descriptive statistics, independent samples t-test, ANOVA and correlation analysis of the data, and PROCESS plug-in was used for the moderated mediation test.

Sleep quality index positively predicted suicide risk（β=0.315）; depression partially mediated the association between sleep quality and suicide risk (β=0.233, 95% CI: 0.174-0.297), with a mediating effect accounting for 74.19% of the total effect; academic stress moderated the first half path of the mediation model (β=0.093), i.e., moderated the effect of sleep quality on depression.

Sleep quality increases the risk of suicide among medical students by affecting depression; reducing academic stress reduces the effect of sleep quality on depression among medical students.