To investigate the effect of Mulberry twig (Sangzhi) alkaloids (SZ-A), which is a new traditional Chinese medicinal, on myocardial fibrosis in diabetes mellitus (DM) and its underlying molecular mechanism.

The diabetic mouse model was established using high-fat diet combined with streptozotocin injections. Mice were randomly divided into non-diabetic (ND), diabetic (DM), and SZ-A low- (50 mg/kg/d) or high-dose (100 mg/kg/d) groups. Myocardial fibrosis-related proteins (Collagen Ⅰ, TGF-β1, Smad2/3, and their phosphorylated forms) were analyzed by Western blot, while mRNA levels of fibrosis markers (Collagen Ⅰ/Ⅲ, α-SMA, Fn1, Postn) were quantified via qRT-PCR. Molecular docking simulations (AlphaFold2/Autodock) were performed to validate interactions between SZ-A components (DNJ, FGM, DAB) and TGF-β1.

SZ-A treatment significantly reduced myocardial Collagen deposition in diabetic mice, downregulated mRNA expression of Collagen Ⅰ/Ⅲ, α-SMA, Fn1, and Postn (P < 0.05), and suppressed TGF-β1 expression and Smad2/3 phosphorylation. Molecular docking revealed stable binding affinities between DNJ/FGM/DAB and TGF-β1. Exogenous TGF-β1 administration reversed SZ-A's anti-fibrotic effects, restoring Collagen Ⅰ, TGF-β1, and p-Smad2/3 levels (P <0.05).

SZ-A ameliorates diabetic myocardial fibrosis by targeting the TGF-β1/Smad signaling pathway, highlighting its therapeutic potential for diabetic cardiomyopathy.

To explore the intervention mechanism of icaritin (ICT) on myocardial injury in sepsis.

Mice were divided into control group, model group, AMPK activator (MET) group, icaritin (ICT) group and icaritin+AMPK inhibitor compound C (ICT+CC) group, with 12 mice in each group. Except for the control group, the other mice were intraperitoneally injected with LPS. ICT group was intraperitoneally injected with ICT 30 minutes before modeling, and ICT+CC group was injected with AMPK inhibitor Compound C at the same time. After 24 hours of modeling, cardiac function, myocardial pathological changes, AMPK pathway and pyroptosis-related protein expression, serum inflammatory factors and myocardial injury markers were detected.

The intervention of ICT and AMPK activators significantly improved the survival rate of mice, increased cardiac LVEF and LVFS, and decreased LVIDD and LVIDS. Tissue damage was reduced and inflammatory cell infiltration was reduced. The levels of serum TNF-α, IL-1β, IL-18, CK-MB and LDH were decreased. The ratio of p-AMPK/AMPK was increased, and the expression of NLRP3, ASC, Cleaved Caspase-1, GSDMD-NT and Cleaved IL-1β was decreased (P < 0.05). Compound C could reverse the improvement effect of ICT on myocardial injury, increase inflammation index and pyroptosis protein expression (P <0.05).

ICT can improve myocardial injury and inflammatory response in sepsis mice, and its mechanism may be related to the activation of AMPK to inhibit pyroptosis mediated by NLRP3 signaling pathway.

To explore the clinical efficacy of Sancai acupuncture combined with Frontal-three-needles in treating insomnia of Heart-kidney disharmony Type.

According to the random number table method, 60 patients with heart kidney non overlapping insomnia treated at Bengbu TCM Hospital from January 2023 to February 2024 were divided into two groups. The control group of 30 cases received conventional acupuncture treatment, while the treatment group of 30 cases received a combination of Sancai acupuncture and frontal three acupuncture treatment. Both groups were treated for 4 weeks. The sleep quality, TCM syndrome scores, neurotransmitter levels, and clinical efficacy were analyzed.

After treatment, the scores of each dimension of PSQI and the total PSQI score in both groups decreased (P < 0.05), and the scores in treatment group were lower (P < 0.05). After treatment, the scores of traditional Chinese medicine syndrome in both groups decreased (P < 0.05), and the scores in treatment group were lower(P < 0.05). After treatment, the levels of serum5-HT, DA, and MT increased in both groups (P <0.05), and the indexes in treatment group were higher (P < 0.05). The effective rate in treatment group (93.33%) was higher compared with control group (73.33%) (P <0.05).

Sancai acupuncture combined with frontal-three-needles has a good therapeutic effect on insomnia of Heart-kidney disharmony type, which can improve sleep quality and neurotransmitter levels, and effectively reduce TCM syndrome scores.

To optimize the extraction process of total flavonoids from Mayaqi and study its antioxidant activity and stability.

Key factors were screened by Plackett-Burman experiment, and the optimal process was obtained by Box-Behnken Design surface design; The antioxidant activity of total flavonoids of Mayaqi was evaluated by measuring the scavenging effects on hydroxyl radicals, DPPH radicals, ABTS radicals and the ability to reduce ferric ions. The correlation between the concentration of total flavonoids extract and different antioxidant activities was by SPSS software, and the effects of temperature, light and pH on the stability of antioxidant activity were investigated.

The optimal extraction technology for total flavonoids from Mayaqi was as follows: extraction 140 W, ethanol concentration 65%, temperature 70 ℃, extraction time 30 min, and the extraction rate of total flavoids was 4.608 5 ±0.031 7 mg/g. The scavenging effects of the total flavonoids extract on Mayaqi DPPH radicals, and ABTS radicals, and the ability to reduce ferric ions were 64.16%、55.67%、95.97% and 50.49%, of the same concentration of Vc, respectively. Correlation showed that the concentration of the total flavonoids extract from Mayaqi was significantly correlated with the scavenging rate of xyl radicals. The antioxidant stability test showed that the total flavonoids extract from Mayaqi should be stored at low (below 25 ℃), away from light, and in neutral conditions.

This technology is stable and feasible, and the total flavonoids extract Mayaqi has high antioxidant activity.

To explore the clinical effect of combined therapy with Chifeng Yingyuan acupuncture method and traditional Chinese medicine Reyanbao therapy in the treatment of chronic lumbar muscle strain.

A total of 130 patients with chronic lumbar muscle strain in the hospital from From January 2022 to April 2025 were randomly grouped. Both groups received basic Western medicine treatment. On this basis, 65 cases in the control group were treated with the Chifeng Yingyuan acupuncture method, and 65 cases in the combined group were treated with the Chifeng Yingyuan acupuncture method combined with traditional Chinese medicine hot compress. The clinical efficacy, TCM syndrome scores, pain degree and lumbar function of the two groups were compared to evaluate the safety of the two groups.

Compared with the control group, the clinical efficacy in the combined group was enhanced after treatment while the TCM syndromes scores were reduced (P < 0.05). Compared with the control group, the Visual Analogue Scale (VAS) of the one-course and two-course combined groups after treatment decreased (P < 0.05); Compared with the control group, the Oswestry Disability Index (ODI) scores of the one-course and two-course combined groups after treatment decreased (P < 0.05); The adverse reactions revealed no significant differences between both groups (P > 0.05).

Chifeng Yingyuan acupuncture method combined with traditional Chinese medicine Reyanbao therapy has significant efficacy in the treatment of chronic lumbar muscle strain. Additionally, it can effectively relieve the pain, and promote the recovery of lumbar function, with high safety.

To observe the effects of self-formulated modified Tiaoying Decoction with modifications combined with herb-partitioned moxibustion on immune regulation, platelet parameters and the number and function of endothelial progenitor cells in peripheral blood of patients with myeloid suppression anemia after chemotherapy for advanced gastric cancer.

We selected 104 patients with myelosuppressive anemia after chemotherapy for advanced gastric cancer who were treated in Hengshui Hospital of Traditional Chinese Medicine from April 2022 to April 2024. They were divided into a control group of 52 cases (3 cases dropped out, and finally 49 cases were included) and a treatment group of 52 cases (4 cases dropped out, and finally 48 cases were included) by the random number table method. Both groups were given routine Western medicine treatment simultaneously. The treatment group was given herb-partitioned moxibustion combined with self-formulated modified Tiaoying Decoction with modifications on the basis of routine Western medicine treatment. After the treatment course, the clinical efficacy of the two groups was compared. Before and after treatment, the scores of various traditional Chinese medicine syndromes were observed. The quality of life was evaluated by the Functional Assessment of Cancer Therapy-General (FACT-G). The levels of neutrophils, hemoglobin, white blood cell count and platelet count of patients were detected. The number and migration ability of circulating endothelial progenitor cells in peripheral blood were detected. The levels of reactive oxygen species (ROS), granulocyte-macrophage colony-stimulating factor (GM-CSF), Notch2, Numb1, CD4⁺ cells, CD8⁺cells and CD16⁺ CD56⁺ cells in patients were detected. The 1-year recurrence rate and 1-year survival rate of the two groups of patients were compared.

The treatment group's total effective rate was 95.83%. The control group's total effective rate was 79.59%. The treatment group's total effective rate was higher than the control group (P < 0.05). The treatment group's scores of TCM syndrome was lower than control group (P < 0.05). The treatment group's FACT-G scores was higher than the control group (P <0.05). The treatment group's neutrophil, hemoglobin, white blood cell count and platelet count in treatment group levels were higher than control group (P < 0.05). The treatment group's number and migration ability of peripheral blood circulating endothelial progenitor cells were lower than control group (P <0.05). The treatment group's CD4⁺ cells and CD16⁺ CD56⁺ cells levels were higher than control group (P < 0.05). The treatment group's expression of CD8⁺ cells was lower than control group (P < 0.05). The treatment group's ROS, GM-CSF and Notch2 expression levels were lower than control group (P < 0.05). The treatment group's expression level of Numb1 was higher than that in the control group (P < 0.05). The 1-year recurrence rate of the treatment group was 31.25%, which was lower than 53.06% of the control group (P < 0.05), and the 1-year survival rate of the treatment group was 97.92%, which was higher than 81.63% of the control group (P < 0.05).

Self-formulated modified Tiaoying Decoction with modifications combined with herb-partitioned moxibustion can reduce the oxidative stress response, regulate the immune function and platelet parameters of patients, improve the number and function of peripheral blood endothelial progenitor cells, alleviate the disease, and improve the clinical efficacy and quality of life in the treatment of patients with advanced gastric cancer after chemotherapy.

To explore the efficacy of Fuzheng Qufeng Decoction in patients with chronic urticaria, its effects on the Weekly Urticaria Activity Score (UAS7), Dermatology Life Quality Index (DLQI), and serum C-reactive protein (CRP) and immunoglobulin levels. Additionally, it evaluated the decoction's regulatory effects on serum inflammatory factors, chemokines, and immune function.

This prospective study enrolled 211 patients with chronic urticaria treated at our hospital from March 2020 to March 2024. Patients were randomly assigned into a control group (n=106), receiving levocetirizine hydrochloride tablets, and an observation group (n=105), receiving Fuzheng Qufeng Decoction in combination with levocetirizine hydrochloride. Both groups were treated for 4 weeks. UAS7 and DLQI scores were evaluated before and after treatment in both groups. Traditional Chinese Medicine (TCM) symptom scores, serum levels of inflammatory cytokines, chemokines, and immune function-related indicators were measured and compared between the two groups. The efficacy and incidence of adverse reactions were statistically analyzed.

After treatment, both the UAS7 and DLQI scores decreased in the observation group and the control group, with median UAS7 and DLQI scores in the observation group being 7.00 and 2.00, respectively, both lower than the control group's 7.50 and 3.00 (P < 0.05). The primary and secondary symptom scores of Traditional Chinese Medicine (TCM) syndromes also decreased in both groups, with the observation group showing significantly lower scores than the control group (P <0.05). Post-treatment, serum levels of IL-17, IL-23, IL-31, IL-33, IFN-γ, TGF-β, CRP, SAA, D-dimer, MCP-1, RANTES, Eotaxin, C5a, and IgE were all lower in the observation group compared to the control group (P <0.05), while serum C3 and C4 levels were higher in the observation group (P <0.05). The total treatment efficacy rate in the observation group was higher than that in the control group. The total incidence of adverse reactions in the observation group was lower than that in the control group (P < 0.05).

Fuzheng Qufeng Decoction significantly improved clinical symptoms, reduced UAS7 and DLQI scores, lowered serum levels of inflammatory cytokines and chemokines, enhanced immune function, and demonstrated high safety with fewer adverse reactions. It holds substantial clinical value for treating chronic urticaria.