To establish the processing method of the classic wine angelica in Wenjing decoction and the HPLC fingerprint of the decoction pieces of angelica in order to provide reference for the follow-up Wenjing decoction preparation research.

By consulting ancient books and combining modern technology and methods, the washing, moisturizing, cutting, drying and other processes were optimized to obtain angelica tablets. The dosage and moistening time of Chinese angelica yellow wine were optimized through single factor investigation. Combining "gentel fire dry" operation key points, three processing parameters, i.e., frying temperature, frying time and frying speed, were observed by L₉(3⁴) orthogonal experiment. Taking traits, moisture (%), extract (%) and ferulic acid content (%) as the inspection indicators, the entropy weight method is used to calculate the composite score, and the processing technology of distilled angelica is optimized. The fingerprint of Danggui decoction pieces was established by HPLC using Techmate C₁₈ column (250 mm×4.6 mm, 5 μm), and acetonitrile (A)-0.2% phosphoric acid water (B) was used as the mobile phase system for gradient elution. The detection wavelength was set at 280 nm, the column temperature was 30 ℃, the flow rate was 1.0 mL·min^-1.

The best processing method of Angelicae sinensis Radix processed with yellow wine and the HPLC fingerprint of decoction pieces of Angelicae sinensis Radix processed with yellow wine were established, and 4 main common peaks were identified, namely chlorogenic acid, ferulic acid, ligustilide, and ligustalide I. The content determination methods of the four components were established.

The established processing method of Angelicae sinensis Radix using yellow wine is stable and feasible, and its fingerprint can provide a reference for the development and research of Wenjing decoction.

Insulin degludec and insulin detemir are the third generation of insulin analogues that are chemically modified on long chain aliphatic acid. It is necessary to establish a specific and sensitive liquid chromatography-tandem mass spectrometry method for the determination of amino acid sequences of chemically modified insulin analogues.

The separation was achieved by an ACQUITY UPLC peptide BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm,300 Å) with a mobile phase of 0.1% FA/H₂O as Solvent A and 0.1% FA/acetonitrile as Solvent B in a gradient mode. The flow rate was set at 0.3 mL·min^-1. MS^E was used as the detection mode of mass spectrometry, and the collision energy was 30~40 eV.

The coverage rate of insulin degludec and insulin detemir sequences was good as expected, and the fragment ion y1& was detected by chemical modification. RSD% of ionic strength of medium and high strength ions was within 15%. Both insulin sequences can be effectively distinguished it from other unmodified ones.

A liquid mass spectrometry method for amino acid sequences of adipose-side chain modified insulin analogs with good repeatability, specificity and durability was established, providing reference for the research method of sequence structure of this kind of products.

To prepare and optimize the formulation of resveratrol in situ floating gel using common gel materials with high safety and good biocompatibility, in order to obtain a temperature-sensitive gel that can float and continuously release drug in bladder and can be used for bladder perfusion in chemotherapy of bladder cancer.

The gel was prepared by cold dissolution method. Poloxam 407, Poloxam 188, sodium alginate, calcium carbonate and ammonium bicarbonate were used as main excipients. Gelling temperature and gelling time were selected as the indicators to optimize the quantity of poloxamer 407, poloxamer 188 and calcium carbonate in formulation by central combination-response surface method. Temperature sensitivity, floating performance and drug releasing performance of the optimized formulation were evaluated.

The optimal formulation was composed of resveratrol 30%, Poloxam 407 17.2%, Poloxam 188 5.5%, sodium alginate 0.15%, calcium carbonate 0.06%, ammonium bicarbonate 0.15%. The gelling temperature was 28~29 ℃ and the gelling time was 50 s. The drug were released from the gel in a way of diffusion for more than 48 days.

The resveratrol in situ floating gel had the property of continuous drug release for a long time in bladder. It can be used as an alternative form of bladder perfusion chemotherapy in the treatment of bladder cancer to improve patient compliance.

To analyze the characteristics of perampanel-related treatment emergent adverse events (TEAEs), providing references for clinical rational drug use.

The literatures about TEAEs caused by perampanel were retrieved from databases like CNKI, Wanfang, VIP, PubMed, ScienceDirect and Web of Science. The cases were divided into treatment group and overdose group according to the drug dose.

A total of 20 literatures including 25 cases were reported, with 14 cases in the treatment group and 11 cases in the overdose group. Most TEAEs occurred within 60 days after drug administration in the treatment group (8 cases, 32.00%), mainly involving TEAEs in mental system (7 cases, 28.00%) and nervous system (3 cases, 12.00%). The TEAEs occurred in the treatment group were relieved after withdrawal and/or symptomatic treatment. All TEAEs mainly occurred in nervous system (9 cases, 36.00%) and the mental system (5 cases, 20.00%) on the day of medication in the overdose group, among which 9 cases were completely improved, and 2 cases had sequelae after treatment.

TEAEs caused by perampanel should be paid attention to, and medication education should be strengthened to avoid the occurrence of serious TEAEs and ensure the safety of clinical medication.

to investigate the therapeutic effect and possible mechanism of baicalein on ulcerative colitis induced by dextran sulfate sodium salt (DSS) in mice.

A total of 42 male BALB/c mice were randomly divided into 6 groups (n=7), including control group, model group, baicalein groups (low-dose, medium-dose and high-dose) and mesalazine group (positive). The UC model of mice was established by 4% DSS. After the model was successfully established, the control group and the model group were given distilled water ad libitum, baicalein suspension of 10, 20, and 40 mg·kg^-1, and the positive drug mesalazine 600 mg·kg^-1, ig, for 14 days. The disease activity index (DAI) was calculated and evaluated, the changes of colon histopathology were observed by hematoxylin-eosin (HE) staining, and the levels of lipopolysaccharide (LPS) and secretory immunoglobulin A (sIg A) in serum were measured by ELISA. The expression levels of interleukin-6 (IL-6), tumor necrosis factor α (TNF-α) and nuclear factor of activated B(NF-κB) were detected by Western blotting.

Baicalein decreased DAI index of UC mice, decreased the protein expressions of IL-6, TNF-α and NF-κB in colon tissue, decreased the serum levels of LPS, and increased the content of sIg A in serum.

Baicalein reduced the inflammatory response and enhanced the immunity of UC mice, and the mechanism is related to regulating the expression of NF-κB in colon.

To investigate whether licorice extracts (LE) combined with iron death inducer Erastin can induce iron death of colorectal cancer cell DLD1 through dipeptidyl peptidase-4 (DPP4).

DLD1 cells were randomly divided into control group, L-LE group (50 μg·mL^-1 LE), H-LE group (100 μg·mL^-1 LE), H-LE+Erastin group (100 μg·mL^-1 LE+30 nmol·L^-1 Erastin) and H-LE+Erastin+Sita (DPP4 inhibitor) group (100 μg·mL^-1 LE+30 nmol·L^-1 Erastin+31.25 μg·mL^-1 Sita), with drug interventions for 24 h. Cell cloning assay and Edu-594 cell proliferation test were conducted to determine the cloning and proliferation abilities of the cells. ELISA assay was used to detect the Fe²⁺ content and DPP4 activity. Flow cytometry was used to detect reactive oxygen species(ROS) content. Western blotting was used to detect the expression of solute carrier family 7 member 11(SLC7A11) and arachidonate lipoxygenase 3(ALOXE3) proteins.

Compared with control group, the cell cloning number decreased while the ROS content increased in L-LE group, H-LE group and H-LE+Erastin group (P<0.05 or P<0.01); and the proliferation ability and Fe²⁺ content decreased, DPP4 activity and ALOXE3 protein expression increased, SLC7A11 protein expression decreased in H-LE and H-LE+Erastin groups (P<0.05 or P<0.01). Compared with H-LE+Erastin group, the addition of Sita significantly increased cell cloning, proliferation and SLC7A11 protein expression, while significantly decreased Fe²⁺ content, DPP4 activity, ROS content and ALOXE3 protein expression (P<0.01).

LE combined with Erastin can induce iron death of colorectal cancer (CRC) cell through DPP4.