This study aims to systematically compare the differences in cytotoxicity evaluation of disposable infusion sets between the weight-based and inner surface area-based extraction methods, as well as between the two standards GB/T 16886.5—2017 and GB/T 14233.2—2005. The goal is to establish an extraction protocol that more accurately reflects the clinical biological safety risk characteristics.

A clinically standard disposable infusion set was selected as the research object. Based on the two standards, two extraction solutions were prepared. ①Weight-based method: 0.2 g/mL of the cut material added to MEM medium containing serum; ②Inner surface area method: 6 cm²/mL of MEM medium containing serum filled into the lumen of the infusion set. Both were extracted under shaking conditions (37 ℃, 60 rpm) for 72 hours. The extracts were then subjected to comparative in vitro cytotoxicity testing.

Qualitative results indicated that cytotoxic responses varied under different standards. According to GB/T 16886.5—2017, the 100% extracts from both extraction methods exhibited mild cytotoxicity (Grade 2), whereas under GB/T 14233.2—2005, they both showed moderate cytotoxicity (Grade 3). Quantitative results revealed that, regardless of the standard used, the inner surface area extraction method consistently yielded higher cytotoxicity than the weight-based method. Notably, under GB/T 16886.5—2017, a significant difference in cell viability was observed between the two extraction methods (P<0.001).

For lumen-type devices such as infusion sets, employing the inner surface area extraction method in strict accordance with GB/T 16886.5 provides a more realistic reflection of clinical biological safety risks. It is also crucial to recognize methodological differences between standards. The more sensitive GB/T 14233.2 is recommended for risk screening during product release and batch consistency monitoring. Conversely, for medical device registration review and systemic biological evaluation, a comprehensive assessment strategy based on the inner surface area extraction within the GB/T 16886.5 framework should be prioritized.

A method for the simultaneous determination of 5 fat-soluble vitamins [VA, 25-(OH)D₂, 25-(OH)D₃, VE, VK] in serum was established based on the liquid chromatography-tandem mass spectrometry, and a systematic methodological validation was conducted.

After the samples were subjected to protein precipitation and centrifugation, the supernatant was collected. A Phenomenex Kinetex column C18 (2.6 μm F5 100A, 3.0 mm×50 mm) was used, with the column temperature set at 40 ℃. Mobile phase A was 0.1% formic acid aqueous solution, and mobile phase B was 0.1% formic acid methanol solution, with a flow rate of 0.7 mL/min and an injection volume of 25 μL. Positive ion mode with multiple reaction monitoring (MRM) was adopted, and quantification was performed using the internal standard method. Indicators such as the precision, spiked recovery rate, and anti-interference ability of the method were verified.

The 5 fat-soluble vitamins showed good linear relationships within their respective concentration ranges, with correlation coefficients all greater than 0.998. The intra-day precisions were in the range of 1.71%~6.25%, and inter-day precisions were in the range of 2.71%~6.76%. The spiked recovery rates were in the range of 89.65%~110.61%. The interference biases caused by substances such as hemoglobin, triglycerides, and bilirubin were all less than 10%.

This method is rapid in analysis, high in sensitivity, and strong in anti-interference ability. It can quickly and accurately determine the levels of 5 fat-soluble vitamins in serum, and is suitable for routine detection of clinical batch samples.

To compare the dosimetric differences between dynamic intensity-modulated radiotherapy (dIMRT) and static intensity-modulated radiation therapy (sIMRT) with varying minimum segment area (MSA) parameters, using 6 MV flattening filter-free (FFF) photon beams for left-sided early-stage breast cancer patients after breast-conserving surgery.

A retrospective study was conducted using clinical imaging and treatment data from twelve female patients with left-sided early-stage breast cancer, who were treated at the Third People's Hospital of Bengbu from March 2024 to March 2025. Each patient received dIMRT and sIMRT (MSA values of 0.3, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0, and 10.0 cm² were applied) plans in FFF mode using treatment planning system of United Imaging. The prescription dose was 50 Gy, delivered at 2 Gy per time, 5 times per week. Plan comparisons included the dose distribution in the target volume, dose to organs at risk (OARs), skin dose (skin 0~2, skin 2~5, skin 5~8), plan efficiency [monitor units (MUs), delivery time], and gamma passing rates (1 mm/1% criteria).

dIMRT demonstrated slightly superior target coverage (V₉₅, D_mean, HI) and significantly improved V₁₀₇ and D_max compared to sIMRT (P<0.05). The D_mean of heart was significantly lower in dIMRT (P<0.05). The D_mean of skin doses were reduced in dIMRT (P>0.05). Although dIMRT required higher MUs, it reduced delivery time by 25% (P<0.05). γ passing rates under 1 mm/1% criteria were marginally lower for dIMRT (MSA≤5.0 cm², P<0.05).

For early-stage left-sided breast cancer patients receiving IMRT after breast-conserving surgery under the FFF mode on the United Imaging platform, dIMRT demonstrates superiority over sIMRT in terms of target dose distribution, dose sparing of OARs, and delivery efficiency. It is therefore recommended that dIMRT be preferentially considered for treatment planning under similar equipment and clinical conditions.

To investigate the application effects of an intervention combining nostalgia therapy and intelligent rehabilitation gloves training on alleviating negative emotions in older stroke patients, and to evaluate its feasibility and promotion value in primary care institutions.

A total of 60 older stroke patients were recruited and randomly assigned to either an experimental group or a control group, with 30 cases in each group. Participants in the control group received training using a pneumatic glove under two modes: “bilateral mirror training” and “active game playing”, each for 15 minutes per session, once daily, five days per week, for a total of three weeks. The experimental group received additional nostalgia therapy training for 30 minutes per session, once daily, five days per week, also over three weeks. All participants were assessed before and after the intervention using the following scales: anxiety was evaluated with the generalized anxiety disorder-7 (GAD-7) and the self-rating anxiety scale (SAS); depressive symptoms were measured using the geriatric depression scale (GDS), the Montgomery–Asberg depression rating scale (MADRS), the patient health questionnaire-9 items (PHQ-9), and the Chinese version of the 10-item stroke aphasic depression questionnaire–hospital version (SADQ-H10); activities of daily living were assessed with the modified Barthel index (MBI).

After three weeks of intervention, both groups demonstrated significant improvements in GAD-7, SAS, GDS, MADRS, PHQ-9, SADQ-H10, and MBI scores compared with baseline (P<0.05). The experimental group showed significantly greater improvements than the control group across all scales (P<0.05). Comparison of post-intervention progress revealed that the experimental group achieved superior improvements in SAS, GDS, MADRS, SADQ-H10, and MBI scores relative to the control group (P<0.05).

For older stroke patients presenting with negative emotions such as anxiety and depression, the intervention combining nostalgia therapy and intelligent rehabilitation gloves training can effectively alleviate their anxiety and depressive states, enhance their motivation for rehabilitation, and thereby further accelerate the recovery process. This intervention demonstrates good potential for promotion in primary healthcare institutions, and future research should further explore its application in community-based rehabilitation settings.

To explore the application value of multiparametric MRI techniques, including conventional MRI, contrast-enhanced MRI (CE-MRI), and dynamic contrast-enhanced MRI (DCE-MRI), in differentiating benign from malignant thyroid nodules.

A retrospective analysis was performed on 74 patients with 89 thyroid lesions who were confirmed by surgical pathology with definite pathological results and preoperative magnetic resonance imaging. Observe the nodule boundary and whether the nodule has become cystic. Nodule size, apparent diffusion coefficient (ADC), and various quantitative parameters including volume transfer constant (K^trans), extracellular space volume fraction outside blood vessels (V_e), vascular return constant (K_ep), initial area under the gadolinium-concentration–time curve (iAUC) from DCE-MRI were measured, and recorded. Independent sample t-tests and Mann-Whitney U tests were used to compare parameters between benign and malignant thyroid nodules. Multifactorial logistic regression was employed to select independent predictive factors and establish a combined diagnostic model, with ROC curves analyzed to assess the performance of individual parameters and the combined model.

Differences in lesion margin, ADC, K^trans, and iAUC between benign and malignant thyroid nodules were all statistically significant (P<0.05). Multivariate logistic regression with stepwise selection identified iAUC, ADC, and margin status as the optimal predictor set; the corresponding odds ratios were 0.890, 0.984, and 64.759, respectively. The area under the ROC curve for this model was 0.986, with a sensitivity of 94.1%, and a specificity of 89.4%. Internal validation demonstrated excellent generalizability, with an AUC of 0.979 in the validation cohort.

This study shows that multiparametric MRI can improve the ability to distinguish between benign and malignant thyroid nodules. A combined diagnostic model incorporating independent risk factors such as iAUC value, ADC value, and boundary clarity has a high diagnostic value.

To investigate the value of ultrafast pulse wave velocity (ufPWV) combined with hemodynamic parameters in the prognostic assessment of patients with acute ischemic stroke (AIS).

A total of 65 patients with AIS admitted to our institution from August 2023 to August 2025 were enrolled as study subjects. The ufPWV technique was employed to assess the carotid artery pulse wave velocity during the beginning of systole (BS) and end of systole (ES). These measurements were combined with evaluations of intima-media thickness (IMT) and hemodynamic parameters, including peak systolic velocity (PSV) and end diastolic velocity (EDV). Clinical baseline data were also collected. Based on prognosis, the patients were categorized into a favorable prognosis group and a poor prognosis group. The value of each indicator in the diagnosis and prognostic prediction of AIS was analyzed.

The IMT, BS, ES, PSV and EDV were significantly higher in the unfavorable prognosis group compared to the favorable prognosis group (P<0.05). Age, fasting blood glucose and National Institute of Health stroke scale (NIHSS) score at admission were also significantly higher in the unfavorable prognosis group (P<0.05). Multivariate logistic regression analysis revealed that elevated levels of fasting blood glucose, BS, ES, PSV, and NIHSS score were risk factors for poor prognosis. Receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive value of BS, ES and PSV for an unfavorable prognosis in AIS patients, with AUC values of 0.767, 0.810 and 0.641, respectively. The combined detection of these three parameters yielded an AUC of 0.885 for predicting an unfavorable prognosis.

The ufPWV parameters BS and ES, along with the hemodynamic parameter PSV are risk factors for poor prognosis in AIS and hold certain value in prognostic assessment of AIS.

To compare the efficacy and safety of musculoskeletal ultrasound-guided and blind injection in the treatment of subacromial and subdeltoid bursitis.

The clinical data of 136 patients with subacromial and subdeltoid bursitis admitted to our hospital from January 2023 to August 2025 were retrospectively selected. According to different treatment methods, they were divided into the study group (n=75) and the control group (n=61). Patients in the control group were treated with local block injection, while patients in the study group were treated with subacromial-deltoid subcapsular injection therapy guided by musculoskeletal ultrasound. The clinical efficacy, the incidence of adverse reactions and the rate of secondary injection of the two groups of patients were compared after 2 weeks of treatment. The visual analogue scale (VAS) score was used to evaluate the degree of pain before treatment, 1 day after treatment, 1 week after treatment and 4 weeks after treatment. Before treatment and 2 weeks after treatment, upper limb function was evaluated using the upper limb simplified Fugl-Meyer assessment scale, and shoulder joint function was evaluated using the UCLA shoulder rating scale (UCLA).

The total clinical effective rate of the study group was significantly higher than that of the control group (P<0.05). One day after treatment and one week after treatment, the VAS score of the study group was lower than that of the control group (P<0.05). After 2 weeks of treatment, the Fugl-Meyer score and UCLA score of the upper limbs in the study group were higher than those in the control group (P<0.05). The rate of the incidence of adverse reactions and secondary injection in the study group were both lower than those in the control group (P<0.05).

The clinical efficacy of minimally invasive treatment of subacromial and subdeltoid bursitis patients under musculoskeletal ultrasound guidance is superior to that of traditional blind injection. It can relieve pain more effectively, improve shoulder joint function and upper limb function, and has a low need for secondary treatment and high safety.

To explore the effects of ultrasound-guided transversus abdominis plane block combined with general anesthesia on patients undergoing laparoscopic cholecystectomy (LC).

From January 2022 to January 2025, 124 patients who underwent LC treatment in our hospital were randomly divided into two groups, with 62 cases in each group. The control group received simple general anesthesia, while the observation group received ultrasound-guided transversus abdominis plane block combined with general anesthesia. The hemodynamic indicators, analgesic effect, postoperative recovery indicators, and adverse reactions were compared between the two groups.

Immediately after tracheal intubation (T1), the establishment of pneumoperitoneum (T2), and at the end of the operation (T3), the mean arterial pressure (MAP) and heart rate (HR) of the observation group were higher than those of the control group (P<0.05); at 1 h, 6 h, 12 h, and 24 h after surgery, the visual analogue scale (VAS) scores of the observation group were lower than those of the control group (P<0.05); the postoperative spontaneous breathing recovery time, extubation time, and awakening time of the observation group were shorter than those of the control groupt (P<0.05); the incidence of adverse reactions in the observation group was lower than that in the control group (P<0.05).

Ultrasound-guided transversus abdominis plane block combined with general anesthesia for LC patients can stabilize hemodynamics, improve analgesic effect, and have a fast postoperative recovery and higher safety.

To evaluate the value of low-dose CT (LDCT) scan combined with artificial intelligence (AI) analysis in the screening and follow-up of pulmonary nodules.

A total of 160 patients with pulmonary nodules who underwent LDCT screening and completed at least one follow-up between January 2023 and June 2025 were selected. All of them underwent LDCT examination and the lung nodule artificial intelligence imaging-assisted diagnosis system was used. The pathological results were used as the gold standard to compare the detection rates of different diameter nodules, the tracking success rates of the same nodules, and the time spent on reading films between AI reading and manual reading.

AI reading showed higher detection rates for nodule diameter <3 mm and [3, 5) mm than manual reading (P<0.05). During follow-up, AI achieved higher tracking success rates of the same nodule (P<0.05). AI also required markedly less reading time (P<0.001).

The LDCT scan combined with AI analysis has significant application value in the diagnosis of pulmonary nodules, especially in the identification of changes in small nodules before and after re-examination, it demonstrates high sensitivity and efficiency. It has unique advantages in saving time and standardizing the diagnostic process, and is worthy of clinical promotion.

To explore the clinical efficacy of optimal pulse technology (OPT) combined with 0.1% fluorometholone eye drops in the treatment of dry eye related to Sjögren's syndrome.

One hundred patients with Sjögren's syndrome-related dry eye treated in our hospital from March 2021 to March 2025 were selected as the study subjects and randomly divided into a control group and an experimental group, with 50 patients in each group. The control group received 0.3% sodium hyaluronate eye drops, 0.1% fluorometholone eye drops combined with standardized meibomian gland massage and warm compress. The experimental group received the same treatment as the control group, with the addition of OPT treatment. The tear film break-up time (BUT), tear meniscus height (TMH), results of the Schirmer I test (SIt), ocular surface disease index (OSDI) score, corneal fluorescein staining (FL) score, emotional status [self-rating anxiety scale (SAS) score and self-rating depression scale (SDS) score], and treatment efficacy were compared between the two groups.

After treatment, the experimental group exhibited significantly higher values in BUT, TMH, and SIt compared to the control group, with statistically significant differences (P<0.05). The OSDI and FL scores of the experimental group were significantly lower than those of the control group after treatment, showing statistically significant differences (P<0.05). Furthermore, the SAS and SDS scores of the experimental group were significantly lower than those of the control group after treatment, with statistically significant differences (P<0.05). The clinical efficacy of the experimental group was significantly higher than that of the control group, and the difference was statistically significant (P<0.05).

The combination of optimal pulse technology and fluorometholone eye drops in the treatment of dry eye related to Sjögren's syndrome can effectively improve the clinical symptoms of patients, reduce their negative emotions, and has a good therapeutic effect, which has clinical promotion value.