Full
Outline
A method for the simultaneous determination of 5 fat-soluble vitamins [VA, 25-(OH)D2, 25-(OH)D3, VE, VK] in serum was established based on the liquid chromatography-tandem mass spectrometry, and a systematic methodological validation was conducted.
After the samples were subjected to protein precipitation and centrifugation, the supernatant was collected. A Phenomenex Kinetex column C18 (2.6 μm F5 100A, 3.0 mm×50 mm) was used, with the column temperature set at 40 ℃. Mobile phase A was 0.1% formic acid aqueous solution, and mobile phase B was 0.1% formic acid methanol solution, with a flow rate of 0.7 mL/min and an injection volume of 25 μL. Positive ion mode with multiple reaction monitoring (MRM) was adopted, and quantification was performed using the internal standard method. Indicators such as the precision, spiked recovery rate, and anti-interference ability of the method were verified.
The 5 fat-soluble vitamins showed good linear relationships within their respective concentration ranges, with correlation coefficients all greater than 0.998. The intra-day precisions were in the range of 1.71%~6.25%, and inter-day precisions were in the range of 2.71%~6.76%. The spiked recovery rates were in the range of 89.65%~110.61%. The interference biases caused by substances such as hemoglobin, triglycerides, and bilirubin were all less than 10%.
This method is rapid in analysis, high in sensitivity, and strong in anti-interference ability. It can quickly and accurately determine the levels of 5 fat-soluble vitamins in serum, and is suitable for routine detection of clinical batch samples.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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