To evaluate the diagnostic value of multiplex PCR for detecting common pathogens in children with respiratory infections.

We collected throat swab samples from 265 children with respiratory infections admitted to Tongshan Maternal and Child Health Hospital in Xuzhou between May 2023 and May 2024. These samples were tested using both multiplex PCR and direct immunofluorescence assay (DFA). Analyze the differences in detection results between multiplex PCR and DFA for respiratory pathogens.

For the 265 pediatric samples, multiplex PCR detected 98 positive cases and 167 negative cases, while DFA identified 81 positive cases and 184 negative cases. The number of positive cases detected by multiplex PCR was significantly higher than those detected by DFA, with a statistically significant difference (P<0.05). The detection results for RSV、FluA and FluB showed high consistency between the two methods. In contrast, the consistency of detection for ADV、PIV1 and PIV3 was ordinary. Multiplex PCR demonstrates superior sensitivity, specificity, and accuracy compared to DFA for the detection of RSV, ADV, FluA, FluB, HPIV1, HPIV2, and HPIV3. ROC analysis demonstrated that multiplex PCR exhibited superior diagnostic performance for respiratory pathogens compared to DFA (P<0.05). The AUC for multiplex PCR was 0.981, whereas for DFA, it was 0.799.

Multiplex PCR enables the simultaneous detection of multiple pathogens with high diagnostic efficiency, which is crucial for accurately diagnosing common respiratory infections in children.

To explore the difference between two dosimetric verification methods by using the IBA MatrixX in the verification of dynamic and static intensity modulated plan for breast tumor.

A total of 20 breast tumor patients treated with postoperative radiotherapy in our hospital form November 2023 to October 2024 were selected. The integrated dosimetric measurement of static intensity modulation (sIMRT) and dynamic intensity modulation (dIMRT) treatment plans for each patient were measured with the gantry fixed at 0 degree for all beams and actual gantry. According to different 1mm/1%, 2mm/2%, 2 mm/3% and 3mm/3% standards, the γ passing rate of each measurement result was analyzed.

Under the 3mm/3% standard, the γ passing rate of each measurement result was greater than 95%, which meets clinical requirements. The γ passing rate with the continuous improvement of the standard, the differences in dose distribution gradually become more prominent, and the γ passing rate gradually deteriorates. Under the same intensity modulation mode, the gantry Angle zeroing group was significantly better than the actual gantry group, and the difference was statistically significant (P<0.05). There was no significant difference between sIMRT and dIMRT under 2mm/2%, 2mm/3%, 3mm/3% standard conditions with the same verification method and different intensity modulation modes (P<0.05). At the 1mm/1% standard, the BCS_sIMRT_0 was better than the BCS_dIMRT_0, and the RM_sIMRT was better than RM_dIMRT, and the difference was statistically significant (P<0.05). In the BEV direction, the average maximum vertical distances from the PTV edge to the center of the shooting field in X and Y axis directions were (9.80±1.72) cm and (7.18±1.48) cm in BCS group, and (12.89±2.13) cm and (9.48±0.98) cm in RM group, respectively. Actual gantry Angle multi-field combination verification method γ pass rate is not affected by PTV edge to X wheelbase separation (P<0.05), but the γ pass rate of RM group was negatively correlated with PTV edge to Y wheelbase departure (r<0) and were statistically significant (P<0.05).

When validating the IMRT radiotherapy plan for breast tumor in the tangent field off the center, if selecting the 3mm/3% standard, the verification method of actual rack angle multi field synthesis that is more in line with the real irradiation situation can be used, or selecting the 2mm/2% and 2mm/3% standards, the verification method of 0-angle can be chosen.

To explore the application of improved E-word intraoral scanning in the production of orthodontic digital impression.

A total of 120 patients who underwent orthodontic treatment with digital impression technology in Nanjing Stomatological Hospital, Medical School of Nanjing University from March 2022 to March 2024 were selected. According to the random number table method, they were divided into the experimental group (the scanning method was modified E-word intraoral scanning) and the control group (the scanning method was segmented method), with 60 cases in each group. The scanning time, scanning comfort, orthodontic effect and satisfaction were compared between the two groups.

The scanning time of the experimental group was significantly shorter than that of the control group, and the comfort score was significantly higher than that of the control group, with statistically significant differences (P<0.05). The total effective rate of the experimental group was higher than that of the control group, with a statistically significant difference (P<0.05). The satisfaction rate of the experimental group was higher than that of the control group, with a statistically significant difference (P<0.05).

The modified E-word intraoral scanning can significantly improve the scanning efficiency, patient comfort, orthodontic effect, satisfaction, and improve the impact of oral health problems on the quality of life.

To explore the clinical efficacy of 3D thoracoscopic pulmonary nodule removal in the treatment of pulmonary nodules and to observe the complications of patients.

Seventy patients admitted to Zhaoqing Hospital of Traditional Chinese Medicine, Guangdong Province from March 2022 to February 2024 who underwent 3D thoracoscopic minimally invasive removal of pulmonary nodules were selected as the experimental group, and 70 patients who underwent traditional surgery during the same period were selected as the reference group. The surgical-related indicators, pulmonary function indicators, pain scores and complication occurrence of the two groups of patients were compared.

The operative time, intraoperative blood loss, postoperative drainage volume and hospital stay of experimental group were all lower than those of the reference group (P=0.000). After intervention, the levels of FVC, FEV₁ and PEF in experimental group were higher than those in reference group (P<0.05). The scores of test group at 2d, 5d and 7dVAS after surgery were significantly lower than those of the control group (P=0.000). The complication rate of experimental group was lower than that of control group (P=0.016).

3D thoracoscopic minimally invasive removal is effective in the treatment of pulmonary nodules, which can shorten the operation time and hospital stay, reduce intraoperative blood loss, improve lung function, relieve patients' pain and reduce complications, and is worthy of further promotion and application.

To investigate the clinical efficacy of medical infrared cough gel combined with recombinant human interferon α2b aerosol inhalation in the treatment of chronic bronchitis to alleviate patients' suffering and improve their quality of life.

This was a retrospective study of the clinical data of 120 patients with chronic bronchitis admitted to Shenzhen Renkang Hospital from January 2022 to January 2024. The patients were divided into the experimental group and the control group according to different treatment methods, with 60 patients in each group. The control group was treated with recombinant human interferon α2b aerosol inhalation, and the observation group was treated with medical infrared cough gel in addition to the control group, and both groups were treated for 5～7 days. The clinical efficacy, improvement time of clinical symptoms, hospitalization time, and pulmonary function indicators were observed and compared.

The total effective rate of the observation group after treatment was higher than that of the control group, with a statistically significant difference (P<0.05). The time for the disappearance of clinical symptoms and the hospitalization time in the observation group were both shorter than those in the control group, with statistically significant differences (P<0.05). After treatment, the levels of FEV₁/FVC, FEV₁%pred, MVV, tidal volume, peak time ratio, and peak volume ratio in the observation group were all higher than those in the control group, and the level of serum creatinine in the observation group was lower than that in the control group, with statistically significant differences (P<0.05). After treatment, the levels of interleukin-18, interleukin-17, and C-reactive protein in the observation group were lower than those in the control group, with statistically significant differences (P<0.05).

The combined use of medical infrared cough-relieving gel and recombinant human interferon α₂b aerosol inhalation therapy for chronic bronchitis has significant effects, can effectively improve clinical symptoms, shorten recovery time, and improve pulmonary function indicators.

To explore the clinical value of ultrasound combined with CT in the diagnosis and treatment of distal ureteral calculi.

Clinical data of 103 patients with ureteral calculi who received diagnosis and surgical treatment in Benniu People's Hospital, Xinnorth District, Changzhou City from January 2022 to May 2024 were retrospectively analyzed. Ultrasonography and CT exploration were performed on all patients before surgery, and the single diagnosis and combined diagnosis of ultrasonography and CT as well as the location of calculi detected by operation and pathology were analyzed. The consistency of single diagnosis and combined diagnosis of ultrasound and CT with surgical pathological findings was calculated, and the efficacy of single or combined diagnosis of ultrasound and CT in the diagnosis of distal ureteral calculi was calculated using surgical pathological results as the gold standard. At the same time, all patients were re-examined by ultrasound and CT one week after surgery to observe the effect of stone removal.

According to kappa consistency test, the consistency of single ultrasound diagnosis and surgical pathological results was moderate (Kappa=0.607), the single CT diagnosis and surgical pathological results were highly consistent (Kappa=0.657), and the diagnosis of ultrasound combined with CT was completely consistent with surgical pathological results (Kappa=0.947). After calculation, the accuracy, sensitivity and specificity of CT single diagnosis of ureteral calculi were higher than those of ultrasound single diagnosis, but there was no significant difference between the two groups (P>0.05). The accuracy and sensitivity of ultrasound combined with CT in the diagnosis of distal ureteral calculi were significantly higher than those of ultrasound and CT alone (P<0.05). The specificity of ultrasound combined with CT in the diagnosis of distal ureteral calculi was higher than that of ultrasound and CT alone, but there was no significant difference between the two groups (P>0.05). One week after operation, 103 patients with ureteral calculi diagnosed by surgery and pathology were examined by ultrasound. The strong echo mass in the ureter disappeared before operation, and ureteral dilatation and intraluminal liquid echo were not detected. One week after operation, the CT scan of 103 patients showed that the high-density focus in the urinary tract and ureteral dilatation signs disappeared, and no hydronephrosis and obstructive renal parenchyma were detected. According to the results of ultrasound and CT exploration, the stone clearance rate was 100%.

Ultrasound and CT are effective in the diagnosis of distal ureteral calculi. The combined diagnosis of the two imaging techniques can further improve the accuracy and sensitivity of the diagnosis, reduce the occurrence of missed diagnosis, and evaluate the stone clearance by re examination of ultrasound and CT after operation, so as to provide effective image data support for clinical diagnosis and treatment.

To evaluate the clinical safety and effecacy of 3D video endoscope and traditional 2D endoscope in thoracoabdominal surgery.

Relevant literatures were retrieved from databases, including PubMed, EMBASE and CNKI. The safety and efficacy of 3D video and 2D endoscopy in the thoracoabdominal cavity were collected. The Newcastle-Ottawa Scale (NOS) was used to evaluate the quality of the included studies, and meta-analysis was performed with RevMan 5.4 software.

66 articles were included, covering 6867 patients. The results of meta-analysis indicate that 3D video endoscope can be used for imaging and operation of thoracic and abdominal surgery. Compared with the traditional 2D endoscope, 3D video endoscope can significantly shorten the operation time [MD=-12.70, 95%CI(-17.13～-8.27)], reduce blood loss [MD=-10.87, 95%CI(-5.48～-6.25)], and shorten the length of hospital stay [MD=-0.39, 95%CI(--0.55～-0.24)]. It also significantly reduced the incidence of complications [OR=0.76, 95%CI(0.66-0.87)].

3D video endoscopy, which combines 3D imaging and electronic endoscopy technology, can effectively shorten the time of thoracic laparoscopic surgery, reduce blood loss and hospital stay, reduce the risk of postoperative complications, and provide a better technical choice for thoracoabdominal surgery.