Full
Outline
To evaluate the diagnostic value of multiplex PCR for detecting common pathogens in children with respiratory infections.
We collected throat swab samples from 265 children with respiratory infections admitted to Tongshan Maternal and Child Health Hospital in Xuzhou between May 2023 and May 2024. These samples were tested using both multiplex PCR and direct immunofluorescence assay (DFA). Analyze the differences in detection results between multiplex PCR and DFA for respiratory pathogens.
For the 265 pediatric samples, multiplex PCR detected 98 positive cases and 167 negative cases, while DFA identified 81 positive cases and 184 negative cases. The number of positive cases detected by multiplex PCR was significantly higher than those detected by DFA, with a statistically significant difference (P<0.05). The detection results for RSV、FluA and FluB showed high consistency between the two methods. In contrast, the consistency of detection for ADV、PIV1 and PIV3 was ordinary. Multiplex PCR demonstrates superior sensitivity, specificity, and accuracy compared to DFA for the detection of RSV, ADV, FluA, FluB, HPIV1, HPIV2, and HPIV3. ROC analysis demonstrated that multiplex PCR exhibited superior diagnostic performance for respiratory pathogens compared to DFA (P<0.05). The AUC for multiplex PCR was 0.981, whereas for DFA, it was 0.799.
Multiplex PCR enables the simultaneous detection of multiple pathogens with high diagnostic efficiency, which is crucial for accurately diagnosing common respiratory infections in children.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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