To assess the presence of enteroviruses and diarrheal viruses in the environmental sewage of Aba in 2022, and to provide evidence supporting effective control measures against these viruses.

Sewage samples were collected monthly throughout 2022, then eluted and concentrated by anionic membrane adsorption.Through this, enteroviruses were isolated and identified. At the same time, nucleic acid was extracted from the concentrated liquid, and real-time fluorescent PCR was used to detect several diarrhea viruses, such as group A rotavirus, norovirus, Sapovirus, human astrovirus and enteric adenovirus.

A total of 12 environmental sewage samples were collected from January to December 2022. Out of these, 3 strains of polioviruses and 9 non-polio enteroviruses were isolated. All 3 poliovirus strains were detected as Type 3 vaccine-like poliovirus (PV) strains. Group A rotavirus and norovirus genotype II were detected every month. Norovirus type I and enteric adenovirus were observed in all months except November. Human astrovirus was absent in samples from July, August, November and December. Sapovirus was not identified throughout the year.

Enteroviruses and diarrheal viruses exist widely in Aba’s environmental sewage. Environmental sewage surveillance can offer a comprehensive view of these viruses’ etiological and epidemiological characteristics. Continuous environmental sewage surveillance can predict and warn the epidemic and outbreak of enteroviruses and diarrheal virus-related diseases.

To analyze the participation of mutual help and pension and influencing factors of the elderly in China, and to provide a reference basis for the long -term development of mutual help.

Based on the China Longitudinal Aging Social Survey (CLASS) data, single -factor deck analysis, non-conditional logistic regression and Shapley decomposition method was used to analyze the influencing factors and differences in the participation of the elderly people in my country.

The participation rate of mutual support for the elderly in China was only 19.72%, the participation level was low, and the awareness of mutual support for the elderly was weak. Multivariate Logistic regression results showed that the participation of the elderly in mutual support for themwas affected by the demographic characteristics, personal physical conditions, family conditions, pension concepts and ways. Shapley decomposition showed that the contribution rate of each factor to the elderly’s participation in mutual support for the elderly was different, and the physical condition of the elderly had the greatest impact on their participation in mutual support for the elderly, the contribution rate was 19.96%. The second was the participation in insurance and the need for help from others, with the contribution rates of 19.27% and 12.33% respectively.

It’s of great importance tocreate a platform for mutual assistance for the elderly, improve the government incentive mechanism, improve the community support network, and create a good atmosphere of mutual assistance, strengthen mutual assistance pension and education, and play a role of family support.

The primary objective of this study was to investigate the potential correlation between the presence of 22 metals in urine and the risk of developing gallstones in Bouyei population in qiannan region, ultimately aiming to furnish a scientific foundation for early preventative measures and targeted interventions.

From July 2018 to August 2019, a baseline survey was conducted among residents aged 30 to 79 years in Qiannan Bouyei and Miao Autonomous Prefecture using multistage stratified cluster sampling, including questionnaires, physical examination and ICP-MS to detect urine metal concentration. Urinary metal concentrations were divided into four groups (Q1, Q2, Q3, Q4), and binary logistic regression model was used to analyze the relationship between single metal and gallstone risk. At the same time, the dose-response relationship between metal concentration and gallstone risk was investigated by using restricted cubic splines.

The gallstone detection rate of Bouyei population was 8.825%. After adjusting for covariates, binary logistic regression analysis revealed that in the male group, compared to the Q1 group, there was a positive association between gallstone risk and selenium in the Q2 group (OR=1.894, 95%CI: 1.046-3.431), Se in the Q3 group (OR=1.912, 95%CI: 1.053-3.472), and Fe in the Q3 group (OR=1.827, 95%CI: 1.040-3.210). In the female group, Al in the Q3 group (OR=1.460, 95%CI: 1.050-2.028) was positively associated with gallstone risk, while Na in the Q4 group (OR=0.654, 95%CI: 0.452-0.948) was negatively associated with gallstone risk, with a dose-response relationship observed.

There is a significant correlation between the concentration of certain metals in urine and the risk of gallstones. In the Bouyei female population in particular, urinary aluminum and urinary sodium concentrations have a significant impact on gallstone formation, providing possible directions for future prevention and treatment strategies.

To construct a land use regression (LUR) model for particulate matter with an aerodynamic diameter ≤2.5 μm (PM2.5) in Zunyi.

Collect and organize hourly PM2.5 monitoring data from routine air quality monitoring stations from January 1, 2020 to December 31, 2020, as well as geographic information data such as land use, roads, population density, altitude, and meteorological factors in Zunyi. Conduct multiple stepwise linear regression with PM2.5 concentration as the dependent variable and geographic information variables and meteorological factors as independent variables to construct a model. Use leave-one-out cross validation (LOOCV) to verify the performance of the model.

The model formula is Y=72.656-0.026×altitude-0.009×NDVI-0.62×average temperature-383.512×average precipitation. The model had R²=0.74, adjusted R²=0.72, cross validation R²=0.69, RMSE=3.51, and a high spatial resolution (250 m×250 m).

The model fits well and fully reflects the influencing factors of PM2.5 spatial distribution in Zunyi. It can be used to estimate individual exposure levels and provide strong support for environmental health epidemiological research.

To explore the association between the accumulation of cardiovascular risk factors and the elevated resting heart rate in community residents aged 35-75 years in Shanghai

Participates were obtained from the China PEACE Million Persons Projects. 8 districts in Shanghai were selected from 2017 to 2019, and the screening was carried out between adults aged 35 to 75 years in communities. Questionnaires, physical examinations and laboratory tests were conducted. The Kruskal-Wallis test and Cochran-Mantel-Haenszel test were used to compare groups by using R 4.2.2 software. Multivariate logistic regression was performed to explore the correlation between the number of common risk factors for cardiovascular diseases, the combination of different risk factors and elevated resting heart rate.

Among 87934 participants, the mean age was 59.1±9.7 years. Compared with the group with no risk factors, the OR(95%CI) for elevated resting heart rate for the groups with 1, 2, 3 and ≥4 risk factor were 1.42（1.36-1.47）、1.70（1.53-1.77）、1.94（1.84-2.05）and 2.17（1.99-2.36）, respectively. The risk of elevated RHR was the most strongly influenced by the cardiovascular risk factor clustering number in the female group aged 35 to 44 with ≥4 risk factors,and the OR(95%CI) was 3.77 (2.10-4.27). In the combination of risk factors, more metabolic risk factors were associated with a higher risk of elevated resting heart rate.

Resting heart rate may serve as a simple and practical indicator of the cumulative effect of multiple cardiovascular risk factors, which can help early identification of individuals at high cardiovascular risk in relatively healthy people, so as to enable early intervention.

To understand the status of alexithymia in people living with HIV aged 50 years and over, and to analyze its influencing factors.

From November 2023 to March 2024, HIV-infected people aged 50 and over in Luzhou City, Ziyang City and Zigong City of Sichuan Province were selected by convenience sampling method. The 20-item Toronto Alexithymia Scale(TAS-20), Loneliness Scale(UCAL) and Montreal Cognitive Assessment Scale(MoCA) were used to conduct a questionnaire survey on people living with HIV aged 50 and over. T-test and ANOVA were used to compare the scores of alexithymia in HIV-infected people aged 50 years and above with different characteristics. Multiple linear regression analysis was used to explore the influencing factors of alexithymia in HIV-infected people aged 50 years and above.

The average scores of TAS-20, UCAL and MoCA were (61.93±10.99) points, (44.91±9.87) points and (18.56±4.86) points, respectively, in 207 HIV-infected patients aged 50 years and above. The detection rate of Alexithymia was 52.2%. The results of multivariate analysis showed that non-marriage (β=0.13,95%CI: 0.03-0.53), loneliness (β=0.44, 95%CI: 0.32-0.55), poor cognitive function (β=-0.25, 95%CI: -0.37-0.14) may be the influencing factor of alexithymia in HIV-infected persons aged 50 years and above.

Compared with other age groups, HIV-infected people aged 50 and above have a higher prevalence of alexithymia, and AIDS prevention and control managers should focus on unmarried, lonely and poor cognitive performance of infected people, to develop personalized interventions for them to alleviate alexithymia.

To explore the causal relationship between testosterone and endometriosis, andto identify and quantify the role of gut microbiota as a potential mediator through Mendelian randomization analysis.

Utilizing the summary data from the IEU GWAS database for testosterone (n=199 569) and endometriosis (8 288 cases and 68 969 controls) as the exposure and outcome variables, and the aggregated gut microbiota data from the MiBioGen Consortium (n=18 340) as the mediator, a bidirectional, two-step Mendelian randomization analysis was conducted. Causal relationships were assessed using inverse varianceweighted, MR-Egger, weighted median, weighted model, and simple modelto assess causality, while sensitivity analysis was conducted to ensure the robustness of the findings.

Impact of testosterone on endometriosis:Inverse variance weighted estimates suggested that testosterone had negative causal effects on endometriosis (OR=0.804, 95%CI:0.670-0.964, P=0.018).Impact of endometriosis on testosterone:Reverse Mendelian randomization showed that endometriosis did not present a causal effect on testosterone (OR=0.991, 95%CI:0.958-1.026, P=0.616).The class Melainabacteria, one of the gut microbiotas, accounted for a 17.21% reduction in the risk of endometriosis associated with testosterone (mediation proportion: 17.21%, 95%CI:0.090-0.264, P=0.035).

Testosterone is one of the protective factors of endometriosis.Low testosterone levels increase the risk of endometriosis.Gut microbiota may be a mediator of the causal effect of testosterone on endometriosis.

To explore the differences in the status of co-morbid combinations and health-related quality of life between urban and rural middle-aged and elderly people, and to provide a reference for improving the health of middle-aged and elderly people with co-morbid combinations of chronic diseases.

A total of 6 481 middle-aged and elderly people aged ≥50 years with at least two chronic diseases in China Healthy Aging and Reproductive Lifestyle Survey (CHARLS) 2020 were selected. The basic characteristics of urban and rural chronic disease co-morbid middle-aged and elderly people were described, the health-related quality of life was evaluated using the health utility value of EQ-5D-3L, and the combination of urban and rural chronic disease co-morbid combinations was analyzed using the association network diagram and Apriori association rule.

The health utility value of the elderly in urban chronic disease co-morbidities was 0.8532±0.2400, which was higher than that of the elderly in rural chronic disease co-morbidities (0.8409±0.1952). The top five urban strong link chronic disease co-morbidity combination weights were: hypertension+dyslipidemia; hypertension+heart disease; hypertension+arthritis; gastric disease+arthritis; dyslipidemia+heart disease. In rural areas, there were: gastric disease+arthritis; hypertension+arthritis; hypertension+dyslipidemia; and hypertension+gastric disease. Chronic disease co-morbidities in rural middle-aged and elderly people produced 105 association rules and 86 association rules in urban. Among the top 10 association rules in terms of strength of association rules, there were 9 association rules for hypertension and 1 association rule for chronic lung disease in the rural posterior, while there were 6 association rules for hypertension, 3 association rules for dyslipidemia and 1 association rule for chronic lung disease in the urban posterior.

Health related quality of life of elderly people with chronic disease co-morbidities was higher in urban than in rural areas, with emphasis on pain management and depression and anxiety management in elderly people with chronic disease co-morbidities. There are differences in the combination of chronic disease co-morbidities in urban and rural areas, and the binary, ternary, and quaternary co-morbidity associations between common chronic disease illnesses are high, and hypertension and other chronic diseases and co-morbidities are the highest, and attention should be paid to preventive treatment of hypertension.

To analyze herpes zoster vaccine (HZV) vaccination intention and influencing factors among people aged 40 years and above.

A stratified sampling method was used to select people aged ≥40 years from 6 streets of 3 districts in Nanjing for a questionnaire survey. Multivariate Logistic regression model was used to analyze the influencing factors of HZV vaccination intention.

Among those who had not been vaccinated with HZV, 27.00% (385/1 426) were willing to receive HZV vaccination. High income region (OR=1.53,95%CI:1.05-2.21), employee basic medical insurance (OR=1.52,95%CI:1.02-2.27), per capita monthly household income >10 000 yuan (OR=2.15,95%CI:1.26-3.67), currently or before retirement engaged in health care (OR=1.86,95%CI:1.05-3.30), self-assessed good or very good health status (OR=3.27,95%CI:1.50-7.09), family history of HZ (OR=1.93,95%CI:1.37-2.71), influenza vaccine immunization history (OR=2.53,95%CI:1.66-3.84), HZV willingness to pay >1 000 yuan (OR=2.49,95%CI:1.44-4.30), perceived susceptibility (OR=1.17,95%CI:1.02-1.34), and self-efficacy (OR=1.59,95%CI:1.28-1.96) were the influencing factors to promote the HZV vaccination intention.

The willingness rate of HZV vaccination in people ≥40 years old in Nanjing is low. Health education about Herpes zoster (HZ) and HZV should be strengthened, especially the susceptibility of HZ and the safety of HZV. According to the characteristics of HZV vaccination recipients, vaccination agencies can take various ways to publicize vaccination service information and optimize the vaccination process such as appointment and payment. Relevant departments should explore HZV immunization strategies to reduce the economic burden of HZV vaccination.

To explore the influence of social networks on health-related behaviors in stroke patients.

From July to September 2023, participants living in Guangdong were selected as the survey respondents using convenient sampling method. The general information questionnaire, stroke health-related behavior questionnaire and social network questionnaire were used to collect data. Social network analysis was used to explore network characteristics and draw social network diagrams. Latent class analysis was used to explore health-related behavior patterns of stroke. Kruskal-Wallis H test and Bonferroni test were used to compare the differences in social networks of different health-related behavior patterns.

Stroke health-related behaviors can be divided into three categories, namely "good health behavior group" (C1, 58.15%), "smoking and drinking group" (C2, 24.60%), and "sedentary lifestyle and imbalanced diet and sleep group" (C3, 17.25%). The network effective size, efficiency, and betweenness centrality of the "smoking and drinking group" and the "sedentary lifestyle and imbalanced diet and sleep group” were lower than that of the "good health behavior group" (P<0.05/3). In contrast, the density, network hierarchy, network constraint, percentage of individuals who smoked and drank, and percentage of individuals who did not exercise and ate healthily of the two groups were higher than that of the "good health behavior group" (P<0.05/3).

Social network characteristics with high betweenness centrality, low hierarchy, low constraint and high network efficiency can promote health-related behaviors of stroke. Building a social network with equality, low constraint and positive healthy interaction is helpful for community stroke prevention and treatment.