To assess the presence of enteroviruses and diarrheal viruses in the environmental sewage of Aba in 2022, and to provide evidence supporting effective control measures against these viruses.
Sewage samples were collected monthly throughout 2022, then eluted and concentrated by anionic membrane adsorption.Through this, enteroviruses were isolated and identified. At the same time, nucleic acid was extracted from the concentrated liquid, and real-time fluorescent PCR was used to detect several diarrhea viruses, such as group A rotavirus, norovirus, Sapovirus, human astrovirus and enteric adenovirus.
A total of 12 environmental sewage samples were collected from January to December 2022. Out of these, 3 strains of polioviruses and 9 non-polio enteroviruses were isolated. All 3 poliovirus strains were detected as Type 3 vaccine-like poliovirus (PV) strains. Group A rotavirus and norovirus genotype II were detected every month. Norovirus type I and enteric adenovirus were observed in all months except November. Human astrovirus was absent in samples from July, August, November and December. Sapovirus was not identified throughout the year.
Enteroviruses and diarrheal viruses exist widely in Aba’s environmental sewage. Environmental sewage surveillance can offer a comprehensive view of these viruses’ etiological and epidemiological characteristics. Continuous environmental sewage surveillance can predict and warn the epidemic and outbreak of enteroviruses and diarrheal virus-related diseases.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |