To construct a Propionibacterium acnes (P. acnes)-induced cellular inflammation model and to study the anti-inflammatory effect of a thermal water.

Utilize human immortalized keratinocytes (HaCaT) was used in the experimentation. Co-incubate the cells with thermal spring water and employ the CCK-8 assay to assess cell viability, screening for the optimal intervention time for the thermal spring water. Expose the cells to varying concentrations of P. acnes for different times, measure cell viability, calculate the 50% inhibitory concentration (IC₅₀) of P. acnes, and determine the model induction conditions by testing IL-6 levels. The cells were soaked in thermal spring water for 0.5 hours, followed by co-culturing with P. acnes as the preventive intervention group, or co-culturing with P. acnes first and then soaking the cells in hot spring water for 0.5 hours as the therapeutic intervention group. The levels of cell viability, pro-inflammatory cytokines, and gene expression of inflammation pathways were assessed using the CCK-8 assay, ELISA, and RT-PCR, respectively. Concurrently, control groups with saline solution, tap water, and pure water, along with model and blank controlswere set up.

After treating the cells with thermal spring water and physiological saline for 0.5 hours, cell viabilities were 92.7% and 99.09%, respectively, which led to the selection of 0.5 hours as the intervention time for subsequent experiments.P. acnes at an OD₆₀₀ of 0.25 was co-incubated with the cells for 5 hours to establish the model. In the preventive intervention, the secretion levels of IL-1β and IL-8， as well as the relative expression levels of IL-6, TNF-α, IL-8, and the genes TLR2 and NF-κB in the cells of the thermal spring water group, were all lower than those in the model group (P<0.05), and significantly lower than those in the saline solution group. In the therapeutic intervention, the contents of IL-6, IL-1β, TNF-α, and IL-8, as well as the expression levels of the genes IL-6, IL-1β, TNF-α, TLR2, and NF-κB in the thermal spring water group, were all lower than those in the model group. The contents of IL-6, IL-1β, TNF-α, and the expression levels of the genes IL-6, IL-1β, IL-8, TLR2, and NF-κB were also significantly reduced compared to those in the saline solution group (P<0.05).

This thermal water may exert its anti-inflammatory effect by down-regulating the expression of TLR2 and NF-κB genes in inflammatory signalling pathway and by reducing the secretion of pro-inflammatory cytokines.

To analyze the influencing factors of preventive service cost in Guizhou Province from 2016 to 2022 by using grey correlation degree method.

Based on the accounting results of “SHA2011” and the data of “Guizhou Statistical Yearbook” and “Guizhou Health Statistical Yearbook”, the factors affecting the cost of preventive services in Guizhou from 2016 to 2022 were analyzed by using grey correlation method.

The highest correlation with the cost of preventive services in Guizhou province was the number of health technicians in epidemic prevention and control and maternal and child health institutions (0.826), followed by the gross enrollment rate of higher education (0.735), the number of health technology beds in epidemic prevention and control and maternal and child health institutions (0.730). The lowest correlation was the proportion of tertiary industry in GDP (0.593).

Health resource input has the strongest correlation with the cost of preventive services. Health fund input and public health services are the main influencing factors of the cost of preventive services in Guizhou Province. At the same time, full attention should be paid to the influence of social factors such as economic development, education level, urbanization level and age structure on the cost of preventive services.

To explore the current situation and influencing factors of medical students’ sleep quality, and to provide targeted intervention measures for improving sleep quality.

A questionnaire survey was conducted among 2884 medical students from September 2021 to January 2022 using a convenience sampling method. Multiple correspondence analysis and multivariate logistic regression analysis were used to explore the influencing factors of medical students’ sleep quality.

The prevalence of poor sleep quality among medical students was 70.32%. The results of multiple correspondence analysis showed that female medical students, sophomores, those with poor self-rated interpersonal relationships and poor health-promoting lifestyles were more likely to have poor sleep quality. The results of multiple stepwise logistic regression analyses showed that four independent variables entered the regression equation, gender (OR_female=1.34,95% CI: 1.13-1.60), grade (OR_sophomore=2.23,95% CI: 1.72-2.88), self-rated interpersonal relationships (OR_poor=1.76,95% CI: 1.47-2.12) and health-promoting lifestyle (OR_poor=2.02, 95% CI: 1.70-2.39).

The prevalence of poor sleep quality among medical students is at a high level. There is a need to focus on sophomores and female students, and to take targeted interventions from the aspects of interpersonal relationship improvement and healthy lifestyle development to improve sleep quality.

To analyze the chain-mediated roles of intergenerational spiritual support and depression between social participation and cognitive functioning in older adults.

In this paper, we used data from the China Health and Aging Tracking Survey (CHARLS) 2020 to screen 3 737 study participants who met the criteria. Depression and cognitive function were measured using the Center for Streaming Depression Scale (CES-D 10) and the Overall Cognitive Scoring Instrument, respectively. Two-column correlation analyses (Pearson’s correlation analyses) of correlation were used, and multiple mediating effect models were constructed to investigate the mediating effects of intergenerational spiritual support and depression in the impact of social participation activities and cognitive functioning in older adults.

After including control variables such as age, gender, marital status, chronic disease prevalence, and insurance participation, regression analysis showed that older adults’ social participation status had a significant direct effect on their cognitive functioning (β=0.254, P<0.001), while intergenerational spiritual support (β=0.052, P<0.050) and depression status (β= -0.084, P<0.001) also had a significant effect on cognitive function in older adults. The results of mediation effect analysis showed that the simple mediation effects of intergenerational psychiatric support and depression status on the relationship between social participation status and cognitive function in older adults were 0.006(95% CI: 0.001-0.012) and 0.020(95% CI: 0.006-0.036), respectively, and the chained mediation effect was 0.001(95% CI: 0.001-0.004).

This study finds a direct mediating effect of depressive status and intergenerational psychiatric support between social participation status and cognitive functioning in older adults, as well as a chain-mediated effect between the two. Social participation of older adults should be improved, while their cognitive function can be improved by children caring more about their parents and reducing depressive symptoms to delay or prevent the occurrence of cognitive impairment in older adults.

To analyze the characteristics of Adverse Events Following Immunization (AEFI) of domestic rotavirus (live) vaccine (RVVO) in Shaanxi province from 2018 to 2022, and to evaluate the safety of RVVO vaccination.

The AEFI case data on RVVO vaccination in Shaanxi Province between 2018 and 2022 was gathered from the China AEFI Information Management System., and the vaccination situation of RVVO in Shaanxi province was collected through the immunization planning information management system of Shaanxi province. The analysis was conducted using the descriptive epidemiological technique.

A total of 461 102 doses of RVVO were inoculated in Shaanxi province from 2018 to 2022, and 48 cases of AEFI were recorded, with an incidence of 10.41 cases per 100 000 doses, including 43 cases of normal reactions (9.33 cases per 100 000 doses) and 3 cases of abnormal reactions (0.65 cases per 100 000 doses). 2 cases of coupling disease and No serious AEFI cases recorded. The three abnormal reactions were allergic rash (0.22/100 000 doses), acute diarrhea (0.22/100 000 doses) and rotavirus diarrhea (0.22/100 000 doses). 97.92% of the reported AEFI cases were cured or improved after follow-up, and no sequelae and death cases were reported. The age of onset of AEFI was less than 1 year old and occurred on the day of inoculation. The incidence of AEFI at the first, second and third doses was 12.65 cases per 100 000 doses, 10.64 cases per 100,000 doses and 361 cases per 100 000 doses, respectively. RVVO combined with other vaccines reported a general reaction rate of 10.16 per 100 000 doses and an abnormal reaction rate of 0.97 per 100 000 doses in AEFI.

The domestic oral live attenuated rotavirus vaccine has good safety, and the timely vaccination rate of children should be further strengthened.

To explore the current situation and development trend of coupling and coordination of medical resource allocation and service utilization in traditional Chinese medicine hospitals, to clarify the coordinated development relationship between the two, and to provide reference opinions for promoting the benign interaction between the two and achieving balanced development.

From 2017 to 2021, 11 relevant index data of medical resource allocation and service utilization of TCM hospitals in 30 provinces of China were selected, and the comprehensive development index and relative development degree were used to comprehensively evaluate the allocation and service utilization of TCM hospitals, and the coupling coordination method was used to explore the coupling and coordination relationship and development trend of the two systems.

From 2017 to 2021, the coupling coordination degree of medical resource allocation and service utilization of TCM hospitals in various regions of China decreased, showing a stepwise decreasing pattern from east to west. There were obvious differences in the comprehensive development level of medical resource allocation of traditional Chinese medicine hospitals among regions, and the lag in medical resource allocation had become a constraint for the coordinated development of the western region. The overall structure of the relative development level had changed from “8-13-9” in 2017 to “8-12-10” in 2021, and only 12 provinces would achieve coordinated development.

There is a lot of room for improvement in the coupling and coordination of resource allocation and service utilization of traditional Chinese medicine hospitals in China, and the allocation level of medical resources in traditional Chinese medicine hospitals should be optimized according to local conditions, so as to promote the high-quality development of traditional Chinese medicine hospitals with economy. The improvement strategy of coupling coordination level is dynamically adjusted according to the relative development degree, so as to promote the benign interaction between the two and achieve high-quality coordinated development.

To explore the relationship between the dynamics of plant-based diets and mortality in Chinese older adults.

Based on the data from the Chinese Longitudinal Healthy Longevity and Happy Family Study (CLHLS), older adults who participated in the three surveys from 2011-2018 were selected. The HPDI and UPDI scores of the study participants were calculated in 2011 and 2014, respectively, and they were categorized into four groups based on the median of their two scores: low-low, low-high, high-low and high-high groups. Cox proportional risk regression models were used to analyze the relationship between the dynamics of plant-based diets and mortality in the elderly.

A total of 4 382 subjects were enrolled in this study, and 1 689 (38.5%) of them died during the 7-year follow-up period. The results of Cox proportional hazards regression model showed that after adjusting for confounding factors such as gender, age, and education, the risk of death in the high-high HPDI group was reduced by 17% (HR=0.83, 95% CI: 0.72-0.97), while the risk of death in the high-high UPDI group was increased by 29% (HR=1.29, 95% CI: 1.11-1.49).

Older adults who maintain a healthy plant-based diet have a lower risk of death.

To grasp the current situation and trends of occupational hazards in workplaces of key industries in Ji’an City, and provide a basis for improving the prevention and control of occupational diseases.

The data of occupational hazards in the workplaces of key industries in Ji’an City from 2021 to 2023 were collected from the Workplace Occupational Hazard Monitoring System, and the monitoring results were summarized and analyzed.

A total of 654 enterprises from key industries were recruited as the monitoring subjects in Ji’an City from 2021 to 2023, which were mainly small and micro-scale(94.34%) and manufacturing (74.92%) industries. The exposure ratio of occupational hazards among employees was 55.35%, and the exposure ratio of noise hazard was the highest(47.44%), the second was dust hazard (44.12%). The rates of occupational health training for enterprise leaders, occupational health management personnel, and workers were 72.78%, 72.78% and 83.74%, respectively, which were all increasing gradually year by year (χ²=15.808, 17.649, 398.746, respectively, all P<0.05). The distribution rates of dust masks, anti-poisoning masks or face masks, and noise prevention earplugs or earmuffs were 90.39%, 61.33% and 66.31%, respectively. The distribution rates of dust masks and noise prevention earplugs or earmuffs showed an increasing trend year by year (χ²=24.010, 21.100, respectively, all P<0.05). The rates of installation of dust prevention facilities, anti-poisoning facilities and noise prevention facilities were 88.99%, 76.00% and 54.67%, respectively, which were all increasing year by year (χ²=14.625, 9.818, 34.993, respectively, all P<0.05). There were 437 enterprises exceeded the standard, with an exceeding rate of 66.82%. From 2021 to 2023, the exceeding standard rates of post testing were 40.92%, 24.41%, 24.17%, respectively, which showed a decrease trend year by year (χ²=78.647, P<0.05), and increased with the decrease of enterprise size (χ²=10.093, P<0.05). Among the different kinds of occupational hazard factors, the exceeding rate of noise posts was the highest, the exceeding rates of dust posts and chemical agents’ posts showed a decrease trend year by year (χ²=72.219, 31.342, respectively, all P<0.05).

In recent years, the status of occupational disease hazards in key industries in Ji’an City has been improved, but the situation is still very serious. It is necessary to further strengthen and improve the prevention and control of occupational diseases to protect the occupational health rights and interests of workers.

To observe the effects of breastfeeding on intestinal flora and short-chain fatty acids (SCFAs) in infants and how they affect the occurrence of infant allergies.

A nested case-control study was conducted in an existing mother-infant cohort. A total of 48 infants were selected as study subjects and divided into a healthy/allergic mixed feeding group and a breastfeeding group. Fecal samples were collected at 15 days, 1 month, 6 months, and 12 months. The contents of three SCFAs in the samples were determined by gas chromatography-mass spectrometry (GC-MS), and the bifidobacteria in infant feces were quantitatively analyzed by real-time fluorescent quantitative PCR.

Feeding patterns mainly affected bifidobacteria at 15 days and 1 month. The bifidobacteria level in healthy mixed-fed infants was significantly higher than that in allergic mixed-fed infants at 15 days (7.75±0.60 CFU/g vs 6.28±0.28 CFU/g, t=2.232, P=0.047). At 1 month, the bifidobacteria level in healthy mixed-fed infants was higher than that in healthy breastfed infants (8.01±0.50 CFU/g vs 6.62±0.13 CFU/g, t=2.712, P=0.027). There were differences in the main SCFAs between healthy and allergic infants with different feeding patterns. On day 15, the acetic acid content in the healthy breastfeeding group was significantly higher than that in the allergic breastfeeding group and the healthy mixed feeding group (1 035.10 μg/g vs 285.55±90.64 μg/g, t=3.698, P=0.034; 1 035.10 μg/g vs 406.11±63.19 μg/g, t=3.148, P=0.014), and the propionic acid content in the healthy mixed feeding group was higher than that in the allergic mixed feeding group (128.00±32.24 μg/g vs 12.61±3.90 μg/g, t=3.553, P=0.007). At 6 and 12 months, the propionic acid content in the healthy mixed feeding group was lower than that in the allergic mixed feeding group. There were varying degrees of correlation between SCFAs and bifidobacteria in the intestines of healthy and allergic infants with different feeding patterns. In the healthy group, a simple and stable relationship between bifidobacteria and SCFAs was observed in mixed-fed infants, while in the allergic group, the relationship between SCFAs and bifidobacteria was complex and had no obvious pattern.

Feeding patterns affect the occurrence of infant allergies by influencing intestinal flora and SCFAs in infants. Mixed-fed infants are more prone to allergies, while breastfeeding has a significant protective effect on the intestines of allergic infants.

To understand the current status of psychological therapy and counseling services in mental health institutions in mainland China so as to provide reference basis for developing subsequent relevant policies.

From May to June 2021, the National Mental Health Project Office conducted a survey using a self-designed questionnaire on 3 833 institutions providing psychological therapy and counseling services in 31 provinces in mainland China. The data was collected by the mental health project offices at all levels and submitted to the National Mental Health Project Office.

2 214(57.76%) institutions provided psychological therapy or counseling services to both outpatient and inpatient patients. In 2020, we served over 21.86 million people, with outpatient patients slightly higher than inpatients. 2 685(70.05%) institutions charge fees, of which 1 206(44.92%) reported that their psychological therapy services are included in medical insurance. The fee standards for psychological therapy and counseling services vary greatly among different institutions, with psychological therapy services ranging from 5 yuan RMB to 3 000 yuan RMB per session and psychological counseling services ranging from 5 yuan RMB to 2 400 yuan RMB per session.

Residents have low utilization of psychological therapy or counseling services within medical institutions. The prices of psychological therapy or counseling services vary greatly, and the medical security needs to be deeply implemented.