To investigate the mediating effects of daily activity ability and depression on pain status and subjective life expectancy in elderly patients with chronic diseases, and to provide suggestions and references for active aging.

Based on The China Health and Retirement Longitudinal Study (CHARLS) 2020 data, 5 768 patients with chronic diseases aged ≥60 years were selected as the study subjects, and Spearman correlation analysis was used to explore the correlation between pain, ability to perform activities of daily living, depression, and subjective life expectancy, and PROCESS 4.1 program Model 6 was used to construct the chain-mediated model.

Pain was positively correlated with impaired daily activity ability and depression (r=0.399, P<0.001; r=0.370, P<0.001), was negatively correlated with subjective life expectancy (r=-0.221, P<0.001); The mediating effects of daily activity ability and depression on pain and subjective life expectancy were significant, with simple mediating effects of -0.023 (95%CI: -0.031 to -0.016) and -0.042 (95%CI: -0.049 to -0.034), respectively. The chain mediation effect was -0.013(95%CI: -0.016 to -0.010). The total mediating effect of daily activity ability and depression on pain and subjective life expectancy was -0.078, accounting for 57.78%.

There is a chain mediated effect between daily activity ability and depression on pain status and subjective life expectancy in elderly patients with chronic diseases. With the popularization of primary medical and health services, the family doctor service team needs to improve the comprehensive management of elderly patients with chronic diseases, improve the subjective well-being of elderly patients with chronic diseases from pain management, daily activity intervention, depression regulation and other aspects, and prolong the subjective life expectancy.

To explore the relationship between oral hygiene behavior and depression level in elderly people.

This article used the 2018 data from the Chinese Elderly Health Longevity Factors Survey (CLHLS) to select 10 148 eligible research subjects. Pearson correlation analysis was used to explore the correlation between oral hygiene behavior, dietary diversity, life satisfaction, and depression levels in the elderly. The Process4.1 plugin model in SPSS was used to analyze the mediating role of dietary diversity and life satisfaction between oral hygiene behavior and depression levels in the elderly.

The direct impact of oral hygiene behavior on depression levels in the elderly was significant (β=-0.206,P<0.01); Dietary diversity and life satisfaction significantly affected the level of depression in elderly people (β=-0.270, P<0.01); (β=-2.992,P<0.01); The mediating effects of dietary diversity and life satisfaction on the relationship between oral hygiene behavior and depression levels in elderly individuals were-0.069 (95%CI:-0.088 - -0.051) and -0.099 (95%CI:-0.145 - -0.055), respectively, with a chain mediation effect of -0.060 (95%CI:-0.071 - -0.049).

Dietary diversity and life satisfaction have a mediating effect between oral hygiene behavior and depression levels in the elderly, and there is a chain mediating effect between dietary diversity and life satisfaction and depression levels in the elderly.

To explore the potential categories of health literacy of the elderly with chronic disease comorbid disability and their relationship with family nursing quality.

713 comorbid disabled elderly in Urumqi, Xinjiang were investigated by general data, function of daily living index, health literacy scale for chronic disease patients and Family Care Quality Scale for disabled elderly (FCCI). Mplus 8.3 was used to make potential profile analysis of their health literacy, and SPSS 26.0 software was applied. To compare the quality of family care for the elderly with comorbidity and disability in different categories of health literacy.

The health literacy of the elderly with concomitant disabilities can be divided into three categories:high health literacy - high willingness (35.5%), medium health literacy - good acceptance (47.7%), and low health literacy - low access to information (16.8%). There were significant differences in the total score of family care quality and the scores of each dimension among the 3 different health literacy groups (P<0.001). The total score of family care quality in the group with high health literacy and high willingness was the highest (47.47±0.128), and the total score of family care quality in the group with low health literacy and low information access was the lowest (40.28±0.315). The scores of family care quality in the group with high health literacy and high willingness were higher than those in the other two groups, including scores of disabled elderly (28.03±0.053), caregiver (11.02±0.054) and family members (8.42±0.062). The scores of family care quality in the group with low health literacy and low access to information were lower than those in the other two groups, including scores of disabled elderly (24.78±0.176), caregiver (8.97±0.116) and family members (6.53±0.101).

There are three potential categories of health literacy for the elderly with comorbid disabilities, and the quality of family care for the elderly with comorbid disabilities is different in different potential categories. It is suggested that targeted personalized intervention programs should be developed based on the groups with the same health literacy of the elderly with comorbid disabilities, so as to improve the quality of their family care.

To understand the various metal elements content and distribution in crayfish sold in Deyang, and to initial determine the harm of hazardous metallic elements to residents in Deyang.

A total of 102 samples were collected in Deyang using a stratified random sampling approach, the samples was digested by microwave digestion,17 mineral elements and 7 harmful metal elements were detected using inductively coupled plasma optical emission spectroscopy (ICP-OES) and inductively coupled plasma mass spectrometry(ICP-MS).

Mineral elements in all parts of the crayfish were detected and the content varies, in terms of total minerals, crayfish brain> crayfish intestine> crayfish gills> crayfish meat> crayfish hepatopancreas. For harmful metals, there was no exceedance in crayfish meat, As, Cu, Cd in crayfish hepatopancreas exceeded the standard rate of 97.0%, 2.97%, 27.7%, Hg, Pb, Cr did not exceed the standard, As, Cu, Pb, Cr and Al were all exceeded the standard in the non-edible parts by 2.17% to 100%. The enrichment pattern of all metal elements in crayfish in Deyang and Hubei was consistent, but the contents varied. In different sampling sessions, none of the harmful metal elements in crayfish meat exceeded the standard; As, Cu, Cd in crayfish hepatopancreas exceeded the standard rate of 2.8%-97%, and the rest of the elements did not exceed the standard. In different growth stages, except for As, there was no significant difference in the accumulation of hazardous metal elements between green and red crayfish.

Crayfish have a high mineral content and abundant nutrients, and local residents in Deyang City have a lower risk of heavy metal hazards in their daily intake of crayfish.

To investigate the association between biological aging and cognitive function in middle-aged and elderly Chinese.

The subjects were health check-up personnel over 45 years old from a top three hospital. The general demographic characteristics, covariates and cognitive function status were collected by questionnaire and Montreal cognitive assessment (MoCA). Multivariate linear regression and logistic regression were used to analyze biological aging index (BAI) and biological aging type (BAT) association with MoCA score and risk of mild cognitive impairment (MCI). Subgroup analysis was conducted by the middle-aged group (45-64 years old) and the elderly group (65 years old and above) to explore whether the correlation between biological aging and cognitive function was different in different age groups.

A total of 407 participants were included in this study. The average MoCA score was 23.30±4.30, and the detection rate of MCI was 41.5%. BAI was negatively correlated with MoCA score, and the regression coefficient after adjusting for covariates was -0.135 (95%CI: -0.229--0.042). Compared with those with delayed aging, subjects with premature aging had lower cognitive function scores (β=-0.882，95%CI：-1.566--0.198) and a higher risk of MCI (OR=1.736，95%CI：1.072-2.833). Subgroup analysis found that the association was still present in the middle-aged group, but not in the elderly group.

There is a negative correlation between biological aging and cognitive function in middle-aged and elderly people, and the cognitive function of subjects with premature aging is worse. This effect is more significant in middle-aged group, suggesting that individuals with premature aging should be identified in middle age or even earlier and that intervention measures should be taken to prevent or delay the occurrence of dementia.

To establish a method for the determination of 18 phthalates (PAEs) metabolites in urine by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based on solid-phase support liquid-liquid extraction (SLE) technology.

Urine sample spiked with internal standards was first hydrolyzed by enzyme, then purified on a SLE column. The separation was performed on a BEH C18 column. The detection was performed in multi-reaction monitoring scan under negative mode. Internal standard method was applied for quantitative analysis.

The 18 PAEs metabolites showed good linear relationships in the range of 0.10-80 ng/ml (r>0.999). The average recoveries of 18 PAEs metabolites ranged from 84.0%-113.0% with relative standard deviations between 1.1% and 9.5%. The limits of detection and quantitation of the method were 0.001-0.07ng/ml and 0.002-0.2 ng/ml, respectively. The intra-day and the inter-day precisions were 1.3%-7.0% and 1.0%-8.1%, respectively. 160 urine samples were analyzed with this method. Except for 3 PAEs metabolites that were not detected, the detection rates of the remaining 15 PAEs metabolites ranged from 1.9% to 100%, and the detection rates of 8 PAEs metabolites were 100%. Among them, mono-n-butyl phthalate had the highest concentrations with a median concentration of 126 ng/ml.

This method is simple, sensitive, and accurate, and it is suitable for the determination of 18 PAEs metabolites in large batchs of urine samples.

To understand the utilization and demand status of cancer screening services among residents in Sichuan Province, and provide scientific basis for further promoting cancer screening strategies.

Using multi-stage stratified clustering and probability proportional to size (PPS) sampling, a face-to-face questionnaire survey was conducted among 15-74-year-old permanent residents from 10 cities (prefectures) in Sichuan Province. Using chi-square test for univariate analysis of cancer screening service utilization and demand, and using logistic regression model for multivariate analysis.

A total of 7 442 valid questionnaires were collected, with an effective rate of 91.30%. 1 099 residents (14.77%) had undergone cancer screening, aged between 40 and 60 years old (OR=1.62, 95%CI:1.31-2.00), female (OR=1.92, 95%CI:1.65-2.25), married, with a college/undergraduate education or above, civil servants and public institution staff (OR=1.59, 95%CI:1.20-2.09), with a family history of cancer, urban household registration (OR=1.46,95%CI:1.23-1.74), poor self-rated health status, and higher utilization rate of cancer screening services for urban employees with basic medical insurance.5034 residents (67.64%) had a demand for cancer screening services. Married individuals with higher education levels, total household income ≥ 60000 yuan/year (OR=1.20,95%CI:1.07-1.35), family history of cancer, and poor self-rated health status had a higher demand for cancer screening services.

There is a high demand for cancer screening services among residents in Sichuan Province, but the actual utilization is insufficient. It is recommended to improve and promote cancer screening work by strengthening health education and establishing a multi-channel funding mechanism for cancer screening.

To evaluate the associations between circulating monounsaturated fatty acids(cMUFAs) and cardiovascular-specific death using epidemiological methods.

This study included 36 254 patients with cardiovascular disease from the UK Biobank. Cox proportional hazards models were employed to examine the associations between cMUFAs and various fatalities, including cardiovascular death, ischaemic heart disease death, stroke death, cancer death, and all-cause death. Moreover, the study examined the dose-response relationship between cMUFAs and outcomes using quartiles, deciles, and restricted cubic splines. A subgroup analysis was conducted to validate the effects of cMUFAs on cardiovascular-specific deaths across different subgroups.

A positive correlation was observed between cMUFAs and all-cause death (HR=1.556, 95% CI: 1.419-1.705), as well as CVD (HR=1.504, 95% CI: 1.261-1.794), IHD (HR=1.572, 95% CI: 1.265-1.953), and cancer death (HR=1.291, 95% CI: 1.109-1.502). However, no significant association was found between cMUFAs and stroke death (P=0.582). The study revealed the existence of non-linear relationships between cMUFAs and cardiovascular-specific death. Subgroup analyses indicated that the association with all-cause death was more pronounced in females and current smokers. Furthermore, individuals aged over 50 years demonstrated a more pronounced correlation with IHD death.

In patients with cardiovascular disease, elevated levels of cMUFAs are associated with an increased risk of CVD death, IHD death, cancer death, and all-cause death. This association demonstrates a non-linear correlation trend.

To explore the incidence, time of occurrence and influencing factors of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in stable chronic obstructive pulmonary disease (COPD) patients within one year.

Retrospectively collected information on COPD case home page visits in Yunnan Province from 2016 to 2022, establishing a longitudinal observation cohort based on patients’ names and ID numbers. The incidence rate, time of occurrence and average number of AECOPD within one year of COPD were calculated, and the relationship between patients’ exposure factors and the number of AECOPD within one year was analyzed by using multivariate linear regression, and the exposure factors affecting AECOPD within one year of COPD patients were analyzed by using Cox regression.

The mean age of 405 428 COPD patients was（70.75±10.95）years, the mean number of comorbidities was 1.14±1.15, the incidence of AECOPD within one year was 17.55%, the mean duration of occurrence was（4.58±3.64）months, and the mean number of AECOPD per patient was 0.27. The regression results showed that gender (HR=1.473, 95%CI: 1.446-1.500), age (HR=1.011, 95%CI: 1.010-1.012), number of comorbidities (HR=1.083, 95%CI: 1.076-1.090), medical insurance type (HR=1.481, 95%CI: 1.394-1.574), and admission season (HR=1.115, 95%CI: 1.090-1.140) were the influencing factors for acute exacerbation in patients with COPD within one year.

The incidence of AECOPD in COPD patients in Yunnan Province within one year is 17.55%. We should start from various risk factors and focus on male, elderly, patients with multiple comorbidities, those admitted in spring and winter seasons, and non-resident medical insurance patients. Take corresponding preventive measures and strengthen the management of AECOPD.

To investigate the relationship between social alienation and health promotion behaviors in community chronic co-morbid older adults, and explore the mediating role of self-perceived aging.

Using the convenience sampling method, A questionnaire survey was conducted using the General Information Questionnaire, the General Alienation Scale, the Simplified Self-perceived Aging Scale, and the Health Promoting Lifestyle Scale on 611 community-based older adults with chronic co-morbidities in Hangzhou, and structural equation modeling was constructed using AMOS.

The total score of chronic co-morbid social alienation in the community was (36.38±10.41) points, the total score of self-perceived aging was (50.10±14.68) points, and the score of health promotion behavior was (93.79±15.56) points. The results of the mediation effect test showed that the effect value between social alienation and health promotion behavior was -0.205 (95%CI: -0.285 to -0.121), accounting for 45.86% of the total effect; the effect value of self-perceived aging in the mediation effect between social isolation and health promotion behavior was -0.242 (95%CI: -0.299 to -0.191); the total effect value was -0.447 (95%CI: -0.508 to -0.383), and the mediation effect accounted for 54.14% of the total effect.

Social alienation and self-perceived aging of chronic co-morbid older adults directly affect health promotion behaviors, while social alienation can indirectly affect health promotion behaviors through self-perceived aging.