To investigate whether sub chronic exposure to Benz[a]pyrene (BaP) induces ferroptosis in mouse hepatocytes, leading to liver injury, thereby providing a basis for further study of the hepatic toxicity mechanisms of BaP.

Forty-eight male C57BL/6 mice aged three weeks were randomly divided into six groups: control group (corn oil),low-dose BaP group (2.5 mg/kg), medium-dose BaP group (5 mg/kg), high-dose BaP group (10 mg/kg), ferroptosis inhibitor Fer-1 group (3-amino-4-cyclohexylaminobenzoic acid ethyl ester, 1 mg/kg), and high-dose BaP + Fer-1 group (10 mg/kg + 1 mg/kg). BaP was administered via gastric gavage dissolved in corn oil, and Fer-1 was injected intraperitoneally every other day for 90 days. Hepatic structure and collagen fiber deposition were observed using HE and Masson staining, while transmission electron microscopy (TEM) was used to examine the ultrastructure of hepatocytes. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mouse liver tissues, as well as tissue iron, malondialdehyde (MDA), glutathione (GSH) content, and glutathione peroxidase (GSH-Px) activity, were measured using kits. Western blotting was employed to detect the levels of glutathione peroxidase 4 (GPX4) and acyl-CoA synthetase long-chain family member 4 (ACSL4) in mouse liver tissues.

Compared to the control group, the high-dose BaP group exhibited a decrease in body weight (t=4.921, P=0.006) and a reduction in liver coefficient in both medium and high-dose BaP groups (t _medium=4.967, P<0.05; t _high=6.568, P=0.001). The ALT and AST levels in liver tissues of BaP-treated mice were elevated (F _ALT=218.200, P<0.001; F _AST=421.200, P<0.001). HE staining revealed varying degrees of hepatocyte disarray, sinusoidal dilation, congestion, and inflammatory infiltration in the liver of BaP-treated mice, while Masson staining indicated collagen fiber deposition. Iron content in the liver tissues of BaP-treated mice increased (W=41.730, P<0.001), and both GSH concentration and GSH-Px activity decreased (W _GSH=49.640, P<0.001; F _GSH-Px=252.400, P<0.001), with an increase in MDA concentration (F=207.700, P<0.001). The expression level of GPX4 protein in the liver of BaP-treated mice decreased (F=56.790, P<0.001), while the expression level of ACSL4 protein increased (F=429.400, P<0.001). Compared to the high-dose BaP group, the high-dose BaP + Fer-1 group showed improvements in body weight changes (t=5.970, P<0.001), liver coefficient (t=11.510, P<0.001), morphological changes in the liver, and levels of ALT (q=21.730, P<0.001), AST (q=32.870, P<0.001), tissue iron (t=5.045, P=0.009), GSH (t=10.600,P<0.001), GSH-Px (q=9.977, P<0.001), MDA (q=21.580, P<0.001), as well as the expression levels of ACSL4 (q=8.629, P<0.001) and GPX4 (q=5.146, P=0.03) proteins.

Sub chronic exposure to BaP can induce ferroptosis in mouse hepatocytes, resulting in liver injury.

To explore the relationship between parental emotional expression and depression symptoms in adolescents.

A random cluster sampling method was employed to select 13 240 adolescents from Pidu District, Chengdu. A general demographic questionnaire, the Chinese version of the Emotional Expression Scale, and the Chinese version of the Kutcher Adolescent Depression Scale were used for investigation and psychological assessment. Binary logistic regression analysis was conducted to examine the association between parental emotional expression and adolescent depression symptoms, with subgroup analysis based on factors such as gender, region, family economic status, only-child status, and primary caregiver.

The study found that 2 726 adolescents (20.6%) exhibited depression symptoms. Among the participants, 6 697 adolescents (50.6%) reported high levels of parental emotional expression. The incidence of depression symptoms was significantly higher in the group with high parental emotional expression compared to the group with low parental emotional expression (P < 0.001). Logistic regression analysis indicated that high parental emotional expression was a risk factor for depression symptoms in adolescents, even after controlling for multiple confounding factors (OR=2.08, 95%CI: 1.87-2.30). Subgroup analysis did not reveal any interaction between parental emotional expression and factors such as gender, region, or family economic status (P_interaction > 0.05).

Adolescent depression symptoms are closely associated with high parental emotional expression. Reducing negative emotional expression from parents may improve depression symptoms in adolescents and promote their psychological well-being.

To analyze the public health emergencies caused by infectious diseases in schools in Sichuan Province from 2014 to 2023 and provide references for the prevention and control of infectious diseases in schools.

The relevant information on public health emergencies caused by infectious diseases in schools in Sichuan Province from 2014 to 2023 was collected through the “Reporting and Management Information System for Public Health Emergencies”. Descriptive epidemiological methods were used to analyze the event categories, temporal distribution, regional distribution, school types, etc.,as well as the relationship between the duration of the epidemic and the time interval from discovery.

From 2014 to 2023,a total of 363 public health emergencies caused by infectious diseases were reported in various schools in Sichuan Province, with 21 597 reported cases, 3 deaths, and 1 041 433 affected or exposed people. The incidence rate was 2.07%. The top five infectious diseases in terms of the number of events were varicella, influenza, mumps, other infectious diarrhea diseases, and hand - foot -mouth disease, accounting for 91.46% of the total number of events. In terms of temporal distribution, more events were reported in March and November. In terms of school types, primary schools reported the most events (195 cases), followed by junior high schools (66 cases). The morbidity rates of infectious disease emergencies varied in different regions, with the highest morbidity rate in urban schools. The ways of event discovery included daily monitoring by epidemic management personnel of grass - roots disease prevention and control centers (224 cases, 61.71%), reports from medical institutions (84 cases, 23.10%), reports from schools (23 cases, 6.34%), and notifications from superior or peer departments (14 cases, 3.56%). There was a positive correlation between the time interval from epidemic discovery and the duration of the epidemic (r=0.57, P < 0.001).

Schools are high-risk places for public health emergencies caused by infectious diseases. Targeted prevention and control measures should be taken according to the epidemic characteristics of different schools. The discovery and reporting of epidemics are extremely important for epidemic disposal. The coordination and cooperation mechanism among multiple departments such as education and health should be strengthened to improve the timeliness of monitoring and reporting of public health emergencies caused by infectious diseases in schools and gain the initiative in epidemic disposal.

To explore the relationship between substance use, problematic internet use, and their co-occurring behaviors with unintentional injuries among college students.

A cross-sectional study design was employed, with a questionnaire survey conducted among 1 700 enrolled students from two ordinary higher education institutions in Beijing. The CRAFFT scale was used to assess substance use, while the Internet Addiction Test (IAT) evaluated problematic internet use. Information on unintentional injuries and related influencing factors was also collected. Multivariate binary logistic regression was utilized to analyze the association between substance use, problematic internet use, co-occurring behaviors, and unintentional injuries, as well as to explore the influencing factors of unintentional injuries in the co-occurring behavior population.

A total of 1 626 valid questionnaires were collected. The prevalence of substance use was 35.85% (583 cases), the prevalence of problematic internet use was 52.34% (851 cases), and the co-occurrence prevalence was 20.05% (326 cases). The prevalence of unintentional injuries was 16.91% (275 cases). After adjusting for confounding factors, the multivariate logistic regression analysis indicated that compared to the control group, the odds ratio for unintentional injuries in the co-occurring behavior group was 1.57 (95%CI: 1.054-2.340), while neither substance use (aOR=1.117, 95%CI: 0.717-1.741) nor problematic internet use (aOR=1.098, 95%CI: 0.758-1.589) showed significant correlation with unintentional injuries. Among the co-occurring behavior population, suicidal ideation was identified as an independent risk factor for unintentional injuries (aOR=2.118, 95%CI: 1.184-3.787).

The simultaneous presence of substance uses and problematic internet use significantly increases the risk of unintentional injuries among college students. It is crucial to focus on high-risk populations exhibiting co-occurring behaviors, especially those with suicidal ideation, and to implement targeted preventive interventions.

To construct a stroke risk scoring tool for middle - aged and elderly Chinese people based on meta-analysis and physical examination data from primary medical institutions.

Cohort studies on risk factors for stroke onset in the Chinese population were retrieved, and predictive factors were determined through meta-analysis. Referring to the construction method of the Framingham 10-year risk scoring tool, a stroke risk scoring tool for middle-aged and elderly Chinese people was established. The Kailuan cohort and the Hongguang physical examination cohort were used as external validation populations. Indicators such as the receiver operator characteristic (ROC) curve, sensitivity, and specificity were used to evaluate the predictive efficacy of the scoring tool.

Thirty-eight studies were included in the meta-analysis, and finally 14 factors were identified as stroke predictive factors: old age, male gender, underweight, overweight, obesity, stage 1 hypertension, history of hypertension, history of diabetes, high total cholesterol, high triglycerides, low-level high-density lipoprotein, history of atrial fibrillation, family history of stroke, and current smoking. The Kailuan cohort included 137 501 people, and 2 351 cases of stroke occurred during the follow-up period. The area under the curve (AUC) was 0.74 (95%CI:0.73-0.75), the sensitivity was 0.78 (95%CI: 0.77-0.80), and the specificity was 0.59 (95%CI: 0.58-0.59). The Hongguang physical examination cohort included 7 194 subjects, and 927 people had strokes during the follow-up period. The AUC was 0.61 (95%CI: 0.59-0.63), and the sensitivity and specificity were 0.52 (95%CI: 0.50-0.56) and 0.65 (95%CI: 0.64-0.66),respectively.

The stroke risk scoring tool has good predictive efficacy in middle-aged and elderly people undergoing physical examinations. It has high feasibility for popularization and application in primary medical institutions, can help identify high-risk populations for stroke, and provide a basis for stroke prevention and intervention strategies.

To investigate the prevalence of behavioral risk factors for chronic diseases among adult residents in Quanzhou city and their clustering patterns, providing a scientific basis for developing chronic disease prevention and control policies and public health measures tailored to different regions of Quanzhou.

A descriptive analysis of the prevalence of behavioral risk factors for chronic diseases was conducted. The research data were analyzed using χ² tests via SPSS 21.0, and K-Means clustering and visualization of behavioral risk factors for chronic diseases across different regions were performed using R 4.4.0.

A total of 14 087 valid samples were collected from the social factor survey among adult residents in Quanzhou city, analyzing five primary behavioral risk factors for chronic diseases. The prevalence rates, from highest to lowest, were insufficient intake of fresh fruits and vegetables (55.86%), overweight or obesity (48.85%), current smoking (18.49%), insufficient sleep (8.03%), and excessive alcohol consumption (6.30%). The clustering patterns of behavioral risk factors for chronic diseases in the city can be categorized into three types: low fruit and vegetable intake (72.49%), health-oriented (14.82%), and excessive smoking and drinking (12.69%).

The prevalence of behavioral risk factors for chronic diseases among adult residents in Quanzhou city is relatively high and exhibits distinct clustering characteristics, predominantly characterized by low fruit and vegetable intake. The proportion of individuals with healthy lifestyles is small, indicating the need for targeted public health interventions to promote healthy lifestyles for the entire population, thereby preventing the occurrence and progression of chronic diseases.

To analyze the epidemiological characteristics and trends of drug-resistant tuberculosis (DR-TB) patients in Guizhou Province from 2016 to 2023, providing scientific evidence and support for DR-TB prevention and control strategies.

Using the “Tuberculosis Management Information System”, a subsystem of the “Chinese Disease Prevention and Control Information System”, descriptive epidemiological methods were employed to analyze the epidemiological characteristics and trends of DR-TB patients in Guizhou Province.

A total of 3 580 DR-TB patients were reported in Guizhou Province from 2016 to 2023; the male-to-female ratio was 2.15:1, with 2 976 cases (77.12%) among the Han ethnic group. The age group of 40-60 years accounted for the highest proportion, with 1 507 cases (42.09%). Compared to 2016, the proportion of patients aged 60 and above increased in 2023 (24.23% vs. 10.56%). Farmers represented the majority of the patients, totaling 1 844 cases (51.51%). The top three regions were Bijie city (676 cases, 18.88%), Guiyang city (571 cases, 15.95%), and Tongren city (531 cases, 14.83%). Compared to 2016, the proportions in Bijie and Tongren cities increased in 2023 (20.52% vs. 9.94%and 11.34% vs. 3.72%, respectively). The predominant drug resistance type was rifampicin resistance (98.41%), while the proportion of multidrug-resistant cases decreased in 2023 compared to 2016 (45.88% vs. 98.14%), and the proportion of pre-extensively drug-resistant cases increased (7.32% vs. 0%). The top three categories of registration were new patients, treatment failures, and relapses, with 1 317 cases (26.79%), 893 cases (24.94%), and 874 cases (24.41%), respectively. Compared to 2016, the proportions of new patients and relapses increased in 2023 (44.85% vs. 6.83% and 30.10% vs. 18.63%).

The prevention and control efforts for DR-TB in Guizhou Province from 2016 to 2023 have achieved certain results, but the situation remains severe. Future work should focus on continuously improving the application of molecular biological testing technologies, reducing relapses, optimizing resource allocation, and strengthening prevention and control measures for key populations and regions, ensuring timely and effective diagnosis and treatment services across all areas.

To analyze the treatment enrollment status and influencing factors for rifampicin-resistant pulmonary tuberculosis patients in Guizhou Province, providing a basis for improving prevention and control strategies.

Based on case data, drug resistance screening records, and information on drug-resistant patients in Guizhou Province from 2016 to 2023, the enrollment rate (%) of rifampicin-resistant patients was described to characterize their distribution. Chi-square tests and binary logistic regression models were employed to analyze influencing factors.

The average annual enrollment rate for rifampicin-resistant pulmonary tuberculosis patients in Guizhou Province from 2016 to 2023 was 56.00%, increasing from 35.71% in 2016 to 88.20% in 2023 (trend χ²=729.839, P<0.001). An upward trend was observed across gender, age groups (<20 years, 20-39 years, 40-59 years, and ≥60 years), and nine municipalities (P<0.05 for all). Analysis of influencing factors indicated that compared to patients who were <20 years old, local, new cases, those with reports from designated multidrug-resistant tuberculosis hospitals, and those without diagnosis delays, the following were identified as risk factors for treatment enrollment: age ≥60 years (OR=0.471, 95%CI: 0.296-0.750), migrant status (OR=0.503, 95%CI: 0.391-0.646), initial treatment positive at 2/3 months (OR=0.676, 95%CI: 0.464-0.983), reports from general tuberculosis hospitals (OR=0.080,95%CI: 0.063-0.102) and from the disease control center (OR=0.048, 95%CI: 0.040-0.057), and diagnosis delays (OR=0.551,95%CI: 0.048-0.648) (P<0.05 for all).

The treatment enrollment status of rifampicin-resistant pulmonary tuberculosis patients in Guizhou Province has gradually improved, but continued attention is necessary. It is recommended to strengthen interventions for key populations, including those aged ≥60 years, migrants, initial treatment positive at 2/3 months, patients with reports from non-multidrug-resistant designated tuberculosis hospitals, and those experiencing diagnosis delays, to further enhance treatment enrollment rates.

To explore the trends in the burden of multidrug-resistant tuberculosis (MDR-TB) in China and its associated risk factors based on the Global Burden of Disease database (GBD).

Data on the incidence, mortality, and disability-adjusted life years (DALYs) related to MDR-TB in China from 1990 to 2021 were extracted from the GBD 2021 database. The trends in disease burden and risk factors were assessed using the rate of change and annual estimated percentage change.

From 1990 to 2021, the age-standardized incidence rate (ASIR) of MDR-TB in China decreased from 3.4 504(95%UI: 0.9 415 to 9.4 455) per 100 000 to 1.4 932 (95%UI: 0.2 544 to 4.6 586) per 100 000, with an estimated annual percentage change (EAPC) of -6.6 (95%CI: -7.8 to -5.38). The age-standardized DALY rate (ASDR) fell from 40.455 (95%UI:9.522 to 116.1805) per 100 000 to 5.1077 (95%UI: 0.8967 to 15.0293) per 100 000, with an EAPC of -10.07 (95%CI: -11.24 to -8.89). The age-standardized mortality rate (ASMR) decreased from 1.176 (95%UI: 0.2691 to 3.4253) per 100 000 to 0.1469(95%UI: 0.0246 to 0.4272) per 100 000, with an EAPC of -10.03 (95%CI: -11.23 to -8.82). During the same period, the number of new cases decreased by 30.07%, the number of DALYs decreased by 77.44%, and the number of deaths decreased by 71.3%. Additionally, significant age and gender disparities were observed in the burden of MDR-TB in China. From 1990 to 2021, the declines in age-standardized incidence, DALY rates, and mortality rates were more pronounced in females than in males. Furthermore, smoking, alcohol consumption, high fasting blood glucose, and high body mass index were significant contributors to DALYs and mortality related to MDR-TB, with their contributions increasing over time.

There has been a significant downward trend in the burden of MDR-TB in China from 1990 to 2021. Attention should be given to the trends in MDR-TB burden among individuals aged 60 and above and males. Tailored prevention and control policies and measures should be developed based on the characteristics of different age groups and genders, along with the primary risk factors, to effectively reduce the overall disease burden of MDR-TB in China.

To investigate the childhood traumatic experiences, mental health symptoms, and well-being of middle school students, analyze the associations among the three, explore the mechanism by which childhood traumatic experiences and mental health symptoms affect well-being, and provide reference for improving the well-being of middle school students.

From September to November 2023, a total of 1 294 students were selected by convenience sampling and stratified sampling from two middle schools as the survey subjects. The Childhood Trauma Questionnaire, Symptom Checklist 90, and EPOCH Well-being Scale were used for the questionnaire survey. SPSS 25.0 was used for descriptive analysis, t-test, chi-square test, and Pearson correlation analysis. The plug-in process was used to explore the mediating role of mental health symptoms between childhood traumatic experiences and well-being.

There were significant differences in well-being scores among factors such as gender, school stage, household registration, parental education level, parental marital status, and“who to live with” (t=2.813, 7.11, 3.459, 9.723, 6.923, 2.515, and 20.253, P < 0.05). In this study, 90.2% of middle school students had one or more childhood traumatic experiences. The detection rates of physical abuse, physical neglect, emotional abuse, emotional neglect, and sexual abuse were 15.1%, 61%, 42.8%, 81.9%, and 10%, respectively. There were significant differences in childhood traumatic experiences among only-child status, school stage, parental education level, marital status, and family structure (t=-4.082, 5.415, 10.144, -3.032, 9.467, and -2.003, P < 0.05). All dimensions of childhood traumatic experiences and mental health symptoms were negatively correlated with well-being; except for sexual abuse, all dimensions of childhood traumatic experiences were positively correlated with mental health symptoms (r=0.301, P < 0.05). Mental health symptoms played a partial mediating role between childhood traumatic experiences and well-being, and the proportion of the mediating effect was 37.5%.

Childhood traumatic experiences and mental health symptoms negatively predict the well-being of middle school students, and mental health symptoms play a mediating role between childhood traumatic experiences and well-being. Against the background of a relatively high incidence of childhood traumatic experiences among middle school students, the well- being of middle school students can be improved by improving their mental health and reducing the impact of childhood traumatic experiences on well-being.