To explore the relationship between Internet use and cognitive function in the elderly.

Based on the data of China Family Panel Survey (CFPS) in 2020, the IADL scale and the mobile center self-rating depression scale were used to measure the disability status and depression status of the elderly. Two-column correlation analysis was used to analyze the correlation. This paper constructed a multiple mediating effect model to study the mediating path of the impact of Internet use on the cognitive function of the elderly.

After controlling for age, gender, marital status, and intergenerational support, the regression results showed that the use of the network had a significant direct impact on the cognitive function of the elderly (β=0.688, t=7.36, P<0.001), and depression and disability significantly affected the cognitive function of the elderly (β=-0.018, t=-2.696, P<0.05; β= -0.114, t=-4.83, P<0.001). The results of mediating effect analysis showed that the simple mediating effects of depression and disability between Internet use and cognitive function of the elderly were 0.044 (95% CI: 0.011 3-0.083 4) and 0.040 (95% CI:0.015 8-0.064 2), respectively, and the chain mediating effect was 0.006 (95% CI:0.000 4-0.013 2).

Depression and disability status have a mediating effect between whether or not to use the network and the cognitive function of the elderly, and depression and disability have a chain mediating effect in whether or not to use the network to affect the cognitive function of the elderly. With the popularization of the Internet, we should promote the use of the Internet in the elderly, and pay attention to the physical and mental health of the elderly, so as to protect the cognitive function of the elderly and delay or prevent the occurrence of cognitive impairment in the elderly.

To investigate the sedentary behavior of middle-aged and elderly non-diabetic population in Lanzhou and explore its correlation with insulin resistance (IR).

A cluster random sampling method was used to select residents aged 40-75 years from the survey data of the "REACTION Study" conducted between 2014 and 2016 in Lanzhou. A total of 3 609 individuals who met the inclusion criteria were included in this study. The sedentary time was divided into <3 h/d, 3-5 h/d and ≥5 h/d groups. Insulin resistance was diagnosed using the homeostasis model assessment of insulin resistance (HOMA-IR). Multivariate logistic regression analysis was performed to examine the association between sedentary behavior and IR, while subgroup analysis explored this relationship among different population subgroups.

The average daily sedentary time for study population was found to be approximately 4.00±1.92 h/d, with an overall prevalence rate of IR at 25.0%. The prevalence rates of IR for those with a sedentary time <3 h/d, 3-5 h/d and ≥5 h/d were observed as follows: 21.7%, 24.9% and 31%, respectively, with statistically significant differences (χ²=24.447, P<0.05). After adjusting for various confounding factors through logistic regression analysis, it was revealed that compared with sedentary <3 h/d, sedentary ≥5 h/d was a risk factor for the prevalence of IR (OR=1.468, 95% CI:1.168-1.845, P<0.05). Subgroup analysis indicated that only within subgroups consisting of individuals aged≥65 years old (OR=1.060,95% CI:1.005-1.118), insufficient physical activity (OR=1.071, 95% CI:1.017-1.128), hyperlipidemia (OR=1.071,95% CI:1.001-1.145), no hyperuricemia (OR=1.062, 95% CI:1.010-1.117), fatty liver (OR=1.133, 95% CI:1.038-1.237), and WC≥85 cm (OR=1.079, 95% CI:1.017-1.144), increased sedentary time was associated with increased risk of IR (all P<0.05).

Sedentary behavior is positively associated with the occurrence of IR in middle-aged and elderly non-diabetic population.However, the findings may vary across populations with distinct characteristics.

To identify the types of social networks of the elderly in China, to analyze their population distribution characteristics, and to explore the association between different social network types and health behaviors, so as to provide reference for developing personalized health intervention strategies.

Using the data from the 2018 Chinese Longitudinal Healthy Longevity Survey, latent class analysis was used to identify the types of social networks in the elderly. The Multinomial Logistic Regression Analysis and Apriori algorithm were used to analyze the association between different social network types and health-related behaviors.

A total of 6 184 elderly people was included in this study. The social network types of the elderly in China were divided into family type (45.97%), couple type (41.91%) and widowed type (12.12%).Results from the Multinomial Logistic Regression Analysis showed that the elderly who were older (OR=1.141, 95% CI: 1.132-1.149), female (OR=4.472, 95% CI: 3.846-5.201), in ethnic minority (OR=1.454, 95% CI:1.037-2.040), and had sufficient sleep (OR=1.158,95% CI: 1.010-1.328) were more likely of the family-oriented social network. The elderly who were older (OR=1.120, 95% CI: 1.109-1.131), female (OR=4.913, 95% CI: 4.007-6.026) and had sufficient sleep (OR=1.247,95% CI:1.039-1.498) were more likely of the widowed-like social network.Strong association rules of the association rule is: drinking, smoking, and exercise of the elderly social network for couples.

The couple type is more likely to have unhealthy behaviors in the elderly in China, and its social network is smaller and its social support is single. Therefore, it is necessary to enrich the social network, enhance social support, and carry out intergenerational intervention to improve the healthy behavior of the elderly.

To investigate the effect and mechanism of HBV-stimulated HTR8-S/Vneo mitochondrial antiviral signaling protein (MAVS) signaling pathway on the immune response of peripheral blood mononuclear cell (PBMC) to hepatitis B vaccine.

The HTR8-S/Vneo were co-incubated with serum of HBV-DNA positive patients. MAVS siRNA transfection was hereby applied for silencing of MAVS and MAVS plasmid transfection was applied for overexpression of MAVS in HTR8-S/Vneo. We then cocultured them with human immune cells assembly PBMC and subsequently stimulated with hepatitis B vaccine. The mRNA and protein expression levels of MAVS, NF-κB, pNF-κB, IRF3 and pIRF3 in HTR8-S/Vneo were detected by reverse transcription-quantitative real-time PCR (RT-qPCR) and flow cytometry (FCM). The levels of IL-2, IL-6, IFN-β and IL-17 were detected by enzyme-linked immunosorbent assay (ELISA), and the proportion of CD4⁺T cell were detected by flow cytometry. The data were analyzed for t test.

HBV down-regulated the mRNA expression levels of MAVS, NF-κB, IRF3 (t=6.66, P=0.003; t=14.18, P<0.001; t=3.70, P=0.021) and the protein expression levels of MAVS, NF-κB, pNF-κB (t=3.42, P=0.042; t=4.23, P=0.013; t=4.86, P=0.008), and inhibited the production of IFN-β (t=9.83, P=0.010). Silencing MAVS down-regulated the mRNA and protein expression of MAVS (t=31.20, P<0.001; t=6.53, P=0.023), inhibited the expression of IFN-β (t=5.46, P=0.032), and reduced the proportion of CD4⁺T cells in PBMC (t=9.07, P=0.001). Overexpression of MAVS induced IFN-β production (t=-34.26, P<0.001; t=-5.11, P=0.036), but the proportion of CD4⁺T cells did not show significant effect (t=-0.73, P=0.506).

HBV can inhibit the MAVS signaling pathway in HTR8-S/Vneo. Overexpression of MAVS could promote the immune response to hepatitis B vaccine in PBMC.

To explore the relationship between retinol and TTR protein, and to provide ideas for preventing Hereditary vitreous amyloidosis (HVA).

Twenty Gly83Arg mutant mice were constructed and bred (reference construction ^[2]) as the mutant group. After the irregular turbidity in the vitreous of the mutant mice eyeball was observed under slit lamp, the lens camera was used for photography, and B-ultrasound examination was conducted, and white clump-like turbidity was found in the posterior ocular region. After the turbidity in the vitreous cavity of the mice was confirmed, the mice were killed by cervical vertebrae dislocation, and DNA in liver cells was extracted and sequenced to confirm the successful construction of mutant mice. At the same time, 20 wild type C57BL/6 mice were fed as the control group, 4 mice in the mutant group and 4 mice in the control group were randomly selected from the number table, and the retinol concentration was determined by high performance liquid chromatography combined with mass spectrometry. Three mice in the mutant group and three mice in the control group were selected from a random number table. After grinding the eyeball tissues, RNA and protein were extracted. TTR gene mRNA expression, SDS-PAGE protein gel color development was detected by fluorescent quantitative PCR, and TTR gene protein expression was detected by Western Blot. The difference at P < 0.05 was statistically significant.

The mutation site was consistent with the site successfully modeled by our research group in the previous stage. Heterozygous mutation occurred at the G base at the 107th position of exon 3 (C.107G →C), and the codon of the amino acid at the 83rd position was mutated from GGC to CGC (Gly83Arg). The concentration of retinol in eye of mutant mice was lower than that of normal mice in control group, the content of retinol in liver tissue of mutant mice was higher than that in control group, and the content of retinol in blood of mutant mice was lower than that in control group (eyeball: t=6.600, P=0.022; liver: t=9.045, P<0.001; blood: t=4.343, P=0.012). Fluorescence quantitative PCR results showed that mRNA expression in eyeball tissue of mutant group was lower than that of control group (t=5.764，P=0.004). SDS-PAGE protein gel color showed that protein bands appeared in the mutant group near 11kD. Western Blot showed that TTR gene protein expression in mutant group was lower than that in control group (t=37.48，P<0.001).

The deposition of vitreous amyloid protein in the eyeball tissue of Gly83Arg mutant mice may be related to the increase of mutant TTR protein and the decrease of retinol, which has important significance for future prevention and treatment.

To explore the longitudinal bidirectional relationship between depressive symptoms and HIV infection risk behaviors among young men who have sex with men (YMSM).

A longitudinal study design was used to recruit 489 YMSM from September 2017 to January 2018 (T1), and two follow-up surveys were completed from July to September 2018 (T2) and from June to July 2019 (T3), respectively. The cross-lagged path analysis model was used to analyze the causality, time series relationship and interactive direction between depressive symptoms and HIV infection risk behaviors.

Data of 347 YMSM were analyzed using cross-lagged path analysis. The results showed that T1 depressive symptoms significantly and positively predicted T2 HIV infection risk behaviors (β=0.071, 95% CI: 0.000-0.142), and T2 depressive symptoms significantly and positively predicted T3 HIV infection risk behaviors (β=0.072, 95% CI: 0.000-0.143). However, T1 HIV infection risk behaviors did not significantly predict T2 depressive symptoms and T2 HIV infection risk behaviors did not significantly predict T3 depressive symptoms.

Depressive symptoms among YMSM significantly and positively predicted HIV infection risk behaviors, with a unidirectional causal temporal relationship. Therefore, the level of depressive symptoms should be considered as an essential factor in HIV infection risk behavior interventions for YMSM.

To investigate the relationship between 24-hour urinary sodium, urinary potassium, sodium-potassium ratio and microalbuminuria among people in elderly care institutions.

Questionnaire survey, physical examination, blood and 24 h urine samples collection were carried out in the elderly people of 16 elderly care institutions in southeastern Shanxi from September 2017 to November 2017, which were selected using the random cluster sampling method. A multivariate logistic regression model was used to analyze the association between 24 h urinary sodium excretion, urinary potassium excretion, sodium-potassium ratio and MAU.

A total of 502 subjects including 467 males (93.03%) and 33 MAU (6.57%) were included in this study (average age: 65.77±8.54 years old). The 24 h urinary sodium excretion was (193.02±87.64) mmol, urinary potassium excretion was (27.95±15.02) mmol, and the sodium-potassium ratio was (7.44±2.62), respectively. Multivariate logistic regression analysis showed that group with the highest urinary sodium excretion and the sodium-potassium ratio (Q4) compared to the lowest group (Q1) was associated with an increased risk of MAU (OR=4.026, 95% CI: 1.045-15.515; OR=4.043, 95% CI: 1.252-13.057).

The 24-hour urinary sodium and sodium-potassium ratio are positively associated with the prevalence of MAU, while 24-hour urinary potassium is not related to the prevalence of MAU.

To investigate the epidemiological characteristics and treatment outcomes of elderly patients with rifampicin-resistant tuberculosis (RR-TB) in Chongqing, and to provide evidence for the formulation of prevention and control policies for drug-resistant tuberculosis in elderly population in Chongqing. MethodsData of RR-TB patients registered in Chongqing from 2011 to 2022 were collected through the tuberculosis surveillance module of National Health Security Information Project-Disease Prevention and Control Information System. Then, RR-TB patients over 65 years were screened. Retrospectively, descriptive epidemiological methods were used to analyze the epidemiological characteristics and treatment outcomes of elderly patients with RR-TB, and to compare them with RR-TB in the whole population.

A total of 5 797 RR-TB patients were registered in Chongqing from 2011 to 2022, among which 885 were over 65 years old, accounting for 15.27%. The annual average patient registration rate of elderly RR-TB patients was 1.81/100 000, showed an increasing trend year by year.(,P<0.05). The elderly RR-TB patients were mainly male (80.90%), local population(75.03%), farmers (56.61%) and re-treatment (57.18%). The elderly RR-TB patients of local population, farmers and newly diagnosed patients increased year by year (,22.904,21.581, all P<0.05). In the number of elderly RR-TB patients, the central urban area accounted for the most (29.49%), and the city cluster of Wuling Mountain area in southeast Chongqing. There were statistical differences between different areas. In terms of region, the new area of the main city accounted for the most (29.49%), and the town of Wuling Mountain area in southeast Chongqing accounted for the lowest (12.88%), There were statistical differences between different areas (χ²=42.422, P<0.05). The treatment acceptance rate of elderly RR-TB patients registered from 2011 to 2020 was 83.52%, and the treatment success rate was only 34.59%. The main reasons for unsuccessful treatment were death (19.96%)and loss to follow(28.60%).

The epidemic situation of RR-TB in the elderly is serious.Initial drug resistance, treatment acceptance, standardized management should be paid special attention.

To intervene in the process of the information epidemic's impact on public anxiety through a mediated model with moderation, and to explore the relationship between information epidemic and anxiety and its mechanism of action, with a view to better solving the difficulties it poses, eliminating the atmosphere of public anxiety shrouded by the information epidemic under the public health emergencies, and further stifling the possibilities of the generation of social risk crises.

Using a combination of convenience sampling and snowball sampling, 1 580 valid questionnaires were surveyed nationwide, and descriptive statistical analyses, regression analyses, and mediated moderated effects analyses were conducted.

Information epidemic had a positive predictive effect on anxiety level (β=0.14, P<0.001), mental toughness partially mediated the relationship between information epidemic and anxiety level 0.30 (95% CI:0.10-0.52), with the indirect effect accounting for 15.4 % of the total effect; and education level played a moderating role in the second half of the pathway of the aforementioned mediation model (β=-0.36, P<0.001).

In the context of public health emergencies, it is recommended to improve the public's mental toughness and education level to mitigate the adverse impacts of information outbreaks, and then reduce the negative emotions of public anxiety.

To discuss the risk factors of imported food through the multi-dimensional comparative analysis of the food information published by the General Administration of Customs and to provide data reference for supervisors, and to improve the supervision of imported food.

This paper mainly collected and summarized the 48 periods of non-permitted entry food data released by the Import and Export Food Safety Bureau of the General Administration of Customs during 2019-2022 and made a comparative analysis of the sources of non-permitted entry food, food production enterprises, product types, non-permitted entry facts and other dimensions.

From 2019-2022, there were a total of 9 506 batches of food that were not allowed to enter the country, and the food that was not allowed to enter the country came from 112 countries and regions, with the highest proportion of food batches from Japan. The food came from 3 941 production enterprises, where11 countries or regions holding non-permitted entry food production enterprises, accounting for more than 50% of the total number of enterprises. According to the HS code, there were six categories and 21 chapters of non-permitted entry products, and the main non-permitted entry foods were HS03, 21, 19 and 22 products, more than 60% of the total unqualified. According to the analysis of the causes of nonconformity, there were 15 kinds, and the top 6 nonconformity causes accounted for 86% of the total nonconformity batches. There was a total of 268 types of unqualified items related to quality and safety, totaling 3 469 items.

The overall number of batches of unauthorized imported food during the period of 2019-2022 is a rising and then falling trend, and the unauthorized imported food is more concentrated in the countries of origin, manufacturers, categories of unauthorized food, reasons for failure, and quality-related unqualified items. The multi-dimensional comparative analysis provides data reference for regulators and consumers and helps the government to improve the regulation of imported food.