To establish gestational age-specific weight gain trajectories associated with birth outcomes—small for gestational age (SGA), appropriate for gestational age (AGA), and large for gestational age (LGA)—among females with different pre-pregnancy body mass index (preBMI) categories in Guangxi, China, and to compare these trajectories with the current national guidelines. The goal is to provide evidence for developing region-specific recommendations on gestational weight gain (GWG).

This study included 5 562 singleton pregnant females who delivered at the Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region between 2019 and 2021 and had complete clinical records. Gestational weight gain percentile curves were constructed for each birth weight category based on different preBMI strata and were compared with national guideline ranges. Logistic regression was used to assess associations between preBMI-specific GWG at different gestational stages and neonatal birth weight outcomes.

For AGA outcomes, the recommended total GWG ranges were 12.81-16.50 kg for underweight females, 11.87-16.17 kg for normal-weight females, 10.46-15.41 kg for overweight females, and 7.51-13.29 kg for obese females. These ranges were all slightly higher than those suggested by the national guidelines,indicating potential regional differences in gestational weight gain. Total GWG was negatively associated with the risk of SGA and positively associated with the risk of LGA, with the impact on SGA gradually diminishing as preBMI increased. For SGA,weight gain during the first and second trimesters was negatively associated with the risk of SGA in females with underweight and normal pre-pregnancy BMI, while a similar trend was observed in overweight females during the second trimester. For LGA,weight gain in the first and second trimesters was positively associated with the risk of LGA in underweight and overweight females. In normal-weight females, a similar association was observed across all trimesters, whereas in obese females, positive associations were found in the first and third trimesters.

Gestational weight gain demonstrates significant regional characteristics. Developing region-specific and preBMI-based gestational age-specific GWG guidelines may be crucial for effectively reducing the incidence of both SGA and LGA.

Based on the person-environment fit (PEF) theory, a theoretical model of worker-occupation fit related stress (MWOFS) is constructed to provide theoretical support and practical guidance for the identification and intervention of occupational stress.

Integrating theoretical analysis with empirical validation, the MWOFS model was constructed through literature comparison, inductive reasoning, and programmatic decision-making methods. A logistic regression model was used to examine the interaction effect between worker occupation fit and occupational stress on psychological, physical, and social outcomes. Furthermore, the impact of poor fit on the risk of health outcomes was calculated.

The conceptual model of the MWOFS was established based on the core elements of worker occupation fit, occupational stress, and stress related disorders. The content model provided theoretical guidance for empirical research by expanding the core elements and formulating pathway hypotheses for validation. This study included a total of 3 451 healthcare-related workers as research subjects. The empirical results showed that the interaction between misfit and occupational stress increased the risk of stress-related disorders. Compared to individuals with worker occupation fit, those with misfit exhibited elevated risks for mental responses (34.7%), physiological responses (23.1%), and chronic diseases (10.9%).

MWOFS provides a reasonable framework for integrating influencing factors of occupational stress. It also scientifically evaluates the principal health impairments in working populations arising from the interaction between workeroccupation fit and occupational stress, and proposes innovative approaches for risk stratified management of occupation related diseases and the development of targeted intervention strategies.

To analyze the independent effects and interaction of tobacco dependence level and depression on health related quality of life (HRQoL) among current smokers.

The data was from the 2021 “Psychology and Behavior Investigation of Chinese Residents” (RBICR) and a total of 1 399 cases were included in the analysis. Tobacco dependence level, depression, and HRQoL were assessed using the fagerstrom test for nicotine dependence scale, the patient health questionnaire-9 and the European five-dimensional five-level health scale. Mplus 7.0 and R software were used for analyses.

The latent class analysis supported low and high level tobacco dependence level, with mean health utility value of 1.00(0.95, 1.00) and 1.00(0.89, 1.00), respectively, and the difference was statistically significant (Z=-5.521, P<0.001). The mean health utility values of the depressed and non-depressed groups were 1.00(0.84, 1.00), 1.00(0.95, 1.00), and the difference was statistically significant (Z=-7.580, P<0.001). Tobit regression analysis found that tobacco dependence level (β=-0.030, 95% CI:-0.043~-0.018) and depression(β=-0.068, 95%CI: -0.083~-0.053) were negatively associated with health utility value. Interaction analysis found no multiplicative interactions between tobacco dependence level and depression on HRQoL (OR=1.039, 95% CI: 0.589-1.831). There was a additive interaction between tobacco dependence level and depression on HRQoL, with high levels of tobacco dependence and depression co-existing at a higher risk of unhealthiness than the two factors alone(RERI=1.411, 95% CI: 0.055-2.767), and the synergistic effect was 1.464 times greater than the sum of the effects of the two factors alone(SI=1.464, 95% CI: 0.990-2.163), and the proportion of all unhealthy smokers attributable to high levels of tobacco dependence with a synergistic effect of depression was 25.87%(AP=25.87%, 95% CI: 3.91%-47.82%). Sensitivity analysis found that AP and SI index increased.

Tobacco dependence level and depression were negatively associated with HRQoL, and there was an additive interaction on HRQoL. Reducing tobacco dependence level among current smokers, developing targeted smoking cessation interventions and preventing depressive symptoms are important for improving current smokers’ HRQoL.

To investigate whether extreme light conditions alter the expression of RNA m6A methylation regulators in brain regions during anxiety- and depression-like behaviors in mice.

A total of 48 male C57BL/6J mice were randomly divided into control, constant light, and constant dark groups (n=8 per group) for 2- or 4-week interventions.Behavioral tests assessed anxiety- and depression-like behaviors. After completion of these assessments, the medial prefrontal cortex, hypothalamus, amygdala, and ventral hippocampus were collected for qRT-PCR analysis of m6A methyltransferases and reader proteins. Differences between groups were determined using one-way ANOVA with multiple comparisons.

Compared with the control group, after 2 weeks, constant dark exposure induced significant anxiety- and depression-like behaviors (P<0.01), while constant light showed no effect. After 4 weeks, both extreme light conditions caused behavioral abnormalities (P<0.01). Compared with the control group, qRT-PCR revealed that 2-week constant dark exposure significantly altered m6A regulators: increased Mettl3 in medial prefrontal cortex (P<0.05); decreased Mettl3 and YthdF1 in hypothalamus (P<0.05); increased FTO and ALKBH5 but decreased YthdC1 in ventral hippocampus (P<0.05); and increased ALKBH5/YthdC2 but decreased YthdC1 in amygdala (P<0.05).

Constant dark exposure more readily induces anxiety- and depression-like behaviors than constant light, and extreme light conditions modify the expression profile of RNA m6A methylation regulators in emotion-related brain regions.

To understand the current situation and influencing factors of lactation mastitis in postpartum females, and to provide useful evidence for the prevention and control of lactation mastitis.

From January to October 2024, a cohort of pregnant females at 32-42 weeks of gestation was established in a tertiary hospital in Karamay city, and followed up until 42 days postpartum. Among them, 196 females who developed lactation mastitis were selected as the case group, and 196 females who did not develop lactation mastitis were matched 1:1 according to age ± 2 years as the control group. A self-designed questionnaire was used to investigate the basic information of the females. χ² test and binary logistic regression analysis were used to analyze the influencing factors.

A total of 624 pregnant females were included in this study.During the study period, 196 cases of lactation mastitis occurred, with an incidence rate of 31.41%. Binary logistic regression analysis showed that primiparity (OR=2.086, 95% CI: 1.316-3.307), nipple fissure (OR=3.593, 95% CI: 2.261-5.711),excessive milk production (OR=2.697, 95% CI: 1.393-2.261), family dysfunction (OR=2.435, 95% CI: 1.264-4.691), and co-occurrence of postpartum depression and anxiety (CAD) (OR=2.551, 95% CI: 1.369-4.755) were risk factors for lactation mastitis in postpartum females.

Hospitals and communities should fully play their supportive roles, provide personalized and systematic breastfeeding consultation and guidance for postpartum females and their family members, and emphasize the importance of family support to reduce the occurrence of lactation mastitis in postpartum females and promote breastfeeding.

To examine the relationship between postnatal passive smoking and neuropsychological development in rural 2-year-old children.

This study, based on a birth cohort study conducted in rural Yunnan Province in the previous period, followed up with assessments of passive smoking at ages 1 and 2, and a total of 293 mother-child pairs were included in the study. Passive smoking exposure was assessed through self-reports and children's urinary cotinine tests. At age 2, cognitive, language, and motor development were evaluated using the Bayley-III scales. Stata software and multivariate binary logistic regression models were used to analyze the association between between postnatal passive smoking exposure and neuropsychological development in 2-year-old children.

Passive smoking exposure rates of 293 toddlers were 45.73%at age 1 and 65.19% at age 2. Mean cognitive, language, and motor scores at age 2 were 100.73±18.60, 99.50±16.99, and 101.05±19.86, with developmental delay rates of 7.51%, 15.07%, and 14.43%, respectively. The results of the logistic regression analyses showed that passive smoking at age 1 increased the risk of language development delay (OR=2.036, 95%CI: 1.025-4.046).

Passive smoking in infancy is a risk factor for lagging language development in 2-year-old children. It is recommended to create a smoke-free growing environment for infants and toddlers to protect and promote early neuropsychological development.

To analyze the molecular characteristics and antimicrobial resistance of human-derived Salmonella strains from three cities in Henan during 2023 and 2024, providing a scientific basis for epidemiological surveillance and disease control.

Whole-genome sequencing was performed to analyze multilocus sequence typing (MLST), molecular serotyping, protein function, resistance genes, and virulence genes. Antimicrobial susceptibility was determined by the broth microdilution method.

A total of 53 human-derived Salmonella strains were collected from sentinel hospitals in Xuchang, Nanyang, and Zhoukou. The analysis revealed 16 ST types and 15 molecular serotypes, with Salmonella Enteritidis ST11 being the most prevalent (33.96%). Thirty-two virulence gene categories were identified, and all strains carried numerous genes associated with Salmonella pathogenicity islands. Fifteen classes of resistance genes were detected, with a multidrug resistance (MDR) rate of 67.92%. Minimum spanning tree clustering identified two clonal groups, and phylogenetic analysis showed a strong correlation between ST types and serotypes.

The 53 human-derived Salmonella strains harbored diverse resistance genes and highly pathogenic virulence genes, with Salmonella Enteritidis ST11 being the dominant strain.The high MDR prevalence warrants attention in disease prevention and control.

To understand parents’ attitudes towards school food education, explore the impact of school food education on parents’ dietary beliefs, and whether it could have a positive effect on dietary behavior.

A stratified cluster random sampling method was used to collect data from parents whose children are from primary school in grades 3-5 in the food education districts and counties (Pingqiao District and Xin County) and the control county (Shangcheng County) in Xinyang City. A self-administered questionnaire was used to collect information on family background, frequency of food intake, and dietary attitudes and dietary beliefs. The differences between the two groups were compared using t test，χ² test and rank sum test, and the influencing factors of parents’ dietary beliefs were analyzed using a multivariable logistic model.

The survey included 2 601 research subjects, including 1 252 in the food education group and 1 349 in the control group. The proportion of students in the food education group who regularly shared health knowledge was 51.3%, which was higher than that of the control group (33.4%) (P<0.05). In the survey of intake frequency of 7 kinds of foods, the intake of 6 kinds of foods per day in the food education group was significantly higher than that in the control group. The proportion of parents in the food education group who were aware of suggesting their children to drink milk every day was 81.1%, which was higher than the control group's proportion of 66.9% (χ²=67.37, P<0.05). The proportion of the food education group which tried to provide a variety of foods for each meal was 59.8%, which was higher than the control group's proportion of 40.1% (χ²=100.40, P<0.05).

School food education can improve parents’ beliefs of healthy dietary, thereby improving household dietary intake.Children's sharing of health knowledge could play an important role as well.

To understand the concentration characteristics of fluoride in drinking water and the health risk of population exposure in Huanggang City, analyze the epidemic status of dental fluorosis in Huanggang City, and provide scientific basis for the prevention and control of endemic fluorosis.

Drinking water samples were collected from water quality monitoring sites in 10 counties of Huanggang City from 2020 to 2024, and the prevalence of dental fluorosis in children aged 8 to 12 years was randomly sampled from 24 villages in the drinking-water fluorosis area of Huanggang City. Excel 2016 and SPSS 20.0 were used to analyze the data. The health risk assessment model recommended by the United States Environmental Protection Agency (US EPA) was used for population health risk assessment.

A total of 3 850 drinking water samples were monitored in Huanggang City from 2020 to 2024. The median fluoride concentration was 0.19 mg/L, and the concentration range was 0.03-0.86 mg/L. There were significant differences in fluoride concentration among different years, water periods, disinfection methods and regions (χ²=60.695, Z=-8.908, χ²= 19.585, χ²=3 754.425; all P<0.001). Water fluorine was strongly correlated with pH, total dissolved solids and chloride (r=0.619, 0.520, 0.516, respectively; all P<0.001). The median and maximum hazard quotient (HQ) values for non-carcinogenic health risk from fluoride exposure through drinking water intake were less than 1, and the difference of non-carcinogenic health risk values among different regions was statistically significant (H=77.626, P=0.027). A total of 5 667 children from infected villages in Huanggang City were monitored from 2020 to 2024. The average annual detection rate of dental fluorosis was 0.49%, and the detection rate in five years ranged from 0.11%to 0.74%, with statistically significant differences among different regions (χ²=123.584, P<0.001).

From 2020 to 2024, the qualified rate of fluoride content in drinking water in Huanggang City was 100%, and the detection rate of dental fluorosis was less than 30%, which met the national standard of elimination of endemic fluorosis, but there are obvious differences between regions. Relevant departments need to focus on the health risk of fluoride exposure and the epidemic trend of dental fluorosis in Hong’an and Wuxue areas. We will take targeted measures to prevent and control endemic fluorosis.

This study aims to explore the impact of Fitness Qigong on depression in middle-aged and elderly, as well as the chain mediating role of mindfulness and sense of meaning in life.

The study utilized the International Physical Activity Questionnaire, the Meaning in Life Questionnaire (MLQ), the Mindful Attention Awareness Scale (MAAS), and the Patient Health Questionnaire-9 (PHQ-9). Taking middle-aged and elderly people in Guangxi Autonomous Region as the research subjects. A total of 1 000 questionnaires were distributed, with 956 valid responses returned, resulting in a 95.6%effective response rate. Descriptive statistics, correlation analysis, and regression analysis were conducted using SPSS 27.0. The Bootstrap method in the PROCESS V4.1 plug-in was used to test the mediating effect and verify the chain mediating role of mindfulness and meaning in life.

The depression detection rate among middle-aged and elderly people in Guangxi was 17.3%. Depression scores showed significant differences in terms of age, education level, marital status, and exercise intensity of Qigong. Fitness Qigong exercise has a significant direct negative effect on depression levels among middle-aged and elderly individuals (β=-0.30, P < 0.001). Mindfulness and meaning in life exhibited significant individual mediating effects in the relationship between Fitness Qigong exercise and depression, with effect values of -0.059 (95% CI: -0.087~-0.036) and-0.022(95% CI: -0.041~-0.005), accounting for 15.42% and 5.63% of the total effect，respectively. Additionally, the chain mediating effect was also significant, with an effect value of -0.008(95% CI: -0.017~-0.002), accounting for 2.16% of the total effect.

Fitness Qigong not only directly reduces depression levels in the elderly but also affects depression levels through the chain mediating effects of mindfulness and meaning in life.