To analyze the independent effects and interaction of tobacco dependence level and depression on health related quality of life (HRQoL) among current smokers.

The data was from the 2021 “Psychology and Behavior Investigation of Chinese Residents” (RBICR) and a total of 1 399 cases were included in the analysis. Tobacco dependence level, depression, and HRQoL were assessed using the fagerstrom test for nicotine dependence scale, the patient health questionnaire-9 and the European five-dimensional five-level health scale. Mplus 7.0 and R software were used for analyses.

The latent class analysis supported low and high level tobacco dependence level, with mean health utility value of 1.00(0.95, 1.00) and 1.00(0.89, 1.00), respectively, and the difference was statistically significant (Z=-5.521, P<0.001). The mean health utility values of the depressed and non-depressed groups were 1.00(0.84, 1.00), 1.00(0.95, 1.00), and the difference was statistically significant (Z=-7.580, P<0.001). Tobit regression analysis found that tobacco dependence level (β=-0.030, 95% CI:-0.043~-0.018) and depression(β=-0.068, 95%CI: -0.083~-0.053) were negatively associated with health utility value. Interaction analysis found no multiplicative interactions between tobacco dependence level and depression on HRQoL (OR=1.039, 95% CI: 0.589-1.831). There was a additive interaction between tobacco dependence level and depression on HRQoL, with high levels of tobacco dependence and depression co-existing at a higher risk of unhealthiness than the two factors alone(RERI=1.411, 95% CI: 0.055-2.767), and the synergistic effect was 1.464 times greater than the sum of the effects of the two factors alone(SI=1.464, 95% CI: 0.990-2.163), and the proportion of all unhealthy smokers attributable to high levels of tobacco dependence with a synergistic effect of depression was 25.87%(AP=25.87%, 95% CI: 3.91%-47.82%). Sensitivity analysis found that AP and SI index increased.

Tobacco dependence level and depression were negatively associated with HRQoL, and there was an additive interaction on HRQoL. Reducing tobacco dependence level among current smokers, developing targeted smoking cessation interventions and preventing depressive symptoms are important for improving current smokers’ HRQoL.

To explore the cognition of medical staff within the tightly-knit county-level medical consortium in Xinyi County, Jiangsu Province regarding the reform of medical insurance payment methods and the influencing factors, and to provide references for deepening the reform of medical insurance payment methods within the medical consortium.

By using stratified cluster sampling method, a questionnaire survey was conducted among 1 415 medical staff within the medical consortium of Xinyi City from October 2021 to January 2022. The random forest model was employed to screen variables and multivariate logistic regression analysis was conducted to analyze the influencing factors.

Medical staff had a relatively high level of understanding of the medical insurance payment methods of disease-based payment (87.1%) and service-item-based payment (74.3%); they had a relatively high level of recognition of the medical insurance payment methods of disease-based payment (75.1%) and bundled payment based on medical consortium (51.7%). 91.7% of the medical staff were relatively familiar with the reform. The working institutions were township health centers and community health service centers (OR=2.300, 95% CI: 1.205-4.391), and medical staff with senior professional titles or above (OR=4.701, 95% CI: 1.610-13.727) had a relatively high level of understanding of the reform. 81.5% of the medical staff expressed their recognition of the reform. Doctors (OR=2.032, 95% CI: 1.459-2.828), nurses (OR=1.638, 95% CI: 1.073-2.501), medical staff with a master's degree or above (OR=2.399, 95% CI: 1.050-5.480), and medical staff with shorter working years (OR_11-15years=0.546, 95% CI: 0.327-0.911; OR_16-20years=0.516, 95% CI: 0.285-0.932; OR_≥21years=0.443, 95% CI:0.272-0.722) had a relatively high level of recognition of the reform.

The level of awareness and acceptance of new payment methods such as bundled payment for total medical expenses by medical staff within the tightly-knit county-level medical consortium still needs to be improved. It is suggested that measures such as stratified training and the introduction of talent incentive policies for grassroots medical institutions be adopted to enhance the awareness and recognition of medical staff.

To analyze the molecular characteristics and antimicrobial resistance of human-derived Salmonella strains from three cities in Henan during 2023 and 2024, providing a scientific basis for epidemiological surveillance and disease control.

Whole-genome sequencing was performed to analyze multilocus sequence typing (MLST), molecular serotyping, protein function, resistance genes, and virulence genes. Antimicrobial susceptibility was determined by the broth microdilution method.

A total of 53 human-derived Salmonella strains were collected from sentinel hospitals in Xuchang, Nanyang, and Zhoukou. The analysis revealed 16 ST types and 15 molecular serotypes, with Salmonella Enteritidis ST11 being the most prevalent (33.96%). Thirty-two virulence gene categories were identified, and all strains carried numerous genes associated with Salmonella pathogenicity islands. Fifteen classes of resistance genes were detected, with a multidrug resistance (MDR) rate of 67.92%. Minimum spanning tree clustering identified two clonal groups, and phylogenetic analysis showed a strong correlation between ST types and serotypes.

The 53 human-derived Salmonella strains harbored diverse resistance genes and highly pathogenic virulence genes, with Salmonella Enteritidis ST11 being the dominant strain.The high MDR prevalence warrants attention in disease prevention and control.

Analyze the disease burden of inflammatory bowel disease (IBD) in females in China and globally to provide reference for the prevention and control of IBD in females.

The data on IBD in females in China and globally were extracted from the most recent update of the Global Burden of Disease (GBD) study available as of 2021. Age-standardized incidence rates (ASIR), age-standardized prevalence rates (ASPR), age-standardized mortality rates (ASMR), and age-standardized DALYs rates (ASDR) were calculated. Joinpoint analysis was used to estimate the annual percentage change (APC) and the average annual percentage change (AAPC).

From 1990 to 2021, the ASIR and ASPR of IBD among Chinese females showed upward trends, with AAPC of 2.1% and 1.55%, respectively. Meanwhile, ASMR and ASDR exhibited downward trends, with AAPC values of -3.45% and -3.67%, respectively. Globally, the ASIR of IBD in females increased at an AAPC of 0.17%, while ASPR, ASMR, and ASDR all demonstrated downward trends, with AAPC of -0.25%, -0.53%, and-0.58%, respectively.

Chinese females exhibit significantly higher growth rates in IBD incidence and prevalence compared to global female averages, while demonstrating more pronounced declines in mortality and DALYs burden than global female averages. Although current disease burden remains lower than the global average, the rapid escalation highlights substantial health threats.

To investigate the effect of proteasome activator PSME1 on proteasome function and antioxidant stress capacity in endothelial cells.

An adenoviral vector (Ad-PSME1) was used to transduce the target gene into human umbilical vein endothelial cells (HUVEC) to establish PSME1-overexpressing endothelial cells. Western blot was used to detect the expression levels of proteasome-related proteins; dot blot was employed to assess protein carbonylation levels; co-immunoprecipitation(Co-IP) was performed to examine the interaction between PSME1 and PSME2; fluorogenic substrate assays were used to determine proteasome activity;cycloheximide(CHX) chase experiments were conducted to evaluate protein degradation rates; pulse-chase assays were applied to measure the degradation rate of GFPu; and DNPH derivatization was used to detect protein carbonylation levels.

Overexpression of PSME1 in HUVECs increased the expression and prolonged the half-life of PSME2, enhanced chymotrypsin-like (β5) proteasome activity and ATP-dependent proteolytic function, and significantly shortened the half-life of misfolded protein GFPu(P<0.05). No significant effects were observed on the stability of endogenous UPS substrates(AKT, GATA4, and PTEN) or on the abundance of the 19S and 20S proteasome complexes (RPN2, RPT6, and PSMB5) (P>0.05). Additionally, PSME1 overexpression significantly reduced hydrogen peroxide-induced protein carbonylation levels and enhanced the antioxidant stress capacity of endothelial cells(P<0.05).

Overexpression of PSME1 stabilizes PSME2, enhances the function of the 11S regulatory particle, and improves the proteasome's ability to degrade misfolded and oxidatively damaged proteins, thereby protecting endothelial cells.

To investigate the association between the ratio of triglycerides (TG) to high-density lipoprotein cholesterol (HDL-C) and the incidence of hypertension in middle-aged and elderly Chinese adults, and to provide a reference for the development of hypertension intervention strategies.

This study included 6 231 participants from the baseline and 2015 follow-up data of the China Health and Retirement Longitudinal Study (CHARLS). Logistic regression models were used to analyze the relationship between the TG/HDL-C ratio and the prevalence of hypertension in middle-aged and elderly populations. After excluding individuals diagnosed with hypertension before 2015, the cumulative TG/HDL-C ratio was calculated, and logistic regression models were employed to analyze the relationship between the cumulative TG/HDL-C ratio and the incidence of hypertension. Additionally, restricted cubic spline (RCS) models were used to fit the dose-response relationship between the cumulative TG/HDL-C ratio and the risk of hypertension in middle-aged and elderly populations.

From 2011 to 2015, 2 438 individuals (39.13%) were diagnosed with hypertension, and in 2015, 625 individuals (16.48%) developed hypertension. Logistic regression analysis revealed that an elevated TG/HDL-C ratio was significantly associated with an increased risk of hypertension (OR=1.02, 95% CI: 1.02-1.03). The highest quartile of the TG/HDL-C ratio was identified as a risk factor for hypertension (OR=1.34，95% CI：1.02-1.74). Furthermore, a linear association was observed between the cumulative TG/HDL-C ratio and the incidence of hypertension (P-nonlinear=0.232).

An elevated TG/HDL-C ratio is a risk factor for hypertension in middle-aged and elderly Chinese adults.

This study aims to explore the impact of Fitness Qigong on depression in middle-aged and elderly, as well as the chain mediating role of mindfulness and sense of meaning in life.

The study utilized the International Physical Activity Questionnaire, the Meaning in Life Questionnaire (MLQ), the Mindful Attention Awareness Scale (MAAS), and the Patient Health Questionnaire-9 (PHQ-9). Taking middle-aged and elderly people in Guangxi Autonomous Region as the research subjects. A total of 1 000 questionnaires were distributed, with 956 valid responses returned, resulting in a 95.6%effective response rate. Descriptive statistics, correlation analysis, and regression analysis were conducted using SPSS 27.0. The Bootstrap method in the PROCESS V4.1 plug-in was used to test the mediating effect and verify the chain mediating role of mindfulness and meaning in life.

The depression detection rate among middle-aged and elderly people in Guangxi was 17.3%. Depression scores showed significant differences in terms of age, education level, marital status, and exercise intensity of Qigong. Fitness Qigong exercise has a significant direct negative effect on depression levels among middle-aged and elderly individuals (β=-0.30, P < 0.001). Mindfulness and meaning in life exhibited significant individual mediating effects in the relationship between Fitness Qigong exercise and depression, with effect values of -0.059 (95% CI: -0.087~-0.036) and-0.022(95% CI: -0.041~-0.005), accounting for 15.42% and 5.63% of the total effect，respectively. Additionally, the chain mediating effect was also significant, with an effect value of -0.008(95% CI: -0.017~-0.002), accounting for 2.16% of the total effect.

Fitness Qigong not only directly reduces depression levels in the elderly but also affects depression levels through the chain mediating effects of mindfulness and meaning in life.

To investigate the longitudinal association between the metabolic score for insulin resistance (METS-IR) and the risk of cardiovascular disease (CVD) and its subtypes (heart disease and stroke) in middle-aged and older adults.

This study used data from 4 567 participants in the China Health and Retirement Longitudinal Study (CHARLS) cohort. METS-IR levels measured in 2011 and 2015 were standardized, and K-means cluster analysis was used to classify participants into four categories (Class 1-4). Cumulative METS-IR exposure levels were calculated. Multivariable Cox regression models, adjusted for potential confounders, were used to evaluate the association between METS-IR and incident CVD. Restricted cubic spline analysis assessed the dose-response relationship between cumulative METS-IR exposure and CVD risk. Subgroup analyses explored heterogeneity by gender and age. The predictive performance of METS-IR for CVD was evaluated using time-dependent receiver operating characteristic (ROC) curves.

Over a median follow-up of 3 years, 531 CVD events occurred among 4 567 participants. Cox regression analysis showed that compared to Class 1 (lowest persistent METS-IR levels), Class 4(highest persistent METS-IR levels) was associated with a 77% increased risk of incident CVD (HR=1.77, 95%CI: 1.24-2.52), including a 67% increased risk of heart disease (HR=1.67, 95%CI: 1.10-2.54) and a significant 117% increased risk of stroke (HR=2.17,95%CI: 1.31-3.59). A significant linear dose-response relationship was observed between cumulative METS-IR exposure and CVD risk. ROC curve analysis indicated that cumulative METS-IR had the best predictive performance for CVD, with an area under the curve (AUC) of 0.613.

Persistently elevated longitudinal levels of METS-IR are associated with an increased risk of incident CVD. Dynamic monitoring of METS-IR levels is important for the prevention and treatment of CVD.

To investigate whether extreme light conditions alter the expression of RNA m6A methylation regulators in brain regions during anxiety- and depression-like behaviors in mice.

A total of 48 male C57BL/6J mice were randomly divided into control, constant light, and constant dark groups (n=8 per group) for 2- or 4-week interventions.Behavioral tests assessed anxiety- and depression-like behaviors. After completion of these assessments, the medial prefrontal cortex, hypothalamus, amygdala, and ventral hippocampus were collected for qRT-PCR analysis of m6A methyltransferases and reader proteins. Differences between groups were determined using one-way ANOVA with multiple comparisons.

Compared with the control group, after 2 weeks, constant dark exposure induced significant anxiety- and depression-like behaviors (P<0.01), while constant light showed no effect. After 4 weeks, both extreme light conditions caused behavioral abnormalities (P<0.01). Compared with the control group, qRT-PCR revealed that 2-week constant dark exposure significantly altered m6A regulators: increased Mettl3 in medial prefrontal cortex (P<0.05); decreased Mettl3 and YthdF1 in hypothalamus (P<0.05); increased FTO and ALKBH5 but decreased YthdC1 in ventral hippocampus (P<0.05); and increased ALKBH5/YthdC2 but decreased YthdC1 in amygdala (P<0.05).

Constant dark exposure more readily induces anxiety- and depression-like behaviors than constant light, and extreme light conditions modify the expression profile of RNA m6A methylation regulators in emotion-related brain regions.

Based on the person-environment fit (PEF) theory, a theoretical model of worker-occupation fit related stress (MWOFS) is constructed to provide theoretical support and practical guidance for the identification and intervention of occupational stress.

Integrating theoretical analysis with empirical validation, the MWOFS model was constructed through literature comparison, inductive reasoning, and programmatic decision-making methods. A logistic regression model was used to examine the interaction effect between worker occupation fit and occupational stress on psychological, physical, and social outcomes. Furthermore, the impact of poor fit on the risk of health outcomes was calculated.

The conceptual model of the MWOFS was established based on the core elements of worker occupation fit, occupational stress, and stress related disorders. The content model provided theoretical guidance for empirical research by expanding the core elements and formulating pathway hypotheses for validation. This study included a total of 3 451 healthcare-related workers as research subjects. The empirical results showed that the interaction between misfit and occupational stress increased the risk of stress-related disorders. Compared to individuals with worker occupation fit, those with misfit exhibited elevated risks for mental responses (34.7%), physiological responses (23.1%), and chronic diseases (10.9%).

MWOFS provides a reasonable framework for integrating influencing factors of occupational stress. It also scientifically evaluates the principal health impairments in working populations arising from the interaction between workeroccupation fit and occupational stress, and proposes innovative approaches for risk stratified management of occupation related diseases and the development of targeted intervention strategies.