To understand the current situation of non-suicidal self-injury (NSSI) behavior among middle school students in Taiyuan, and to explore the relationship between socio-ecological risk factors and NSSI behavior of middle school students, so as to provide evidence for targeted intervention strategies.

A total of 2 635 middle and high school students in Taiyuan were selected by stratified random cluster sampling. The general demographic characteristics, the risk level of social ecology, and the occurrence of NSSI behavior in the past year were investigated. Binary Logistic regression was used to analyze the relationship between NSSI behavior and socio-ecological risk factors.

The reporting rate of NSSI behavior of middle school students was 13.5%. There were significant differences in NSSI behavior report rate among middle school students with different genders (χ²=11.12), the number of close friends (χ²=30.52), and different learning burden (χ²=37.51). After adjusting gender, the number of close friends, and learning burden factors (all P<0.01), the Logistic regression model took the low risk level of social and ecological risk factors as the control group, high risk personal factor (OR=2.93, 95%CI: 1.87-4.59), high risk family factor (OR=3.10, 95%CI: 2.04-4.71), moderate risk family factor (OR=2.08, 95%CI: 1.40-3.08), high risk school factors (OR=2.36, 95%CI:1.48-3.76), and high risk cultural factors (OR=1.44, 95%CI: 1.01-2.06) were positively correlated with NSSI behavior of middle school students.

When middle school students are exposed to high risk levels of individuals, families, schools and cultures in the social ecosystem, the risk of NSSI behavior will increase. While carrying on the intervention of personal, family and school factors, we should also pay attention to the influence of cultural factors on the NSSI of middle school students.

To investigate the relationship between frailty and social activity of the elderly in China, and to study the influencing factors of frailty in the elderly, so as to provide scientific basis for the healthy life of the elderly.

Based on 43 health related variables in China Health and Retirement Longitudinal Study (CHARLS) 2018 database, the frailty index (FI) was constructed to evaluate the frailty of the elderly. Binary logistic regression was used to explore the effect of social activity on the frailty of the elderly.

A total of 1 519 elderly people aged 60 and above were included in the CHARLS 2018 database, including 401 frail elderly, with a frailty rate of 26.4%. The participation rate of the elderly in social activities was 48.5%,and there were 439 elderly people with high level of social activity, accounting for 28.9% of the total. After adjusting for confounding factors, the risk of debilitation in the elderly with high level of social activity was 41.9%, lower than that in those with medium and low level of social activity (OR=0.581, 95%CI: 0.439-0.77).

The degree of social activity affects the frailty of the elderly, and the social activity of the elderly in China is generally low. The elderly should be encouraged to participate in social activities, and attention should be paid to the older and female elderly.

To analyze the characteristics of envelope protein (envelope, E) gene in dengue fever cases in Hubei Province from 2014 to 2023 and to trace the biological origin.

Serum samples of suspected dengue fever cases were collected, and nucleic acid detection and serum typing were carried out. Nucleic acid positive samples were amplified by RT-PCR and sequenced, and the gene sequences were obtained and analyzed by MEGA11.0 software.

A total of 78 dengue nucleic acid positive cases were detected in Hubei Province from 2014 to 2023, and the E gene sequence of dengue virus (DENV) was successfully obtained in 44 samples, including 39 cases of serum type Ⅰ (DENV-Ⅰ) and 5 cases of serum typeⅡ (DENV-Ⅱ). Evolutionary analysis showed that 37 cases of DENV-Ⅰ were of gene type Ⅰ (G Ⅰ), distributed in three different branches, mainly close to the epidemic strains of Southeast Asian countries such as Guangzhou, Singapore, Indonesia, Thailand, and Myanmar, 2 cases were of gene type Ⅴ (GⅤ) were close to the epidemic strains of Guangzhou and India, and 5 cases of DENV-Ⅱ were of mixed type (Cosmopolitan type).

There was multitype dengue virus infection in Hubei Province from 2014 to 2023. The main epidemic strain was GⅠ subtype of DENV-Ⅰ, which was closely related to Guangzhou, China, and Southeast Asian countries. It is suggested that Hubei Province strengthen the prevention and control of cross-provincial and cross-border transmission of dengue fever and guard against the occurrence of severe dengue and death cases.

To analyze the spatial distribution characteristics, efficiency, and influencing factors of pension institutions in Dongguan, so as to provide reference for optimizing the resource allocation of pension institutions in Dongguan.

The resource allocation data of pension institutions in Dongguan were obtained by questionnaire survey, and the spatial distribution characteristics and efficiency of pension institutions were analyzed and evaluated based on GIS and three-stage DEA model.

There was a good spatial coupling between the resources of pension institutions in Dongguan and the elderly population over 60 years old; the lowest occupancy rate was 12.50%, the highest was 100.005%, and the phenomenon of “difficulty in finding a bed” and “half of the beds were vacant” coexisted. In the third stage, the average comprehensive efficiency decreased from 0.679 to 0.635, and environmental factors had a great influence on the efficiency of resource allocation in Dongguan. There were great differences in the efficiency of pension institutions in different areas. The comprehensive efficiency of urban areas was 0.784, which exceeded the average level of the whole city (0.635). The comprehensive efficiency of the eastern industrial park was only 0.439, which did not reach the average level of the whole city. The per capita regional GDP and the level of aging had a negative impact on efficiency.

It is suggested to rationally allocate the resources of pension institutions, optimize regional development planning, promote the upgrading of institutional pension services, improve the efficiency of resource allocation, and provide improved reference scheme for improving the service level of pension institutions in Dongguan.

To analyze the multiple mediating effects of social support and sense of meaning of life on the quality of death in elderly terminally ill patients.

By using convenience sampling method, 276 elderly patients and their attending nurses were investigated with general data questionnaire, perceived social support scale, life significance source scale for the elderly, family care index scale, and ICU nurse version QODD-14 scale. Pearson was used to analyze the correlation, and Amos software was used to establish structural equation model (SEM) and mediate effect test.

(1) The scores of quality of death, family function, social support, and sense of life were 62.52±8.67, 7.54±1.14, 52.53±7.64, and 107.54±13.58, respectively. (2) The quality of death of elderly deathbed patients was positively correlated with family function, social support, and sense of life meaning (r = 0.762, 0.886, and 0.795, P < 0.05). Family function was positively correlated with social support and sense of life meaning (r = 0.528, 0.499, P < 0.05). There was a positive correlation between social support and sense of life meaning (r = 0.665, P < 0.05). (3) Specific intermediary effect of social support accounted for 20.22%, specific intermediary effect of sense of life meaning accounted for 17.06%, and multiple intermediary effect of social support-sense of life meaning accounted for 6.54%.

Family function can indirectly affect the quality of death by regulating social support and sense of life meaning.

To analyze the relationship between the proportion of omega-6/omega-3 and mortality in patients with cardiovascular disease (CVD), so as to provide reference for reducing the risk of death in patients with CVD.

The data were collected from the UK perspective cohort (UK Biobank) database. The exposure variable was the proportion of plasma omega6/omega-3, and the outcomes were all- cause deaths, CVD deaths, ischemic heart disease (IHD) deaths, and stroke deaths. Multivariate Cox proportional hazard regression model was used to analyze the relationship between omega-6/omega-3 ratio and death risk of CVD patients. Hazard ratio (HR) and 95% confidence interval (CI) were calculated. Gender, age, and other risk factors were stratified and their interaction with omega-6/omega-3 ratio was analyzed. Restricted cubic spline (RCS) was used to describe the dose-response relationship. Sensitivity analysis was used to test the robustness of the model.

A total of 35 096 participants were enrolled. Multivariate Cox proportional hazard regression model showed that all-cause deaths, CVD deaths, and ischemic heart disease deaths risk increased in CVD patients with the increase of the proportion of omega-6/omega-3. For each unit increase in omega-6/omega-3 ratio, the risk of all-cause, CVD, and IHD deaths increased by 2.1% (HR=1.021, 95%CI: 1.016-0.025), 1.9% (HR=1.019, 95%CI: 1.010-1.028), and 2.3% (HR=1.023, 95%CI:1.013-1.034), respectively. The proportion of omega-6/omega-3 was grouped according to quartile method and included in the model as a classified variable. Compared with Q1, the HR of all-cause, CVD, and IHD deaths of Q4 was 1.394 (95%CI: 1.267-1.534), 1.292 (95%CI: 1.085-1.540), and 1.528 (95%CI: 1.200-1.945), respectively, and no correlation was found between Q4 and stroke death risk (HR=0.987, 95%CI: 0.660-1.477). The results of RCS showed that the proportion of omega-6/omega3 was positively correlated with the risk of death.

The proportion of omega-6/omega-3 is a risk factor for death in patients with CVD.

To describe the characteristics of commercial sexual behavior of HIV-infected men who have sex with men (MSM) and analyze its influencing factors.

An anonymous questionnaire survey was conducted through the recruitment of HIV-infected MSM by two social organizations in Chongqing. The χ² test was used to compare the commercial sexual behavior of HIV infected with MSM with different social demographic characteristics, mental health status, and sexual behavioral characteristics. Multivariate Logistic regression model was used to analyze the influencing factors of commercial behavior.

A total of 1 501 cases of HIV infection with MSM were investigated in this study. In total 12.50% of the respondents reported same-sex commercial sex in the past six months, of which 28.34% reported unprotected commercial sex behavior. The results of multivariate Logistic regression showed HIV-infected MSM with marital status as marriage/cohabitation (OR=2.345, 95%CI: 1.442-3.814), working status as part-time and others (OR=6.522, 95%CI: 3.575-11.900), monthly income exceeding 5 000 yuan (OR=7.890, 95%CI: 4.254-14.634), age of first sexual intercourse less than 18 years old (OR=1.818,95%CI: 1.014-3.260), using same-sex dating software (using one: OR=2.984, 95%CI: 1.477-6.030; using two: OR=3.683,95%CI: 1.650-8.221; using 3 and above: OR=21.777, 95%CI: 8.349-56.804), drinking (OR=2.528, 95%CI: 1.568-4.074), and using Rush (OR=3.114, 95%CI: 1.988-4.877) were more likely to have commercial sexual behavior (P < 0.05).

The commercial sexual behavior of HIV infection with MSM is common in Chongqing, and the proportion of commercial sexual behavior without condom is high. Comprehensive measures should be taken to intervene the drinking, Rush use, and commercial sexual behavior of this population in order to reduce the further spread of HIV/STIs.

To study the relationship between social participation and depression trajectory and to explore the mediating effect of quality of life.

Based on the data of the four periods of China Health and Retirement Longitudinal Study (CHARLS) from 2011 to 2018, the heterogeneous trajectory of depressive symptoms in the elderly was identified by group-based trajectory model, the relationship between social participation and depression trajectory was studied by multi-class logistic regression, and the mediating effect of quality of life on social participation and depression trajectory of the elderly was analyzed by Karlson-Holm-Breen （KHB） method.

A total of 4 931 elderly people aged between 60 and 105 with at least two periods of depression were included, and four depression trajectory groups were identified: long-term non-depression group (61.69%), increased depression group (16.69%), depression remission group (13.83%), and long-term high depression group (7.79%). Compared with the subjects in the long-term non-depression group, the more social participation, the less likely the depression trajectory was attributed to the depression remission group, the increased depression group, and the long-term high depression group (RRR=0.899, 95%CI: 0.812-0.994; RRR=0.885, 95%CI: 0.786-0.995; RRR=0.747, 95%CI: 0.630-0.885). In addition, quality of life played a significant mediating role in the relationship between the two, and the mediating effect of self-rated health accounted for the largest contribution of 30.33% in the elevated depression group. The mediating effect of physical pain accounted for the largest contribution of 27.94% and 29.09, respectively in the depression remission group and the long-term high depression group. Similar results were obtained in the robustness test.

There is heterogeneity in the depression trajectory of the elderly, social participation has a significant impact on the attribution of depression trajectory, and quality of life is an important factor of social participation affecting depression trajectory.

To understand the distribution, serotype, and drug resistance of Salmonella in domestic sewage in Jining city, and to analyze the whole genome sequencing so as to provide scientific basis for the prevention and control of Salmonella.

From February to March in 2023, two samples of sewage from the intake of 3 urban sewage plants with 500 ml each were collected continuously for 7 weeks. A total of 42 sewage samples were collected for culture, isolation, and identification of Salmonella. The isolated Salmonella was tested for serum agglutination test and fluorescent quantitative PCR serotyping. In total 41 strains of Salmonella were tested for minimum inhibitory concentration (MIC) of 17 drugs in 9 classes by microbroth dilution method, followed by genome sequencing and phylogenetic analysis.

The isolation rate of Salmonella from domestic sewage in Jining city was high. Among 24 positive samples, 41 strains of Salmonella were isolated, belonging to 9 serotypes, mainly S. Agona and S. Kentucky. Liying and Jidong sewage treatment plants had their own unique serotypes. Only one strain of S. Give was not resistant, and the other 40 strains of Salmonella were multi-drug resistant. The resistance rates to sulfamethoxazole, tetracycline, ampicillin, and streptomycin were more than 90%, and the resistance rate to ampicillin/sulbactam was more than 80%. There were 19 kinds of drug resistance spectra, of which SXT-TET-STR-AMP-AMS ranked the first place. The resistance spectra of different serotypes of Salmonella were different. S. Kentucky, S. Gold Coast, and S. Infant were multi-drug resistant seriously. The resistance spectrum of the same serotype was also inconsistent in different sewage plants, and the resistance was the most serious in Liying sewage plant. The CARD database was used to predict drug resistance genes, mainly predicting 18 drug resistance genes in 8 types of drug resistance families, and the drug resistance phenotypes and drug resistance genes were almost all consistent. The ST types of different serotypes of Salmonella were different, and ST13 S. Agona was dominant. The phylogenetic tree was divided into 6 clusters, and strains in different sewage plants were in different branches, indicating that their genomes were different and had different sources.

There are various serotypes of salmonella in domestic sewage in Jining city, suggesting that they might have exist and spread in the population. Almost all strains are multi-drug resistant bacteria, carrying resistance genes extensively. Some types of strains live persistently and distribute across regions, leading to the potential risk to population. It is necessary to carry out continuous monitor, and scientific prevention and control measures need to be formulated to control the epidemic of salmonella and the aggravation of drug resistance.