Latest ArticlesFoodborne pathogenic bacteria are one of the major factors leading to food safety incidents.Traditional detection methods have limitations such as time consumption, low sensitivity and complicated operation.In recent years, with the rapid development of molecular biology, immunology, biosensor technology and point-of-care testing (POCT) technology, a variety of rapid detection technologies for foodborne pathogenic bacteria have emerged. This paper systematically reviewed the latest research progress in the field of rapid detection of pathogenic bacteria in food, focused on the principles and characteristiced of molecular biology technologies (mass spectrometry, polymerase chain reaction and its derivative technologies, isothermal amplification, high-throughput sequencing) and immunological technologies (enzyme-linked immunosorbent assay, immunochromatography,immunosensors). It also summarized the application status of POCT technology formed by the interdisciplinary integration of molecular biology, nanotechnology, immunology and information technology. Meanwhile, it analyzed the challenges faced by various technologies, and prospected the future development trends, aimed to provide technical references for the monitoring of pathogenic bacteria in food.
To investigate the optimal processing parameters for Ipomoea batatas-Morus alba flavored yogurt, analyze its quality, and develop a yogurt with a unique flavor and excellent quality.
First,the process conditions—including the ratio of Ipomoea batatas powder to Morus alba juice, sugar addition, and fermentation time—were optimized through single-factor and response surface experiments, using viscosity,water-holding capacity, whey separation rate, acidity and sensory evaluation as indicators Subsequently, under the optimized process conditions, the quality of the yogurt was analyzed based on indicators such as protein, fat, non-fat milk solids, acidity, lactic acid bacteria count and mold count.
The optimal process parameters for Ipomoea batatas-Morus alba yogurt were determined as follows: A Ipomoea batatas powder to Morus alba juice ratio (m:m) of 3:5, a sucrose addition of 6% and a fermentation time of 5.0 h. Under these conditions, the sensory score of the resulting yogurt was 87.04 points, which was very close to the theoretically predicted value of 87.32 points. Analysis of the quality indicators of this yogurt revealed the following: Protein content of 3.4 g/100 g, fat content of 2.7 g/100 g, non-fat milk solids of 9.1 g/100 g, acidity of 65.5°T, lactic acid bacteria count of 1.9×10⁸ CFU/g, mold count<1.0 CFU/g, and no pathogenic bacteria were detected. The quality meets all the requirements specified in GB 19302—2025 National food safety standard for fermented milk.
This study optimize a composite-flavored yogurt that combines the aromas of sweet potato and fruit, providing feasible process parameters and data support for the industrial production of multi-flavored fermented milk. It holds significant importance for the deep processing of specialty fruit and vegetable raw materials and the expansion of the industrial chain.
To establish a rapid model for varietal discrimination of Chinese red wines by leveraging the differences in antioxidant activity among different varieties.
The total phenolic content, total flavonoid content, cupric ion reducing antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl radical scavenging capacity and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt radical cation scavenging capacity of different varieties of Chinese red wines (Pinot Noir, Merlot, Cabernet Gernischt and Cabernet Sauvignon) were determined and compared. Meanwhile, principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and support vector machine (SVM) algorithms were combined to establish a varietal identification model for Chinese red wines.
Comparative analysis revealed that the order of antioxidant activity of the 4 varieties was: Cabernet Sauvignon>Merlot>Cabernet Gernischt>Pinot Noir. On this basis, the constructed PCA (R2Xcum=0.819) and OPLS-DA (R2Xcum=0.952, R2Ycum=0.424, Q2cum=0.387) models effectively characterized the data features and intergroup differences. The SVM varietal identification model achieved a cross-validation accuracy of 94.40%, with 100.00% accuracy in the training set and 96.26% accuracy in the test set.
Based on the variety-specific antioxidant activity differences of Chinese red wines, combined with multivariate statistical methods and machine learning algorithms, rapid and accurate discrimination of Chinese red wine varieties can be effectively achieved.
To establish a method to determine the quality of safflower yellow in different manufacturers by high performance liquid chromatography (HPLC) fingerprint chromatogram method, and perfect the quality evaluation system of safflower yellow extract.
HPLC analysis was carried out on a C18 chromatographic column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.05% trifluoroacetic acid aqueous solution with gradient elution. The flow rate was set at 1.0 mL/min, column temperature at 30 ℃,detection wavelength at 266 nm, and injection volume at 10 μL. The 16 batches of safflower yellow extract were systematically classified and analyzed via similarity evaluation, cluster analysis and principal component analysis;meanwhile, the content of hydroxysafflor yellow A was determined.
The 11 common characteristic peaks were identified in safflower yellow extract, and 6 of these peaks were assigned. Cluster analysis and principal component analysis demonstrated distinct classification differences among samples from different manufacturers.Orthogonal partial least squares-discriminant analysis (OPLS-DA) screened out 5 major marker components with variable importance in the projection (VIP) values>1; these components exhibited significant fluctuations in products from different manufacturers and could serve as key quality indicators. In terms of hydroxysafflor yellow A content,the quality of commercially available safflower yellow extract was uneven, which might be attributed to the planting environment of raw materials, harvesting and processing techniques, as well as storage conditions.
This study established a comprehensive quality evaluation system combining HPLC fingerprint analysis, chemometrics,and marker component quantification, which provides a scientific basis for refining the quality evaluation system and revising the quality standards of safflower yellow extract.
To study the effects of the krill oil and oyster soft capsules on bone mineral density, bone metabolism and inflammatory state in ovariectomized rats.
The ovariectomized rat models were established and divided into sham operation group, ovariectomized control group, low, medium and high dose of krill oil and oyster soft capsule groups and calcium carbonate group, which were continuously administered for 3 months.The body weight data were recorded weekly. At the end of the experiment, the changes in body weight, femoral bone calcium content, bone mineral density of the middle and distal femur, and the expression levels of osteoprotegerin(OPG)-receptor activator of nuclear factor kappa-B (RANK)-RANK ligand (RANKL) signaling system related molecules in serum were measured.
The low, medium and high doses of krill oil and oyster soft capsules significantly increased femoral bone calcium content and bone mineral density of distal femur in ovariectomized rats;middle and high doses of krill oil and oyster soft capsules could significantly increase bone mineral density of middle femur, which was not lower than that of calcium carbonate treatment group. The results of bone metabolism markers showed that the expression levels of alkaline phosphatase, osteocalcin and procollagen type I amino acid propeptide significantly increased in the high dose group of kril oil and oyster soft capsules, while the expression levels of bone alkaline phosphatase and C-telopeptide of type I collagen were significantly inhibited in the low, medium and high dose groups. The results of OPG-RANKL-RANK signaling system related molecular detection showed that the low-dose kril oil and oyster soft capsules significantly inhibited the levels of osteoprotegerin, and the medium and high dose significantly inhibited the levels of osteoprotegerin, interleukin-6 and interleukin-8 in serum of ovariectomized rats, and significantly increased the level of transforming growth factor-β1.
The krill oil and oyster soft capsules can improve bone mineral density, bone metabolism and ameliorate inflammatory state in ovariectomized rats through multiple mechanisms such as providing calcium source, maintaining the balance between bone formation and bone resorption, anti-inflammation, regulating OPG-RANKL-RANK signaling system, and reducing high bone turnover rate.
The establishment of a standard system for pesticide residues in animal-derived foods plays a vital role in protecting public health and facilitating international trade. This study systematically reviewed the current development of maximum residue limit standards for pesticides in animal-derived foods in China, conducted a comparative investigation into the relevant standard systems established by the Codex Alimentarius Commission(CAC), the European Union, the United States, Japan, and summarized the achievements in the construction of China's standard system. The total number of limit indicators for animal-derived foods in China has increased by 20 times since 2016. Compatibility with international standards (particularly CAC standards) has been continuously enhanced, while monitoring and risk assessment capabilities have steadily improved. At the same time, China still faces challenges such as insufficient coverage of standards and supporting detection methods, the absence of guidelines for pesticide residue definition, insufficient localized risk assessment technologies, and the need to further improve adaptability to international standards and actual trade practices. Therefore, future efforts should focus on:expanding standard coverage to fill gaps for special livestock and poultry products, aquatic products, imported foods and other categories; accelerating the research, development and standardization of supporting detection methods;strengthening research on localized risk assessment technologies suitable for China’s national conditions; clarifying guidelines for the definition of pesticide residues in China; deepening international coordination to enhance the trade compatibility of the standard system. This study aims to further improve the scientific, comprehensive and up-to-date standard system for pesticide residues in animal-derived foods, so as to provide solid theoretical support for enhancing food safety governance in China.
In recent years, with the continuous development and utilization of characteristic products such as Panax ginseng flower tea and Panax notoginseng flower beverages, as well as the rapid growth of the functional food sector, research on flowers of Panax species has attracted increasing attention. As an important component of Panax plants, these flowers are rich in diverse bioactive compounds, including ginsenosides, polyphenols, flavonoids,polysaccharides and volatile constituents, and exhibit various biological activities such as antioxidant,anti-inflammatory, immunomodulatory, neuroprotective and anti-fatigue effects. This review systematically summarized recent advances in the chemical composition, biological activities and underlying mechanisms of Panax species flowers (including Panax notoginseng flower, Panax ginseng flower and Panax quinquefolius flower). It further focused on the current applications and advantages of different flower resources in functional food development, and compared the relationships between their bioactive components and health benefits. In addition, it discussed future perspectives on the high-value utilization of Panax flower resources, the development of functional products and applications in precision nutrition, aiming to provide theoretical references and scientific support for both fundamental research and industrial applications in this field.
To investigate the effects of glucose fermentation products with other raw materials on the quality of pickled vegetables during storage at 37 ℃.
The 3 kinds of food raw materials—glucose fermentation products, vinegar powder and soybean dietary fiber were selected. And the pickled radish was as the test object. The effects on the sensory quality and shelf life of pickled vegetables with three food materials used individually were investigated through single-factor experiment. The optimal physical mixing ratio had been determined through an L9(34) orthogonal array design.
When 3 kinds of materials were used alone, the sensory quality of pickled vegetables was improved effectively and aerobic plate count was suppressed. The combination of 3 kinds of materials was 1.5 g/kg of glucose fermentation products, 1.5 g/kg of vinegar powder and 4.0 g/kg of soybean dietary fiber. The pickled vegetables were stored for 24 days at 37 ℃ with satisfactory sensory quality, aerobic plate count no more than 5.88 lg(CFU/g), coliforms no more than 2.99 lg(CFU/g) and amino acid nitrogen no less than 0.12 g/kg. The shelf life achieved was the same as that reached by using sodium dehydroacetate and superior to that achieved by using potassium sorbate.
Various qualities of pickled vegetables are effectively enhanced during their shelf life with the use of glucose fermentation products, vinegar powder and soybean dietary fiber. The common chemical preservatives are replaced to inhibit the growth of spoilage microorganisms. The reliable solution is provided for the green upgrading of the pickled vegetable industry.
To optimize the processing technology of green jujube pulp and investigate its change s in active substances and antioxidant activity.
Shanxi Banzao was used as the raw material, with direct pulping as the traditional process reference, and the effects of direct pulping, microwave-assisted pulping, and ultrasonic-assisted pulping on the flavonoids and polyphenols content in the pulp were compared to determine the optimal pulping method. A single-factor and orthogonal experimental design was employed to optimize the ultrasonic-enzymatic hydrolysis composite process, and the content of active components and antioxidant activity in the pulp were measured. Subsequently, the active component content and antioxidant properties of 9 kinds of Shanxi green jujube pulps were compared before and after process optimization, and high-quality varieties were selected through standardized relative value heat map analysis.
Ultrasonic-assisted pulping significantly increased the polyphenol content in the pulp and was identified as the optimal method. The optimized ultrasonic-enzymatic hydrolysis parameters were determined as follows: Ultrasonic power 450 W, feed-to-pulp ratio 1:20 (m:V), and enzyme addition rate 0.7%. Under these conditions, the total flavonoid content in Banzao pulp reached 392.46±5.43 mg/100 g, while the half maximal inhibitory concentration values for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) scavenging capacities were as low as 4.51 mg/mL and 15.9 mg/mL, respectively. The total vitamin C and dietary fiber contents increased by 25.2% and 0.8%, respectively.Heat map analysis identified 3 green jujube varieties-Banzao, Youzao and Tuntun-as those with the highest overall quality.
This study optimized and established the optimal production process for green jujub pulp, and the content of its active ingredients and antioxidant activity are effectively enhanced. The findings provide theoretical support for optimizing the processing techniques and selecting suitable varieties of green jujube pulps, which demonstrate significant practical value and potential for widespread application.
Fish processing by-products are rich in collagen, myofibrillar proteins and endogenous enzymes, and are valuable protein resources for the preparation of natural antioxidant peptides. Recent studies have paid increasing attention to the effects of preparation processes on peptide structure and antioxidant activity, and have extended activity evaluation to food matrices. This review summarized the research progress of antioxidant peptides derived from fish skin, scales, bones, heads, viscera and swim bladders, with emphasis on the effects of enzymatic hydrolysis,microbial fermentation and ultrasound-assisted hydrolysis on molecular weight distribution, exposured of active residues and peptide sequence composition. The structure-activity relationships were discussed in terms of molecular weight, amino acid composition, sequence position, secondary structure and interfacial action in food systems. In summary, this review provides a theoretical basis for the high-value utilization of fish processing by-products, the targeted preparation of antioxidant peptides, and their applications in food antioxidation and preservation.