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Objective To compare the similarities and differences in the identification results of Staphylococcus aureus in dried fruits analyzed by 3 experimental methods: Biochemical identification, mass spectrometer identification and molecular biological identification. Methods Staphylococcus aureus in dried fruits was isolated. The isolated typical strains were identified and compared through 3 approaches: Biochemistry (VITEK 2-Compact) mass spectrometer (VITEK MS), and molecular biology (real-time fluorescence quantitative polymerase chain reaction), the identification results of the 3 types of experiments were compared. Results All 3 identification methods could identify Staphylococcus aureus to the species level, but there were certain differences in efficiency and time required. Mass spectrometry yielded more accurate results with higher timeliness. Conclusion This paper analyzes the advantages and disadvantages of different identification methods, combines traditional methods with modern technologies. The enrichment and isolation in traditional detection methods provide a basis for subsequent identification. On this basis, advanced identification technologies with different principles are integrated to form a complete detection process. This combined mode can mutually verify the results, improve the detection accuracy, and provide new ideas for establishing a more reliable microbiological detection system for characteristic dried fruits.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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