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Determination of scopolamine, racanisodamine and atropine in Datura stramonium Linn. by liquid chromatography-tandem mass spectrometry
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Shu-Ling DENG1, Feng ZHU2, Hao ZHANG2, Zhong-Li HUO2, Lian-Hong WANG3, *, Hua-Liang LIU1, 2, *
Journal of Food Safety & Quality | 2025, 16(11) : 131 - 139
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Journal of Food Safety & Quality | 2025, 16(11): 131-139
• Special Topic: Analysis and Monitoring of Toxic and Harmful Substances in Food •
Determination of scopolamine, racanisodamine and atropine in Datura stramonium Linn. by liquid chromatography-tandem mass spectrometry
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Shu-Ling DENG1, Feng ZHU2, Hao ZHANG2, Zhong-Li HUO2, Lian-Hong WANG3, *, Hua-Liang LIU1, 2, *
Affiliations
  • 1. School of Public Health, Nanjing Medical University, Nanjing 211166, China
  • 2. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China
  • 3. State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing 210023, China
Published: 2025-06-15 doi: 10.19812/j.cnki.jfsq11-5956/ts.20250228003
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Objective To establish a method for the rapid extraction of Datura stramonium Linn. seeds, Datura stramonium Linn. flowers, Datura stramonium Linn. shells, Datura stramonium Linn. leaves and Datura stramonium Linn. stems and simultaneous determination of scopolamine, racanisodamine and atropine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods The Datura stramonium Linn. seeds were extracted with methanol, flowers were extracted with ethanol and others were extracted with 1% formic acid solution. An ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) column was used for the qualitative and quantitative analysis of scopolamine, racanisodamine and atropine by external standard method in multiple reaction monitoring (MRM) mode using 0.1% formic acid aqueous solution-methanol as the mobile phase in a gradient elution and electrospray ionization with a positive ion source. Results Scopolamine, racanisodamine and atropine showed good linearity (r≥0.999) in the range of mass concentrations from 0.1 to 10.0 ng/mL. Limits of detection were 30-76 μg/kg, limits of quantitation were 80-253 μg/kg. The average recoveries of the 3 alkaloids at the low, medium and high spiked concentration levels ranged from were 81.9%-117.0%, and the relative standard deviations (RSDs) were 0.7%-7.2%. Conclusion The method is simple, rapid, accurate, can provide reliable technical support for the determination of scopolamine, racanisodamine and atropine in Datura stramonium Linn..

liquid chromatography-tandem mass spectrometry  /  Datura stramonium Linn.  /  scopolamine  /  racanisodamine  /  atropine
Shu-Ling DENG, Feng ZHU, Hao ZHANG, Zhong-Li HUO, Lian-Hong WANG, Hua-Liang LIU. Determination of scopolamine, racanisodamine and atropine in Datura stramonium Linn. by liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety & Quality, 2025 , 16 (11) : 131 -139 . DOI: 10.19812/j.cnki.jfsq11-5956/ts.20250228003
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Year 2025 volume 16 Issue 11
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Article Info
doi: 10.19812/j.cnki.jfsq11-5956/ts.20250228003
  • Receive Date：2025-02-28
  • Online Date：2025-07-14
  • Published：2025-06-15
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History
  • Received：2025-02-28
Funding
Affiliations
    1. School of Public Health, Nanjing Medical University, Nanjing 211166, China
    2. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China
    3. State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing 210023, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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