With the rapid development of the Chinese economy, coffee consumption in China has rapidly expanded, and the processing scale of coffee and related products in China has grown significantly in recent years. However, many food safety risks caused by non-standard processing in the industry have also been exposed as the industry grows. Among them, risks such as excessive use of food additives beyond the scope, excessive mold contamination caused by poor initial processing hygiene conditions, and extreme emphasis on flavor and neglect of acrylamide contamination in baking are more prominent. These risks will be amplified with the surge in consumption. Therefore, it is urgent to introduce normative documents to effectively guide the industry to control non-standard work. At present, China has not yet issued national standards or other authoritative documents on risk control measures for coffee processing exposure, which is significantly different from international standards. This article focused on the main food safety risks currently present in the coffee processing industry, and provided some perspectives and suggestions for future regulatory documents on coffee processing. The significance of this article is to standardize the processing of the coffee industry, ensure effective supervision, strictly control illegal additives, and suppress fraudulent behavior, providing standard support for improving the rate of high-quality coffee.

Objective To evaluate the food safety and quality of prepared dishes sold in Guangdong Province from 2021 to 2023. Methods According to the national definition and scope of prepared dishes, and considering their 5 main characteristics, relevant data were selected from the food supervision and sampling inspection database of Guangdong Province from 2021 to 2023. Additionally, 20 batches of frozen meat products were randomly purchased from supermarkets and stores within the province for animal-derived component testing. The results were evaluated based on food safety national standards and statistically analyzed using WPS Office 2024. Results The supervision sampling inspection data of prepared dishes sold in Guangdong Province from 2021 to 2023 covered 8910 batches and 20019 items, with 18 testing items including physicochemical indicators, contaminants, microorganisms, and animal-derived components. The compliance rate for all items except the microorganism indicator in meat products exceeded 99%. Among the 20 randomly purchased frozen meat products, 15 batches were detected to contain other animal-derived components. By analyzing the above data, several issues were identified in prepared dishes: Food classification could be easily confused; some products lack evaluation criteria for contaminants; microbial contamination was of concern; and the detection rate of other animal-derived components in beef products was high. Conclusion Overall, the food safety of prepared dishes in Guangdong Province is satisfactory. To address the identified issues, effective control measures should be implemented. This study proposes standardizing the classification criteria for supervision and sampling inspection of prepared dishes, expediting the release of national food safety standards, enhancing the prevention and control of microbial contamination, and strengthening the management of raw material authenticity to promote the healthy development of the prepared dishes industry.

Objective To investigate the pollution of 5 kinds of heavy metals in 4 types of food in Hubei Province from 2022 to 2023, and to assess the health risks caused by heavy metal intake in the population. Methods From 2022 to 2023, a total of 316 samples of 4 types 6 kinds of food (soybean, peanut, mushroom, dictyophora, rice and wheat) were randomly collected in Hubei Province. The content of 5 kinds of heavy metals were detected by inductively coupled plasma-mass spectrometry (ICP-MS), and the contamination of food was analyzed by single factor pollution index (P_i) and comprehensive factor pollution index (P_M). According to the health risk assessment model recommended by U.S. Environmental Protection Agency, the target hazard quotient (THQ) and target cancer risk assessment (TCR) were used to assess the non-carcinogenic and carcinogenic health risks caused by heavy metal exposure in the population. Results The overall qualified rate of food was close to 95%, the detection rate of Cd in heavy metals was the highest (100.0%, 14.2%). There were statistically significant differences in the detection rate and excess rate of the 5 kinds of heavy metals (χ²=179.320, 21.849, P<0.001). The 6 kinds of food contamination levels were dictyophora(severe), shiitake mushroom (mild), soybean (safe), peanut (safe), rice (safe), and wheat (safe). The individual heavy metal THQ of different regions and different genders was less than 1, but the comprehensive heavy metal THQ was greater than 1, and the carcinogenic risk of heavy metal in different regions and different genders was greater than 10^-4. In Hubei, the non-carcinogenic and carcinogenic health risks caused by dietary heavy metals intake were higher in rural areas than in urban areas, and higher in women than in men. Non-carcinogenic health risks mainly come from Cd and Cr, while carcinogenic health risks mainly come from Cr. Conclusion There is a certain degree of contamination of edible fungi in the four types of food sold in Hubei Province from 2022 to 2023, especially the heavy pollution of dictyophora. The non-carcinogenic health risk caused by the intake of heavy metals through dietary means is high, and the carcinogenic risk is within the acceptable range. Relevant departments should strengthen supervision and pay attention to the health risks of Cd and Cr exposure.

Objective To establish a new non-targeted screening approach for multi-class veterinary drugs in fish based on metabolomics analysis. Methods High performance liquid chromatography-tandem mass spectrometer in full scan mode was adopted for the determination of fish to obtain the metabolomics data, which were uploaded onto Workflow4Metabolomics platform. The data underwent chromatographic peak treatment to obtain a data matrix containing 867 variables. The data matrix was further analyzed by principal component analysis, cluster analysis, orthogonal partial least squares discriminant analysis, pairwise t-test and fold change of concentration to screen eligible variables. These variables were confirmed as the characteristic ones of marker compounds to represent residual veterinary drugs via comparison within a compound database. Results The 88 in 102 kinds of veterinary drugs were screened by proposed metabolomics approach under the help of high performance liquid chromatography- tandem mass spectrometer (HPLC-MS/MS) positive mode, with the limits of detection ranging from 0.3 to 2.6 µg/kg, achieving a screening rate of 86%. Conclusion Metabolomics analytical strategy can effectively realize the non-targeted screening of veterinary drugs in fish, and provides a new idea for non-targeted screening of contaminants.

Objective To establish a method for qualitative determination of Tricholoma matsutake ingredients by a TaqMan real-time fluorescence polymerase chain reaction (PCR), and identify the conformity of Tricholoma matsutake and its products with their labels by DNA barcoding technology. Methods Taking the pol gene of Tricholoma matsutake as the target gene, specific primers and probes were designed to study and analyze their specificity, sensitivity and repeatability. The established TaqMan real-time PCR method of Tricholoma matsutake was used to detect different types of commercially available samples and identify their authenticity. Results The established method was used for real-time PCR detection, and there was no cross-reactivity between Tricholoma matsutake and 32 kinds of other edible fungi, animals and plants, indicading strong specificity of the method. The sensitivity was 0.01% Tricholoma matsutake and 0.01 ng/μL Tricholoma matsutake genomic DNA. The authenticity test of 160 commercially available Tricholoma matsutake and their products showed that there was counterfeiting of dried Tricholoma matsutake. The test results did not match the labels at a rate of 36.70%. DNA barcoding technology was used to further identify the species of counterfeit matsutake mushrooms, with species attributes of Stropharia rugosoannulata, Tricholoma giganteum and Tricholoma bakamatsutake. The non-compliance rates with labels in the authenticity testing resultes of other collected matsutake products were 23.70%-60.00%, respectively. Conclusion This method has high sensitivity and strong specificity, which can quickly and accurately identify the authenticity and compliance of Tricholoma matsutake ingredients.

Objective To analyze the differences in mineral elements in wild rice from different regions and establish a method for tracing the origin of wild rice based on mineral element fingerprint characteristics. Methods Inductively coupled plasma mass spectrometry (ICP-MS) was used to analyze the content of 24 kinds of mineral elements in 40 wild rice samples from the United States, Canada, and China. The data were subjected to variance analysis, multiple analysis, principal component analysis, and discriminant analysis to explore the feasibility of using mineral element fingerprint characteristics for tracing the origin of wild rice. Results The content of 24 kinds of elements showed significant differences among the regions. Principal component analysis extracted 3 principal components, with a cumulative variance contribution rate of 93.1%. Principal component analysis successfully distinguished wild rice from the United States and Canada. Discriminant analysis correctly identified 97.7% of the wild rice samples. Conclusion This study demonstrates the feasibility and accuracy of classifying wild rice samples from different origins.

Objective To investigate the variation in heavy metal elements content in Mizuhopecten yessoensis and Anadara broughtonii from the Dalian Region. Methods Samples of Mizuhopecten yessoensis and Anadara broughtonii were collected monthly over the course of a year in Dalian. The content of heavy metal elements, including Pb, Cd, Cr, and Cu, were determined using inductively coupled plasma mass spectrometry. The study examined the monthly trends in the accumulation of these elements in the 2 types of shellfish. The single-factor pollution index method and the target hazard quotient method were used to evaluate the consumption risk and potential health risks to humans from consuming shellfish products with accumulated heavy metals. Results The Cd content in Mizuhopecten yessoensis peaked in March, with the highest detected value reaching 4.50 mg/kg, exceeding the national food safety standard limit. The Cu content in Mizuhopecten yessoensi was significantly higher in March, April, and October compared to other months, but all annual detection values were below the limit. In Anadara broughtonii, the Cd content peaked in March and April, with the highest detected value being 1.92 mg/kg, but no significant variation pattern was observed in other months, and all annual detection values were below the limit. The content of other heavy metal elements did not show significant variation patterns over time or seasons. Conclusion The heavy metal content in Mizuhopecten yessoensis shows the most significant monthly variation. For Cd, Mizuhopecten yessoensis are contaminated in March, April, and May, posing health risks if consumed. Anadara broughtonii remaines within safe consumption levels throughout the year, with lower health risks.

Objective To investigate the pollution status of perfluorinated compounds (PFCs) in commercial fish in Liaoning coastal areas. Methods The 6 species of marine commercial fish were collected from 4 coastal areas in Liaoning, including Lvshun, Zhuanghe, Yingkou, and Jinzhou. A standardized pre-processing procedure for marine fish samples was established. PFCs were detected using liquid chromatography-tandem mass spectrometry. The pollution levels of PFCs in the commercial fish were assessed, and the bioaccumulation risk analysis was conducted. Results The main PFCs pollutants in economic fish species in sea were perfluorooctanoic acid (PFOA) and perfluorooctane sulfonates (PFOS). PFOA was detected in all fish samples, with maximum and minimum concentrations of 30.30 ng/g ww and 4.02 ng/g ww, respectively. PFOS was detected in 100% of fish viscera samples but was not found in muscle tissues. Among the tested species, Sebastodes fuscescens, Hexagrammos otakii, and Larimichthys polyactis were more prone to accumulating PFOS and PFOA, indicating potential bioaccumulation effects. Conclusion PFCs contamination is widespread in commercial fish. Continuous monitoring of PFCs shall be implemented, and control measures shall be taken in areas with severe PFOS and PFOA pollution. Ongoing monitoring and risk analysis of these pollutants are essential.

As the cornerstone of the global food system, wheat flour, a derivative product of limited wheat in various countries, is not only an important component of human daily diet, but also a key element in maintaining global food security. However, in recent years, the frequent contamination of fungal toxins in wheat flour has become a major challenge in the field of food safety, posing a significant threat to food safety and human health. The main types of fungal toxins in wheat flour include deoxynivalenol, aflatoxin, zearalenone, etc. The contamination of deoxynivalenol is the most prominent, but there are certain differences in the relevant limit standards at home and abroad. This study aimed to comprehensively review the types and contamination status of major fungal toxins in wheat flour. By comparing relevant limit standards at home and abroad, it could be found that different countries and regions had different assessment and management strategies for food safety risks. Based on the analysis and discussion of the above content, a comprehensive prevention and control strategy is proposed, aiming to provide scientific guidance and technical support for the safe production, processing, and consumption of wheat flour.

Objective To investigate the generation pattern of heterocyclic amines (HAs) and advanced glycation end products (AGEs) in plant hamburger meat under different thermal processing conditions. Methods ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to analysed the effects of different treatments (uncooked, steamed and grilled), grilling temperatures (160, 180, 200 °C), and grilling times (4, 8, 12 min) on the content of free HAs and AGEs, bound HAs and AGEs, and amino acids in the plant hamburger meat. AGEs and amino acid content of plant hamburger meat, and investigate the simultaneous generation of free and bound HAs and AGEs in plant hamburger meat under different thermal processing conditions using principal component analysis (PCA). Results The results of PCA showed that the highest levels of free HAs and AGEs and bound HAs were found in plant hamburger meat under 200 °C/12 min roasting condition in the roasted sample group. A total of (11.44±2.68) ng/g of total free HAs, (80.24±9.56) ng/g of total bound HAs, (1.99±0.29) μg/g of total free AGEs and (46.00±4.00) μg/g of total bound AGEs were detected in the raw hamburger meat. Compared with raw plant hamburger meat, there was an increase in bound N^ε-carboxyethyl-lysine (CELs) and a smaller difference in free AGEs and HAs in steamed plant hamburger meat. There was a significant increase in hazardous material content after steaming and roasting (200 °C, 12 min), which increased by 191.83%, 164.38%, 218.59% and 15.70%, respectively, as compared to the blank group. During roasting, the free and bound HAs, free AGEs in plant hamburger meat showed a increase with the increase of roasting temperature and time. The total amount of free and bound HAs and free AGEs increased by 79.14%, 23.38% and 88.05%, respectively, under the conditions of roasting temperature of 180 °C and roasting time of 4-12 min; under the condition of roasting time of 8 min, the content of hazards in plant hamburger meat at roasting temperature of 200 °C compared with that at 160 °C increased by 61.28%, 34.82% and 67.93%, respectively. Conclusion Different treatments and thermal processing conditions has significant effects on the generation of HAs and AGEs, which increase with the increase of roasting temperature and the prolongation of roasting time in plant hamburger meat. This study aims to provide theoretical and experimental basis for the reduction and control of HAs and AGEs in thermal processing of plant hamburger meat.