Anthracnose is prevalent in major oil-tea production regions across the country, severely compromising yield and quality of oil-tea Camellia oleifera. Colletotrichum fructicola is the dominant pathogenic fungus responsible for this disease, and understanding its pathogenic mechanisms is fundamental for controlling Ca. oleifera anthracnose. In eukaryotes, autophagy is a conserved intracellular degradation pathway and serves as the primary route for degrading macromolecular proteins and damaged organelles. Atg7, a core autophagy protein, plays crucial roles in many plant-pathogenic fungi. However, its biological functions in Co. fructicola remains unclear. In this study, the CfATG7 gene knockout mutant ΔCfatg7 and its complementary strain ΔCfatg7/CfATG7 were constructed, and their phenotype and pathogenicity were assayed. The results demonstrate that CfAtg7 is involved in regulating autophagy, vegetative growth, conidiation, and appressorium formation. It also participates in the responses to endoplasmic reticulum stress, cell wall integrity stress, and oxidative stress, revealing the pleiotropic roles of the autophagy protein CfAtg7 in Co. fructicola. This study elucidated the biological functions of CfAtg7 in Co. fructicola, providing experimental evidence for the development of novel fungicides targeting this protein.

A pure culture strain of a wild fungus from Zhouqu County of Gannan Tibetan Autonomous Prefecture in Gansu Province was obtained by tissue separation method. Morphological observation and ITS sequence analysis confirmed that the fungus was Trametes betulina, and its biological characteristics and domestication cultivation were studied. The results of biological characteristic investigation showed that the optimum carbon source was fructose, and the nitrogen source was yeast powder. The mycelia grew well under pH 6.0 and temperature of 32 ℃. The orthogonal test showed that the main factor affecting mycelial growth was temperature, and pH, nitrogen source and carbon source were secondary. The results of domestication cultivation experiments showed that the best spawn culture medium was: wheat grain 20%, sawdust 70%, bran coat 7%, gypsum 2%, lime 1%. The best expanded cultivation substrate formula was: wheat grain 25%, sawdust 25%, cottonseed hull 20%, corncob 20%, wheat bran 7%, sugar 1%, gypsum 1%, lime 1%. After inoculation, fruiting body could be harvested in 26 days under 25 ℃ condition. The total yield of fresh weight was 144.54 g/bag. The antioxidant activity of ultrasonic water extract of cultivated fruiting body increased with the increase of additional quantity of the extract. When the additional quantity of the extract was 5 mg/mL, the DPPH and hydroxyl radical scavenging rates were the highest, reaching 95.69% and 71.59%, respectively, while the ABTS⁺ scavenging rate peaked at 0.25 mg/mL, reaching 98.19%. The results provide a reference for the development and utilization of wild germplasm resource of Trametes betulina in China.

Hericium erinaceus polysaccharides as the main active component extracted from H. erinaceus have many biological activities such as anti-oxidation, anti-tumor, and immune regulation. However, there are some limitations in solubility, bioavailability and activity intensity of natural H. erinaceus polysaccharides. In recent years, certain progress has been made in the studies of chemical modification, and structural features, having effects on the biological activities of H. erinaceus polysaccharides. In this paper, the recent advances in the study of chemical modification and biological activities of H. erinaceus polysaccharides are summarized, and the chemical modification methods of H. erinaceus polysaccharides through introducing new functional groups to substitute hydroxyl groups on the polysaccharide chains are elucidated. The changes in the structure and physicochemical properties of the chemically modified H erinaceus polysaccharides are introduced, and the alterations in biological activities and the underlying mechanisms of the chemically modified H. erinaceus polysaccharides are analyzed and discussed. It is expected that this work might provide a theoretical basis for further exploration of modification of H. erinaceus polysaccharides.

Physarum subgalbeum sp. nov. is described through observation of integrated morphological characterization and multigene phylogenetic analyses (nrSSU, mtSSU, α-Tub, and COI). The life cycle of P. subgalbeum was investigated in water agar medium and microscopic structure formation at different stages during sporulation was observed. This species is similar to P. galbeum in having golden yellow sporocarps. However, it differs in that its sporocarps often fuse into clusters, the spore surface is densely covered with small spines, and the lime nodes are elongated fusiform. Phylogenetic analysis results indicate that P. subgalbeum forms an independent monophyletic clade and constitutes a sister group relationship with P. galbeum. The spore germination of P. subgalbeum is split type, the plasmodial type is of phaneroplasmodium and sporulation requires approximately 10-12 hours, with lime nodes and capillitium forming prior to spore maturation. A comprehensive morphological description, including detailed micrographs, results of phylogenetic analyses, as well as a thorough account of its life cycle accompanied by corresponding microscopic images are provided.

The new Flammulina filiformis cultivar ‘Shangyan 52’ was bred by multi-spore inbreeding using the strain ‘Shangyan 1’ as parent. The basidiospores of the parent strains were mutated by normal atmospheric pressure and room temperature plasma mutagenesis. The fruiting body of ‘Shangyan 52’ is white, with numerous buds; the pileus is buckled and hemispheric, and the stipe is long. The new cultivar ‘Shangyan 76’ was bred by multi-spore inbreeding using the strain ‘T011’ as parent. The basidiospores of the parent strains were also mutated by normal atmospheric pressure and room temperature plasma mutagenesis. The fruiting body of ‘Shangyan 76’ is white; the pileus is small, thick, buckled and hemispheric, and the stipe is short and thin. The new cultivar ‘Shangyan 84’ was bred by single spore crossbreeding using the strains ‘Shangyan 1’ and ‘T011’ as parents. The fruiting body of ‘Shangyan 84’ is white, with numerous buds; the pileus is large, buckled and hemispheric, and the stipe is thick. There is no significant difference in the growth period of ‘Shangyan 52’ and ‘Shangyan 84’ and the main cultivar, and the growth period of ‘Shangyan 76’ is 2-3 days longer than that of the main cultivar.

Phlebopus portentosus, the first successfully domesticated and cultivated bolete species, has undergone rapid industrial expansion in recent years, highlighting the growing need for accurate strain identification and germplasm protection. In this study, whole-genome resequencing was performed on 66 strains of P. portentosus collected from various regions, resulting in the construction of a database of multiple nucleotide polymorphism (MNP) markers containing 306 loci. Population structure and kinship analyses based on both single nucleotide polymorphisms (SNPs) and MNPs consistently grouped the strains into two distinct genetic clusters. The MNP markers exhibited high resolution and stability across successive subculturing generations, enabling precise strain discrimination. Clonal identification criteria were established using replicate sequencing data of the same strains, with thresholds set at PI_HAT>0.95 and genetic distance (D)<0.01, while a genetic similarity (GS) value of 100% within the MNP dataset was validated as a robust indicator of strain identity. Additionally, machine learning approaches were employed to identify key MNP loci indicative of population divergence, offering valuable markers for varietal authentication and functional genomics. This work established MNP molecular marker database for P. portentosus and validated the potential applications in genetic resource conservation and molecular breeding, providing scientific support for variety protection and germplasm innovation.

Buchwaldoboletus xylophilus is the second cultivatable species of Boletales with significant scientific and commercial potential value. To better develop and utilize this wild resource, the morphological and molecular identification and the biological characteristic investigation of a wild strain, JSJ-Bx1, were carried out. The results show that this wild strain belongs to B. xylophilus. The hyphae grow well under the temperature of 35℃ and pH 4.0 on the medium using glucose as carbon source, and yeast extract as nitrogen source with the addition of inorganic salt FeSO₄. The optimal medium formula consists of 20 g glucose, 2 g yeast extract, 1.5 g FeSO₄, 0.3 g 1/2 MS medium, 20 g agar, and 1 L water. The hypha growth rate is 11.38 mm/d. The optimal expanded cultivation substrate formula consists of 20% sawdust, 30% cottonseed hulls, 20% wheat grains, 10% corn flour, 10% red soil, 8% bran, 1% gypsum powder, 1% glucose, 0.1% KH₂SO₄, and 0.1% FeSO₄. The mycelial colonization period is about 20 days; primordial formation takes about 5 days, and the growth and maturation of the fruiting bodies takes about 7 days. The average yield is 96.64 g/bottle and the biological efficiency is 31.58%.

In total, 310 isolates of non-albicans yeast (NAY) recovered from vulvovaginal candidiasis (VVC) patients were collected and identified at species level, and antifungal susceptibility of the isolates was determined. Literature relating to non-albicans yeast (NAY) species distribution in recent 10 years was searched and 7 publications were included in this study. Data of the literature together with the data of this study indicated that spectrum of NAY causing VVC shifted significantly. Nakaseomyces glabratus is the predominant species among NAY, responsible for 41.34%-80.43% of vaginal NAY infection. The other NAY species revealed characteristics of regional distribution. For example, Candida parapsilosis (Portugal 23.74%, UK 20.38%) and Pichia kudriavzevii (Serbia 22%, Greece 11.30%) dominated higher ratio in Europe, while C. tropicalis showed higher ratio in China and Greece (both 7.40%). Rare yeast mainly consist of species of saccharomycotina. They are diverse and distinctly different in distribution pattern between China and Europe. Fluconazole is still optimal oral treatment option in China, and clotrimazole and amphotericin B could be precedent choices as topical drugs for treating VVC caused by NAY. Increase of rare yeast would be a challenge for clinical treatment of VVC. Correct identification at species level and determination of susceptibility to antifungals are necessary.

Lentinula edodes polysaccharides exhibit diverse biological activities. Currently, most L. edodes polysaccharides are extracted from fruiting bodies, resulting in low yield, high cost, and short harvest period. Therefore, enhancing polysaccharide production has become a research priority. Epigenetic modifications regulate the synthesis and metabolism of secondary metabolites through various mechanisms and play significant roles in microbial secondary metabolism. To investigate whether polysaccharide synthesis in L. edodes is influenced by epigenetics, this study utilized L. edodes strain Xin 808 as experimental material, and analyzed the effects of different concentrations of two epigenetic modifiers [benzamide and sodium carboxymethyl cellulose (CMC-Na)] on mycelial growth, cellulase activity, polysaccharide production, activity of key polysaccharide-synthesizing enzymes, relative expression levels of the enzyme encoding genes, and antioxidant activity of polysaccharides. Results showed that although 4 µg/mL benzamid and CMC-Na reduced biomass compared to the control, they significantly promoted the growth of L. edodes mycelium on solid culture medium and increased the polysaccharide content in the mycelium. Cellulase activity decreased compared to that of the control, but the intracellular crude polysaccharide concentration increased, reaching 2.31-fold (benzamide) and 2.01-fold (CMC-Na) as compared with that of the control. The activities of UDP-glucose pyrophosphorylase (UGPase), phosphoglucose isomerase (PGI), and α-phosphoglucomutase (α-PGM), the enzymes associated with lentinan metabolism, increased. Under benzamide treatment, these enzymes exhibited increases in activity, increasing by 2.21-fold, 2.38-fold, and 2.19-fold as compared with enzyme activities of the control group, respectively. However, the relative expression levels of the encoding genes were significantly downregulated. In vitro polysaccharide antioxidant capacity increased in variant degrees. At the concentration of 0.2 mg/mL ·OH scavenging ability showed the most pronounced. These results demonstrate that adding benzamide and sodium carboxymethyl cellulose at appropriate concentrations influences L. edodes mycelial growth and polysaccharide synthesis.

Wheat dwarf smut, caused by Tilletia controversa (TCK), is a dangerous fungal wheat disease that is highly destructive and difficult to eradication, possessing a significant impact. The disease is known as “No. 1 disease of wheat”, and is listed as a quarantine pest by European Plant Protection Organisation (EPPO), Australia, China and other countries/regions and organizations. Due to difficulties for distinguishing TCK from other closely related species such as Tilletia laevis in levels of morphological characteristics and molecular biology, misdiagnosis easily happen in quarantine inspections. In this study, TCK-specific molecular markers were developed at the genomic level, and four candidate genes, namely OG0008430, OG0008601, OG0008603 and OG0008610, were screened. Eight pairs of primers and four probes based on these four candidate genes were designed. Validation of combinations among the primers and probes were verified, and two sets of “triple + single” real-time fluorescence PCR methods were established. This combined method can effectively distinguish target species (TCK) from closely related species, achieving accurate identification of TCK, and it can also be used to distinguish different species within the genus Tilletia.