To compare the disparities in the modified Kupperman’s score（KMI）and peripheral blood micro indicators between non-interaction between heart and kidney syndrome and liver depression and Qi stagnation syndrome of perimenopausal women experiencing sleep difficulties.

Eighty patients with perimenopausal sleep disorders were selected as research participants using the Pittsburgh Sleep Quality Index（PSQI）. Among them，40 patients had non-interaction between heart and kidney syndrome and 40 patients had liver depression and Qi stagnation syndrome. The data，including traditional Chinese medicine syndrome collection forms and KMI scales was gathered. Enzyme-linked immunosorbent assay（ELISA）was used to quantify serum inflammatory markers［interleukin-1β（IL-1β），tumor necrosis factor-α（TNF-α）］and neurotransmitter levels［gamma aminobutyric acid（GABA），glutamic acid（Glu）］in two groups.

The study findings revealed that noninteraction between heart and kidney syndrome had higher values for time to fall asleep，sleep time，sleep efficiency，sleep disorder score and total PSQI score compared to the liver depression and Qi stagnation syndrome group（P<0.05）. Additionally，the liver depression and Qi stagnation syndrome group had a higher daytime dysfunction score than the non-interaction between heart and kidney syndrome group（P<0.05）. Furthermore，the non-interaction between heart and kidney syndrome group exhibited higher values for hot flashes，sweating，muscle and joint pain，palpitations，urinary system symptoms and total KMI score compared to the liver depression and Qi stagnation syndrome group（P<0.05）. Conversely，the liver depression and Qi stagnation syndrome group had higher scores for emotional excitement，depression，suspicion，dizziness，headache and skin ant sensation compared to the non-interaction between heart and kidney syndrome group（P<0.05）. Serum levels of IL-1βand TNF-αwere also higher in the liver depression and Qi stagnation syndrome group. The levels in the non-interaction between heart and kidney syndrome group were significantly higher than those in the other group（P<0.05）. In contrast，the serum GABA level in the non-interaction between heart and kidney syndrome group was lower than that in the liver depression and Qi stagnation syndrome group，while the Glu level was higher（P<0.05）.

In women experiencing sleep difficulties during the perimenopausal period，the non-interaction between heart and kidney syndrome is associated with worse overall sleep quality compared to the liver depression and Qi stagnation syndrome. Additionally，the non-interaction between heart and kidney syndrome is also linked to more severe perimenopausal symptoms. The sleep disturbances and perimenopausal symptoms have distinct classifications. There are significant differences in the expressions of IL-1β，TNF-α，GABA and Glu between the two syndromes，which may contribute to the objectivity and precision of traditional Chinese medicine syndrome differentiation for sleep disorders in perimenopausal women.

To evaluate the safety and efficacy of Simo Decoction Oral Liquid（四磨汤口服液）for post-prandial distress syndrome（PDS）of functional dyspepsia（FD）.

Randomized，controlled and placebo-controlled parallel trial design was adopted，the observation was conducted in 12 parallel centers，and 240 cases were randomly divided into trial group and control group，with 120 cases in each group. The trial group was administered with Simo Decoction Oral Liquid，while the control group was given Simo Decoction Oral Liquid simulation agent. The treatment course was 4 weeks. The PDSsymptom improvement rate，other individual symptom scores of FD，overall treatment evaluation scale（OTE），hospital anxiety and scale（HAD），functional dyspepsia quality of live（FDDQL）and safety were compared. Full analysis set（FAS）and per protocol set（PPS）were used for effectiveness analysis，while safety set（SS）was used for safety analysis.

There were 237 cases in the FAS（with 118 in the trial group and 119 in the control group），220 cases in the PPS（with 112 in the trial group and 108 in the control group），and 239 cases in the SS（with 120 in the trial group and 119 in the control group）. FASand PPSanalyses showed that after treatment，postprandial distress syndrome，postprandial fullness and discomfort and early satiety in the trial group were higher；the decrease of postprandial discomfort symptoms and postprandial fullness and discomfort VAS scores were larger and the symptom improvement rates of OTEwere higher than those in the control group（P<0.05）. FASanalyses showed that after treatment，the response rates of stomach burning sensation，acid reflux and poor bowel movement in the trial group were higher；the decrease of stomach burning sensation VASscore was larger than those in the control group（P<0.05）. PPS analyses showed that after treatment，the response rates of stomach burning sensation and poor bowel movement in the trial group were higher；the decrease of stomachache，stomach burning sensation and poor bowel movement VAS scores were larger than those in the control group（P<0.05）. There was 1 adverse event occurred in the trial group，and 2 adverse events occurred in the control group，and there were no statistical difference between groups（P>0.05）.

Simo Decoction Oral Liquid is effective and safe in treating post-prandial distress syndrome of functional dyspepsia.

To investigate the mechanism of Tripterygium wilfordii polysaccharides inducing pancreatic stem cell differentiation through stromal cell-derived factor-1（SDF-1）/CXC chemokine receptor-4（CXCR4）axis.

Primary pancreatic stem cells were prepared from pancreatic tissue from Kunming mice on day 4 of neonatal birth. The immunohistochemistry method was used to identify the pancreatic stem cells. Isletβcells were identified by dithiazone（DTZ）staining. An inverted fluorescence microscope was used to photograph and record the cells at various cultivation time points. The release of SDF-1 from cells were detected at various administration time points by using enzyme-linked immunosorbent assay（ELISA）. The relative mRNA expression levels of SDF-1 and CXCR4 in each group were detected by real-time fluorescence quantitative PCR（qPCR）technology. The expression levels of SDF-1 and CXCR4 proteins in each group were detected by Western blot assay（WB）.

The cells obtained was Nestin positive and Brownish-yellow were identified by immunohistochemistry method. DTZ staining was positive，and the number of positive stained cells in the treatment group was significantly higher than that in the control group（P<0.05）. The release of SDF-1 from the control group cells remained relatively stable，while the release of SDF-1 from the experimental group cells gradually increased over time and reached its peak on the 26th day. Subsequently，the release of SDF-1 gradually decreased over time. The relative mRNA expression levels of SDF-1 and CXCR4 in the experimental group cells were significantly higher than those in the control group cells detected by qPCR technology（P<0.05）. The ex-pression levels of SDF-1 and CXCR4 proteins in the experimental group cells were significantly higher than those in the control group cells by WB（P<0.05）.

Tripterygium wilfordii polysaccharides can induce pancreatic stem cell differentiation through the SDF-1/CXCR4 axis.

To study the effect of Jiawei Jiaotai Pill（加味交泰丸）in the treatment of irritable bowel syndrome based on bile acid metabolism and intestinal microecological abnormalities.

From August 2022 to June 2023，96 patients with diarrhea-predominant irritable bowel syndrome diagnosed and treated in the outpatient department of spleen and stomach disease department of nantong affiliated hospital of traditional Chinese medicine of Nanjing university of Chinese medicine were selected as the research objects. According to the random and control principle，they were divided into experimental group and control group，with 48 cases in each group. The patients of two groups were treated with routine treatment measures such as regulating emotions，reasonable diet and rest，and tobacco and alcohol control. At the same time，the control group was treated with Bacillus licheniformis and the experimental group was treated with Jiawei Jiaotai Pill. The course of treatment was 28 days and the treatment time of both groups was 1 course. The efficacy of TCM syndromes，irritable bowel syndrome symptom severity scale（IBS-SSS）score，Hamilton anxiety scale（HAMA）score，Hamilton depression scale（HAMD）score，bile acid level，intestinal flora distribution and treatment safety were compared between the two groups.

The total effective rate of the experimental group was 97.92%，which was significantly higher than that（83.33%）of the control group（P<0.05）. After treatment，the scores of IBS-SSS，HAMA and HAMD of the two groups were significantly decreased and the scores of the experimental group were significantly lower than those of the control group（P<0.05）. There was no significant difference in cholic acid（CA），che-nodeoxycholic acid（CDCA），ursodeoxycholic acid（UDCA），dehydrocholic acid（HDCA）or lithocholic acid（LCA）between the two groups before treatment（P>0.05）. After treatment，the levels of CA，CDCA and HDCA were significantly reduced，and the levels of UDCA and LCA were significantly increased. The improvement of the experimental group was better than that of the control group（P<0.05）. After treatment，the number of Bifidobacterium and Lactobacillus in the two groups increased，the number of Escherichia coli and Enterococcus decreased and the change in the experimental group was more significant（P<0.05）. There were no serious adverse reactions in the two groups and the treatment safety was good.

The clinical effect of Jiawei Jiaotai Pill in the treatment of diarrhea-predominant irritable bowel syndrome is satisfactory. It can significantly improve the bile acid metabolism and intestinal microecological abnormalities of patients，thereby alleviating their symptoms and promoting the rehabilitation of patients.

To study the effect of Longdan Xiegan Decoction（龙胆泻肝汤）combined with thunder-fire moxibustion in the treatment of chronic prostatitis/chronic pelvic pain syndrome.

A total of 160 patients with chronic prostatitis/chronic pelvic pain syndrome diagnosed and treated from January 2022 to December 2023 were selected as the research objects. According to the random number table method，they were randomly divided into four groups：western medicine group，western medicine+thunder fire moxibustion group，Chinese medicine group and Chinese medicine+thunder fire moxibustion group，with 40 cases in each group. The efficacy，pain symptom score（VAS），international prostate symptom score（IPSS），international erectile function score-5（IIEF-5），the maximum urinary flow rate（Qmax），urine volume（VV），prostate-specific extracellular vesicle protein（PSEP）and adverse reactions of the four groups were observed and compared.

The total effective rates of the western medicine group，the western medicine+thunder fire moxibustion group，the Chinese medicine group and the Chinese medicine+thunder fire moxibustion group were 80.00%（32/40），87.50%（35/40），77.50%（31/40）and 97.50%（39/40），respectively，and the differences between the groups were statistically significant（P<0.05）. After treatment，the VASand IPSS scores of the four groups decreased，and the IIEF-5 scores increased，and the scores of the traditional Chinese medicine+thunder fire moxibustion group changed more significantly（P<0.05）. There was no significant difference in Qmax，VV or PSEP among the four groups before treatment（P>0.05）. After treatment，the values of Qmax and VV of the four groups were significantly increased，and PSEPlevels were significantly decreased. The values of Qmax and VV in the Chinese medicine+thunder fire moxibustion group were the highest，and the PSEP level was the lowest. Compared with those of the other three groups，the difference was statistically significant（P<0.05）.

The effect of Longdan Xiegan Decoction combined with thunderfire moxibustion in the treatment of chronic prostatitis/chronic pelvic pain syndrome is significant. It can significantly relieve the symptoms of patients，improve their urination and sexual dysfunction，and has good safety and high application value.

To explore the effects of Wenyang Jieyu Decoction（温阳解郁汤）on serum inflammatory factors，monoamine neurotransmitter levels and intestinal microbiota in patients with post-stroke depression（PSD）.

Ninety PSD patients admitted to the hospital from January 2020 to March 2023 were selected and divided into control group（45 cases）and observation group（45 cases）by using a random number table method. The control group took Paroxetine Hydrochloride Tablets orally，while the observation group took Wenyang Jieyu Decoction on the basis of control group. The treatment period for two groups was 8 weeks. The therapeutic effects of two groups were compared. The changes in daily living ability，depressive mood，neurological function，inflammatory factors，monoamine neurotransmitters and gut microbiota were compared before and after treatment.

The effective rate of the observation group was higher than that of the control group（P<0.05）. After treatment，the daily activity level（ADL）scores of two groups were higher than those before treatment，while the Hamilton depression scale（HAMD）score and National Institute of Health stroke scale（NIHSS）score were lower than those before treatment（P<0.05）. The ADL score of the observation group was higher than that of the control group after treatment，while the HAMD score and NIHSSscore were lower than those of the control group（P<0.05）. After treatment，the levels of serum tumor necrosis factor-α（TNF-α），hypersensitive C-reactive protein（hs-CRP）and interleukin-6（IL-6）of two groups were lower than those before treatment（P<0.05）. And the levels of the observation group were lower than those of the control group（P<0.05）. After treatment，the levels of serum norepinephrine（NE），5-hydroxytryptamine，（5-HT）and dopamine（DA）of two groups were higher than those before treatment（P<0.05）and the levels of the observation group were higher than those of the control group（P<0.05）. After treatment，the levels of lactobacilli and bifidobacteria of two groups were higher than those before treatment，while the levels of Enterococcus and Escherichia coli were lower than those before treatment（P<0.05）. In the observation group，the levels of lactic acid bacteria and bifidobacteria were higher than those of the control group，while the levels of Enterococcus and Escherichia coli were lower than those of the control group（P<0.05）.

Wenyang Jieyu Decoction has a good therapeutic effect on PSD patients，which can significantly reduce inflammatory reactions，regulate monoamine neurotransmitter levels and improve intestinal microbiota changes.

To analyze the influence of Xixi Guzhe Prescription（西溪骨折方）on the prognosis of patients with tibiofibular fracture after operation and the serum type I collagen carboxy terminal peptideβspecial sequence（β-CTX）and collagen Crosslaps.

One hundred and ten patients with tibia and fibula fractures admitted to the hospital from June 2016 to March 2021 were randomly divided into the combined group（55 cases）and the control group（55 cases）. Both groups were treated with open reduction and internal fixation，and the combined group was treated with Xixi Guzhe Prescription. The changes of pain score，tenderness，swelling degree，serumβ-CTX and Crosslaps before and 7 days after operation，and the changes of knee joint function score and knee joint range of motion（ROM）in American Special Surgery Hospital（HSS）at 7 days and 4 weeks after operation were evaluated. The fracture healing time of the two groups was compared，and the prognosis of the two groups was evaluated by Johner-Wruh tibial shaft fracture evaluation standard at 4 weeks after operation.

The pain score，tenderness and swelling degree of the two groups decreased after 7 days，while the serumβ-CTX and Crosslaps increased. The pain score，tenderness and swelling degree of the combined group were lower than those of the control group after 7 days，while the serumβ-CTX and Crosslaps were higher than those of the control group（P<0.05）. The HSSscore and ROM of the two groups were higher than those of the control group at 4 weeks after operation，while those of the combined group were higher than those of the con-trol group at 4 weeks after operation（P<0.05）. The fracture healing time in the combined group was（15.14±2.63）weeks，which was lower than that in the control group（16.88±3.15）weeks（t＝3.145，P=0.002）. The excellent and good recovery rate in the combined group was 94.55%（52/55）after 4 weeks，which was higher than that in the control group（81.82%，45/55）（P<0.05）. The incidence of postoperative complications in the combined group and the control group were 7.27%（4/55）and 10.91%（6/55）respectively，and there was no statistical difference between the two groups（P>0.05）.

On the basis of open reduction and internal fixation，the addition of Xixi Guzhe Prescription can relieve patients'pain，improve bone metabolism and play a positive role in promoting the early rehabilitation of patients'knee joint function，and will not lead to an increase in the risk of postoperative complications.