Full
Outline
To investigate the mechanism of Tripterygium wilfordii polysaccharides inducing pancreatic stem cell differentiation through stromal cell-derived factor-1（SDF-1）/CXC chemokine receptor-4（CXCR4）axis.
Primary pancreatic stem cells were prepared from pancreatic tissue from Kunming mice on day 4 of neonatal birth. The immunohistochemistry method was used to identify the pancreatic stem cells. Isletβcells were identified by dithiazone（DTZ）staining. An inverted fluorescence microscope was used to photograph and record the cells at various cultivation time points. The release of SDF-1 from cells were detected at various administration time points by using enzyme-linked immunosorbent assay（ELISA）. The relative mRNA expression levels of SDF-1 and CXCR4 in each group were detected by real-time fluorescence quantitative PCR（qPCR）technology. The expression levels of SDF-1 and CXCR4 proteins in each group were detected by Western blot assay（WB）.
The cells obtained was Nestin positive and Brownish-yellow were identified by immunohistochemistry method. DTZ staining was positive，and the number of positive stained cells in the treatment group was significantly higher than that in the control group（P<0.05）. The release of SDF-1 from the control group cells remained relatively stable，while the release of SDF-1 from the experimental group cells gradually increased over time and reached its peak on the 26th day. Subsequently，the release of SDF-1 gradually decreased over time. The relative mRNA expression levels of SDF-1 and CXCR4 in the experimental group cells were significantly higher than those in the control group cells detected by qPCR technology（P<0.05）. The ex-pression levels of SDF-1 and CXCR4 proteins in the experimental group cells were significantly higher than those in the control group cells by WB（P<0.05）.
Tripterygium wilfordii polysaccharides can induce pancreatic stem cell differentiation through the SDF-1/CXCR4 axis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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