To observe the effects of volatile oil of Gaoliangjiang（Alpinia officinarum Hance），Dagaoliangjiang（Alpinia galanga Willd）and Hongdoukou（Alpinia galanga Willd）on body temperature and sympathetic nerve-adrenal axis in rats with stomach cold syndrome and to explore the material basis of warm properties of three Chinese drugs of Alpinia.

A total of 150 SPF grade SD male rats were selected，and 10 rats were randomly selected as the blank group.The other rats were simulated the stomach cold syndrome by oral administration of Zimu decoction.After modeling，they were randomly divided into model group，positive group，volatile oil of Gaoliangjiang（Alpinia officinarum Hance）high and low dose groups，volatile oil of Dagaoliangjiang（Alpinia galanga Willd）high and low dose groups，volatile oil of Hongdoukou（Alpinia galanga Willd）high and low dose groups，Gaoliangjiang（Alpinia officinarum Hance）high and low dose groups，Dagaoliangjiang（Alpinia galanga Willd）high and low dose groups，Hongdoukou（Alpinia galanga Willd）high and low dose groups.With gingerol as the positive control，the anal temperature was determined by electronic thermometer，the body temperature of upper abdomen and mid-abdomen was determined by infrared thermograph，and the contents of norepinephrine（NE），adrenocorticotropic hormone（ACTH），corticotropin releasing hormone（CRH），dopamine（DA），dopamine-β-hydroxylase（D-β-H），serotonin（5-HT），acetylcholinesterase（AchE）and 17 hydroxycorticosteroids（17-OHCS）were determined by ELISA.

Compared with those of the blank group，the contents of NE，ACTH，CRH，DA，D-β-H，AchE and 17-OHCSin serum of rats with stomach cold syndrome were significantly decreased.The content of 5-HT was significantly increased.Compared with that of the model group，the content of ACTH in all other groups，except that in the volatile oil of Dagaoliangjiang（Alpinia officinarum Hance）low dose group and Hongdoukou（Alpinia officinarum Hance）volatile oil groups，was significantly increased.The 17-OHCScontent was significantly increased in all groups except that in the low dose group of Gaoliangjiang（Alpinia officinarum Hance）.The CRH content of rats was significantly increased except that in low dose groups of Gaoliangjiang（Alpinia officinarum Hance）and Hongdoukou（Alpinia galanga Willd）.The D-β-H was significantly increased except that in low dose groups of Dagaoliangjiang（Alpinia galanga Willd）and Hongdoukou（Alpinia galanga Willd）.The DA content of rats was significantly increased and the 5-HTwas significantly decreased except those in the Hongdoukou（Alpinia galanga Willd）group.The AchE was significantly increased except that in the low dose group of Gaoliangjiang（Alpinia officinarum Hance）.The NE in each administration group was significantly increased.

The essential oil of three Chinese drugs of Alpinia can regulate the function of sympathetic nerve and adrenal axis in rats with stomach cold syndrome.

To evaluate the knowledge and clinical capabilities of large language models in the field of traditional Chinese medicine.

Using literature research and experimental research methods，9 different categories of large language models were selected，a data set of knowledge ability and clinical ability was constructed，and traditional Chinese medicine professional ability prompts were designed.The automatic evaluation method and expert scoring method were used to evaluate the large language models，and the variance was used.Analysis and multiple comparison methods were used to comparatively analyze the medical professional ability level of large language models.

In the evaluation of knowledge ability，Lingyi Wanwu（78.93），Zhipu Qingyan（77.91）and Tongyi Qianwen（77.22）performed best.There were significant differences in the knowledge ability scores of different models，different subjects and different types of models（P<0.05）.In the clinical capability evaluation，the responses generated by each model had high legibility（81.00），but low accuracy（74.86）.Among them，the average score of Wenxin Yiyan was the highest at 85.40，and all five-dimension scores reached excellent levels（≥80）.The difference in clinical ability scores of different types of models was significant.

The general large language model has advantages in generalization，legibility and security.The medical large language model performs well overall in the field of traditional Chinese medicine specialization and traditional Chinese medicine consultation.The application of large language models in the field of traditional Chinese medicine has broad development prospects in the future.

To investigate the effect and mechanism of Cynomorium songaricum flavonoids（CSF）on cognitive dysfunction in senescence-accelerated mice prone 8（SAMP8）.

Fifty 7-month-old male SAMP8 were randomly divided into control group，low，intermediate and high dose groups of CSF［25，50，100 mg/（kg·d）］，and donepezil group.The low，intermediate and high dose groups were given a corresponding dose of CSF to SAMP8 mice by continuous gavage for 20 days，while the donepezil group was given donepezil［0.71 mg/（kg·d）］to SAMP8 mice by gavage for 20 days.The spatial learning and memory performance was evaluate by the Morris water maze experiment after administration，and the morphology and count of hippocampal pyramidal cells was observed by Nissl staining.The apoptosis-related protein expression levels and the levels of nuclear factor E2 related factor 2（Nrf2），Kelch epichlorohydrin associated protein 1（Keap1）and quinone oxidoreductase-1（NQO1）in the hippocampus of each group were detected by Western blotting.The serum levels of malondialdehyde（MDA）content as well as glutathione peroxidase（GSH-Px）and superoxide dismutase（SOD）activities were measured by ELISA.

CSF significantly reduced the escape latency and path length，while increased the number of crossing platforms and the percentage of target quadrant time（P<0.05）.CSF improved the morphology and quantity of pyramidal cells in hippocampal CA1 region.Western blot results showed that compared with the control group，the protein expression levels of Nrf2，Keap1 and NQO1 in the hippocampus of the intermediate dose group，high dose group and donepezil group all significantly increased（P<0.05），the ratio of Bcl-2/Bax protein expression in the hippocampus of all administration groups of CSF and donepezil group increased substantially（P<0.05），and the expressions of Caspase-3 protein in the hippocampus of the intermediate dose group，high dose group and donepezil group all decreased significantly（P<0.05）.Compared with the control group，CSF significantly increased the serum activities of SOD and GSH-Px in SAMP8 mice（P<0.05），while remarkedly decreased the content of MDA（P<0.05）.

CSF can significantly improve spatial learning and memory impairment in Alzheimer＇s disease，and its mechanism may be related to inhibiting oxidative stress and cell apoptosis.

To observe the effects of acupuncture combined with neuromuscular electrical stimulation on shoulder joint function and nerve function after arthroscopic rotator cuff repair.

A hundred and fifty patients who visited the Affiliated Hospital of Henan University due to shoulder joint injury from December 2021 to December 2023 were selected as the research subjects.They were randomly divided into a control group and an observation group using a random number table method.The control group received neuromuscular electrical stimulation treatment，while the observation group received acupuncture combined with neuromuscular electrical stimulation.The excellent rate，adverse reactions，pain level，shoulder joint range of motion，shoulder joint function and nerve function were compared between the two groups at different time points before and after intervention.

The total effective rate of the observation group（96.00%，72/75）was significantly better than that of the control group（86.67%，65/75）（P<0.05）.At week 2，4 and 6 of intervention，the visual analogue scale（VAS）score of the shoulder joint in the observation group was significantly reduced（P<0.05，P<0.01）.After the intervention was completed，the active flexion angle and lateral rotation angle of the shoulder joint in the observation group were significantly increased compared to those in the control group（P<0.01），and the National Institutes of Health Stroke Scale（NIHSS）score of the observation group was significantly down regulated，while the American Shoulder and Elbow Surgeons’Form（ASES）score was significantly up regulated（P<0.01）.After 3 months of intervention，the 36-Item Short-Form（SF-36）score of the observation group patients was significantly up-regulated compared to that of the control group（P<0.01）.The incidence of adverse reactions in the observation group（12.00%，9/75）showed no significant difference compared to that in the control group（10.67%，8/75）（P>0.05）.

Acupuncture combined with neuromuscular electrical stimulation can significantly alleviate the postoperative pain in ARCR，expand the range of motion of the shoulder joint，promote the joint function recovery，improve the neurological function，reduce the shoulder muscle atrophy and enhance the quality of life.

To investigate the effect of Modified Baihe Gujin Decoction（加味百合固金汤）on CD⁺₄ T cell subsets and prognosis of patients with rifampicin-sensitive pulmonary tuberculosis of lung Yin deficiency syndrome.

A total of 94 patients with rifampicin-sensitive pulmonary tuberculosis of lung Yin deficiency syndrome admitted to the Tuberculosis Department of Zhengzhou Sixth People＇s Hospital from September 2020 to September 2023 were selected as the study objects，and were divided into study group and control group by simple random number table with 47 cases in each group.The control group received 2HRZE/4HRE anti-tuberculosis regimen.On the basis of the control group，the study group was treated with Modified Baihe Gujin Decoction for 2 months.TCM symptoms integral，immune function indexes［serum immunoglobulin G（IgG），immunoglobulin A（IgA），immunoglobulin M（IgM）］and CD⁺₄ T cell subsets were compared between the two groups before and after treatment.The lesion absorption，negative conversion rate and the occurrence of adverse reactions were compared during treatment.

After treatment，the scores of hot flash，dry throat，phlegm and night sweat in both groups were significantly decreased，and the score of the study group was significantly lower than that of the control group（P<0.05）.After treatment，the levels of IgG，IgA，IgM and CD⁺₄ T cell subsets were significantly increased in both groups，and those in the study group were significantly higher than those in the control group（P<0.05）.The lesion absorption rate and negative conversion rate of the study group were higher than those of the control group（P<0.05）.There was no significant difference in the incidence of adverse reactions between two groups（P>0.05）.

Modified Baihe Gujin Decoction is effective in the treatment of rifampicinsensitive pulmonary tuberculosis of lung Yin deficiency syndrome，which can significantly improve the immune function and prognosis of patients，and has high safety.

To analyze the therapeutic effect of Tianma Gouteng Yin（天麻钩藤饮）on hypertension and the mechanism of regulating mitochondrial protein pathway.

Ninety-two patients with hypertension admitted to the hospital from January 2023 to December 2023 were included as study objects，and were divided into two groups by random number table method.Forty-six patients in the control group were treated with nifedipine sustained-release tablet，and the other forty-six patients in the observation group were treated with Tianma Gouteng Yin on this basis.The curative effect，blood pressure level，symptom score，oxidative stress index and mitochondrial fusion protein level were compared between the two groups.

The total curative effect rate of the observation group was 95.65%（44/46），which was much higher than that of the control group（76.09%，35/46），and the difference was significant（P<0.05）.After treatment，the systolic blood pressure，diastolic blood pressure，vertigo，headache，lumbo-knee acid and five upset heat in the observation group were lower than those in the control group（P<0.05）.After treatment，the levels of glutathione peroxidase（GSH-Px）and superoxide dismutase（SOD）in the observation group were higher than those in the control group，the levels of malondialdehyde（MDA）and reactive oxygen species（ROS）were lower than those in the control group（P<0.05）.After treatment，the levels of recombinant mitofusin 1（MFN1），recombinant mitofusin 2（MFN2）and optic nerve atrophy protein 1（OPA1）in the observation group were higher than those in the control group（P<0.05）.

The application of Tianma Gouteng Yin in the treatment of hypertensive patients can effectively improve the symptoms of patients，reduce the level of blood pressure，delay the effect of vascular aging by enhancing the expression of mitochondrial fusion protein，reduce the oxidative stress reaction，improve the clinical efficacy，and has high application value.