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Research on Identification Method of Shicangpu(Acori Tatarinowii Rhizoma)and Common Counterfeits Based on Ultra-High Performance Liquid Chromatography
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Xianghua DENG1, 2, Dongmei SUN2, Xiaomei TAN1, Xiaozhou JIA2, Muxiang LUO1, 2, Tianrui XIA2, Guowei LI2, Xiangdong CHEN2
Chinese Archives of Traditional Chinese Medicine | 2025, 43(12) : 162 - 167
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Chinese Archives of Traditional Chinese Medicine | 2025, 43(12): 162-167
Target on National Project
Research on Identification Method of Shicangpu(Acori Tatarinowii Rhizoma)and Common Counterfeits Based on Ultra-High Performance Liquid Chromatography
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Xianghua DENG1, 2, Dongmei SUN2, Xiaomei TAN1, Xiaozhou JIA2, Muxiang LUO1, 2, Tianrui XIA2, Guowei LI2, Xiangdong CHEN2
Affiliations
  • 1.College of Traditional Chinese Medicine,Southern Medical University/Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Preparations,Guangzhou 510515,Guangdong,China
  • 2.Guangdong Yifang Pharmaceutical Co.,Ltd/Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,Guangdong,China
Published: 2025-12-10 doi: 10.13193/j.issn.1673-7717.2025.12.029
Outline
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Objective

To establish amethod for identifying Shicangpu(Acori Tatarinowii Rhizoma)(ATR)from common counterfeits by ultra performance liquid chromatography(UPLC)technology,and to provide reference for quality evaluation and control of ATR.

Methods

Waters CORTECST3 column(2.1 mm×150 mm,1.6μm)was used.Methanol:acetonitrile 1:1(A)-water(B)solution was used as mobile phase and gradientelution was performed.The flow ratewas0.2mL/min.The column temperature was40℃.The detection wavelength was275 nm and the sample volume was3μL.The UPLC characteristic map was established to identify ATR from common counterfeits.

Results

The UPLC characteristic map identified 4 common peaks of ATR and identified 1 of them.The similarity among samples was 0.892~0.996.

Conclusion

The established method of characteristic map is simple,reliable,accurate and repeatable,which can identify the authenticity of ATR directly,provide a specific method for the quality control and authenticity identification of ATR,and effectively improve the drug safety of related products.

Shichangpu(Acori Tatarinowii Schott)  /  Shuicangpu(Acorus calamus L.)  /  Huixiang Changpu(Acorus gramineus Soland)  /  Jiujie Changpu(Anemone altaica Fisch)  /  Yanbaicai[Bergenia purpurascens(Hook.f.et Thoms.)Engl.]  /  UPLC  /  identification
Xianghua DENG, Dongmei SUN, Xiaomei TAN, Xiaozhou JIA, Muxiang LUO, Tianrui XIA, Guowei LI, Xiangdong CHEN. Research on Identification Method of Shicangpu(Acori Tatarinowii Rhizoma)and Common Counterfeits Based on Ultra-High Performance Liquid Chromatography[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (12) : 162 -167 . DOI: 10.13193/j.issn.1673-7717.2025.12.029
Year 2025 volume 43 Issue 12
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doi: 10.13193/j.issn.1673-7717.2025.12.029
  • Online Date:2026-04-29
  • Published:2025-12-10
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    1.College of Traditional Chinese Medicine,Southern Medical University/Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Preparations,Guangzhou 510515,Guangdong,China
    2.Guangdong Yifang Pharmaceutical Co.,Ltd/Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,Guangdong,China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
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Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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