To establish amethod for identifying Shicangpu(Acori Tatarinowii Rhizoma)(ATR)from common counterfeits by ultra performance liquid chromatography(UPLC)technology,and to provide reference for quality evaluation and control of ATR.
Waters CORTECST3 column(2.1 mm×150 mm,1.6μm)was used.Methanol:acetonitrile 1:1(A)-water(B)solution was used as mobile phase and gradientelution was performed.The flow ratewas0.2mL/min.The column temperature was40℃.The detection wavelength was275 nm and the sample volume was3μL.The UPLC characteristic map was established to identify ATR from common counterfeits.
The UPLC characteristic map identified 4 common peaks of ATR and identified 1 of them.The similarity among samples was 0.892~0.996.
The established method of characteristic map is simple,reliable,accurate and repeatable,which can identify the authenticity of ATR directly,provide a specific method for the quality control and authenticity identification of ATR,and effectively improve the drug safety of related products.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |