To explore the neuroprotective effect of acteoside (ACT) on mouse model of MPTP-induced Parkinson's disease (PD) and its mechanism.

The PD mouse model was established by intraperitoneal injection of MPTP. The experimental mice were randomly divided into control group, model group and ACT group (100 mg·kg^-1), which were administrated by gavage for 14 d. After administration, behavioral evaluation and biochemical detection were performed to evaluate the neuroprotective effect of ACT on PD mice. Two-dimensional electrophoresis, mass spectrometry and Western blot were used to further explore the anti-PD mechanism of ACT.

After prophylactic administration of ACT, the pole climbing time of MPTP-induced PD mice was significantly shortened, swimming test score was improved, suspension duration was prolonged, and hind limb tension test score and cylinder test score were decreased. ACT could effectively improve the activities of SOD, CAT, and GSH-Px in the substantia nigra and striatum of MPTP-induced PD mice and reduce the content of MDA. The results of two-dimensional electrophoresis and Western blot showed that the expression of biliverdin reductase B (BLVRB) increased in the substantia nigra and striatum of PD mice induced by MPTP, while the expression of BLVRB decreased significantly after the administration of ACT.

ACT has neuroprotective effect on MPTP-induced PD mice, and its underlying mechanism may be related to the decrease of BLVRB expression and anti-oxidative stress of ACT.

To establish the first batch of national reference standard of rivaroxaban.

The structure of rivaroxaban was confirmed by IR, NMR and LC-MS spectroscopy. The purity of rivaroxaban was determined by HPLC. Moreover, moisture and residual ignition were determined. The content of rivaroxaban was calculated by mass balance method.

The structure of rivaroxaban was confirmed. The content of the first batch of national reference standard of rivaroxaban was assigned as 99.6%.

The first batch of national reference standard of rivaroxaban can be applied for the identification and content determination of rivaroxaban and its related preparations.

To establish a method for determination of polymer impurities in penicillin sodium for injection.

Penicillin sodium was dissolved by water to prepare penicillin polymer stock solution. High performance size exclusion chromatography method (HPSEC) and column switching-LC/MSn method were used to separate and deduce the weak retention impurities in polymer impurity solution and the specificity of HPSEC method was evaluated. A RP-HPLC method for polymer analysis was established with a C₁₈ column, using gradient elution by the mixed solution [phosphate buffer solution (pH 3.4)-methanol (72∶14)] and acetonitrile, and the RP-HPLC method was validated.

4 polymers, their isomers, one dimer degradation and some small molecular impurities were identified in the polymer impurity solution. Polymer impurities were liable to be co-eluted with small molecular impurities by HPSEC method, which resulted in poor quantification accuracy and specificity. 3 indicative polymer impurities were detected by RP-HPLC method with effective specificity, including 2 dimers produced by different polymerized patterns.

HPSEC cannot effectively control the polymer impurities of penicillin sodium for injection. The established RP-HPLC method has good specificity and high sensitivity for the determination of impurities in penicillin sodium polymer for injection.

To investigate the mechanism of compound AG-881 on inhibiting IDH1R132H mutant protein by molecular dynamics method.

The activated IDH1R132H protein structure and inactivated AG-881-IDH1R132H protein structure were constructed respectively and placed in a stereoscopic box. Molecular dynamics simulations were carried out on each system for 100 ns. The protein conformations of different systems were compared, and hydrogen bond analysis, energy analysis and principal component analysis (PCA), etc. were performed.

Substrate α-KG could stabilize IDH1R132H protein in the activated closed conformation. After AG-881 acted on the allosteric site of the protein at dimer interface, it made IDH1R132H in the open conformation of inactive state. In this process, amino acid residue Gln277 participated into the binding of AG-881. At the same time, hydrophobic occupation and hydrogen bond contributed mainly to the binding of AG-881 to IDH1R132H. In addition, halogen bond also played a certain role.

Molecular dynamics simulation can be used as an effective aid for allosteric inhibitor design, and the potential mechanism of AG-881 inhibiting IDH1R132H activity can be studied, which can further provide theoretical guidance for the development of new drugs targeting IDH1 mutant proteins.

To improve the quality standards of Mongolian medicine Shashen Zhike Decoction Powder.

TLC method was used to establish the identification of Bistortae Rhizoma and Shikonin, and the identification of Glycyrrhizae Radix et Rhizoma was revised. The content determination of glycyrrhizic acid was established by reversed phase HPLC.

The TLC identification methods resulted in clear spots of Bistortae Rhizoma, Shikonin and Glycyrrhizae Radix Et Rhizoma, without interference for the negative control. Glycyrrhizic acid had good linear relationship in the range from 92.90 to 1 161.25 ng (r=0.999 9). The average recovery was 102.57%, and the RSD was 1.85% (n=9).

The method is simple, specific, stable and repeatable, and can be used for the quality evaluation and control of Shashen Zhike Decoction Powder.