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To improve the quality standards of Mongolian medicine Shashen Zhike Decoction Powder.
TLC method was used to establish the identification of Bistortae Rhizoma and Shikonin, and the identification of Glycyrrhizae Radix et Rhizoma was revised. The content determination of glycyrrhizic acid was established by reversed phase HPLC.
The TLC identification methods resulted in clear spots of Bistortae Rhizoma, Shikonin and Glycyrrhizae Radix Et Rhizoma, without interference for the negative control. Glycyrrhizic acid had good linear relationship in the range from 92.90 to 1 161.25 ng (r=0.999 9). The average recovery was 102.57%, and the RSD was 1.85% (n=9).
The method is simple, specific, stable and repeatable, and can be used for the quality evaluation and control of Shashen Zhike Decoction Powder.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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