To evaluate the rationality of irinotecan application based on weighted TOPSIS method.

Based on the drug label of irinotecan, combined with relevant guidelines and literatures, weighted TOPSIS method was used to establish the evaluation criteria for the rationality of irinotecan clinical application, with “indications,contraindications, application and dosage, solvent selection, baseline examination, pretreatment, administration sequence,blood routine monitoring, dosage adjustment and ADR monitoring” as evaluation indicators. The archived medical records of inpatients who had used irinotecan in Binhu Hospital of Hefei from January 1, 2022 to May 31, 2023 were evaluated for the rationary of irinotecan administration.

Among the 10 evaluation indicators, the highest relative weighted index was indication（0.133 1）, while the lowest was pretreatment（0.084 9）. Among the included 97 cases, 10 cases（10%）had relative proximity（C_i）≥0.8, with the evaluation result as rational drug application; 79 cases（81%）had 0.6≤ C_i< 0.8,with the evaluation result as basic rational drug application; 8 cases（8%）had 0.4≤C_i < 0.6, with the evaluation result as irrational drug application.

The weighted TOPSIS method can be used to evaluate the rational usage of irinotecan. In our hospital, the use of irinotecan is basically reasonable, however, there are still some problems about baseline examination, blood routine monitoring and unreasonable indications.

To investigate the role of miR-17-5p in mediating cisplatin（DDP）resistance in breast cancer cells by regulating the PTEN/Akt pathway.

MCF-7/DDP-resistant cell line was cultured with a gradient of increasing DDP concentrations. The content of miR-17-5p was detected in MCF-7 and MCF-7/DDP cells by RT-qPCR. MCF-7 cells were divided into miR-NC and miR-17-5p groups, and transfected with miR-NC and miR-17-5p mimics plasmids,respectively. MCF-7/DDP-resistant cells were divided into anti-miR-NC and anti-miR-17-5p groups, and transfected with anti-miR-NC and anti-miR-17-5p plasmids, respectively. Transfection efficiency was defined by RT-qPCR. The drug sensitivity of DDP in each group of transfected cells was evaluated by MTT. The direct effect of miR-17-5p on the invasive ability was obtained by Transwell assay. DDP-induced apoptosis of MCF-7 and MCF-7/DDP cells after transfection was analyzed by flow cytometry. The targeting relationship between miR-17-5p and PTEN was verified by double luciferase reporter gene assay. The changes of apoptosis and key proteins of PTEN/Akt pathway under the regulation of miR-17-5p were detected by Western blot.

Compared with MCF-7 cells, the miR-17-5p expression in MCF-7/DDP-resistant cells was abnormally increased, while the PTEN expression was reduced（P<0.01）. PTEN was regulated by miR-17-5p as a target gene. Compared with the miR-NC group, the proliferation inhibition rate in the miR-17-5p mimics group was significantly declined, the number of invaded cells was enhanced, and the apoptosis rate was also decreased（P<0.05）, and the expressions of tumor suppressor proteins PTEN, p21 and p27 in the PTEN/Akt pathway were decreased, and the expressions of p-Akt308, p-Akt473 and cyclin D1 were increased（P<0.01）. Compared with the anti-miR-NC group, the proliferation inhibition rate was increased in the anti-miR-17-5p group, the number of invaded cells was decreased, and the apoptosis rate was also increased（P<0.05）, and the expression of tumor suppressor proteins PTEN, p21 and p27 was increased, and the expression of p-Akt308, p-Akt473 and cyclin D1 was decreased（P<0.01）.

Knockdown of miR-17-5p can effectively improve the DDP sensitivity of breast cancer cells, attenuate the invasive ability, and induce further apoptosis,which may be related to the regulatory effect of miR-17-5p on the PTEN/Akt pathway.

To explore the current research progress, research status, and future development directions in the field of traditional Chinese medicine（TCM）innovation.

CiteSpace software was used to analyze the paper trends and keywords in domestic journal articles between 1999 and 2023, and keyword clustering analysis, drawing timeline mapping,and emergent word analysis were used to visualize the field of Chinese medicine innovation in China.

The number of TCM innovation-related publications showed a fluctuating upward trend year by year. The research is still dominated by the researchers’ respective teams and lacks relevant team cooperation. The development of TCM research has entered a rapid development stage, which has envolved from the traditional research direction such as prescription research and development,it has developed to the construction of TCM data platform and intelligent production in the industry.

Research in the field of TCM innovation in China is developing steadily and undergoing a transformation towards diversification,digitization, and intelligence.

To investigate the analgesic mechanism of the active constituents of Piper wallichii.

The core targets related to analgesia of Piper wallichii were screened by network pharmacology, and GO functional enrichment and KEGG pathway enrichment analyses were carried out by DAVID database. The cationic elution site of Shinan vine was eluted by a silica gel column with a gradient（95:5-50:50）of petroleum ether-ethyl acetate separation system system to isolate the wallwort alkaloids, and the purity was determined by HPLC. Selected ICR mice were randomly divided into a blank group, positive control（aspirin 120 mg·kg^-1）group, a model group, and low, medium, and high dose（150, 300, and 600 mg·kg^-1）pellitorine groups（all n=8）, and were administered continuously for 7 d. After the last administration, each group was injected intraperitoneally with 0.1 mL of 0.7% acetic acid for every 10 g of body weight except the blank group. The number of twisting responses and latency period in each group were examined. Serum levels of tumour necrosis factor（TNF）-α, interleukin（IL）-6 and IL-1β were detected by ELISA, and the concentrations of choline acetyltransferase（ChAT）, acetylcholine（ACh）, and acetylcholinesterase（AChE）were examined in brain tissues.

The network pharmacological results showed that Piper wallichii screened out the core components such as pellitorine and futoamide, and the key pathways such as nerve signalling pathway and cholinergic synaptic pathway. The purity of isolated pellitorine was 90.42%. Animal experiments showed that, compared with the model group, the number of twisting in the low, medium and high dose pellitorine groups was significantly reduced（P<0.05）, and the content of TNF-α,IL-6 and IL-1β in serum was significantly reduced（P<0.01）. ChAT in the brain tissues of the three dose pellitorine groups were significantly higher（P<0.01）, and the concentration of ACh in the medium and high dose pellitorine groups were significantly higher（P<0.01）, and the AChE concentration of low, middle and high dose pellitorine groups were significantly decreased（P<0.05）.

The key active constituent in the analgesic effect of Piper wallichii is pellitorine, which is involved in the cholinergic synaptic pathway by up-regulating the activities of ChAT and ACh, and down-regulating AChE,thus reducing pain.