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To investigate the impact of erianin on the development of pancreatic cancer by regulating cyclic GMP-AMP synthase-stimulator of interferon gene (cGAS-STING) signal pathway.
SW1990 cells were divided into control group, low-, medium- and high-concentration erianin groups (10, 20, 40 nmol·L-1), RU.521 (cGAS inhibitor)group (1 μmol·L-1), and erianin (40 nmol·L-1) +RU.521 (1 μmol·L-1) group. The proliferation was detected by CCK-8 method, and apoptosis was detected by flow cytometry. The migration and invasion of SW1990 cells were detected by scratch test and Transwell test, respectively. Western blot was applied to detect the protein expressions of cyclin D1, Bcl-2 associated X protein (Bax), matrix metalloproteinase (MMP)-2, MMP-9, cGAS and STING in SW1990 cells. SW1990 cells were co-cultured with NK cells for 48 hours, the levels of granzyme B and perforin (PF) in co-culture supernatant were detected by ELISA, and NK cell killing activity was detected.
Compared with the control group, the cell proliferation rate,the rate of scratch healing, the number of invasive cells were decreased in the low-, medium- and high-concentration erianin groups, the protein expressions of cyclin D1, MMP-2, MMP-9 were decreased, the apoptosis rate was increased, and the protein expressions of Bax, cGAS and STING were decreased in a concentration-dependent manner (P<0.05), while the change trends of the above indexes in the RU.521 group were opposite (P<0.05). Compared with the high-concentration erianin group, the cell proliferation rate, scratch healing rate, the number of invasive cells, and the protein expressions of cyclin D1,MMP-2, MMP-9, Bax, cGAS and STING in the erianin +RU.521 group were increased, and the apoptosis rate was decreased (P<0.05). In the co-culture experiment, compared with the control group, the levels of granzyme B and PF and NK cell killing activity in the cell supernatant of the low-, medium- and high-concentration erianin groups were increased in a concentration-dependent manner (P<0.05), while the change trends of corresponding indexes in the RU.521 group were opposite (P<0.05).Compared with the high-concentration erianin group, the levels of granzyme B and PF and NK cell killing activity in the erianin+RU.521 group were decreased (P<0.05).
Erianin may inhibit the proliferation, migration, invasion,and immune escape of pancreatic cancer cells and promote cell apoptosis by activating cGAS-STING signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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